Streptomyces clavuligerus strain selection for clavulanic acid biosynthesis : a study based on culture composition effects and statistical analysis
ABSTRACT: The clinical and industrial importance of clavulanic acid (CA) promotes the search for novel strategies to boost productivity at lower costs. In this contribution, the production of CA was evaluated in two reference strains of Streptomyces clavuligerus (Sc), ATCC27064 and DSM41826; the str...
- Autores:
-
Pinilla Mendoza, Laura Inés
Toro Navarro, León Felipe
Avigone Rossa, Claudio
Peñuela Vásquez, Mariana
Ríos Estepa, Rigoberto
- Tipo de recurso:
- Article of investigation
- Fecha de publicación:
- 2018
- Institución:
- Universidad de Antioquia
- Repositorio:
- Repositorio UdeA
- Idioma:
- eng
- OAI Identifier:
- oai:bibliotecadigital.udea.edu.co:10495/23214
- Acceso en línea:
- http://hdl.handle.net/10495/23214
- Palabra clave:
- Clavulanic acid
Culture media design
Strain selection
Streptomyces clavuligerus
Antibiotics
- Rights
- openAccess
- License
- Atribución-NoComercial-SinDerivadas 2.5 Colombia
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| dc.title.spa.fl_str_mv |
Streptomyces clavuligerus strain selection for clavulanic acid biosynthesis : a study based on culture composition effects and statistical analysis |
| dc.title.alternative.spa.fl_str_mv |
Selección de una cepa de Streptomyces clavuligerus para la biosíntesis de ácido clavulánico : un estudio basado en los efectos de la composición del medio de cultivo y análisis estadístico |
| title |
Streptomyces clavuligerus strain selection for clavulanic acid biosynthesis : a study based on culture composition effects and statistical analysis |
| spellingShingle |
Streptomyces clavuligerus strain selection for clavulanic acid biosynthesis : a study based on culture composition effects and statistical analysis Clavulanic acid Culture media design Strain selection Streptomyces clavuligerus Antibiotics |
| title_short |
Streptomyces clavuligerus strain selection for clavulanic acid biosynthesis : a study based on culture composition effects and statistical analysis |
| title_full |
Streptomyces clavuligerus strain selection for clavulanic acid biosynthesis : a study based on culture composition effects and statistical analysis |
| title_fullStr |
Streptomyces clavuligerus strain selection for clavulanic acid biosynthesis : a study based on culture composition effects and statistical analysis |
| title_full_unstemmed |
Streptomyces clavuligerus strain selection for clavulanic acid biosynthesis : a study based on culture composition effects and statistical analysis |
| title_sort |
Streptomyces clavuligerus strain selection for clavulanic acid biosynthesis : a study based on culture composition effects and statistical analysis |
| dc.creator.fl_str_mv |
Pinilla Mendoza, Laura Inés Toro Navarro, León Felipe Avigone Rossa, Claudio Peñuela Vásquez, Mariana Ríos Estepa, Rigoberto |
| dc.contributor.author.none.fl_str_mv |
Pinilla Mendoza, Laura Inés Toro Navarro, León Felipe Avigone Rossa, Claudio Peñuela Vásquez, Mariana Ríos Estepa, Rigoberto |
| dc.subject.proposal.spa.fl_str_mv |
Clavulanic acid Culture media design Strain selection Streptomyces clavuligerus Antibiotics |
| topic |
Clavulanic acid Culture media design Strain selection Streptomyces clavuligerus Antibiotics |
| description |
ABSTRACT: The clinical and industrial importance of clavulanic acid (CA) promotes the search for novel strategies to boost productivity at lower costs. In this contribution, the production of CA was evaluated in two reference strains of Streptomyces clavuligerus (Sc), ATCC27064 and DSM41826; the strains were tested in different culture media, using product yields and culture media costs as selection criteria. On average, ScATCC reached a concentration of CA 1.6-fold higher than ScDSM, using the isolated soybean protein (ISP) culture medium. A central composite experimental design was used to optimize the medium, by studying the influence of ISP and K2HPO4 concentration on the production of CA. The maximum CA concentration obtained in this optimized medium was 56 mg·L-1, close to the theoretical optimum. Our results confirm that a culture medium containing a rich carbon source such as glycerol and/or starch provides the best conditions for CA production. |
| publishDate |
2018 |
| dc.date.issued.none.fl_str_mv |
2018 |
| dc.date.accessioned.none.fl_str_mv |
2021-10-19T02:40:50Z |
| dc.date.available.none.fl_str_mv |
2021-10-19T02:40:50Z |
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info:eu-repo/semantics/article |
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http://purl.org/coar/version/c_970fb48d4fbd8a85 |
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info:eu-repo/semantics/publishedVersion |
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http://purl.org/coar/resource_type/c_2df8fbb1 |
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https://purl.org/redcol/resource_type/ART |
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Artículo de investigación |
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http://purl.org/coar/resource_type/c_2df8fbb1 |
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publishedVersion |
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0012-7353 |
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http://hdl.handle.net/10495/23214 |
| dc.identifier.doi.none.fl_str_mv |
10.15446/dyna.v85n205.69560 |
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2346-2183 |
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0012-7353 10.15446/dyna.v85n205.69560 2346-2183 |
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http://hdl.handle.net/10495/23214 |
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eng |
