Transcriptional reprogramming of gene expression in bovine somatic cell chromatin transfer embryos

ABSTRACT: Background: Successful reprogramming of a somatic genome to produce a healthy clone by somatic cells nuclear transfer (SCNT) is a rare event and the mechanisms involved in this process are poorly defined. When serial or successive rounds of cloning are performed, blastocyst and full term development rates decline even further with the increasing rounds of cloning. Identifying the "cumulative errors" could reveal the epigenetic reprogramming blocks in animal cloning.

• Autores: Rodríguez Osorio, Nélida; Wang, Zhongde; Kasinathan, Poothappillai; Page, Grier P.; Robl, James M.; Memili, Erdogan
• Tipo de recurso: Article of investigation
• Fecha de publicación: 2009
• Institución: Universidad de Antioquia
• Repositorio: Repositorio UdeA
• Idioma: eng
• OAI Identifier: oai:bibliotecadigital.udea.edu.co:10495/33391
• Acceso en línea: https://hdl.handle.net/10495/33391
• Palabra clave: Bovinos; Cattle; Blastocisto; Blastocyst; Línea Celular; Cell Line; Reprogramación Celular; Cellular Reprogramming; Clonación de Organismos; Cloning, Organism; Análisis por Conglomerados; Cluster Analysis; Embrión de Mamíferos; Embryo, Mammalian; Fertilización In Vitro; Fertilization in Vitro; Perfilación de la Expresión Génica; Gene Expression Profiling; Regulación del Desarrollo de la Expresión Génica; Gene Expression Regulation, Developmental; Reacción en Cadena de la Polimerasa de Transcriptasa Inversa; Reverse Transcriptase Polymerase Chain Reaction; Técnicas de Transferencia Nuclear; Nuclear Transfer Techniques; Análisis de Secuencia por Matrices de Oligonucleótidos; Oligonucleotide Array Sequence Analysis; Oocitos; Oocytes
• Rights: openAccess
• License: https://creativecommons.org/licenses/by/4.0/