Encuesta virológica del Herpesvirus bovino-1 (HVB-1) en la Feria de Ganados de Medellín

ABSTRACT: Virological survey of Bovine Herpesvirus-1 (BHV-1) at the cattle fair in Medellin-Antioquia, Colombia. In order to contribute to the characterization of infectious Bovine Rhinotracheitis virus in Colombia, and attempt was made to isolate BHV-1 from 716 nasal, vaginal or preputial swabs tak...

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Autores:
Restrepo Metaute, Martin Edgar
Rodas González, Juan David
Mogollón Torres, Martha
Zuluaga Tobón, Fabio Nelson
Ossa Londoño, Jorge Eliecer
Tipo de recurso:
Article of investigation
Fecha de publicación:
1996
Institución:
Universidad de Antioquia
Repositorio:
Repositorio UdeA
Idioma:
spa
OAI Identifier:
oai:bibliotecadigital.udea.edu.co:10495/6293
Acceso en línea:
http://hdl.handle.net/10495/6293
Palabra clave:
Bovinos
Herpesvirus
Ferias y exposiciones
Rights
openAccess
License
Atribución-NoComercial-CompartirIgual 2.5 Colombia (CC BY-NC-SA 2.5 CO)
id UDEA2_c3c803baaaf87c6e4bcf589fd00c92a4
oai_identifier_str oai:bibliotecadigital.udea.edu.co:10495/6293
network_acronym_str UDEA2
network_name_str Repositorio UdeA
repository_id_str
dc.title.spa.fl_str_mv Encuesta virológica del Herpesvirus bovino-1 (HVB-1) en la Feria de Ganados de Medellín
dc.title.alternative.spa.fl_str_mv Virological survey of Bovine Herpesvirus - 1 (BHV-1) at the cattle fair in medellin - Antioquia, Colombia
title Encuesta virológica del Herpesvirus bovino-1 (HVB-1) en la Feria de Ganados de Medellín
spellingShingle Encuesta virológica del Herpesvirus bovino-1 (HVB-1) en la Feria de Ganados de Medellín
Bovinos
Herpesvirus
Ferias y exposiciones
title_short Encuesta virológica del Herpesvirus bovino-1 (HVB-1) en la Feria de Ganados de Medellín
title_full Encuesta virológica del Herpesvirus bovino-1 (HVB-1) en la Feria de Ganados de Medellín
title_fullStr Encuesta virológica del Herpesvirus bovino-1 (HVB-1) en la Feria de Ganados de Medellín
title_full_unstemmed Encuesta virológica del Herpesvirus bovino-1 (HVB-1) en la Feria de Ganados de Medellín
title_sort Encuesta virológica del Herpesvirus bovino-1 (HVB-1) en la Feria de Ganados de Medellín
dc.creator.fl_str_mv Restrepo Metaute, Martin Edgar
Rodas González, Juan David
Mogollón Torres, Martha
Zuluaga Tobón, Fabio Nelson
Ossa Londoño, Jorge Eliecer
dc.contributor.author.none.fl_str_mv Restrepo Metaute, Martin Edgar
Rodas González, Juan David
Mogollón Torres, Martha
Zuluaga Tobón, Fabio Nelson
Ossa Londoño, Jorge Eliecer
dc.subject.none.fl_str_mv Bovinos
Herpesvirus
Ferias y exposiciones
topic Bovinos
Herpesvirus
Ferias y exposiciones
description ABSTRACT: Virological survey of Bovine Herpesvirus-1 (BHV-1) at the cattle fair in Medellin-Antioquia, Colombia. In order to contribute to the characterization of infectious Bovine Rhinotracheitis virus in Colombia, and attempt was made to isolate BHV-1 from 716 nasal, vaginal or preputial swabs taken during a period of six months, from 480 animals transported from long distance to the cattle fair in Medellin. Samples were inoculated on MDBK cell monolayer. It was supposed that long distance transportation would induce stress with the consequence of viral reactivation and shedding. A total of 1044 attempt were made to isolate the virus including 328 re-inoculations. No virus could be isolated from the inoculated samples. In addition nasal, vaginal or preputial samples were tested from 38 animals from a herds with a make of abortion located in Magangue (Bolivar), at the north cost of Colombia among the tested their were 25 seropositive, but again there were no isolations. However three semen samples out of 4 taken from bulls in this herd were positive by PCR. Further research is needed to define the real effect of BHV-1 infection in Colombia and particularly to the characterize biochemistry pattern of local strains and its pathogenic behavior.