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eng |
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Dyna |
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info:eu-repo/semantics/openAccess |
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Atribución-NoComercial-SinDerivadas 2.5 Colombia |
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http://creativecommons.org/licenses/by-nc-nd/2.5/co/ |
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http://purl.org/coar/access_right/c_abf2 |
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https://creativecommons.org/licenses/by-nc-nd/4.0/ |
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openAccess |
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Atribución-NoComercial-SinDerivadas 2.5 Colombia http://creativecommons.org/licenses/by-nc-nd/2.5/co/ http://purl.org/coar/access_right/c_abf2 https://creativecommons.org/licenses/by-nc-nd/4.0/ |
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Universidad Nacional de Colombia, Facultad de Minas, Centro de Publicaciones |
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Bioprocesos |
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Medellín, Colombia |
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Universidad de Antioquia |
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http://bibliotecadigital.udea.edu.co/bitstream/10495/23214/1/PinillaLaura_2018_StrptomycesClavuligerusStrain.pdf http://bibliotecadigital.udea.edu.co/bitstream/10495/23214/2/license_rdf http://bibliotecadigital.udea.edu.co/bitstream/10495/23214/3/license.txt |
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andres.perez@udea.edu.co |
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1812173208226365440 |
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Pinilla Mendoza, Laura InésToro Navarro, León FelipeAvigone Rossa, ClaudioPeñuela Vásquez, MarianaRíos Estepa, Rigoberto2021-10-19T02:40:50Z2021-10-19T02:40:50Z20180012-7353http://hdl.handle.net/10495/2321410.15446/dyna.v85n205.695602346-2183ABSTRACT: The clinical and industrial importance of clavulanic acid (CA) promotes the search for novel strategies to boost productivity at lower costs. In this contribution, the production of CA was evaluated in two reference strains of Streptomyces clavuligerus (Sc), ATCC27064 and DSM41826; the strains were tested in different culture media, using product yields and culture media costs as selection criteria. On average, ScATCC reached a concentration of CA 1.6-fold higher than ScDSM, using the isolated soybean protein (ISP) culture medium. A central composite experimental design was used to optimize the medium, by studying the influence of ISP and K2HPO4 concentration on the production of CA. The maximum CA concentration obtained in this optimized medium was 56 mg·L-1, close to the theoretical optimum. Our results confirm that a culture medium containing a rich carbon source such as glycerol and/or starch provides the best conditions for CA production.RESUMEN: La importancia clínica e industrial del ácido clavulánico (AC) promueve la búsqueda de nuevas estrategias para aumentar su productividad a un menor costo. En esta contribución, se evaluó la producción de AC en dos cepas de referencia de Streptomyces clavuligerus (Sc), ATCC27064 y DSM41826; éstas se probaron en diferentes medios de cultivo; los rendimientos de producto y costo de los medios se usaron como criterios de selección. En promedio, ScATCC alcanzó una concentración de AC 1.6-veces mayor que ScDSM, en el medio de proteína de soya (ISP). Se optimizó el medio con un diseño experimental central compuesto, estudiando la influencia del ISP y K2HPO4 sobre la producción de AC. La concentración máxima de AC obtenida en el medio optimizado (56mg·L-1 ) estuvo cerca del óptimo teórico. Nuestros resultados confirman que medios de cultivo con fuentes de carbono como glicerol y/o almidón proporcionan las mejores condiciones para la producción de AC.COL00237158application/pdfengUniversidad Nacional de Colombia, Facultad de Minas, Centro de PublicacionesBioprocesosMedellín, Colombiainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_2df8fbb1https://purl.org/redcol/resource_type/ARTArtículo de investigaciónhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/openAccessAtribución-NoComercial-SinDerivadas 2.5 Colombiahttp://creativecommons.org/licenses/by-nc-nd/2.5/co/http://purl.org/coar/access_right/c_abf2https://creativecommons.org/licenses/by-nc-nd/4.0/Streptomyces clavuligerus strain selection for clavulanic acid biosynthesis : a study based on culture composition effects and statistical analysisSelección de una cepa de Streptomyces clavuligerus para la biosíntesis de ácido clavulánico : un estudio basado en los efectos de la composición del medio de cultivo y análisis estadísticoClavulanic acidCulture media designStrain selectionStreptomyces clavuligerusAntibioticsDynaDyna11111885205ORIGINALPinillaLaura_2018_StrptomycesClavuligerusStrain.pdfPinillaLaura_2018_StrptomycesClavuligerusStrain.pdfArtículo de investigaciónapplication/pdf607376http://bibliotecadigital.udea.edu.co/bitstream/10495/23214/1/PinillaLaura_2018_StrptomycesClavuligerusStrain.pdf3f21bb2dcdb2ac8bae3c5146b84a928bMD51CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8823http://bibliotecadigital.udea.edu.co/bitstream/10495/23214/2/license_rdfb88b088d9957e670ce3b3fbe2eedbc13MD52LICENSElicense.txtlicense.txttext/plain; charset=utf-81748http://bibliotecadigital.udea.edu.co/bitstream/10495/23214/3/license.txt8a4605be74aa9ea9d79846c1fba20a33MD5310495/23214oai:bibliotecadigital.udea.edu.co:10495/232142021-10-18 21:40:50.615Repositorio Institucional Universidad de Antioquiaandres.perez@udea.edu.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 |