Leishmanicidal and cytotoxic activity of extracts and saponins from Ilex laurina (Aquifoliaceae)
ABSTRACT: Purpose: To evaluate the leishmanicidal and cytotoxic activity of alcohol and non-alcohol extracts and saponins from Ilex laurina. Methods: Extracts were obtained by percolation with solvents of different polarities: hexane, dichloromethane, ethyl acetate and ethanol. The ethyl acetate ext...
- Autores:
-
Cardona Galeano, Wilson
Pérez Cardona, Juan Manuel
Robledo Restrepo, Sara María
Alzate Guarín, Fernando
Muñoz Herrera, Diana Lorena
Herrera Ramírez, Angie
- Tipo de recurso:
- Article of investigation
- Fecha de publicación:
- 2016
- Institución:
- Universidad de Antioquia
- Repositorio:
- Repositorio UdeA
- Idioma:
- eng
- OAI Identifier:
- oai:bibliotecadigital.udea.edu.co:10495/30995
- Acceso en línea:
- https://hdl.handle.net/10495/30995
https://www.ajol.info/index.php/tjpr/article/view/136341
- Palabra clave:
- Leishmaniosis
Antiprotozoarios
Antiprotozoal Agents
Saponinas
Saponins
Aquifoliáceas
Aquifoliaceae
llex laurina
- Rights
- openAccess
- License
- http://creativecommons.org/licenses/by/2.5/co/
Summary: | ABSTRACT: Purpose: To evaluate the leishmanicidal and cytotoxic activity of alcohol and non-alcohol extracts and saponins from Ilex laurina. Methods: Extracts were obtained by percolation with solvents of different polarities: hexane, dichloromethane, ethyl acetate and ethanol. The ethyl acetate extract was subjected to silica gel column chromatography eluting with a step gradient of dichloromethane-methanol. All products were evaluated in vitro for leishmanicidal activity against amastigotes of leishmania panamensis and cytotoxicity on U937 cells. Results: Two saponins were isolated from the ethyl acetate extract. The ethyl acetate extract showed high leishmanicidal activity against intracellular amastigotes of L. panamensis (EC50, 7.5 ± 1.5 µg/mL) and low activity against axenic amastigotes (EC50, 52.8 ±1.6 µg/mL); this extract showed also high cytotoxicity (LC50, 57.7 ± 12.1 µg/mL). Saponin 2 exhibited high activity against intracellular amastigotes (EC50, 5.9 ± 0.5 µg/mL) but also showed high cytotoxicity on U-937 cells (EC50, 25.7 ± 6.1 µg/mL). This compound showed similar leishmanicidal activity and cytotoxicity to meglumine antimoniate and amphotericin B, respectively, drugs currently used for the treatment of leishmaniasis. Conclusions: Based on these results, Ilex laurina is a potential source of compounds that can lead to the development of new therapeutic alternatives against leishmaniasis. |
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