Leishmanicidal and cytotoxic activity of extracts and saponins from Ilex laurina (Aquifoliaceae)

ABSTRACT: Purpose: To evaluate the leishmanicidal and cytotoxic activity of alcohol and non-alcohol extracts and saponins from Ilex laurina. Methods: Extracts were obtained by percolation with solvents of different polarities: hexane, dichloromethane, ethyl acetate and ethanol. The ethyl acetate ext...

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Autores:
Cardona Galeano, Wilson
Pérez Cardona, Juan Manuel
Robledo Restrepo, Sara María
Alzate Guarín, Fernando
Muñoz Herrera, Diana Lorena
Herrera Ramírez, Angie
Tipo de recurso:
Article of investigation
Fecha de publicación:
2016
Institución:
Universidad de Antioquia
Repositorio:
Repositorio UdeA
Idioma:
eng
OAI Identifier:
oai:bibliotecadigital.udea.edu.co:10495/30995
Acceso en línea:
https://hdl.handle.net/10495/30995
https://www.ajol.info/index.php/tjpr/article/view/136341
Palabra clave:
Leishmaniosis
Antiprotozoarios
Antiprotozoal Agents
Saponinas
Saponins
Aquifoliáceas
Aquifoliaceae
llex laurina
Rights
openAccess
License
http://creativecommons.org/licenses/by/2.5/co/
id UDEA2_e51186d673e8c632c996afb4c60e36ee
oai_identifier_str oai:bibliotecadigital.udea.edu.co:10495/30995
network_acronym_str UDEA2
network_name_str Repositorio UdeA
repository_id_str
dc.title.spa.fl_str_mv Leishmanicidal and cytotoxic activity of extracts and saponins from Ilex laurina (Aquifoliaceae)
dc.title.alternative.spa.fl_str_mv Actividad leishmanicida y citotóxica de extractos y saponinas de Ilex laurina (Aquifoliaceae)
title Leishmanicidal and cytotoxic activity of extracts and saponins from Ilex laurina (Aquifoliaceae)
spellingShingle Leishmanicidal and cytotoxic activity of extracts and saponins from Ilex laurina (Aquifoliaceae)
Leishmaniosis
Antiprotozoarios
Antiprotozoal Agents
Saponinas
Saponins
Aquifoliáceas
Aquifoliaceae
llex laurina
title_short Leishmanicidal and cytotoxic activity of extracts and saponins from Ilex laurina (Aquifoliaceae)
title_full Leishmanicidal and cytotoxic activity of extracts and saponins from Ilex laurina (Aquifoliaceae)
title_fullStr Leishmanicidal and cytotoxic activity of extracts and saponins from Ilex laurina (Aquifoliaceae)
title_full_unstemmed Leishmanicidal and cytotoxic activity of extracts and saponins from Ilex laurina (Aquifoliaceae)
title_sort Leishmanicidal and cytotoxic activity of extracts and saponins from Ilex laurina (Aquifoliaceae)
dc.creator.fl_str_mv Cardona Galeano, Wilson
Pérez Cardona, Juan Manuel
Robledo Restrepo, Sara María
Alzate Guarín, Fernando
Muñoz Herrera, Diana Lorena
Herrera Ramírez, Angie
dc.contributor.author.none.fl_str_mv Cardona Galeano, Wilson
Pérez Cardona, Juan Manuel
Robledo Restrepo, Sara María
Alzate Guarín, Fernando
Muñoz Herrera, Diana Lorena
Herrera Ramírez, Angie
dc.subject.decs.none.fl_str_mv Leishmaniosis
Antiprotozoarios
Antiprotozoal Agents
Saponinas
Saponins
topic Leishmaniosis
Antiprotozoarios
Antiprotozoal Agents
Saponinas
Saponins
Aquifoliáceas
Aquifoliaceae
llex laurina
dc.subject.lemb.none.fl_str_mv Aquifoliáceas
Aquifoliaceae
dc.subject.proposal.spa.fl_str_mv llex laurina
