Intestinal expression of pro-inflammatory cytokines induced by oral intake of lipopolysaccharide (LPS) from E. coli in weaned pigs
ABSTRACT: treatments with lipopolysaccharide (LPS) from E. coli are an accepted way of inducing inflammation in immunological studies since they have the ability to activate a coordinate series of signs through the synthesis of pro-inflammatory cytokines such as interleukin 8 (IL-8), IL-18 and tumor...
- Autores:
-
Agudelo Trujillo, Jorge Hernán
Sanin Peña, David Esteban
- Tipo de recurso:
- Article of investigation
- Fecha de publicación:
- 2012
- Institución:
- Universidad de Antioquia
- Repositorio:
- Repositorio UdeA
- Idioma:
- eng
- OAI Identifier:
- oai:bibliotecadigital.udea.edu.co:10495/8070
- Acceso en línea:
- http://hdl.handle.net/10495/8070
- Palabra clave:
- Gut immunology
Intestinal villi
Pig weaning
RT-PCR, TNF-α
- Rights
- openAccess
- License
- Atribución-NoComercial-CompartirIgual 2.5 Colombia (CC BY-NC-SA 2.5 CO)
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dc.title.spa.fl_str_mv |
Intestinal expression of pro-inflammatory cytokines induced by oral intake of lipopolysaccharide (LPS) from E. coli in weaned pigs |
dc.title.alternative.spa.fl_str_mv |
Expresión intestinal de citoquinas proinflamatorias inducidas mediante ingestión de lipopolisacáridos de E. coli en lechones recién destetados |
title |
Intestinal expression of pro-inflammatory cytokines induced by oral intake of lipopolysaccharide (LPS) from E. coli in weaned pigs |
spellingShingle |
Intestinal expression of pro-inflammatory cytokines induced by oral intake of lipopolysaccharide (LPS) from E. coli in weaned pigs Gut immunology Intestinal villi Pig weaning RT-PCR, TNF-α |
title_short |
Intestinal expression of pro-inflammatory cytokines induced by oral intake of lipopolysaccharide (LPS) from E. coli in weaned pigs |
title_full |
Intestinal expression of pro-inflammatory cytokines induced by oral intake of lipopolysaccharide (LPS) from E. coli in weaned pigs |
title_fullStr |
Intestinal expression of pro-inflammatory cytokines induced by oral intake of lipopolysaccharide (LPS) from E. coli in weaned pigs |
title_full_unstemmed |
Intestinal expression of pro-inflammatory cytokines induced by oral intake of lipopolysaccharide (LPS) from E. coli in weaned pigs |
title_sort |
Intestinal expression of pro-inflammatory cytokines induced by oral intake of lipopolysaccharide (LPS) from E. coli in weaned pigs |
dc.creator.fl_str_mv |
Agudelo Trujillo, Jorge Hernán Sanin Peña, David Esteban |
dc.contributor.author.none.fl_str_mv |
Agudelo Trujillo, Jorge Hernán Sanin Peña, David Esteban |
dc.subject.none.fl_str_mv |
Gut immunology Intestinal villi Pig weaning RT-PCR, TNF-α |
topic |
Gut immunology Intestinal villi Pig weaning RT-PCR, TNF-α |
description |
ABSTRACT: treatments with lipopolysaccharide (LPS) from E. coli are an accepted way of inducing inflammation in immunological studies since they have the ability to activate a coordinate series of signs through the synthesis of pro-inflammatory cytokines such as interleukin 8 (IL-8), IL-18 and tumor necrosis factor alpha (TNF-α), which can cause significant changes in intestinal structure and functionality. Objective: the aim of this study was to evaluate the effect of adding LPS of E. coli on pro-inflammatory cytokine gene expression IL-8, IL-18 and TNF-α in early-weaned pigs. Methods: fieldwork was conducted at Centro San Pablo, belonging to the Universidad Nacional de Colombia with 32 pigs at 21 days of age, with 6.5 ± 0.5 kg of weight. Animals were fed with a basal diet supplemented with two levels of inclusion of LPS of E. coli serotype 0111:B4 (0 to 0.3 μg/mg of food). Pigs were slaughtered in stages on days 1, 5, 7 and 10 postweaning and complete extraction of small intestine was made. Gene expression was evaluated by qPCR. Blocks at random in a factorial arrangement 2 x 4 were used as statistical design. Results: the basal diet (without addition of LPS) presented increase in mRNA expression (p<0.01) of all the cytokines in jejunum for each post-weaning day, which suggests an inflammatory response and extensive tissue damage in pigs after early weaning. In diet 1 (with consumption of 0.3 μg LPS / mg diet) cytokines TNF-α, IL-8 and IL- 18 showed a significant increase in their levels of expression (p<0.01). All cytokines presented significant increase (p<0.01) in jejunum for each post-weaning day. Conclusion: The increase observed in the expression of TNF-α can be involved in the development of post-weaning diarrhea. |
