Detection of hilA Gene Sequences in Serovars of Salmonella enterica Subspecies Enterica

ABSTRACT : HilA gene promoter, component of the Salmonella Pathogenicity Island 1, has been found in Salmonella serovar Typhimurium, being important for the regulation of type III secretion apparatus genes. We detected hilA gene sequences in Salmonella serovars Typhi, Enteritidis, Choleraesuis, Paratyphi A and B, and Pullorum, by polymerase chain reaction (PCR) and hybridization techniques. The primers to carry out PCR were designed according to hilA sequence. A low stringency hybridization with the probe pVV441 (hilA open-readingframe plasmid) was carried out. To find hilA gene sequences in other Salmonella sp. suggest that these serovars could have similar sequences of this kind of virulence genes.

• Autores: Cardona Castro, Nora; Restrepo Pineda, Eliana; Correa Ochoa, Margarita María
• Tipo de recurso: Article of investigation
• Fecha de publicación: 2002
• Institución: Universidad de Antioquia
• Repositorio: Repositorio UdeA
• Idioma: eng
• OAI Identifier: oai:bibliotecadigital.udea.edu.co:10495/27734
• Acceso en línea: http://hdl.handle.net/10495/27734
• Palabra clave: Salmonella enteritidis; Salmonella paratyphi A; Salmonella paratyphi B; Salmonella arizonae; Reacción en Cadena de la Polimerasa; Polymerase Chain Reaction; Antígenos de Pullorum; Pollorum antigens; hilA gene sequences
• Rights: openAccess
• License: http://creativecommons.org/licenses/by/2.5/co/