Cardiac glycosides, phenolic compounds and antioxidant activity from plant cell suspension cultures of Thevetia peruviana

ABSTRACT: Cell suspension cultures of Thevetia peruviana were established under dark for 19 days to investigate kinetic behavior related to biomass, substrate, cardiac glycoside, polyphenols, reactive oxygen species and anti-oxidant activity. The results showed high biomass production (18.80gDW/L) a...

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Autores:
Arias Echeverri, Juan Pablo
Zapata Acosta, Karol
Rojano, Benjamín
Peñuela Vásquez, Mariana
Arias Zabala, Mario
Tipo de recurso:
Article of investigation
Fecha de publicación:
2017
Institución:
Universidad de Antioquia
Repositorio:
Repositorio UdeA
Idioma:
eng
OAI Identifier:
oai:bibliotecadigital.udea.edu.co:10495/28035
Acceso en línea:
http://hdl.handle.net/10495/28035
Palabra clave:
Especies Reactivas de Oxígeno
Metabolitos vegetales
Plant metabolites
Antioxidantes
Antioxidants
Fenoles
Phenols
Cultivo de células vegetales
plant cell culture
Reactive Oxygen Species
Thevetia peruviana
Rights
openAccess
License
http://creativecommons.org/licenses/by-nc/2.5/co/
id UDEA2_bd6f55f36a513198cd6924a26a731b16
oai_identifier_str oai:bibliotecadigital.udea.edu.co:10495/28035
network_acronym_str UDEA2
network_name_str Repositorio UdeA
repository_id_str
dc.title.spa.fl_str_mv Cardiac glycosides, phenolic compounds and antioxidant activity from plant cell suspension cultures of Thevetia peruviana
dc.title.alternative.spa.fl_str_mv Producción de glícosidos cardiotónicos, compuestos fenólicos y actividad antioxidante en cultivos de células vegetales en suspensión de Thevetia peruviana
title Cardiac glycosides, phenolic compounds and antioxidant activity from plant cell suspension cultures of Thevetia peruviana
spellingShingle Cardiac glycosides, phenolic compounds and antioxidant activity from plant cell suspension cultures of Thevetia peruviana
Especies Reactivas de Oxígeno
Metabolitos vegetales
Plant metabolites
Antioxidantes
Antioxidants
Fenoles
Phenols
Cultivo de células vegetales
plant cell culture
Reactive Oxygen Species
Thevetia peruviana
title_short Cardiac glycosides, phenolic compounds and antioxidant activity from plant cell suspension cultures of Thevetia peruviana
title_full Cardiac glycosides, phenolic compounds and antioxidant activity from plant cell suspension cultures of Thevetia peruviana
title_fullStr Cardiac glycosides, phenolic compounds and antioxidant activity from plant cell suspension cultures of Thevetia peruviana
title_full_unstemmed Cardiac glycosides, phenolic compounds and antioxidant activity from plant cell suspension cultures of Thevetia peruviana
title_sort Cardiac glycosides, phenolic compounds and antioxidant activity from plant cell suspension cultures of Thevetia peruviana
dc.creator.fl_str_mv Arias Echeverri, Juan Pablo
Zapata Acosta, Karol
Rojano, Benjamín
Peñuela Vásquez, Mariana
Arias Zabala, Mario
dc.contributor.author.none.fl_str_mv Arias Echeverri, Juan Pablo
Zapata Acosta, Karol
Rojano, Benjamín
Peñuela Vásquez, Mariana
Arias Zabala, Mario
dc.subject.decs.none.fl_str_mv Especies Reactivas de Oxígeno
topic Especies Reactivas de Oxígeno
Metabolitos vegetales
Plant metabolites
Antioxidantes
Antioxidants
Fenoles
Phenols
Cultivo de células vegetales
plant cell culture
Reactive Oxygen Species
Thevetia peruviana
dc.subject.lemb.none.fl_str_mv Metabolitos vegetales
Plant metabolites
Antioxidantes
Antioxidants
Fenoles
Phenols
Cultivo de células vegetales
plant cell culture
dc.subject.agrovoc.none.fl_str_mv Reactive Oxygen Species
dc.subject.proposal.spa.fl_str_mv Thevetia peruviana
description ABSTRACT: Cell suspension cultures of Thevetia peruviana were established under dark for 19 days to investigate kinetic behavior related to biomass, substrate, cardiac glycoside, polyphenols, reactive oxygen species and anti-oxidant activity. The results showed high biomass production (18.80gDW/L) as well as sucrose consumption in 7 days. Preferential glucose over fructose consumption was observed. Intracellular production of cardiac glycosides reached 2.58mg DE/gDW at day 19. Highest extracellular production was reached between day 2 and 7 (6.19mg DE/L). Highest extracellular phenolic content was 80.61 ± 5.16mg GAE/L at day 7. Intracellular phenolic content increased to 2.76 ± 0.14mg GAE/gFW at day 7 and remained constant until day 19. ROS production at day 7 could be related to sucrose and glucose total consumption. Pearson Product-Moment Correlation Coefficient (ρ) showed that the phenolic compounds in cell suspension cultures of T. peruviana were responsible for the observed anti-oxidant activity. All together, these results give the first steps in metabolic behavior in cell suspension cultures of T. peruviana.
