An interferon gamma release assay specific for Histoplasma capsulatum to detect asymptomatic infected individuals: A proof of concept study

ABSTRACT : Histoplasmosis is the most common endemic mycosis in the Americas. Currently, there is no laboratory test capable to detect subclinical or latent infections by Histoplasma capsulatum (Hc), which might develop as severe infections in immunocompromised individuals. For the first time to our...

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Autores:
Rubio Carrasquilla, Marcela
Santa Escobar, Cristian David
Rondón Marín, Juan Pablo
Botero Garcés, Jorge Humberto
Guimaraes, Allan J.
Moreno Frias, Ernesto
Cano Restrepo, Luz Elena
Tipo de recurso:
Article of investigation
Fecha de publicación:
2018
Institución:
Universidad de Antioquia
Repositorio:
Repositorio UdeA
Idioma:
eng
OAI Identifier:
oai:bibliotecadigital.udea.edu.co:10495/26552
Acceso en línea:
http://hdl.handle.net/10495/26552
Palabra clave:
Antígenos Fúngicos
Antigens, Fungal
Histoplasmosis
Histoplasma
Histoplasmosis
Ensayos de Liberación de Interferón gamma
Interferon-gamma Release Tests
Valor Predictivo de las Pruebas
Predictive Value of Tests
Prueba de Estudio Conceptual
Proof of Concept Study
Rights
openAccess
License
http://creativecommons.org/licenses/by-nc/2.5/co/
id UDEA2_d76215a5eda12963a6ab6cc72e04bb8e
oai_identifier_str oai:bibliotecadigital.udea.edu.co:10495/26552
network_acronym_str UDEA2
network_name_str Repositorio UdeA
repository_id_str
dc.title.spa.fl_str_mv An interferon gamma release assay specific for Histoplasma capsulatum to detect asymptomatic infected individuals: A proof of concept study
title An interferon gamma release assay specific for Histoplasma capsulatum to detect asymptomatic infected individuals: A proof of concept study
spellingShingle An interferon gamma release assay specific for Histoplasma capsulatum to detect asymptomatic infected individuals: A proof of concept study
Antígenos Fúngicos
Antigens, Fungal
Histoplasmosis
Histoplasma
Histoplasmosis
Ensayos de Liberación de Interferón gamma
Interferon-gamma Release Tests
Valor Predictivo de las Pruebas
Predictive Value of Tests
Prueba de Estudio Conceptual
Proof of Concept Study
title_short An interferon gamma release assay specific for Histoplasma capsulatum to detect asymptomatic infected individuals: A proof of concept study
title_full An interferon gamma release assay specific for Histoplasma capsulatum to detect asymptomatic infected individuals: A proof of concept study
title_fullStr An interferon gamma release assay specific for Histoplasma capsulatum to detect asymptomatic infected individuals: A proof of concept study
title_full_unstemmed An interferon gamma release assay specific for Histoplasma capsulatum to detect asymptomatic infected individuals: A proof of concept study
title_sort An interferon gamma release assay specific for Histoplasma capsulatum to detect asymptomatic infected individuals: A proof of concept study
dc.creator.fl_str_mv Rubio Carrasquilla, Marcela
Santa Escobar, Cristian David
Rondón Marín, Juan Pablo
Botero Garcés, Jorge Humberto
Guimaraes, Allan J.
Moreno Frias, Ernesto
Cano Restrepo, Luz Elena
dc.contributor.author.none.fl_str_mv Rubio Carrasquilla, Marcela
Santa Escobar, Cristian David
Rondón Marín, Juan Pablo
Botero Garcés, Jorge Humberto
Guimaraes, Allan J.
