Leishmanicidal and cytotoxic activity of extracts and saponins from Ilex laurina (Aquifoliaceae)
ABSTRACT: Purpose: To evaluate the leishmanicidal and cytotoxic activity of alcohol and non-alcohol extracts and saponins from Ilex laurina. Methods: Extracts were obtained by percolation with solvents of different polarities: hexane, dichloromethane, ethyl acetate and ethanol. The ethyl acetate ext...
- Autores:
-
Cardona Galeano, Wilson
Pérez Cardona, Juan Manuel
Robledo Restrepo, Sara María
Alzate Guarín, Fernando
Muñoz Herrera, Diana Lorena
Herrera Ramírez, Angie
- Tipo de recurso:
- Article of investigation
- Fecha de publicación:
- 2016
- Institución:
- Universidad de Antioquia
- Repositorio:
- Repositorio UdeA
- Idioma:
- eng
- OAI Identifier:
- oai:bibliotecadigital.udea.edu.co:10495/30995
- Acceso en línea:
- https://hdl.handle.net/10495/30995
https://www.ajol.info/index.php/tjpr/article/view/136341
- Palabra clave:
- Leishmaniosis
Antiprotozoarios
Antiprotozoal Agents
Saponinas
Saponins
Aquifoliáceas
Aquifoliaceae
llex laurina
- Rights
- openAccess
- License
- http://creativecommons.org/licenses/by/2.5/co/
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|
dc.title.spa.fl_str_mv |
Leishmanicidal and cytotoxic activity of extracts and saponins from Ilex laurina (Aquifoliaceae) |
dc.title.alternative.spa.fl_str_mv |
Actividad leishmanicida y citotóxica de extractos y saponinas de Ilex laurina (Aquifoliaceae) |
title |
Leishmanicidal and cytotoxic activity of extracts and saponins from Ilex laurina (Aquifoliaceae) |
spellingShingle |
Leishmanicidal and cytotoxic activity of extracts and saponins from Ilex laurina (Aquifoliaceae) Leishmaniosis Antiprotozoarios Antiprotozoal Agents Saponinas Saponins Aquifoliáceas Aquifoliaceae llex laurina |
title_short |
Leishmanicidal and cytotoxic activity of extracts and saponins from Ilex laurina (Aquifoliaceae) |
title_full |
Leishmanicidal and cytotoxic activity of extracts and saponins from Ilex laurina (Aquifoliaceae) |
title_fullStr |
Leishmanicidal and cytotoxic activity of extracts and saponins from Ilex laurina (Aquifoliaceae) |
title_full_unstemmed |
Leishmanicidal and cytotoxic activity of extracts and saponins from Ilex laurina (Aquifoliaceae) |
title_sort |
Leishmanicidal and cytotoxic activity of extracts and saponins from Ilex laurina (Aquifoliaceae) |
dc.creator.fl_str_mv |
Cardona Galeano, Wilson Pérez Cardona, Juan Manuel Robledo Restrepo, Sara María Alzate Guarín, Fernando Muñoz Herrera, Diana Lorena Herrera Ramírez, Angie |
dc.contributor.author.none.fl_str_mv |
Cardona Galeano, Wilson Pérez Cardona, Juan Manuel Robledo Restrepo, Sara María Alzate Guarín, Fernando Muñoz Herrera, Diana Lorena Herrera Ramírez, Angie |
dc.subject.decs.none.fl_str_mv |
Leishmaniosis Antiprotozoarios Antiprotozoal Agents Saponinas Saponins |
topic |
Leishmaniosis Antiprotozoarios Antiprotozoal Agents Saponinas Saponins Aquifoliáceas Aquifoliaceae llex laurina |
dc.subject.lemb.none.fl_str_mv |
Aquifoliáceas Aquifoliaceae |
dc.subject.proposal.spa.fl_str_mv |
llex laurina |
description |
