Characterization of the venom of C. d. cumanesis of Colombia : Proteomic analysis and antivenomic study
ABSTRACT: The Colombian rattlesnake Crotalus durissus cumanensis is distributed in three geographic zones of the country: the Atlantic Coast, the upper valley of the Magdalena River, and the eastern plains of the Colombian Orinoquía. Its venom induces neurological symptoms, such as eyelid ptosis, my...
- Autores:
-
Quintana Castillo, Juan Carlos
Vargas Muñoz, Leidy Johana
Segura Latorre, Cesar
Estrada Gómez, Sebastian
Bueno Sánchez, Julio César
Alarcón Pérez, Juan Carlos
- Tipo de recurso:
- Article of investigation
- Fecha de publicación:
- 2018
- Institución:
- Universidad de Antioquia
- Repositorio:
- Repositorio UdeA
- Idioma:
- eng
- OAI Identifier:
- oai:bibliotecadigital.udea.edu.co:10495/23931
- Acceso en línea:
- http://hdl.handle.net/10495/23931
- Palabra clave:
- Venenos de Serpiente
Snake Venoms
Péptidos
Peptides
C. d. cumanensis venom
HPLC-nESI-MS/MS
- Rights
- openAccess
- License
- http://creativecommons.org/licenses/by/2.5/co/
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| dc.title.spa.fl_str_mv |
Characterization of the venom of C. d. cumanesis of Colombia : Proteomic analysis and antivenomic study |
| title |
Characterization of the venom of C. d. cumanesis of Colombia : Proteomic analysis and antivenomic study |
| spellingShingle |
Characterization of the venom of C. d. cumanesis of Colombia : Proteomic analysis and antivenomic study Venenos de Serpiente Snake Venoms Péptidos Peptides C. d. cumanensis venom HPLC-nESI-MS/MS |
| title_short |
Characterization of the venom of C. d. cumanesis of Colombia : Proteomic analysis and antivenomic study |
| title_full |
Characterization of the venom of C. d. cumanesis of Colombia : Proteomic analysis and antivenomic study |
| title_fullStr |
Characterization of the venom of C. d. cumanesis of Colombia : Proteomic analysis and antivenomic study |
| title_full_unstemmed |
Characterization of the venom of C. d. cumanesis of Colombia : Proteomic analysis and antivenomic study |
| title_sort |
Characterization of the venom of C. d. cumanesis of Colombia : Proteomic analysis and antivenomic study |
| dc.creator.fl_str_mv |
Quintana Castillo, Juan Carlos Vargas Muñoz, Leidy Johana Segura Latorre, Cesar Estrada Gómez, Sebastian Bueno Sánchez, Julio César Alarcón Pérez, Juan Carlos |
| dc.contributor.author.none.fl_str_mv |
Quintana Castillo, Juan Carlos Vargas Muñoz, Leidy Johana Segura Latorre, Cesar Estrada Gómez, Sebastian Bueno Sánchez, Julio César Alarcón Pérez, Juan Carlos |
| dc.subject.decs.none.fl_str_mv |
Venenos de Serpiente Snake Venoms Péptidos Peptides |
| topic |
Venenos de Serpiente Snake Venoms Péptidos Peptides C. d. cumanensis venom HPLC-nESI-MS/MS |
| dc.subject.proposal.spa.fl_str_mv |
C. d. cumanensis venom HPLC-nESI-MS/MS |
| description |
ABSTRACT: The Colombian rattlesnake Crotalus durissus cumanensis is distributed in three geographic zones of the country: the Atlantic Coast, the upper valley of the Magdalena River, and the eastern plains of the Colombian Orinoquía. Its venom induces neurological symptoms, such as eyelid ptosis, myasthenic facies, and paralysis of the respiratory muscles, which can lead to death. Identification and analysis of C. d. cumanensis showed nine groups of proteins responsible for the neurotoxic effect, of which the crotoxin complex was the most abundant (64.71%). Immunorecognition tests of C. d. cumanensis showed that the use of a commercial antivenom manufactured in Mexico resulted in immunoreactivity. |
| publishDate |
2018 |
| dc.date.issued.none.fl_str_mv |
2018 |
| dc.date.accessioned.none.fl_str_mv |
2021-11-11T16:04:35Z |
| dc.date.available.none.fl_str_mv |
2021-11-11T16:04:35Z |
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info:eu-repo/semantics/article |
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http://purl.org/coar/version/c_970fb48d4fbd8a85 |
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info:eu-repo/semantics/publishedVersion |
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http://purl.org/coar/resource_type/c_2df8fbb1 |
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https://purl.org/redcol/resource_type/ART |