Moreno Frias, Ernesto
Cano Restrepo, Luz Elena
dc.subject.decs.none.fl_str_mv Antígenos Fúngicos
Antigens, Fungal
Histoplasmosis
Histoplasma
Histoplasmosis
Ensayos de Liberación de Interferón gamma
Interferon-gamma Release Tests
Valor Predictivo de las Pruebas
Predictive Value of Tests
Prueba de Estudio Conceptual
Proof of Concept Study
topic Antígenos Fúngicos
Antigens, Fungal
Histoplasmosis
Histoplasma
Histoplasmosis
Ensayos de Liberación de Interferón gamma
Interferon-gamma Release Tests
Valor Predictivo de las Pruebas
Predictive Value of Tests
Prueba de Estudio Conceptual
Proof of Concept Study
description ABSTRACT : Histoplasmosis is the most common endemic mycosis in the Americas. Currently, there is no laboratory test capable to detect subclinical or latent infections by Histoplasma capsulatum (Hc), which might develop as severe infections in immunocompromised individuals. For the first time to our knowledge, we explore the suitability of an interferon gamma release assay (IGRA) to detect latent Hc infection in asymptomatic individuals. A cohort of 126 volunteers was enrolled in the study, 13 of which underwent a Hc infection in the past, and 93 of them showing risk factors for this infection. The remaining 20 participants did not refer any risk factors of Hc infection, but eight of them showed evidences of infection with Mycobacterium tuberculosis. All participants were recruited in Medellin, Colombia, between January 2014 and December 2017. Whole blood samples were cultured with four different Hc crude antigens and phytohemaglutinin as positive control. The interferon (IFN)-γ released by T lymphocytes upon antigen stimulation was quantified by ELISA. A defined cutoff value of 20 pg/ml for the IFN-γ concentration allowed us to distinguish between the group with documented past infections and the group of noninfected individuals with high sensitivity (70– 92%) and specificity (85–95%), for the four tested antigens. Positive 82–95% and negative 77–92% predictive values were also very high, comparable to those reported for commercially available IGRAs. The new test constitutes a promising screening method to detect individuals with latent Hc infection, even decades after the primary infection, as evidenced in this study.
publishDate 2018
dc.date.issued.none.fl_str_mv 2018
dc.date.accessioned.none.fl_str_mv 2022-03-14T16:58:20Z
dc.date.available.none.fl_str_mv 2022-03-14T16:58:20Z
dc.type.spa.fl_str_mv info:eu-repo/semantics/article
dc.type.hasversion.spa.fl_str_mv info:eu-repo/semantics/acceptedVersion
dc.type.coar.spa.fl_str_mv http://purl.org/coar/resource_type/c_2df8fbb1
dc.type.redcol.spa.fl_str_mv https://purl.org/redcol/resource_type/ART
dc.type.local.spa.fl_str_mv Artículo de investigación
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dc.identifier.citation.spa.fl_str_mv Rubio-Carrasquilla M, Santa CD, Rendón JP, Botero-Garcés J, Guimarães AJ, Moreno E, Cano LE. An interferon gamma release assay specific for Histoplasma capsulatum to detect asymptomatic infected individuals: A proof of concept study. Med Mycol. 2019 Aug 1;57(6):724-732. doi: 10.1093/mmy/myy131. Rubio-Carrasquilla M, Santa CD, Rendón JP, Botero-Garcés J, Guimarães AJ, Moreno E, Cano LE. An interferon gamma release assay specific for Histoplasma capsulatum to detect asymptomatic infected individuals: A proof of concept study. Med Mycol. 2019 Aug 1;57(6):724-732. doi: 10.1093/mmy/myy131.
dc.identifier.issn.none.fl_str_mv 1369-3786
dc.identifier.uri.none.fl_str_mv http://hdl.handle.net/10495/26552
dc.identifier.doi.none.fl_str_mv 10.1093/mmy/myy131
dc.identifier.eissn.none.fl_str_mv 1460-2709
identifier_str_mv Rubio-Carrasquilla M, Santa CD, Rendón JP, Botero-Garcés J, Guimarães AJ, Moreno E, Cano LE. An interferon gamma release assay specific for Histoplasma capsulatum to detect asymptomatic infected individuals: A proof of concept study. Med Mycol. 2019 Aug 1;57(6):724-732. doi: 10.1093/mmy/myy131. Rubio-Carrasquilla M, Santa CD, Rendón JP, Botero-Garcés J, Guimarães AJ, Moreno E, Cano LE. An interferon gamma release assay specific for Histoplasma capsulatum to detect asymptomatic infected individuals: A proof of concept study. Med Mycol. 2019 Aug 1;57(6):724-732. doi: 10.1093/mmy/myy131.
1369-3786
10.1093/mmy/myy131
1460-2709
url http://hdl.handle.net/10495/26552
dc.language.iso.spa.fl_str_mv eng
language eng
dc.relation.ispartofjournalabbrev.spa.fl_str_mv Med. Mycol.
