Glycosylation profile of the transferrin receptor in gestational iron deficiency and early-onset severe preeclampsia
ABSTRACT: To examine the expression of hypoxia-inducible factor-1α (HIF-1α), TfR1, and TfR1-attached terminal monosaccharides in placentas of women with IDAP and severe preeclampsia. Methods. TfR1 and HIF-1α were detected by western blot. Immunoadsorption of TfR1 was performed to characterize the te...
- Autores:
-
Gómez Gutiérrez, Alejandra María
Parra Sosa, Beatriz Elena
Bueno Sánchez, Julio César
- Tipo de recurso:
- Article of investigation
- Fecha de publicación:
- 2019
- Institución:
- Universidad de Antioquia
- Repositorio:
- Repositorio UdeA
- Idioma:
- eng
- OAI Identifier:
- oai:bibliotecadigital.udea.edu.co:10495/12009
- Acceso en línea:
- http://hdl.handle.net/10495/12009
- Palabra clave:
- Rights
- openAccess
- License
- Atribución-NoComercial-SinDerivadas 2.5 Colombia
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Glycosylation profile of the transferrin receptor in gestational iron deficiency and early-onset severe preeclampsia |
| title |
Glycosylation profile of the transferrin receptor in gestational iron deficiency and early-onset severe preeclampsia |
| spellingShingle |
Glycosylation profile of the transferrin receptor in gestational iron deficiency and early-onset severe preeclampsia |
| title_short |
Glycosylation profile of the transferrin receptor in gestational iron deficiency and early-onset severe preeclampsia |
| title_full |
Glycosylation profile of the transferrin receptor in gestational iron deficiency and early-onset severe preeclampsia |
| title_fullStr |
Glycosylation profile of the transferrin receptor in gestational iron deficiency and early-onset severe preeclampsia |
| title_full_unstemmed |
Glycosylation profile of the transferrin receptor in gestational iron deficiency and early-onset severe preeclampsia |
| title_sort |
Glycosylation profile of the transferrin receptor in gestational iron deficiency and early-onset severe preeclampsia |
| dc.creator.fl_str_mv |
Gómez Gutiérrez, Alejandra María Parra Sosa, Beatriz Elena Bueno Sánchez, Julio César |
| dc.contributor.author.none.fl_str_mv |
Gómez Gutiérrez, Alejandra María Parra Sosa, Beatriz Elena Bueno Sánchez, Julio César |
| description |
ABSTRACT: To examine the expression of hypoxia-inducible factor-1α (HIF-1α), TfR1, and TfR1-attached terminal monosaccharides in placentas of women with IDAP and severe preeclampsia. Methods. TfR1 and HIF-1α were detected by western blot. Immunoadsorption of TfR1 was performed to characterize the terminal monosaccharides by specific lectin binding. Results. There was no difference in the expression of TfR1 and HIF-1α between groups. Lectin blot analysis pointed out an overexpression of galactose β1-4 N-acetylglucosamine (Gal-GlcNAc) and mannose in severe preeclampsia. Conclusion. The increase in Gal-GlcNAc may be due to the increased presence of antennary structures and the mannose glycans of TfR1 may indicate the presence of misfolded or incomplete proteins. These findings may be associated with the low expression of placental TfR1 in women with preeclampsia. |
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2019 |
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2019-09-19T16:43:14Z |
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2019-09-19T16:43:14Z |
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2019 |
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http://purl.org/coar/version/c_970fb48d4fbd8a85 |
