An interferon gamma release assay specific for Histoplasma capsulatum to detect asymptomatic infected individuals: A proof of concept study
ABSTRACT : Histoplasmosis is the most common endemic mycosis in the Americas. Currently, there is no laboratory test capable to detect subclinical or latent infections by Histoplasma capsulatum (Hc), which might develop as severe infections in immunocompromised individuals. For the first time to our...
- Autores:
-
Rubio Carrasquilla, Marcela
Santa Escobar, Cristian David
Rondón Marín, Juan Pablo
Botero Garcés, Jorge Humberto
Guimaraes, Allan J.
Moreno Frias, Ernesto
Cano Restrepo, Luz Elena
- Tipo de recurso:
- Article of investigation
- Fecha de publicación:
- 2018
- Institución:
- Universidad de Antioquia
- Repositorio:
- Repositorio UdeA
- Idioma:
- eng
- OAI Identifier:
- oai:bibliotecadigital.udea.edu.co:10495/26552
- Acceso en línea:
- http://hdl.handle.net/10495/26552
- Palabra clave:
- Antígenos Fúngicos
Antigens, Fungal
Histoplasmosis
Histoplasma
Histoplasmosis
Ensayos de Liberación de Interferón gamma
Interferon-gamma Release Tests
Valor Predictivo de las Pruebas
Predictive Value of Tests
Prueba de Estudio Conceptual
Proof of Concept Study
- Rights
- openAccess
- License
- http://creativecommons.org/licenses/by-nc/2.5/co/
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|
dc.title.spa.fl_str_mv |
An interferon gamma release assay specific for Histoplasma capsulatum to detect asymptomatic infected individuals: A proof of concept study |
title |
An interferon gamma release assay specific for Histoplasma capsulatum to detect asymptomatic infected individuals: A proof of concept study |
spellingShingle |
An interferon gamma release assay specific for Histoplasma capsulatum to detect asymptomatic infected individuals: A proof of concept study Antígenos Fúngicos Antigens, Fungal Histoplasmosis Histoplasma Histoplasmosis Ensayos de Liberación de Interferón gamma Interferon-gamma Release Tests Valor Predictivo de las Pruebas Predictive Value of Tests Prueba de Estudio Conceptual Proof of Concept Study |
title_short |
An interferon gamma release assay specific for Histoplasma capsulatum to detect asymptomatic infected individuals: A proof of concept study |
title_full |
An interferon gamma release assay specific for Histoplasma capsulatum to detect asymptomatic infected individuals: A proof of concept study |
title_fullStr |
An interferon gamma release assay specific for Histoplasma capsulatum to detect asymptomatic infected individuals: A proof of concept study |
title_full_unstemmed |
An interferon gamma release assay specific for Histoplasma capsulatum to detect asymptomatic infected individuals: A proof of concept study |
title_sort |
An interferon gamma release assay specific for Histoplasma capsulatum to detect asymptomatic infected individuals: A proof of concept study |
dc.creator.fl_str_mv |
Rubio Carrasquilla, Marcela Santa Escobar, Cristian David Rondón Marín, Juan Pablo Botero Garcés, Jorge Humberto Guimaraes, Allan J. Moreno Frias, Ernesto Cano Restrepo, Luz Elena |
dc.contributor.author.none.fl_str_mv |
Rubio Carrasquilla, Marcela Santa Escobar, Cristian David Rondón Marín, Juan Pablo Botero Garcés, Jorge Humberto Guimaraes, Allan J. Moreno Frias, Ernesto Cano Restrepo, Luz Elena |
dc.subject.decs.none.fl_str_mv |
Antígenos Fúngicos Antigens, Fungal Histoplasmosis Histoplasma Histoplasmosis Ensayos de Liberación de Interferón gamma Interferon-gamma Release Tests Valor Predictivo de las Pruebas Predictive Value of Tests Prueba de Estudio Conceptual Proof of Concept Study |
topic |
Antígenos Fúngicos Antigens, Fungal Histoplasmosis Histoplasma Histoplasmosis Ensayos de Liberación de Interferón gamma Interferon-gamma Release Tests Valor Predictivo de las Pruebas Predictive Value of Tests Prueba de Estudio Conceptual Proof of Concept Study |
description |
