Maintenance of red-tail coral snake (Micrurus mipartitus) in captivity and evaluation of individual venom variability

ABSTRACT: Red-tail coral snake (Micrurus mipartitus) is a long and thin bicolor coral snake widely distributed in Colombia and is the coral that causes the majority of accidents in the Andean region, so it is important to keep this species in captivity for anti-venom production and research. However...

Full description

Autores:
Henao Duque, Ana María
Núñez Rangel, Vitelbina
Tipo de recurso:
Article of investigation
Fecha de publicación:
2016
Institución:
Universidad de Antioquia
Repositorio:
Repositorio UdeA
Idioma:
eng
OAI Identifier:
oai:bibliotecadigital.udea.edu.co:10495/20258
Acceso en línea:
http://hdl.handle.net/10495/20258
Palabra clave:
Serpientes
Serpentes
Víbora
Vipers
Alimentación forzada
Forced feeding
Mortalidad
Mortality
Antivenom
Coral rabo de ají : Micrurus mipartitus
Antiveneno
http://aims.fao.org/aos/agrovoc/c_14209
http://aims.fao.org/aos/agrovoc/c_15208
http://aims.fao.org/aos/agrovoc/c_3038
http://aims.fao.org/aos/agrovoc/c_4945
Rights
openAccess
License
http://creativecommons.org/licenses/by-nc-sa/2.5/co/
id UDEA2_c36027f8d74c539e2a00786f343b341c
oai_identifier_str oai:bibliotecadigital.udea.edu.co:10495/20258
network_acronym_str UDEA2
network_name_str Repositorio UdeA
repository_id_str
dc.title.spa.fl_str_mv Maintenance of red-tail coral snake (Micrurus mipartitus) in captivity and evaluation of individual venom variability
dc.title.alternative.spa.fl_str_mv Mantenimiento en cautiverio de la coral rabo de ají (Micrurus mipartitus) y evaluación en la variabilidad individual de su veneno
title Maintenance of red-tail coral snake (Micrurus mipartitus) in captivity and evaluation of individual venom variability
spellingShingle Maintenance of red-tail coral snake (Micrurus mipartitus) in captivity and evaluation of individual venom variability
Serpientes
Serpentes
Víbora
Vipers
Alimentación forzada
Forced feeding
Mortalidad
Mortality
Antivenom
Coral rabo de ají : Micrurus mipartitus
Antiveneno
http://aims.fao.org/aos/agrovoc/c_14209
http://aims.fao.org/aos/agrovoc/c_15208
http://aims.fao.org/aos/agrovoc/c_3038
http://aims.fao.org/aos/agrovoc/c_4945
title_short Maintenance of red-tail coral snake (Micrurus mipartitus) in captivity and evaluation of individual venom variability
title_full Maintenance of red-tail coral snake (Micrurus mipartitus) in captivity and evaluation of individual venom variability
title_fullStr Maintenance of red-tail coral snake (Micrurus mipartitus) in captivity and evaluation of individual venom variability
title_full_unstemmed Maintenance of red-tail coral snake (Micrurus mipartitus) in captivity and evaluation of individual venom variability
title_sort Maintenance of red-tail coral snake (Micrurus mipartitus) in captivity and evaluation of individual venom variability
dc.creator.fl_str_mv Henao Duque, Ana María
Núñez Rangel, Vitelbina
dc.contributor.author.none.fl_str_mv Henao Duque, Ana María
Núñez Rangel, Vitelbina
dc.subject.agrovoc.none.fl_str_mv Serpientes
Serpentes
Víbora
Vipers
Alimentación forzada
Forced feeding
Mortalidad
Mortality
topic Serpientes
Serpentes
Víbora
Vipers
Alimentación forzada
Forced feeding
Mortalidad
Mortality
Antivenom
Coral rabo de ají : Micrurus mipartitus
Antiveneno
http://aims.fao.org/aos/agrovoc/c_14209
http://aims.fao.org/aos/agrovoc/c_15208
http://aims.fao.org/aos/agrovoc/c_3038
http://aims.fao.org/aos/agrovoc/c_4945
dc.subject.proposal.spa.fl_str_mv Antivenom
Coral rabo de ají : Micrurus mipartitus
Antiveneno
dc.subject.agrovocuri.none.fl_str_mv http://aims.fao.org/aos/agrovoc/c_14209
http://aims.fao.org/aos/agrovoc/c_15208
http://aims.fao.org/aos/agrovoc/c_3038
http://aims.fao.org/aos/agrovoc/c_4945