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Gómez-Gutiérrez AM, Parra-Sosa BE, Bueno-Sánchez JC. Glycosylation profile of the transferrin receptor in gestational iron deficiency and early-onset severe preeclampsia. Journal of Pregnancy. 2019; 9514546. DOI: 10.1155/2019/9514546 |
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2090-2727 |
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http://hdl.handle.net/10495/12009 |
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10.1155/2019/9514546 |
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2090-2735 |
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Gómez-Gutiérrez AM, Parra-Sosa BE, Bueno-Sánchez JC. Glycosylation profile of the transferrin receptor in gestational iron deficiency and early-onset severe preeclampsia. Journal of Pregnancy. 2019; 9514546. DOI: 10.1155/2019/9514546 2090-2727 10.1155/2019/9514546 2090-2735 |
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http://hdl.handle.net/10495/12009 |
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eng |
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eng |
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Journal of Pregnancy J. Pregnancy |
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Atribución-NoComercial-SinDerivadas 2.5 Colombia |
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Alimentación y Nutrición Humana Grupo Reproducción |
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El Cairo, Egipto |
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Universidad de Antioquia |
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Gómez Gutiérrez, Alejandra MaríaParra Sosa, Beatriz ElenaBueno Sánchez, Julio César2019-09-19T16:43:14Z2019-09-19T16:43:14Z2019Gómez-Gutiérrez AM, Parra-Sosa BE, Bueno-Sánchez JC. Glycosylation profile of the transferrin receptor in gestational iron deficiency and early-onset severe preeclampsia. Journal of Pregnancy. 2019; 9514546. DOI: 10.1155/2019/95145462090-2727http://hdl.handle.net/10495/1200910.1155/2019/95145462090-2735ABSTRACT: To examine the expression of hypoxia-inducible factor-1α (HIF-1α), TfR1, and TfR1-attached terminal monosaccharides in placentas of women with IDAP and severe preeclampsia. Methods. TfR1 and HIF-1α were detected by western blot. Immunoadsorption of TfR1 was performed to characterize the terminal monosaccharides by specific lectin binding. Results. There was no difference in the expression of TfR1 and HIF-1α between groups. Lectin blot analysis pointed out an overexpression of galactose β1-4 N-acetylglucosamine (Gal-GlcNAc) and mannose in severe preeclampsia. Conclusion. The increase in Gal-GlcNAc may be due to the increased presence of antennary structures and the mannose glycans of TfR1 may indicate the presence of misfolded or incomplete proteins. These findings may be associated with the low expression of placental TfR1 in women with preeclampsia.COL0000407COL0007631application/pdfengHindawiAlimentación y Nutrición HumanaGrupo ReproducciónEl Cairo, Egiptoinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_2df8fbb1https://purl.org/redcol/resource_type/ARTArtículo de investigaciónhttp://purl.org/coar/version/c_970fb48d4fbd8a85Atribución-NoComercial-SinDerivadas 2.5 Colombiainfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-nd/2.5/co/http://purl.org/coar/access_right/c_abf2https://creativecommons.org/licenses/by-nc-nd/4.0/Glycosylation profile of the transferrin receptor in gestational iron deficiency and early-onset severe preeclampsiaJournal