ABSTRACT : Histoplasmosis is the most common endemic mycosis in the Americas. Currently, there is no laboratory test capable to detect subclinical or latent infections by Histoplasma capsulatum (Hc), which might develop as severe infections in immunocompromised individuals. For the first time to our knowledge, we explore the suitability of an interferon gamma release assay (IGRA) to detect latent Hc infection in asymptomatic individuals. A cohort of 126 volunteers was enrolled in the study, 13 of which underwent a Hc infection in the past, and 93 of them showing risk factors for this infection. The remaining 20 participants did not refer any risk factors of Hc infection, but eight of them showed evidences of infection with Mycobacterium tuberculosis. All participants were recruited in Medellin, Colombia, between January 2014 and December 2017. Whole blood samples were cultured with four different Hc crude antigens and phytohemaglutinin as positive control. The interferon (IFN)-γ released by T lymphocytes upon antigen stimulation was quantified by ELISA. A defined cutoff value of 20 pg/ml for the IFN-γ concentration allowed us to distinguish between the group with documented past infections and the group of noninfected individuals with high sensitivity (70– 92%) and specificity (85–95%), for the four tested antigens. Positive 82–95% and negative 77–92% predictive values were also very high, comparable to those reported for commercially available IGRAs. The new test constitutes a promising screening method to detect individuals with latent Hc infection, even decades after the primary infection, as evidenced in this study. |
publishDate |
2018 |
dc.date.issued.none.fl_str_mv |
2018 |
dc.date.accessioned.none.fl_str_mv |
2022-03-14T16:58:20Z |
dc.date.available.none.fl_str_mv |
2022-03-14T16:58:20Z |
dc.type.spa.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.hasversion.spa.fl_str_mv |
info:eu-repo/semantics/acceptedVersion |
dc.type.coar.spa.fl_str_mv |
http://purl.org/coar/resource_type/c_2df8fbb1 |
dc.type.redcol.spa.fl_str_mv |
https://purl.org/redcol/resource_type/ART |
dc.type.local.spa.fl_str_mv |
Artículo de investigación |
format |
http://purl.org/coar/resource_type/c_2df8fbb1 |
status_str |
acceptedVersion |
dc.identifier.citation.spa.fl_str_mv |
Rubio-Carrasquilla M, Santa CD, Rendón JP, Botero-Garcés J, Guimarães AJ, Moreno E, Cano LE. An interferon gamma release assay specific for Histoplasma capsulatum to detect asymptomatic infected individuals: A proof of concept study. Med Mycol. 2019 Aug 1;57(6):724-732. doi: 10.1093/mmy/myy131. Rubio-Carrasquilla M, Santa CD, Rendón JP, Botero-Garcés J, Guimarães AJ, Moreno E, Cano LE. An interferon gamma release assay specific for Histoplasma capsulatum to detect asymptomatic infected individuals: A proof of concept study. Med Mycol. 2019 Aug 1;57(6):724-732. doi: 10.1093/mmy/myy131. |
dc.identifier.issn.none.fl_str_mv |
1369-3786 |
dc.identifier.uri.none.fl_str_mv |
http://hdl.handle.net/10495/26552 |
dc.identifier.doi.none.fl_str_mv |
10.1093/mmy/myy131 |
dc.identifier.eissn.none.fl_str_mv |
1460-2709 |
identifier_str_mv |
Rubio-Carrasquilla M, Santa CD, Rendón JP, Botero-Garcés J, Guimarães AJ, Moreno E, Cano LE. An interferon gamma release assay specific for Histoplasma capsulatum to detect asymptomatic infected individuals: A proof of concept study. Med Mycol. 2019 Aug 1;57(6):724-732. doi: 10.1093/mmy/myy131. Rubio-Carrasquilla M, Santa CD, Rendón JP, Botero-Garcés J, Guimarães AJ, Moreno E, Cano LE. An interferon gamma release assay specific for Histoplasma capsulatum to detect asymptomatic infected individuals: A proof of concept study. Med Mycol. 2019 Aug 1;57(6):724-732. doi: 10.1093/mmy/myy131. 1369-3786 10.1093/mmy/myy131 1460-2709 |
url |
http://hdl.handle.net/10495/26552 |
dc.language.iso.spa.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartofjournalabbrev.spa.fl_str_mv |
Med. Mycol. |
dc.rights.spa.fl_str_mv |
info:eu-repo/semantics/openAccess |
dc.rights.uri.*.fl_str_mv |
http://creativecommons.org/licenses/by-nc/2.5/co/ |
dc.rights.accessrights.spa.fl_str_mv |
http://purl.org/coar/access_right/c_abf2 |
dc.rights.creativecommons.spa.fl_str_mv |
https://creativecommons.org/licenses/by-nc/4.0/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by-nc/2.5/co/ http://purl.org/coar/access_right/c_abf2 https://creativecommons.org/licenses/by-nc/4.0/ |
dc.format.extent.spa.fl_str_mv |
9 |
dc.format.mimetype.spa.fl_str_mv |
application/pdf |
dc.publisher.spa.fl_str_mv |
Oxford University Press |
dc.publisher.group.spa.fl_str_mv |
Grupo de Parasitología Universidad de Antioquia Micología Médica y Experimental |
dc.publisher.place.spa.fl_str_mv |
Oxford, Inglaterra |
institution |
Universidad de Antioquia |
bitstream.url.fl_str_mv |