description ABSTRACT: Red-tail coral snake (Micrurus mipartitus) is a long and thin bicolor coral snake widely distributed in Colombia and is the coral that causes the majority of accidents in the Andean region, so it is important to keep this species in captivity for anti-venom production and research. However, maintaining this species in captivity is very difficult because it refuses to feed, in addition to the high mortality rate due to maladaptation syndrome. In this study a force feeding diet, diverse substrates for maintenance and a milking technique were evaluated. Additionally, individual variability of the venom was determined by High Performance Liquid Chromatography (HPLC), Sodium Dodecyl Sulfate- Polyacrylamide Gel Electrophoresis (SDS-PAGE) and Coagulant, Anticoagulant and Hemolytic activities. The results of this study demonstrate that it was possible to increase the survival rate of this species in captivity and to determine some of the important factors in the maintenance. As to the individual variability of the venom, we found differences in number and intensity of peaks recovered by chromatography and also displayed variations in some of its biological activities.
publishDate 2016
dc.date.issued.none.fl_str_mv 2016
dc.date.accessioned.none.fl_str_mv 2021-06-23T01:24:22Z
dc.date.available.none.fl_str_mv 2021-06-23T01:24:22Z
dc.type.spa.fl_str_mv info:eu-repo/semantics/article
dc.type.coarversion.fl_str_mv http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.hasversion.spa.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.coar.spa.fl_str_mv http://purl.org/coar/resource_type/c_2df8fbb1
dc.type.redcol.spa.fl_str_mv https://purl.org/redcol/resource_type/ART
dc.type.local.spa.fl_str_mv Artículo de investigación
format http://purl.org/coar/resource_type/c_2df8fbb1
status_str publishedVersion
dc.identifier.issn.none.fl_str_mv 0120-548X
dc.identifier.uri.none.fl_str_mv http://hdl.handle.net/10495/20258
dc.identifier.doi.none.fl_str_mv 10.15446/abc.v21n3.51651
dc.identifier.eissn.none.fl_str_mv 1900-1649
identifier_str_mv 0120-548X
10.15446/abc.v21n3.51651
1900-1649
url http://hdl.handle.net/10495/20258
dc.language.iso.spa.fl_str_mv eng
language eng
dc.relation.ispartofjournalabbrev.spa.fl_str_mv Acta Biolo Colomb
dc.rights.spa.fl_str_mv info:eu-repo/semantics/openAccess
dc.rights.uri.*.fl_str_mv http://creativecommons.org/licenses/by-nc-sa/2.5/co/
dc.rights.accessrights.spa.fl_str_mv http://purl.org/coar/access_right/c_abf2
dc.rights.creativecommons.spa.fl_str_mv https://creativecommons.org/licenses/by-nc-sa/4.0/
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/2.5/co/
http://purl.org/coar/access_right/c_abf2
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dc.format.extent.spa.fl_str_mv 8
dc.format.mimetype.spa.fl_str_mv application/pdf
dc.publisher.spa.fl_str_mv Universidad Nacional de Colombia, Facultad de Ciencias, Departamento de Biología
dc.publisher.group.spa.fl_str_mv Toxinología Alternativas Terapéuticas y Alimentarias
dc.publisher.place.spa.fl_str_mv Bogotá, Colombia
institution Universidad de Antioquia
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http://bibliotecadigital.udea.edu.co/bitstream/10495/20258/3/license.txt
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bitstream.checksumAlgorithm.fl_str_mv MD5
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repository.name.fl_str_mv Repositorio Institucional Universidad de Antioquia
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spelling Henao Duque, Ana MaríaNúñez Rangel, Vitelbina2021-06-23T01:24:22Z2021-06-23T01:24:22Z20160120-548Xhttp://hdl.handle.net/10495/2025810.15446/abc.v21n3.516511900-1649ABSTRACT: Red-tail coral snake (Micrurus mipartitus) is a long and thin bicolor coral snake widely distributed in Colombia and is the coral that causes the majority of accidents in the Andean region, so it is important to keep this species in captivity for anti-venom production and research. However, maintaining this species in captivity is very difficult because