description ABSTRACT: Purpose: To evaluate the leishmanicidal and cytotoxic activity of alcohol and non-alcohol extracts and saponins from Ilex laurina. Methods: Extracts were obtained by percolation with solvents of different polarities: hexane, dichloromethane, ethyl acetate and ethanol. The ethyl acetate extract was subjected to silica gel column chromatography eluting with a step gradient of dichloromethane-methanol. All products were evaluated in vitro for leishmanicidal activity against amastigotes of leishmania panamensis and cytotoxicity on U937 cells. Results: Two saponins were isolated from the ethyl acetate extract. The ethyl acetate extract showed high leishmanicidal activity against intracellular amastigotes of L. panamensis (EC50, 7.5 ± 1.5 µg/mL) and low activity against axenic amastigotes (EC50, 52.8 ±1.6 µg/mL); this extract showed also high cytotoxicity (LC50, 57.7 ± 12.1 µg/mL). Saponin 2 exhibited high activity against intracellular amastigotes (EC50, 5.9 ± 0.5 µg/mL) but also showed high cytotoxicity on U-937 cells (EC50, 25.7 ± 6.1 µg/mL). This compound showed similar leishmanicidal activity and cytotoxicity to meglumine antimoniate and amphotericin B, respectively, drugs currently used for the treatment of leishmaniasis. Conclusions: Based on these results, Ilex laurina is a potential source of compounds that can lead to the development of new therapeutic alternatives against leishmaniasis.
publishDate 2016
dc.date.issued.none.fl_str_mv 2016
dc.date.accessioned.none.fl_str_mv 2022-09-30T17:58:22Z
dc.date.available.none.fl_str_mv 2022-09-30T17:58:22Z
dc.type.spa.fl_str_mv info:eu-repo/semantics/article
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dc.type.hasversion.spa.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.coar.spa.fl_str_mv http://purl.org/coar/resource_type/c_2df8fbb1
dc.type.redcol.spa.fl_str_mv https://purl.org/redcol/resource_type/ART
dc.type.local.spa.fl_str_mv Artículo de investigación
format http://purl.org/coar/resource_type/c_2df8fbb1
status_str publishedVersion
dc.identifier.issn.none.fl_str_mv 1596-5996
dc.identifier.uri.none.fl_str_mv https://hdl.handle.net/10495/30995
dc.identifier.doi.none.fl_str_mv 10.4314/tjpr.v15i5.11
dc.identifier.eissn.none.fl_str_mv 1596-9827
dc.identifier.url.spa.fl_str_mv https://www.ajol.info/index.php/tjpr/article/view/136341
identifier_str_mv 1596-5996
10.4314/tjpr.v15i5.11
1596-9827
url https://hdl.handle.net/10495/30995
https://www.ajol.info/index.php/tjpr/article/view/136341
dc.language.iso.spa.fl_str_mv eng
language eng
dc.relation.ispartofjournalabbrev.spa.fl_str_mv Trop. J. Pharm. Res
dc.rights.spa.fl_str_mv info:eu-repo/semantics/openAccess
dc.rights.uri.*.fl_str_mv http://creativecommons.org/licenses/by/2.5/co/
dc.rights.accessrights.spa.fl_str_mv http://purl.org/coar/access_right/c_abf2
dc.rights.creativecommons.spa.fl_str_mv https://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/2.5/co/
http://purl.org/coar/access_right/c_abf2
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dc.format.extent.spa.fl_str_mv 7
dc.format.mimetype.spa.fl_str_mv application/pdf
dc.publisher.spa.fl_str_mv University of Benin, Faculty of Pharmacy
dc.publisher.group.spa.fl_str_mv Grupo de Estudios Botánicos
Programa de Estudio y Control de Enfermedades Tropicales (PECET)
Química de Plantas Colombianas
dc.publisher.place.spa.fl_str_mv Ciudad de Benín, Nigeria
institution Universidad de Antioquia
bitstream.url.fl_str_mv https://bibliotecadigital.udea.edu.co/bitstream/10495/30995/2/license_rdf
https://bibliotecadigital.udea.edu.co/bitstream/10495/30995/1/CardonaWilson_2016_LeishmanicidalCytotoxicActivityOfExtracts.pdf
https://bibliotecadigital.udea.edu.co/bitstream/10495/30995/3/license.txt
bitstream.checksum.fl_str_mv 1646d1f6b96dbbbc38035efc9239ac9c
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bitstream.checksumAlgorithm.fl_str_mv MD5
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repository.name.fl_str_mv Repositorio Institucional Universidad de Antioquia