publishDate |
2012 |
dc.date.issued.none.fl_str_mv |
2012 |
dc.date.accessioned.none.fl_str_mv |
2017-08-30T19:16:13Z |
dc.date.available.none.fl_str_mv |
2017-08-30T19:16:13Z |
dc.type.spa.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.coarversion.fl_str_mv |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
dc.type.hasversion.spa.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.coar.spa.fl_str_mv |
http://purl.org/coar/resource_type/c_2df8fbb1 |
dc.type.redcol.spa.fl_str_mv |
https://purl.org/redcol/resource_type/ART |
dc.type.local.spa.fl_str_mv |
Artículo de investigación |
format |
http://purl.org/coar/resource_type/c_2df8fbb1 |
status_str |
publishedVersion |
dc.identifier.citation.spa.fl_str_mv |
Agudelo Trujillo JH, Sanin P D. Intestinal expression of pro-inflammatory cytokines induced by oral intake of lipopolysaccharide (LPS) from E. coli in weaned pigs. Rev. Colomb. Cienc. Pecu. 2012;26(2): 108-118. |
dc.identifier.issn.none.fl_str_mv |
0120-0690 |
dc.identifier.uri.none.fl_str_mv |
http://hdl.handle.net/10495/8070 |
dc.identifier.eissn.none.fl_str_mv |
2256-2958 |
identifier_str_mv |
Agudelo Trujillo JH, Sanin P D. Intestinal expression of pro-inflammatory cytokines induced by oral intake of lipopolysaccharide (LPS) from E. coli in weaned pigs. Rev. Colomb. Cienc. Pecu. 2012;26(2): 108-118. 0120-0690 2256-2958 |
url |
http://hdl.handle.net/10495/8070 |
dc.language.iso.spa.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartofjournalabbrev.spa.fl_str_mv |
Rev. Colomb. Cienc. Pecu. |
dc.rights.*.fl_str_mv |
Atribución-NoComercial-CompartirIgual 2.5 Colombia (CC BY-NC-SA 2.5 CO) |
dc.rights.spa.fl_str_mv |
info:eu-repo/semantics/openAccess |
dc.rights.uri.*.fl_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/co/ |
dc.rights.accessrights.spa.fl_str_mv |
http://purl.org/coar/access_right/c_abf2 |
dc.rights.creativecommons.spa.fl_str_mv |
https://creativecommons.org/licenses/by-nc-sa/4.0/ |
rights_invalid_str_mv |
Atribución-NoComercial-CompartirIgual 2.5 Colombia (CC BY-NC-SA 2.5 CO) https://creativecommons.org/licenses/by-nc-sa/2.5/co/ http://purl.org/coar/access_right/c_abf2 https://creativecommons.org/licenses/by-nc-sa/4.0/ |
eu_rights_str_mv |
openAccess |
dc.format.mimetype.spa.fl_str_mv |
application/pdf |
dc.publisher.spa.fl_str_mv |
Universidad de Antioquia, Facultad de Ciencias Agrarias |
dc.publisher.place.spa.fl_str_mv |
Medellín, Colombia |
institution |
Universidad de Antioquia |
bitstream.url.fl_str_mv |
http://bibliotecadigital.udea.edu.co/bitstream/10495/8070/1/SaninDavid_2013_IntestinalExpressionInfammatory.pdf http://bibliotecadigital.udea.edu.co/bitstream/10495/8070/2/license_url http://bibliotecadigital.udea.edu.co/bitstream/10495/8070/3/license_text http://bibliotecadigital.udea.edu.co/bitstream/10495/8070/4/license_rdf http://bibliotecadigital.udea.edu.co/bitstream/10495/8070/5/license.txt |
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Agudelo Trujillo, Jorge HernánSanin Peña, David Esteban2017-08-30T19:16:13Z2017-08-30T19:16:13Z2012Agudelo Trujillo JH, Sanin P D. Intestinal expression of pro-inflammatory cytokines induced by oral intake of lipopolysaccharide (LPS) from E. coli in weaned pigs. Rev. Colomb. Cienc. Pecu. 2012;26(2): 108-118.0120-0690http://hdl.handle.net/10495/80702256-2958ABSTRACT: treatments with lipopolysaccharide (LPS) from E. coli are an accepted way of inducing inflammation in immunological studies since they have the ability to activate a coordinate series of signs through the synthesis of pro-inflammatory cytokines such as interleukin 8 (IL-8), IL-18 and tumor necrosis factor alpha (TNF-α), which can cause significant changes in intestinal structure and functionality. Objective: the aim of this study was to evaluate the effect of adding LPS of E. coli on pro-inflammatory cytokine gene expression IL-8, IL-18 and TNF-α in early-weaned pigs. Methods: fieldwork was conducted at Centro San Pablo, belonging to the Universidad Nacional de Colombia with 32 pigs at 21 days of age, with 6.5 ± 0.5 kg of weight. Animals were fed with a basal diet supplemented with two levels of inclusion of LPS of E. coli serotype 0111:B4 (0 to 0.3 μg/mg of food). Pigs were slaughtered in stages on days 1, 5, 7 and 10 postweaning and complete extraction of small intestine was made. Gene expression was evaluated by qPCR. Blocks at random in a factorial arrangement 2 x 4 were used as statistical design. Results: the basal diet (without addition of LPS) presented increase