Nanoparticles specific for Slan+ monocytes as potential therapeutic tools in systemic lupus erythematosus
ABSTRACT: Background and aims 6-sulfoLacNAc+ monocytes (slanMos) are a subset of nonclassical monocytes, which are considered pro-inflammatory cells in different diseases, including the autoimmune ones, such as systemic lupus erythematosus (SLE). Reduction and dysfunction of circulating nonclassical...
- Autores:
-
Álvarez Díaz, Karen Dayanna
Losada Vanegas, Paula Ximena
Pineda Tamayo, Ricardo Antonio
Giraldo Morales, Luis Fernando
Vásquez Duque, Gloria María
Mauricio, Rojas López
Ruiz Agudelo, Esneyder
- Tipo de recurso:
- http://purl.org/coar/resource_type/c_5794
- Fecha de publicación:
- 2022
- Institución:
- Universidad de Antioquia
- Repositorio:
- Repositorio UdeA
- Idioma:
- eng
- OAI Identifier:
- oai:bibliotecadigital.udea.edu.co:10495/29564
- Acceso en línea:
- http://hdl.handle.net/10495/29564
- Palabra clave:
- Autoinmunidad
Autoimmunity
Monocitos
Monocytes
Inflamación
Inflammation
- Rights
- openAccess
- License
- Atribución-NoComercial-CompartirIgual 2.5 Colombia
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UDEA2 |
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Repositorio UdeA |
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|
dc.title.spa.fl_str_mv |
Nanoparticles specific for Slan+ monocytes as potential therapeutic tools in systemic lupus erythematosus |
title |
Nanoparticles specific for Slan+ monocytes as potential therapeutic tools in systemic lupus erythematosus |
spellingShingle |
Nanoparticles specific for Slan+ monocytes as potential therapeutic tools in systemic lupus erythematosus Autoinmunidad Autoimmunity Monocitos Monocytes Inflamación Inflammation |
title_short |
Nanoparticles specific for Slan+ monocytes as potential therapeutic tools in systemic lupus erythematosus |
title_full |
Nanoparticles specific for Slan+ monocytes as potential therapeutic tools in systemic lupus erythematosus |
title_fullStr |
Nanoparticles specific for Slan+ monocytes as potential therapeutic tools in systemic lupus erythematosus |
title_full_unstemmed |
Nanoparticles specific for Slan+ monocytes as potential therapeutic tools in systemic lupus erythematosus |
title_sort |
Nanoparticles specific for Slan+ monocytes as potential therapeutic tools in systemic lupus erythematosus |
dc.creator.fl_str_mv |
Álvarez Díaz, Karen Dayanna Losada Vanegas, Paula Ximena Pineda Tamayo, Ricardo Antonio Giraldo Morales, Luis Fernando Vásquez Duque, Gloria María Mauricio, Rojas López Ruiz Agudelo, Esneyder |
dc.contributor.author.none.fl_str_mv |
Álvarez Díaz, Karen Dayanna Losada Vanegas, Paula Ximena Pineda Tamayo, Ricardo Antonio Giraldo Morales, Luis Fernando Vásquez Duque, Gloria María Mauricio, Rojas López Ruiz Agudelo, Esneyder |
dc.contributor.conferencename.spa.fl_str_mv |
International Congress on Autoimmunity (13 : 11 de Junio 2022 : Atenas, Grecia) |
dc.subject.decs.none.fl_str_mv |
Autoinmunidad Autoimmunity Monocitos Monocytes Inflamación Inflammation |
topic |
Autoinmunidad Autoimmunity Monocitos Monocytes Inflamación Inflammation |
description |
ABSTRACT: Background and aims 6-sulfoLacNAc+ monocytes (slanMos) are a subset of nonclassical monocytes, which are considered pro-inflammatory cells in different diseases, including the autoimmune ones, such as systemic lupus erythematosus (SLE). Reduction and dysfunction of circulating nonclassical monocytes and recruitment of these cells to inflammation sites in SLE patients have been previously reported by our Group. We proposed encapsulating Itacitinib into nanoparticles to inhibit the JAK-STAT pathway in SlanMo from SLE patients and other chronic inflammatory diseases. Methods Monocyte subpopulations were evaluated in SLE patients (n=10, from ARTMEDICA) and healthy controls (n=10) by flow cytometry markers (CD14, CD16, HLA-DR, and Slan). SlanMos were isolated