publishDate 1996
dc.date.issued.none.fl_str_mv 1996
dc.date.accessioned.none.fl_str_mv 2017-02-19T18:36:43Z
dc.date.available.none.fl_str_mv 2017-02-19T18:36:43Z
dc.type.spa.fl_str_mv info:eu-repo/semantics/article
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dc.type.local.spa.fl_str_mv Artículo de investigación
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dc.identifier.citation.spa.fl_str_mv Restrepo M, Rodas JD, Mogollon M, Zuluaga FN, Ossa JE. Encuesta virológica del Herpesvirus bovino-1 (HVB-1) en la Feria de Ganados de Medellín. Rev. colomb. cienc. pecu. 1996;9(1):58–64
dc.identifier.issn.none.fl_str_mv 0120-0690
dc.identifier.uri.none.fl_str_mv http://hdl.handle.net/10495/6293
dc.identifier.eissn.none.fl_str_mv 2256-2958
identifier_str_mv Restrepo M, Rodas JD, Mogollon M, Zuluaga FN, Ossa JE. Encuesta virológica del Herpesvirus bovino-1 (HVB-1) en la Feria de Ganados de Medellín. Rev. colomb. cienc. pecu. 1996;9(1):58–64
0120-0690
2256-2958
url http://hdl.handle.net/10495/6293
dc.language.iso.spa.fl_str_mv spa
language spa
dc.relation.ispartofjournalabbrev.spa.fl_str_mv Rev. Colomb. Cienc. Pecu.
dc.rights.*.fl_str_mv Atribución-NoComercial-CompartirIgual 2.5 Colombia (CC BY-NC-SA 2.5 CO)
dc.rights.spa.fl_str_mv info:eu-repo/semantics/openAccess
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rights_invalid_str_mv Atribución-NoComercial-CompartirIgual 2.5 Colombia (CC BY-NC-SA 2.5 CO)
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eu_rights_str_mv openAccess
dc.format.mimetype.spa.fl_str_mv application/pdf
dc.publisher.spa.fl_str_mv Universidad de Antioquia, Facultad de Ciencias Agrarias
dc.publisher.place.spa.fl_str_mv Medellín, Colombia
institution Universidad de Antioquia
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spelling Restrepo Metaute, Martin EdgarRodas González, Juan DavidMogollón Torres, MarthaZuluaga Tobón, Fabio NelsonOssa Londoño, Jorge Eliecer2017-02-19T18:36:43Z2017-02-19T18:36:43Z1996Restrepo M, Rodas JD, Mogollon M, Zuluaga FN, Ossa JE. Encuesta virológica del Herpesvirus bovino-1 (HVB-1) en la Feria de Ganados de Medellín. Rev. colomb. cienc. pecu. 1996;9(1):58–640120-0690http://hdl.handle.net/10495/62932256-2958ABSTRACT: Virological survey of Bovine Herpesvirus-1 (BHV-1) at the cattle fair in Medellin-Antioquia, Colombia. In order to contribute to the characterization of infectious Bovine Rhinotracheitis virus in Colombia, and attempt was made to isolate BHV-1 from 716 nasal, vaginal or preputial swabs taken during a period of six months, from 480 animals transported from long distance to the cattle fair in Medellin. Samples were inoculated on MDBK cell monolayer. It was supposed that long distance transportation would induce stress with the consequence of viral reactivation and shedding. A total of 1044 attempt were made to isolate the virus including 328 re-inoculations. No virus could be isolated from the inoculated samples. In addition nasal, vaginal or preputial samples were tested from 38 animals from a herds with a make of abortion located in Magangue (Bolivar), at the north cost of Colombia among the tested their were 25 seropositive, but again there were no isolations. However three semen samples out of 4 taken from bulls in this herd were positive by PCR. Further research is needed to define the real effect of BHV-1 infection in Colombia and particularly to the characterize