Detection of hilA Gene Sequences in Serovars of Salmonella enterica Subspecies Enterica

ABSTRACT : HilA gene promoter, component of the Salmonella Pathogenicity Island 1, has been found in Salmonella serovar Typhimurium, being important for the regulation of type III secretion apparatus genes. We detected hilA gene sequences in Salmonella serovars Typhi, Enteritidis, Choleraesuis, Para...

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Autores:
Cardona Castro, Nora
Restrepo Pineda, Eliana
Correa Ochoa, Margarita María
Tipo de recurso:
Article of investigation
Fecha de publicación:
2002
Institución:
Universidad de Antioquia
Repositorio:
Repositorio UdeA
Idioma:
eng
OAI Identifier:
oai:bibliotecadigital.udea.edu.co:10495/27734
Acceso en línea:
http://hdl.handle.net/10495/27734
Palabra clave:
Salmonella enteritidis
Salmonella paratyphi A
Salmonella paratyphi B
Salmonella arizonae
Reacción en Cadena de la Polimerasa
Polymerase Chain Reaction
Antígenos de Pullorum
Pollorum antigens
hilA gene sequences
Rights
openAccess
License
http://creativecommons.org/licenses/by/2.5/co/
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oai_identifier_str oai:bibliotecadigital.udea.edu.co:10495/27734
network_acronym_str UDEA2
network_name_str Repositorio UdeA
repository_id_str
dc.title.spa.fl_str_mv Detection of hilA Gene Sequences in Serovars of Salmonella enterica Subspecies Enterica
title Detection of hilA Gene Sequences in Serovars of Salmonella enterica Subspecies Enterica
spellingShingle Detection of hilA Gene Sequences in Serovars of Salmonella enterica Subspecies Enterica
Salmonella enteritidis
Salmonella paratyphi A
Salmonella paratyphi B
Salmonella arizonae
Reacción en Cadena de la Polimerasa
Polymerase Chain Reaction
Antígenos de Pullorum
Pollorum antigens
hilA gene sequences
title_short Detection of hilA Gene Sequences in Serovars of Salmonella enterica Subspecies Enterica
title_full Detection of hilA Gene Sequences in Serovars of Salmonella enterica Subspecies Enterica
title_fullStr Detection of hilA Gene Sequences in Serovars of Salmonella enterica Subspecies Enterica
title_full_unstemmed Detection of hilA Gene Sequences in Serovars of Salmonella enterica Subspecies Enterica
title_sort Detection of hilA Gene Sequences in Serovars of Salmonella enterica Subspecies Enterica
dc.creator.fl_str_mv Cardona Castro, Nora
Restrepo Pineda, Eliana
Correa Ochoa, Margarita María
dc.contributor.author.none.fl_str_mv Cardona Castro, Nora
Restrepo Pineda, Eliana
Correa Ochoa, Margarita María
dc.subject.decs.none.fl_str_mv Salmonella enteritidis
Salmonella paratyphi A
Salmonella paratyphi B
Salmonella arizonae
Reacción en Cadena de la Polimerasa
Polymerase Chain Reaction
topic Salmonella enteritidis
Salmonella paratyphi A
Salmonella paratyphi B
Salmonella arizonae
Reacción en Cadena de la Polimerasa
Polymerase Chain Reaction
Antígenos de Pullorum
Pollorum antigens
hilA gene sequences
dc.subject.lemb.none.fl_str_mv Antígenos de Pullorum
Pollorum antigens
dc.subject.proposal.spa.fl_str_mv hilA gene sequences
description ABSTRACT : HilA gene promoter, component of the Salmonella Pathogenicity Island 1, has been found in Salmonella serovar Typhimurium, being important for the regulation of type III secretion apparatus genes. We detected hilA gene sequences in Salmonella serovars Typhi, Enteritidis, Choleraesuis, Paratyphi A and B, and Pullorum, by polymerase chain reaction (PCR) and hybridization techniques. The primers to carry out PCR were designed according to hilA sequence. A low stringency hybridization with the probe pVV441 (hilA open-readingframe plasmid) was carried out. To find hilA gene sequences in other Salmonella sp. suggest that these serovars could have similar sequences of this kind of virulence genes.