dc.rights.spa.fl_str_mv info:eu-repo/semantics/openAccess
dc.rights.uri.*.fl_str_mv http://creativecommons.org/licenses/by-nc/2.5/co/
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dc.rights.creativecommons.spa.fl_str_mv https://creativecommons.org/licenses/by-nc/4.0/
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc/2.5/co/
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dc.format.extent.spa.fl_str_mv 9
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dc.publisher.spa.fl_str_mv Oxford University Press
dc.publisher.group.spa.fl_str_mv Grupo de Parasitología Universidad de Antioquia
Micología Médica y Experimental
dc.publisher.place.spa.fl_str_mv Oxford, Inglaterra
institution Universidad de Antioquia
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spelling Rubio Carrasquilla, MarcelaSanta Escobar, Cristian DavidRondón Marín, Juan PabloBotero Garcés, Jorge HumbertoGuimaraes, Allan J.Moreno Frias, ErnestoCano Restrepo, Luz Elena2022-03-14T16:58:20Z2022-03-14T16:58:20Z2018Rubio-Carrasquilla M, Santa CD, Rendón JP, Botero-Garcés J, Guimarães AJ, Moreno E, Cano LE. An interferon gamma release assay specific for Histoplasma capsulatum to detect asymptomatic infected individuals: A proof of concept study. Med Mycol. 2019 Aug 1;57(6):724-732. doi: 10.1093/mmy/myy131. Rubio-Carrasquilla M, Santa CD, Rendón JP, Botero-Garcés J, Guimarães AJ, Moreno E, Cano LE. An interferon gamma release assay specific for Histoplasma capsulatum to detect asymptomatic infected individuals: A proof of concept study. Med Mycol. 2019 Aug 1;57(6):724-732. doi: 10.1093/mmy/myy131.1369-3786http://hdl.handle.net/10495/2655210.1093/mmy/myy1311460-2709ABSTRACT : Histoplasmosis is the most common endemic mycosis in the Americas. Currently, there is no laboratory test capable to detect subclinical or latent infections by Histoplasma capsulatum (Hc), which might develop as severe infections in immunocompromised individuals. For the first time to our knowledge, we explore the suitability of an interferon gamma release assay (IGRA) to detect latent Hc infection in asymptomatic individuals. A cohort of 126 volunteers was enrolled in the study, 13 of which underwent a Hc infection in the past, and 93 of them showing risk factors for this infection. The remaining 20 participants did not refer any risk factors of Hc infection, but eight of them showed evidences of infection with Mycobacterium tuberculosis. All participants were recruited in Medellin, Colombia, between January 2014 and December 2017. Whole blood samples were cultured with four different Hc crude antigens and phytohemaglutinin as positive control. The interferon (IFN)-γ released by T lymphocytes upon antigen stimulation was quantified by ELISA. A defined cutoff value of 20 pg/ml for the IFN-γ concentration allowed us to distinguish between the group with documented past infections and the group of noninfected individuals with high sensitivity (70– 92%) and specificity (85–95%), for the four tested antigens. Positive 82–95% and negative 77–92% predictive values were also very high, comparable to those reported for commercially available IGRAs. The new test constitutes a promising screening method to detect individuals with latent Hc infection, even decades after the primary infection, as evidenced in this study.COL0013709COL00075069application/pdfengOxford University PressGrupo de Parasitología Universidad de AntioquiaMicología Médica y ExperimentalOxford, Inglaterrainfo:eu-repo/semantics/acceptedVersioninfo:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_2df8fbb1https://purl.org/redcol/resource_type/ARTArtículo de investigacióninfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc/2.5/co/http://purl.org/coar/access_right/c_abf2https://creativecommons.org/licenses/by-nc/4.0/An interferon gamma release assay specific for Histoplasma capsulatum to detect asymptomatic infected individuals: A proof of concept studyAntígenos FúngicosAntigens, FungalHistoplasmosisHistoplasmaHistoplasmosisEnsayos de Liberación de Interferón gammaInterferon-gamma Release TestsValor Predictivo de las PruebasPredictive Value of TestsPrueba de Estudio ConceptualProof of Concept StudyMed. Mycol.Medical Mycology724732576CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8933http://bibliotecadigital.udea.edu.co/bitstream/10495/26552/2/license_rdfc0c92b0ffc8b7d22d9cf56754a416a76MD52ORIGINALRubioMarcela_2018_HistoplasmaCapsulatum.pdfRubioMarcela_2018_HistoplasmaCapsulatum.pdfArtículo de investigaciónapplication/pdf1039021http://bibliotecadigital.udea.edu.co/bitstream/10495/26552/1/RubioMarcela_2018_HistoplasmaCapsulatum.pdfe01bbeb2a02658d20d30e95d0aba9fe4MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748http://bibliotecadigital.udea.edu.co/bitstream/10495/26552/3/license.txt8a4605be74aa9ea9d79846c1fba20a33MD5310495/26552oai:bibliotecadigital.udea.edu.co:10495/265522022-04-22 10:24:17.588Repositorio Institucional Universidad de Antioquiaandres.perez@udea.edu.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