repository.mail.fl_str_mv andres.perez@udea.edu.co
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spelling Cardona Galeano, WilsonPérez Cardona, Juan ManuelRobledo Restrepo, Sara MaríaAlzate Guarín, FernandoMuñoz Herrera, Diana LorenaHerrera Ramírez, Angie2022-09-30T17:58:22Z2022-09-30T17:58:22Z20161596-5996https://hdl.handle.net/10495/3099510.4314/tjpr.v15i5.111596-9827https://www.ajol.info/index.php/tjpr/article/view/136341ABSTRACT: Purpose: To evaluate the leishmanicidal and cytotoxic activity of alcohol and non-alcohol extracts and saponins from Ilex laurina. Methods: Extracts were obtained by percolation with solvents of different polarities: hexane, dichloromethane, ethyl acetate and ethanol. The ethyl acetate extract was subjected to silica gel column chromatography eluting with a step gradient of dichloromethane-methanol. All products were evaluated in vitro for leishmanicidal activity against amastigotes of leishmania panamensis and cytotoxicity on U937 cells. Results: Two saponins were isolated from the ethyl acetate extract. The ethyl acetate extract showed high leishmanicidal activity against intracellular amastigotes of L. panamensis (EC50, 7.5 ± 1.5 µg/mL) and low activity against axenic amastigotes (EC50, 52.8 ±1.6 µg/mL); this extract showed also high cytotoxicity (LC50, 57.7 ± 12.1 µg/mL). Saponin 2 exhibited high activity against intracellular amastigotes (EC50, 5.9 ± 0.5 µg/mL) but also showed high cytotoxicity on U-937 cells (EC50, 25.7 ± 6.1 µg/mL). This compound showed similar leishmanicidal activity and cytotoxicity to meglumine antimoniate and amphotericin B, respectively, drugs currently used for the treatment of leishmaniasis. Conclusions: Based on these results, Ilex laurina is a potential source of compounds that can lead to the development of new therapeutic alternatives against leishmaniasis.COL0015329COL0015099COL00082277application/pdfengUniversity of Benin, Faculty of PharmacyGrupo de Estudios BotánicosPrograma de Estudio y Control de Enfermedades Tropicales (PECET)Química de Plantas ColombianasCiudad de Benín, Nigeriainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_2df8fbb1https://purl.org/redcol/resource_type/ARTArtículo de investigaciónhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/2.5/co/http://purl.org/coar/access_right/c_abf2https://creativecommons.org/licenses/by/4.0/Leishmanicidal and cytotoxic activity of extracts and saponins from Ilex laurina (Aquifoliaceae)Actividad leishmanicida y citotóxica de extractos y saponinas de Ilex laurina (Aquifoliaceae)LeishmaniosisAntiprotozoariosAntiprotozoal AgentsSaponinasSaponinsAquifoliáceasAquifoliaceaellex laurinaTrop. J. Pharm. ResTropical Journal of Pharmaceutical973979155CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8927https://bibliotecadigital.udea.edu.co/bitstream/10495/30995/2/license_rdf1646d1f6b96dbbbc38035efc9239ac9cMD52ORIGINALCardonaWilson_2016_LeishmanicidalCytotoxicActivityOfExtracts.pdfCardonaWilson_2016_LeishmanicidalCytotoxicActivityOfExtracts.pdfArtículo de investigaciónapplication/pdf208736https://bibliotecadigital.udea.edu.co/bitstream/10495/30995/1/CardonaWilson_2016_LeishmanicidalCytotoxicActivityOfExtracts.pdfe90e7f8a0d98dfe0237128304c5197ecMD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://bibliotecadigital.udea.edu.co/bitstream/10495/30995/3/license.txt8a4605be74aa9ea9d79846c1fba20a33MD5310495/30995oai:bibliotecadigital.udea.edu.co:10495/309952022-09-30 12:58:23.387Repositorio Institucional Universidad de Antioquiaandres.perez@udea.edu.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