publishDate 2002
dc.date.issued.none.fl_str_mv 2002
dc.date.accessioned.none.fl_str_mv 2022-04-22T23:46:24Z
dc.date.available.none.fl_str_mv 2022-04-22T23:46:24Z
dc.type.spa.fl_str_mv info:eu-repo/semantics/article
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dc.type.local.spa.fl_str_mv Artículo de investigación
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status_str publishedVersion
dc.identifier.issn.none.fl_str_mv 0074-0276
dc.identifier.uri.none.fl_str_mv http://hdl.handle.net/10495/27734
dc.identifier.doi.none.fl_str_mv 10.1590/S0074-02762002000800016
dc.identifier.eissn.none.fl_str_mv 1678-8060
identifier_str_mv 0074-0276
10.1590/S0074-02762002000800016
1678-8060
url http://hdl.handle.net/10495/27734
dc.language.iso.spa.fl_str_mv eng
language eng
dc.relation.ispartofjournalabbrev.spa.fl_str_mv Mem. Inst. Oswaldo Cruz.
dc.rights.spa.fl_str_mv info:eu-repo/semantics/openAccess
dc.rights.uri.*.fl_str_mv http://creativecommons.org/licenses/by/2.5/co/
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dc.format.extent.spa.fl_str_mv 4
dc.format.mimetype.spa.fl_str_mv application/pdf
dc.publisher.spa.fl_str_mv Instituto Oswaldo Cruz
dc.publisher.group.spa.fl_str_mv Microbiología Molecular
dc.publisher.place.spa.fl_str_mv Río de Janeiro, Brasil
institution Universidad de Antioquia
bitstream.url.fl_str_mv http://bibliotecadigital.udea.edu.co/bitstream/10495/27734/1/CorreaMargarita_2002_DetectionHilASalmonella.pdf
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repository.name.fl_str_mv Repositorio Institucional Universidad de Antioquia
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spelling Cardona Castro, NoraRestrepo Pineda, ElianaCorrea Ochoa, Margarita María2022-04-22T23:46:24Z2022-04-22T23:46:24Z20020074-0276http://hdl.handle.net/10495/2773410.1590/S0074-027620020008000161678-8060ABSTRACT : HilA gene promoter, component of the Salmonella Pathogenicity Island 1, has been found in Salmonella serovar Typhimurium, being important for the regulation of type III secretion apparatus genes. We detected hilA gene sequences in Salmonella serovars Typhi, Enteritidis, Choleraesuis, Paratyphi A and B, and Pullorum, by polymerase chain reaction (PCR) and hybridization techniques. The primers to carry out PCR were designed according to hilA sequence. A low stringency hybridization with the probe pVV441 (hilA open-readingframe plasmid) was carried out. To find hilA gene sequences in other Salmonella sp. suggest that these serovars could have similar sequences of this kind of virulence genes.COL00137464application/pdfengInstituto Oswaldo CruzMicrobiología MolecularRío de Janeiro, Brasilinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_2df8fbb1https://purl.org/redcol/resource_type/ARTArtículo de investigaciónhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/2.5/co/http://purl.org/coar/access_right/c_abf2https://creativecommons.org/licenses/by/4.0/Detection of hilA Gene Sequences in Serovars of Salmonella enterica Subspecies EntericaSalmonella enteritidisSalmonella paratyphi ASalmonella paratyphi BSalmonella arizonaeReacción en Cadena de la PolimerasaPolymerase Chain ReactionAntígenos de PullorumPollorum antigenshilA gene sequencesMem. Inst. Oswaldo Cruz.Memórias do Instituto Oswaldo Cruz11531156978ORIGINALCorreaMargarita_2002_DetectionHilASalmonella.pdfCorreaMargarita_2002_DetectionHilASalmonella.pdfArtículo de investigaciónapplication/pdf64522http://bibliotecadigital.udea.edu.co/bitstream/10495/27734/1/CorreaMargarita_2002_DetectionHilASalmonella.pdf7bf43afab112b055e3cd0b53f653e5d7MD51CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8927http://bibliotecadigital.udea.edu.co/bitstream/10495/27734/2/license_rdf1646d1f6b96dbbbc38035efc9239ac9cMD52LICENSElicense.txtlicense.txttext/plain; charset=utf-81748http://bibliotecadigital.udea.edu.co/bitstream/10495/27734/3/license.txt8a4605be74aa9ea9d79846c1fba20a33MD5310495/27734oai:bibliotecadigital.udea.edu.co:10495/277342022-04-22 18:46:25.465Repositorio Institucional Universidad de Antioquiaandres.perez@udea.edu.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