ABSTRACT: Purpose: To evaluate the leishmanicidal and cytotoxic activity of alcohol and non-alcohol extracts and saponins from Ilex laurina. Methods: Extracts were obtained by percolation with solvents of different polarities: hexane, dichloromethane, ethyl acetate and ethanol. The ethyl acetate extract was subjected to silica gel column chromatography eluting with a step gradient of dichloromethane-methanol. All products were evaluated in vitro for leishmanicidal activity against amastigotes of leishmania panamensis and cytotoxicity on U937 cells. Results: Two saponins were isolated from the ethyl acetate extract. The ethyl acetate extract showed high leishmanicidal activity against intracellular amastigotes of L. panamensis (EC50, 7.5 ± 1.5 µg/mL) and low activity against axenic amastigotes (EC50, 52.8 ±1.6 µg/mL); this extract showed also high cytotoxicity (LC50, 57.7 ± 12.1 µg/mL). Saponin 2 exhibited high activity against intracellular amastigotes (EC50, 5.9 ± 0.5 µg/mL) but also showed high cytotoxicity on U-937 cells (EC50, 25.7 ± 6.1 µg/mL). This compound showed similar leishmanicidal activity and cytotoxicity to meglumine antimoniate and amphotericin B, respectively, drugs currently used for the treatment of leishmaniasis. Conclusions: Based on these results, Ilex laurina is a potential source of compounds that can lead to the development of new therapeutic alternatives against leishmaniasis. |
publishDate |
2016 |
dc.date.issued.none.fl_str_mv |
2016 |
dc.date.accessioned.none.fl_str_mv |
2022-09-30T17:58:22Z |
dc.date.available.none.fl_str_mv |
2022-09-30T17:58:22Z |
dc.type.spa.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.coarversion.fl_str_mv |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
dc.type.hasversion.spa.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.coar.spa.fl_str_mv |
http://purl.org/coar/resource_type/c_2df8fbb1 |
dc.type.redcol.spa.fl_str_mv |
https://purl.org/redcol/resource_type/ART |
dc.type.local.spa.fl_str_mv |
Artículo de investigación |
format |
http://purl.org/coar/resource_type/c_2df8fbb1 |
status_str |
publishedVersion |
dc.identifier.issn.none.fl_str_mv |
1596-5996 |
dc.identifier.uri.none.fl_str_mv |
https://hdl.handle.net/10495/30995 |
dc.identifier.doi.none.fl_str_mv |
10.4314/tjpr.v15i5.11 |
dc.identifier.eissn.none.fl_str_mv |
1596-9827 |
dc.identifier.url.spa.fl_str_mv |
https://www.ajol.info/index.php/tjpr/article/view/136341 |
identifier_str_mv |
1596-5996 10.4314/tjpr.v15i5.11 1596-9827 |
url |
https://hdl.handle.net/10495/30995 https://www.ajol.info/index.php/tjpr/article/view/136341 |
dc.language.iso.spa.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartofjournalabbrev.spa.fl_str_mv |
Trop. J. Pharm. Res |
dc.rights.spa.fl_str_mv |
info:eu-repo/semantics/openAccess |
dc.rights.uri.*.fl_str_mv |
http://creativecommons.org/licenses/by/2.5/co/ |
dc.rights.accessrights.spa.fl_str_mv |
http://purl.org/coar/access_right/c_abf2 |
dc.rights.creativecommons.spa.fl_str_mv |
https://creativecommons.org/licenses/by/4.0/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by/2.5/co/ http://purl.org/coar/access_right/c_abf2 https://creativecommons.org/licenses/by/4.0/ |
dc.format.extent.spa.fl_str_mv |
7 |
dc.format.mimetype.spa.fl_str_mv |
application/pdf |
dc.publisher.spa.fl_str_mv |
University of Benin, Faculty of Pharmacy |
dc.publisher.group.spa.fl_str_mv |
Grupo de Estudios Botánicos Programa de Estudio y Control de Enfermedades Tropicales (PECET) Química de Plantas Colombianas |
dc.publisher.place.spa.fl_str_mv |
Ciudad de Benín, Nigeria |
institution |
Universidad de Antioquia |
bitstream.url.fl_str_mv |
https://bibliotecadigital.udea.edu.co/bitstream/10495/30995/2/license_rdf https://bibliotecadigital.udea.edu.co/bitstream/10495/30995/1/CardonaWilson_2016_LeishmanicidalCytotoxicActivityOfExtracts.pdf https://bibliotecadigital.udea.edu.co/bitstream/10495/30995/3/license.txt |
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MD5 MD5 MD5 |
repository.name.fl_str_mv |
Repositorio Institucional Universidad de Antioquia |
repository.mail.fl_str_mv |