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Artículo de investigación |
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http://purl.org/coar/resource_type/c_2df8fbb1 |
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publishedVersion |
| dc.identifier.uri.none.fl_str_mv |
http://hdl.handle.net/10495/23931 |
| dc.identifier.doi.none.fl_str_mv |
10.3390/toxins10020085 |
| dc.identifier.eissn.none.fl_str_mv |
2072-6651 |
| url |
http://hdl.handle.net/10495/23931 |
| identifier_str_mv |
10.3390/toxins10020085 2072-6651 |
| dc.language.iso.spa.fl_str_mv |
eng |
| language |
eng |
| dc.relation.ispartofjournalabbrev.spa.fl_str_mv |
Toxins |
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info:eu-repo/semantics/openAccess |
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http://creativecommons.org/licenses/by/2.5/co/ |
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http://purl.org/coar/access_right/c_abf2 |
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https://creativecommons.org/licenses/by/4.0/ |
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openAccess |
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http://creativecommons.org/licenses/by/2.5/co/ http://purl.org/coar/access_right/c_abf2 https://creativecommons.org/licenses/by/4.0/ |
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12 |
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application/pdf |
| dc.publisher.spa.fl_str_mv |
MDPI |
| dc.publisher.group.spa.fl_str_mv |
Grupo Malaria Grupo Reproducción Toxinología Alternativas Terapéuticas y Alimentarias |
| dc.publisher.place.spa.fl_str_mv |
Basilea, Suiza |
| institution |
Universidad de Antioquia |
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Quintana Castillo, Juan CarlosVargas Muñoz, Leidy JohanaSegura Latorre, CesarEstrada Gómez, SebastianBueno Sánchez, Julio CésarAlarcón Pérez, Juan Carlos2021-11-11T16:04:35Z2021-11-11T16:04:35Z2018http://hdl.handle.net/10495/2393110.3390/toxins100200852072-6651ABSTRACT: The Colombian rattlesnake Crotalus durissus cumanensis is distributed in three geographic zones of the country: the Atlantic Coast, the upper valley of the Magdalena River, and the eastern plains of the Colombian Orinoquía. Its venom induces neurological symptoms, such as eyelid ptosis, myasthenic facies, and paralysis of the respiratory muscles, which can lead to death. Identification and analysis of C. d. cumanensis showed nine groups of proteins responsible for the neurotoxic effect, of which the crotoxin complex was the most abundant (64.71%). Immunorecognition tests of C. d. cumanensis showed that the use of a commercial antivenom manufactured in Mexico resulted in immunoreactivity.COL0007631COL0007524COL001447612application/pdfengMDPIGrupo MalariaGrupo ReproducciónToxinología Alternativas Terapéuticas y AlimentariasBasilea, Suizainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_2df8fbb1https://purl.org/redcol/resource_type/ARTArtículo de investigaciónhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/2.5/co/http://purl.org/coar/access_right/c_abf2https://creativecommons.org/licenses/by/4.0/Characterization of the venom of C. d. cumanesis of Colombia : Proteomic analysis and antivenomic studyVenenos de SerpienteSnake VenomsPéptidosPeptidesC. d. cumanensis venomHPLC-nESI-MS/MSToxinsToxins112102CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8927http://bibliotecadigital.udea.edu.co/bitstream/10495/23931/2/license_rdf1646d1f6b96dbbbc38035efc9239ac9cMD52LICENSElicense.txtlicense.txttext/plain; charset=utf-81748http://bibliotecadigital.udea.edu.co/bitstream/10495/23931/3/license.txt8a4605be74aa9ea9d79846c1fba20a33MD53ORIGINALQuintanaJuan_2018_CharacterizationVenomCumanesisColombia.pdfQuintanaJuan_2018_CharacterizationVenomCumanesisColombia.pdfArtículo de investigaciónapplication/pdf1975082http://bibliotecadigital.udea.edu.co/bitstream/10495/23931/1/QuintanaJuan_2018_CharacterizationVenomCumanesisColombia.pdfebba4c9883f9a7bce4fa42a2ed68a40bMD5110495/23931oai:bibliotecadigital.udea.edu.co:10495/239312021-11-11 11:05:29.134Repositorio Institucional Universidad de Antioquiaandres.perez@udea.edu.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 |