of PregnancyJ. PregnancyJournal of Pregnancy19CC-LICENSElicense_urllicense_urltext/plain; charset=utf-849http://bibliotecadigital.udea.edu.co/bitstream/10495/12009/2/license_url4afdbb8c545fd630ea7db775da747b2fMD52license_textlicense_texttext/html; charset=utf-80http://bibliotecadigital.udea.edu.co/bitstream/10495/12009/3/license_textd41d8cd98f00b204e9800998ecf8427eMD53license_rdflicense_rdfLicenciaapplication/rdf+xml; charset=utf-80http://bibliotecadigital.udea.edu.co/bitstream/10495/12009/4/license_rdfd41d8cd98f00b204e9800998ecf8427eMD54LICENSElicense.txtlicense.txttext/plain; charset=utf-81748http://bibliotecadigital.udea.edu.co/bitstream/10495/12009/5/license.txt8a4605be74aa9ea9d79846c1fba20a33MD55ORIGINALGomezAlejandra_2019_GlycosylationProfileTransferrinReceptor.pdfGomezAlejandra_2019_GlycosylationProfileTransferrinReceptor.pdfArtículo de investigaciónapplication/pdf1961278http://bibliotecadigital.udea.edu.co/bitstream/10495/12009/1/GomezAlejandra_2019_GlycosylationProfileTransferrinReceptor.pdf6d11b78284eeb5bcd8bc50edc61d823aMD5110495/12009oai:bibliotecadigital.udea.edu.co:10495/120092021-10-25 13:38:10.173Repositorio Institucional Universidad de Antioquiaandres.perez@udea.edu.coTk9URTogUExBQ0UgWU9VUiBPV04gTElDRU5TRSBIRVJFClRoaXMgc2FtcGxlIGxpY2Vuc2UgaXMgcHJvdmlkZWQgZm9yIGluZm9ybWF0aW9uYWwgcHVycG9zZXMgb25seS4KCk5PTi1FWENMVVNJVkUgRElTVFJJQlVUSU9OIExJQ0VOU0UKCkJ5IHNpZ25pbmcgYW5kIHN1Ym1pdHRpbmcgdGhpcyBsaWNlbnNlLCB5b3UgKHRoZSBhdXRob3Iocykgb3IgY29weXJpZ2h0Cm93bmVyKSBncmFudHMgdG8gRFNwYWNlIFVuaXZlcnNpdHkgKERTVSkgdGhlIG5vbi1leGNsdXNpdmUgcmlnaHQgdG8gcmVwcm9kdWNlLAp0cmFuc2xhdGUgKGFzIGRlZmluZWQgYmVsb3cpLCBhbmQvb3IgZGlzdHJpYnV0ZSB5b3VyIHN1Ym1pc3Npb24gKGluY2x1ZGluZwp0aGUgYWJzdHJhY3QpIHdvcmxkd2lkZSBpbiBwcmludCBhbmQgZWxlY3Ryb25pYyBmb3JtYXQgYW5kIGluIGFueSBtZWRpdW0sCmluY2x1ZGluZyBidXQgbm90IGxpbWl0ZWQgdG8gYXVkaW8gb3IgdmlkZW8uCgpZb3UgYWdyZWUgdGhhdCBEU1UgbWF5LCB3aXRob3V0IGNoYW5naW5nIHRoZSBjb250ZW50LCB0cmFuc2xhdGUgdGhlCnN1Ym1pc3Npb24gdG8gYW55IG1lZGl1bSBvciBmb3JtYXQgZm9yIHRoZSBwdXJwb3NlIG9mIHByZXNlcnZhdGlvbi4KCllvdSBhbHNvIGFncmVlIHRoYXQgRFNVIG1heSBrZWVwIG1vcmUgdGhhbiBvbmUgY29weSBvZiB0aGlzIHN1Ym1pc3Npb24gZm9yCnB1cnBvc2VzIG9mIHNlY3VyaXR5LCBiYWNrLXVwIGFuZCBwcmVzZXJ2YXRpb24uCgpZb3UgcmVwcmVzZW50IHRoYXQgdGhlIHN1Ym1pc3Npb24gaXMgeW91ciBvcmlnaW5hbCB3b3JrLCBhbmQgdGhhdCB5b3UgaGF2ZQp0aGUgcmlnaHQgdG8gZ3JhbnQgdGhlIHJpZ2h0cyBjb250YWluZWQgaW4gdGhpcyBsaWNlbnNlLiBZb3UgYWxzbyByZXByZXNlbnQKdGhhdCB5b3VyIHN1Ym1pc3Npb24gZG9lcyBub3QsIHRvIHRoZSBiZXN0IG9mIHlvdXIga25vd2xlZGdlLCBpbmZyaW5nZSB1cG9uCmFueW9uZSdzIGNvcHlyaWdodC4KCklmIHRoZSBzdWJtaXNzaW9uIGNvbnRhaW5zIG1hdGVyaWFsIGZvciB3aGljaCB5b3UgZG8gbm90IGhvbGQgY29weXJpZ2h0LAp5b3UgcmVwcmVzZW50IHRoYXQgeW91IGhhdmUgb2J0YWluZWQgdGhlIHVucmVzdHJpY3RlZCBwZXJtaXNzaW9uIG9mIHRoZQpjb3B5cmlnaHQgb3duZXIgdG8gZ3JhbnQgRFNVIHRoZSByaWdodHMgcmVxdWlyZWQgYnkgdGhpcyBsaWNlbnNlLCBhbmQgdGhhdApzdWNoIHRoaXJkLXBhcnR5IG93bmVkIG1hdGVyaWFsIGlzIGNsZWFybHkgaWRlbnRpZmllZCBhbmQgYWNrbm93bGVkZ2VkCndpdGhpbiB0aGUgdGV4dCBvciBjb250ZW50IG9mIHRoZSBzdWJtaXNzaW9uLgoKSUYgVEhFIFNVQk1JU1NJT04gSVMgQkFTRUQgVVBPTiBXT1JLIFRIQVQgSEFTIEJFRU4gU1BPTlNPUkVEIE9SIFNVUFBPUlRFRApCWSBBTiBBR0VOQ1kgT1IgT1JHQU5JWkFUSU9OIE9USEVSIFRIQU4gRFNVLCBZT1UgUkVQUkVTRU5UIFRIQVQgWU9VIEhBVkUKRlVMRklMTEVEIEFOWSBSSUdIVCBPRiBSRVZJRVcgT1IgT1RIRVIgT0JMSUdBVElPTlMgUkVRVUlSRUQgQlkgU1VDSApDT05UUkFDVCBPUiBBR1JFRU1FTlQuCgpEU1Ugd2lsbCBjbGVhcmx5IGlkZW50aWZ5IHlvdXIgbmFtZShzKSBhcyB0aGUgYXV0aG9yKHMpIG9yIG93bmVyKHMpIG9mIHRoZQpzdWJtaXNzaW9uLCBhbmQgd2lsbCBub3QgbWFrZSBhbnkgYWx0ZXJhdGlvbiwgb3RoZXIgdGhhbiBhcyBhbGxvd2VkIGJ5IHRoaXMKbGljZW5zZSwgdG8geW91ciBzdWJtaXNzaW9uLgo= |