using a commercial kit. Inhibition of the JAK-STAT pathway was evaluated with Baricitinib or Itacitinib and stimulated with IFN-ɤ. HLA-DR, CD64, and CD69, p-STAT-1, and TNF-α accumulation were determined by flow cytometry. We evaluated the specific binding of Poly-lactic-co-glycolic acid (PLGA), galactosamine-PLGA, and wheat germ agglutinin (WGA)-PLGA nanoparticles by leukocytes. Results Patients had a significantly lower number of circulating SlanMo. Itactinib had the highest inhibitory effect on the JAK-STAT pathway. Then, we will design an Itacitinib encapsulated nanoparticle to target the SlanMo. We had established that PLGA-WGA nanoparticles were uptook efficiently by monocytes compared with other leukocytes. Suggesting the potential role of these nanoparticles to target monocytes and possibly more specifically SlanMos for the treatment of SLE. Conclusions The uptake of PLGA-WGA nanoparticles by monocytes suggests the potential role of these nanoparticles to target these cells and possibly more specifically SlanMo for the treatment of SLE. |
publishDate |
2022 |
dc.date.accessioned.none.fl_str_mv |
2022-07-06T16:48:18Z |
dc.date.available.none.fl_str_mv |
2022-07-06T16:48:18Z |
dc.date.issued.none.fl_str_mv |
2022 |
dc.type.spa.fl_str_mv |
info:eu-repo/semantics/conferenceObject |
dc.type.coar.fl_str_mv |
http://purl.org/coar/resource_type/c_c94f |
dc.type.coarversion.fl_str_mv |
http://purl.org/coar/version/c_b1a7d7d4d402bcce |
dc.type.hasversion.spa.fl_str_mv |
info:eu-repo/semantics/draft |
dc.type.coar.spa.fl_str_mv |
http://purl.org/coar/resource_type/c_5794 |
dc.type.redcol.spa.fl_str_mv |
https://purl.org/redcol/resource_type/EC |
dc.type.local.spa.fl_str_mv |
Documento de conferencia |
format |
http://purl.org/coar/resource_type/c_5794 |
status_str |
draft |
dc.identifier.uri.none.fl_str_mv |
http://hdl.handle.net/10495/29564 |
url |
http://hdl.handle.net/10495/29564 |
dc.language.iso.spa.fl_str_mv |
eng |
language |
eng |
dc.rights.spa.fl_str_mv |
info:eu-repo/semantics/openAccess |
dc.rights.*.fl_str_mv |
Atribución-NoComercial-CompartirIgual 2.5 Colombia |
dc.rights.uri.*.fl_str_mv |
http://creativecommons.org/licenses/by-nc-sa/2.5/co/ |
dc.rights.accessrights.spa.fl_str_mv |
http://purl.org/coar/access_right/c_abf2 |
dc.rights.creativecommons.spa.fl_str_mv |
https://creativecommons.org/licenses/by-nc-sa/4.0/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
Atribución-NoComercial-CompartirIgual 2.5 Colombia http://creativecommons.org/licenses/by-nc-sa/2.5/co/ http://purl.org/coar/access_right/c_abf2 https://creativecommons.org/licenses/by-nc-sa/4.0/ |
dc.format.mimetype.spa.fl_str_mv |
application/pdf |
dc.publisher.group.spa.fl_str_mv |
Grupo de Inmunología Celular e Inmunogenética |
institution |
Universidad de Antioquia |
bitstream.url.fl_str_mv |
https://bibliotecadigital.udea.edu.co/bitstream/10495/29564/1/AlvarezKaren_2022_Nanoparticles-Specific-Slan.pptx https://bibliotecadigital.udea.edu.co/bitstream/10495/29564/2/AlvarezKaren_2022_Nanoparticles-Specific-Slan.pdf https://bibliotecadigital.udea.edu.co/bitstream/10495/29564/3/license_rdf https://bibliotecadigital.udea.edu.co/bitstream/10495/29564/4/license.txt |
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64651c912d4d3f2bdc716049d4faf5b3 9414dd7308417c3116b20c9d2eb16199 e2060682c9c70d4d30c83c51448f4eed 8a4605be74aa9ea9d79846c1fba20a33 |
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MD5 MD5 MD5 MD5 |
repository.name.fl_str_mv |
Repositorio Institucional Universidad de Antioquia |
repository.mail.fl_str_mv |
andres.perez@udea.edu.co |
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1812173203653525504 |
spelling |