andres.perez@udea.edu.co |
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1812173164329828352 |
spelling |
Cardona Galeano, WilsonPérez Cardona, Juan ManuelRobledo Restrepo, Sara MaríaAlzate Guarín, FernandoMuñoz Herrera, Diana LorenaHerrera Ramírez, Angie2022-09-30T17:58:22Z2022-09-30T17:58:22Z20161596-5996https://hdl.handle.net/10495/3099510.4314/tjpr.v15i5.111596-9827https://www.ajol.info/index.php/tjpr/article/view/136341ABSTRACT: Purpose: To evaluate the leishmanicidal and cytotoxic activity of alcohol and non-alcohol extracts and saponins from Ilex laurina. Methods: Extracts were obtained by percolation with solvents of different polarities: hexane, dichloromethane, ethyl acetate and ethanol. The ethyl acetate extract was subjected to silica gel column chromatography eluting with a step gradient of dichloromethane-methanol. All products were evaluated in vitro for leishmanicidal activity against amastigotes of leishmania panamensis and cytotoxicity on U937 cells. Results: Two saponins were isolated from the ethyl acetate extract. The ethyl acetate extract showed high leishmanicidal activity against intracellular amastigotes of L. panamensis (EC50, 7.5 ± 1.5 µg/mL) and low activity against axenic amastigotes (EC50, 52.8 ±1.6 µg/mL); this extract showed also high cytotoxicity (LC50, 57.7 ± 12.1 µg/mL). Saponin 2 exhibited high activity against intracellular amastigotes (EC50, 5.9 ± 0.5 µg/mL) but also showed high cytotoxicity on U-937 cells (EC50, 25.7 ± 6.1 µg/mL). This compound showed similar leishmanicidal activity and cytotoxicity to meglumine antimoniate and amphotericin B, respectively, drugs currently used for the treatment of leishmaniasis. Conclusions: Based on these results, Ilex laurina is a potential source of compounds that can lead to the development of new therapeutic alternatives against leishmaniasis.COL0015329COL0015099COL00082277application/pdfengUniversity of Benin, Faculty of PharmacyGrupo de Estudios BotánicosPrograma de Estudio y Control de Enfermedades Tropicales (PECET)Química de Plantas ColombianasCiudad de Benín, Nigeriainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_2df8fbb1https://purl.org/redcol/resource_type/ARTArtículo de investigaciónhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/2.5/co/http://purl.org/coar/access_right/c_abf2https://creativecommons.org/licenses/by/4.0/Leishmanicidal and cytotoxic activity of extracts and saponins from Ilex laurina (Aquifoliaceae)Actividad leishmanicida y citotóxica de extractos y saponinas de Ilex laurina (Aquifoliaceae)LeishmaniosisAntiprotozoariosAntiprotozoal AgentsSaponinasSaponinsAquifoliáceasAquifoliaceaellex laurinaTrop. J. Pharm. ResTropical Journal of Pharmaceutical973979155CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8927https://bibliotecadigital.udea.edu.co/bitstream/10495/30995/2/license_rdf1646d1f6b96dbbbc38035efc9239ac9cMD52ORIGINALCardonaWilson_2016_LeishmanicidalCytotoxicActivityOfExtracts.pdfCardonaWilson_2016_LeishmanicidalCytotoxicActivityOfExtracts.pdfArtículo de investigaciónapplication/pdf208736https://bibliotecadigital.udea.edu.co/bitstream/10495/30995/1/CardonaWilson_2016_LeishmanicidalCytotoxicActivityOfExtracts.pdfe90e7f8a0d98dfe0237128304c5197ecMD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://bibliotecadigital.udea.edu.co/bitstream/10495/30995/3/license.txt8a4605be74aa9ea9d79846c1fba20a33MD5310495/30995oai:bibliotecadigital.udea.edu.co:10495/309952022-09-30 12:58:23.387Repositorio Institucional Universidad de Antioquiaandres.perez@udea.edu.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 |