it refuses to feed, in addition to the high mortality rate due to maladaptation syndrome. In this study a force feeding diet, diverse substrates for maintenance and a milking technique were evaluated. Additionally, individual variability of the venom was determined by High Performance Liquid Chromatography (HPLC), Sodium Dodecyl Sulfate- Polyacrylamide Gel Electrophoresis (SDS-PAGE) and Coagulant, Anticoagulant and Hemolytic activities. The results of this study demonstrate that it was possible to increase the survival rate of this species in captivity and to determine some of the important factors in the maintenance. As to the individual variability of the venom, we found differences in number and intensity of peaks recovered by chromatography and also displayed variations in some of its biological activities.RESUMEN: La coral “rabo de ají” es una coral bicolor larga y delgada. Esta especie está ampliamente distribuida en Colombia y es la coral que causa el mayor número de accidentes en la región Andina, por esto es importante mantener esta especie en cautiverio con fines de producción de antivenenos e investigación. No obstante, el mantenimiento de esta especie en cautiverio es difícil, debido a que se rehúsan a alimentarse voluntariamente y a que presentan alta mortalidad por el denominado síndrome de mal adaptación. En este estudio se evaluaron varios sustratos para el mantenimiento, además de una dieta forzada y una técnica de ordeño. Adicionalmente, se evaluó la variabilidad individual del veneno a través de cromatografía liquida de alta eficiencia (HPLC), electroforesis (SDS-PAGE) y las actividades coagulante, anticoagulante y hemolítica indirecta. Los resultados de este estudio demostraron que fue posible incrementar la sobrevivencia de esta especie en cautiverio, así como determinar algunos factores importantes en su mantenimiento. A partir de la evaluación del veneno se encontraron diferencias en el número y en la intensidad de picos en la cromatografía, así como en algunas de sus actividades biológicas.COL00144768application/pdfengUniversidad Nacional de Colombia, Facultad de Ciencias, Departamento de BiologíaToxinología Alternativas Terapéuticas y AlimentariasBogotá, Colombiainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_2df8fbb1https://purl.org/redcol/resource_type/ARTArtículo de investigaciónhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/2.5/co/http://purl.org/coar/access_right/c_abf2https://creativecommons.org/licenses/by-nc-sa/4.0/Maintenance of red-tail coral snake (Micrurus mipartitus) in captivity and evaluation of individual venom variabilityMantenimiento en cautiverio de la coral rabo de ají (Micrurus mipartitus) y evaluación en la variabilidad individual de su venenoSerpientesSerpentesVíboraVipersAlimentación forzadaForced feedingMortalidadMortalityAntivenomCoral rabo de ají : Micrurus mipartitusAntivenenohttp://aims.fao.org/aos/agrovoc/c_14209http://aims.fao.org/aos/agrovoc/c_15208http://aims.fao.org/aos/agrovoc/c_3038http://aims.fao.org/aos/agrovoc/c_4945Acta Biolo ColombActa Biológica Colombiana593600213CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-81051http://bibliotecadigital.udea.edu.co/bitstream/10495/20258/2/license_rdfe2060682c9c70d4d30c83c51448f4eedMD52ORIGINALHenaoAna_2016_MaintenanceCoralSnake.pdfHenaoAna_2016_MaintenanceCoralSnake.pdfArtículo de investigaciónapplication/pdf318533http://bibliotecadigital.udea.edu.co/bitstream/10495/20258/1/HenaoAna_2016_MaintenanceCoralSnake.pdfb79a9d9fb71693890c5020805391a1adMD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748http://bibliotecadigital.udea.edu.co/bitstream/10495/20258/3/license.txt8a4605be74aa9ea9d79846c1fba20a33MD5310495/20258oai:bibliotecadigital.udea.edu.co:10495/202582021-06-22 20:24:22.62Repositorio Institucional Universidad de Antioquiaandres.perez@udea.edu.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