Álvarez Díaz, Karen DayannaLosada Vanegas, Paula XimenaPineda Tamayo, Ricardo AntonioGiraldo Morales, Luis FernandoVásquez Duque, Gloria MaríaMauricio, Rojas LópezRuiz Agudelo, EsneyderInternational Congress on Autoimmunity (13 : 11 de Junio 2022 : Atenas, Grecia)2022-07-06T16:48:18Z2022-07-06T16:48:18Z2022http://hdl.handle.net/10495/29564ABSTRACT: Background and aims 6-sulfoLacNAc+ monocytes (slanMos) are a subset of nonclassical monocytes, which are considered pro-inflammatory cells in different diseases, including the autoimmune ones, such as systemic lupus erythematosus (SLE). Reduction and dysfunction of circulating nonclassical monocytes and recruitment of these cells to inflammation sites in SLE patients have been previously reported by our Group. We proposed encapsulating Itacitinib into nanoparticles to inhibit the JAK-STAT pathway in SlanMo from SLE patients and other chronic inflammatory diseases. Methods Monocyte subpopulations were evaluated in SLE patients (n=10, from ARTMEDICA) and healthy controls (n=10) by flow cytometry markers (CD14, CD16, HLA-DR, and Slan). SlanMos were isolated using a commercial kit. Inhibition of the JAK-STAT pathway was evaluated with Baricitinib or Itacitinib and stimulated with IFN-ɤ. HLA-DR, CD64, and CD69, p-STAT-1, and TNF-α accumulation were determined by flow cytometry. We evaluated the specific binding of Poly-lactic-co-glycolic acid (PLGA), galactosamine-PLGA, and wheat germ agglutinin (WGA)-PLGA nanoparticles by leukocytes. Results Patients had a significantly lower number of circulating SlanMo. Itactinib had the highest inhibitory effect on the JAK-STAT pathway. Then, we will design an Itacitinib encapsulated nanoparticle to target the SlanMo. We had established that PLGA-WGA nanoparticles were uptook efficiently by monocytes compared with other leukocytes. Suggesting the potential role of these nanoparticles to target monocytes and possibly more specifically SlanMos for the treatment of SLE. Conclusions The uptake of PLGA-WGA nanoparticles by monocytes suggests the potential role of these nanoparticles to target these cells and possibly more specifically SlanMo for the treatment of SLE.COL0008639application/pdfenginfo:eu-repo/semantics/draftinfo:eu-repo/semantics/conferenceObjecthttp://purl.org/coar/resource_type/c_5794http://purl.org/coar/resource_type/c_c94fhttps://purl.org/redcol/resource_type/ECDocumento de conferenciahttp://purl.org/coar/version/c_b1a7d7d4d402bcceinfo:eu-repo/semantics/openAccessAtribución-NoComercial-CompartirIgual 2.5 Colombiahttp://creativecommons.org/licenses/by-nc-sa/2.5/co/http://purl.org/coar/access_right/c_abf2https://creativecommons.org/licenses/by-nc-sa/4.0/Nanoparticles specific for Slan+ monocytes as potential therapeutic tools in systemic lupus erythematosusGrupo de Inmunología Celular e InmunogenéticaAutoinmunidadAutoimmunityMonocitosMonocytesInflamaciónInflammation13th International Congress on AutoimmunityAtenas, Grecia2022-06-10/2022-06-13ORIGINALAlvarezKaren_2022_Nanoparticles-Specific-Slan.pptxAlvarezKaren_2022_Nanoparticles-Specific-Slan.pptxPresentaciónapplication/vnd.openxmlformats-officedocument.presentationml.presentation1885017https://bibliotecadigital.udea.edu.co/bitstream/10495/29564/1/AlvarezKaren_2022_Nanoparticles-Specific-Slan.pptx64651c912d4d3f2bdc716049d4faf5b3MD51AlvarezKaren_2022_Nanoparticles-Specific-Slan.pdfAlvarezKaren_2022_Nanoparticles-Specific-Slan.pdfResumenapplication/pdf104396https://bibliotecadigital.udea.edu.co/bitstream/10495/29564/2/AlvarezKaren_2022_Nanoparticles-Specific-Slan.pdf9414dd7308417c3116b20c9d2eb16199MD52CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-81051https://bibliotecadigital.udea.edu.co/bitstream/10495/29564/3/license_rdfe2060682c9c70d4d30c83c51448f4eedMD53LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://bibliotecadigital.udea.edu.co/bitstream/10495/29564/4/license.txt8a4605be74aa9ea9d79846c1fba20a33MD5410495/29564oai:bibliotecadigital.udea.edu.co:10495/295642022-07-06 12:18:15.557Repositorio Institucional Universidad de Antioquiaandres.perez@udea.edu.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 |