in mRNA expression (p<0.01) of all the cytokines in jejunum for each post-weaning day, which suggests an inflammatory response and extensive tissue damage in pigs after early weaning. In diet 1 (with consumption of 0.3 μg LPS / mg diet) cytokines TNF-α, IL-8 and IL- 18 showed a significant increase in their levels of expression (p<0.01). All cytokines presented significant increase (p<0.01) in jejunum for each post-weaning day. Conclusion: The increase observed in the expression of TNF-α can be involved in the development of post-weaning diarrhea.RESUMEN: los tratamientos con lipopolisacáridos (LPS) de E. coli son una forma aceptada para inducción de inflamación en estudios inmunológicos ya que tienen la capacidad de activar una gran variedad de rutas de señalización a través de la síntesis de citoquinas proinflamatorias, tales como interleuquina 8 (IL- 8), IL-18 y factor de necrosis tumoral alfa (TNF-α), las cuales pueden provocar cambios importantes en la estructura y funcionalidad intestinal. Objetivo: el presente estudio tuvo como objetivo evaluar el efecto de la adición de LPS de E. coli sobre la expresión génica de las citoquinas proinflamatorias IL-8, IL-18, y TNF-α en lechones recién destetados. Métodos: este trabajo se realizó con 32 lechones de 21 días de edad, con un peso de 6,5 ± 0,5 kg en el Centro San Pablo, perteneciente a la Universidad Nacional de Colombia. Los animales fueron alimentados con una dieta basal adicionada con dos niveles de inclusión de LPS de E. coli serotipo 0111:B4 (0 y 0,3 μg/mg de alimento). Los animales se sacrificaron escalonadamente los días 1, 5, 7 y 10 posdestete, y se realizó extracción completa del intestino delgado. Se evaluó la expresión génica por qPCR. El diseño estadístico empleado fue de bloques al azar en un arreglo factorial de 2X4. Resultados: la dieta basal (sin adición de LPS) presentó incremento en la expresión de mRNA (p<0,01) de todas las citoquinas en yeyuno para cada día posdestete, lo que sugiere una respuesta inflamatoria y grandes daños tisulares en el lechón luego del destete precoz. En la dieta 1 (con consumo de 0,3 µg LPS /mg dieta) las citoquinas TNF-α, IL-8, e IL-18 presentaron incrementos significativos en sus niveles de expresión (p<0,01). Todas las citoquinas presentaron incremento significativo (p<0,01) en yeyuno para cada uno de los días posdestete. Conclusión: el incremento observado en la expresión de TNF-α puede estar implicado en el desarrollo de diarreas posdestete.application/pdfengUniversidad de Antioquia, Facultad de Ciencias AgrariasMedellín, Colombiainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_2df8fbb1https://purl.org/redcol/resource_type/ARTArtículo de investigaciónhttp://purl.org/coar/version/c_970fb48d4fbd8a85Atribución-NoComercial-CompartirIgual 2.5 Colombia (CC BY-NC-SA 2.5 CO)info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/co/http://purl.org/coar/access_right/c_abf2https://creativecommons.org/licenses/by-nc-sa/4.0/Gut immunologyIntestinal villiPig weaningRT-PCR, TNF-αIntestinal expression of pro-inflammatory cytokines induced by oral intake of lipopolysaccharide (LPS) from E. coli in weaned pigsExpresión intestinal de citoquinas proinflamatorias inducidas mediante ingestión de lipopolisacáridos de E. coli en lechones recién destetadosRev. Colomb. Cienc. Pecu.Revista Colombiana de Ciencias Pecuarias108118262ORIGINALSaninDavid_2013_IntestinalExpressionInfammatory.pdfSaninDavid_2013_IntestinalExpressionInfammatory.pdfArtículo de investigaciónapplication/pdf357994http://bibliotecadigital.udea.edu.co/bitstream/10495/8070/1/SaninDavid_2013_IntestinalExpressionInfammatory.pdf46a619f66272760720cc4f079e0a4903MD51CC-LICENSElicense_urllicense_urltext/plain; charset=utf-849http://bibliotecadigital.udea.edu.co/bitstream/10495/8070/2/license_url4afdbb8c545fd630ea7db775da747b2fMD52license_textlicense_texttext/html; charset=utf-80http://bibliotecadigital.udea.edu.co/bitstream/10495/8070/3/license_textd41d8cd98f00b204e9800998ecf8427eMD53license_rdflicense_rdfapplication/rdf+xml; charset=utf-80http://bibliotecadigital.udea.edu.co/bitstream/10495/8070/4/license_rdfd41d8cd98f00b204e9800998ecf8427eMD54LICENSElicense.txtlicense.txttext/plain; charset=utf-81748http://bibliotecadigital.udea.edu.co/bitstream/10495/8070/5/license.txt8a4605be74aa9ea9d79846c1fba20a33MD5510495/8070oai:bibliotecadigital.udea.edu.co:10495/80702021-05-21 20:29:21.377Repositorio Institucional Universidad de Antioquiaandres.perez@udea.edu.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 |