biochemistry pattern of local strains and its pathogenic behavior.RESUMEN: Con el objeto general de contribuir a la caracterización de la rinotraqueitis infecciosa bovina en Colombia, intentamos aislar el HVB-1 a partir de 716 hisopados nasales, vaginales o prepuciales tomados de 480 bovinos, en la feria de ganado de Medellín, bajo el supuesto de que el estrés del largo viaje desde diferentes sitios geográficos del país y el ayuno al que son sometidos estos animales favorecieran la excreción viral. Se hicieron 1044 intentos de aislamientos, 328 de ellos correspondieron a re inoculaciones o pases ciegos de las mismas muestras y en ningún caso se obtuvo evidencia de la presencia del virus. Adicionalmente se tomaron 38 hisopados nasales, vaginales o prepuciales a partir de bovinos de hatos seropositivos de la costa norte de Colombia (Municipio de Magangué-Departamento de Bolívar), de haciendas con alta incidencia de aborto. En estos animales el porcentaje de seropositividad por la técnica de ELLISA fue de 67%, sin embargo, tampoco en estas muestra fue posible aislar el virus; a pesar de que por técnicas de PCR, hubo evidencia de la presencia del virus en el semen de 3 toros seropositivos. Es necesario continuar esta línea de trabajo para definir el verdadero impacto de esta infección en el país y muy particularmente para hacer la caracterización bioquímica de las cepas virales autóctonas y su comportamiento desde el punto de vista patológico.application/pdfspaUniversidad de Antioquia, Facultad de Ciencias AgrariasMedellín, Colombiainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_2df8fbb1https://purl.org/redcol/resource_type/ARTArtículo de investigaciónhttp://purl.org/coar/version/c_970fb48d4fbd8a85Atribución-NoComercial-CompartirIgual 2.5 Colombia (CC BY-NC-SA 2.5 CO)info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/co/http://purl.org/coar/access_right/c_abf2https://creativecommons.org/licenses/by-nc-sa/4.0/BovinosHerpesvirusFerias y exposicionesEncuesta virológica del Herpesvirus bovino-1 (HVB-1) en la Feria de Ganados de MedellínVirological survey of Bovine Herpesvirus - 1 (BHV-1) at the cattle fair in medellin - Antioquia, ColombiaRev. Colomb. Cienc. Pecu.Revista Colombiana de Ciencias Pecuarias586491ORIGINALRodas_D_juan_1996_Encuesta_Herpesvirus_bovino-1.pdfRodas_D_juan_1996_Encuesta_Herpesvirus_bovino-1.pdfArtículo de investigaciónapplication/pdf1593633http://bibliotecadigital.udea.edu.co/bitstream/10495/6293/6/Rodas_D_juan_1996_Encuesta_Herpesvirus_bovino-1.pdfd6aff258a44a86a34f8414d3b92ef066MD56CC-LICENSElicense_urllicense_urltext/plain; charset=utf-849http://bibliotecadigital.udea.edu.co/bitstream/10495/6293/7/license_url4afdbb8c545fd630ea7db775da747b2fMD57license_textlicense_texttext/html; charset=utf-80http://bibliotecadigital.udea.edu.co/bitstream/10495/6293/8/license_textd41d8cd98f00b204e9800998ecf8427eMD58license_rdflicense_rdfapplication/rdf+xml; charset=utf-80http://bibliotecadigital.udea.edu.co/bitstream/10495/6293/9/license_rdfd41d8cd98f00b204e9800998ecf8427eMD59LICENSElicense.txtlicense.txttext/plain; charset=utf-81748http://bibliotecadigital.udea.edu.co/bitstream/10495/6293/10/license.txt8a4605be74aa9ea9d79846c1fba20a33MD51010495/6293oai:bibliotecadigital.udea.edu.co:10495/62932021-05-21 20:25:51.471Repositorio Institucional Universidad de Antioquiaandres.perez@udea.edu.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