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Repositorio Institucional Universidad de Antioquia |
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spelling |
Rubio Carrasquilla, MarcelaSanta Escobar, Cristian DavidRondón Marín, Juan PabloBotero Garcés, Jorge HumbertoGuimaraes, Allan J.Moreno Frias, ErnestoCano Restrepo, Luz Elena2022-03-14T16:58:20Z2022-03-14T16:58:20Z2018Rubio-Carrasquilla M, Santa CD, Rendón JP, Botero-Garcés J, Guimarães AJ, Moreno E, Cano LE. An interferon gamma release assay specific for Histoplasma capsulatum to detect asymptomatic infected individuals: A proof of concept study. Med Mycol. 2019 Aug 1;57(6):724-732. doi: 10.1093/mmy/myy131. Rubio-Carrasquilla M, Santa CD, Rendón JP, Botero-Garcés J, Guimarães AJ, Moreno E, Cano LE. An interferon gamma release assay specific for Histoplasma capsulatum to detect asymptomatic infected individuals: A proof of concept study. Med Mycol. 2019 Aug 1;57(6):724-732. doi: 10.1093/mmy/myy131.1369-3786http://hdl.handle.net/10495/2655210.1093/mmy/myy1311460-2709ABSTRACT : Histoplasmosis is the most common endemic mycosis in the Americas. Currently, there is no laboratory test capable to detect subclinical or latent infections by Histoplasma capsulatum (Hc), which might develop as severe infections in immunocompromised individuals. For the first time to our knowledge, we explore the suitability of an interferon gamma release assay (IGRA) to detect latent Hc infection in asymptomatic individuals. A cohort of 126 volunteers was enrolled in the study, 13 of which underwent a Hc infection in the past, and 93 of them showing risk factors for this infection. The remaining 20 participants did not refer any risk factors of Hc infection, but eight of them showed evidences of infection with Mycobacterium tuberculosis. All participants were recruited in Medellin, Colombia, between January 2014 and December 2017. Whole blood samples were cultured with four different Hc crude antigens and phytohemaglutinin as positive control. The interferon (IFN)-γ released by T lymphocytes upon antigen stimulation was quantified by ELISA. A defined cutoff value of 20 pg/ml for the IFN-γ concentration allowed us to distinguish between the group with documented past infections and the group of noninfected individuals with high sensitivity (70– 92%) and specificity (85–95%), for the four tested antigens. Positive 82–95% and negative 77–92% predictive values were also very high, comparable to those reported for commercially available IGRAs. The new test constitutes a promising screening method to detect individuals with latent Hc infection, even decades after the primary infection, as evidenced in this study.COL0013709COL00075069application/pdfengOxford University PressGrupo de Parasitología Universidad de AntioquiaMicología Médica y ExperimentalOxford, Inglaterrainfo:eu-repo/semantics/acceptedVersioninfo:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_2df8fbb1https://purl.org/redcol/resource_type/ARTArtículo de investigacióninfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc/2.5/co/http://purl.org/coar/access_right/c_abf2https://creativecommons.org/licenses/by-nc/4.0/An interferon gamma release assay specific for Histoplasma capsulatum to detect asymptomatic infected individuals: A proof of concept studyAntígenos FúngicosAntigens, FungalHistoplasmosisHistoplasmaHistoplasmosisEnsayos de Liberación de Interferón gammaInterferon-gamma Release TestsValor Predictivo de las PruebasPredictive Value of TestsPrueba de Estudio ConceptualProof of Concept StudyMed. Mycol.Medical Mycology724732576CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8933http://bibliotecadigital.udea.edu.co/bitstream/10495/26552/2/license_rdfc0c92b0ffc8b7d22d9cf56754a416a76MD52ORIGINALRubioMarcela_2018_HistoplasmaCapsulatum.pdfRubioMarcela_2018_HistoplasmaCapsulatum.pdfArtículo de investigaciónapplication/pdf1039021http://bibliotecadigital.udea.edu.co/bitstream/10495/26552/1/RubioMarcela_2018_HistoplasmaCapsulatum.pdfe01bbeb2a02658d20d30e95d0aba9fe4MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748http://bibliotecadigital.udea.edu.co/bitstream/10495/26552/3/license.txt8a4605be74aa9ea9d79846c1fba20a33MD5310495/26552oai:bibliotecadigital.udea.edu.co:10495/265522022-04-22 10:24:17.588Repositorio Institucional Universidad de Antioquiaandres.perez@udea.edu.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 |