publishDate 2017
dc.date.issued.none.fl_str_mv 2017
dc.date.accessioned.none.fl_str_mv 2022-04-29T23:23:20Z
dc.date.available.none.fl_str_mv 2022-04-29T23:23:20Z
dc.type.spa.fl_str_mv info:eu-repo/semantics/article
dc.type.coarversion.fl_str_mv http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.hasversion.spa.fl_str_mv info:eu-repo/semantics/publishedVersion
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dc.type.local.spa.fl_str_mv Artículo de investigación
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dc.identifier.citation.spa.fl_str_mv Arias, J. P., Zapata, K., Rojano, B., Peñuela, M., & Arias, M. (2017). Producción de glícosidos cardiotónicos, compuestos fenólicos y actividad antioxidante en cultivos de células vegetales en suspensión de Thevetia peruviana. Revista U.D.C.A Actualidad & Divulgación Científica, 20(2), 353–362. https://doi.org/10.31910/rudca.v20.n2.2017.393
dc.identifier.issn.none.fl_str_mv 0123-4226
dc.identifier.uri.none.fl_str_mv http://hdl.handle.net/10495/28035
dc.identifier.doi.none.fl_str_mv 10.31910/rudca.v20.n2.2017.393
dc.identifier.eissn.none.fl_str_mv 2619-2551
identifier_str_mv Arias, J. P., Zapata, K., Rojano, B., Peñuela, M., & Arias, M. (2017). Producción de glícosidos cardiotónicos, compuestos fenólicos y actividad antioxidante en cultivos de células vegetales en suspensión de Thevetia peruviana. Revista U.D.C.A Actualidad & Divulgación Científica, 20(2), 353–362. https://doi.org/10.31910/rudca.v20.n2.2017.393
0123-4226
10.31910/rudca.v20.n2.2017.393
2619-2551
url http://hdl.handle.net/10495/28035
dc.language.iso.spa.fl_str_mv eng
language eng
dc.relation.ispartofjournalabbrev.spa.fl_str_mv Rev. UDCA Actual. Divulg. Cient.
dc.rights.spa.fl_str_mv info:eu-repo/semantics/openAccess
dc.rights.uri.*.fl_str_mv http://creativecommons.org/licenses/by-nc/2.5/co/
dc.rights.accessrights.spa.fl_str_mv http://purl.org/coar/access_right/c_abf2
dc.rights.creativecommons.spa.fl_str_mv https://creativecommons.org/licenses/by-nc/4.0/
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc/2.5/co/
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https://creativecommons.org/licenses/by-nc/4.0/
dc.format.extent.spa.fl_str_mv 10
dc.format.mimetype.spa.fl_str_mv application/pdf
dc.publisher.spa.fl_str_mv Universidad de Ciencias Aplicadas y Ambientales U.D.C.A
dc.publisher.group.spa.fl_str_mv Bioprocesos
dc.publisher.place.spa.fl_str_mv Bogotá, Colombia
institution Universidad de Antioquia
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repository.name.fl_str_mv Repositorio Institucional Universidad de Antioquia
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spelling Arias Echeverri, Juan PabloZapata Acosta, KarolRojano, BenjamínPeñuela Vásquez, MarianaArias Zabala, Mario2022-04-29T23:23:20Z2022-04-29T23:23:20Z2017Arias, J. P., Zapata, K., Rojano, B., Peñuela, M., & Arias, M. (2017). Producción de glícosidos cardiotónicos, compuestos fenólicos y actividad antioxidante en cultivos de células vegetales en suspensión de Thevetia peruviana. Revista U.D.C.A Actualidad & Divulgación Científica, 20(2), 353–362. https://doi.org/10.31910/rudca.v20.n2.2017.3930123-4226http://hdl.handle.net/10495/2803510.31910/rudca.v20.n2.2017.3932619-2551ABSTRACT: Cell suspension cultures of Thevetia peruviana were established under dark for 19 days to investigate kinetic behavior related to biomass, substrate, cardiac glycoside, polyphenols, reactive oxygen species and anti-oxidant activity. The results showed high biomass production (18.80gDW/L) as well as sucrose consumption in 7 days. Preferential glucose over fructose consumption was observed. Intracellular production of cardiac glycosides reached 2.58mg DE/gDW at day 19. Highest extracellular production was reached between day 2 and 7 (6.19mg DE/L). Highest extracellular phenolic content was 80.61 ± 5.16mg GAE/L at day 7. Intracellular phenolic content increased to 2.76 ± 0.14mg GAE/gFW at day 7 and remained constant until day 19. ROS production at day 7 could be related to sucrose and glucose total consumption. Pearson Product-Moment Correlation Coefficient (ρ) showed that the phenolic compounds in cell suspension cultures of T. peruviana were responsible for the observed anti-oxidant activity. All together, these results give the first steps in metabolic behavior in cell suspension cultures of T. peruviana.RESUMEN: Se establecieron cultivos en suspensión de la especie vegetal Thevetia peruviana en oscuridad, durante 19 días, para estudiar el comportamiento cinético de producción de biomasa, consumo de sustrato, producción de glicósidos cardiotónicos, polifenoles, especies reactivas de oxígeno y actividad antioxidante. Los resultados mostraron una alta producción de biomasa (18,80g PS/L), al igual que consumo total de sacarosa, a los 7 días de cultivo. Se observó un consumo preferencial de glucosa sobre fructosa durante todo el cultivo. La producción de glicósidos cardiotónicos intracelulares alcanzó valores de 2,58mg ED/g PS, al día 19. La mayor producción extracelular (6,19mg ED/L), se alcanzó entre los días 2 y 7. El mayor contenido de compuestos fenólicos extracelular fue de 80,61 ± 5,16mg GAE/L, en el día 7. El contenido de compuestos fenólicos intracelulares incrementó a 2,76 ± 0,14mg AGE/gPF, al día 7 y se mantuvo constante, hasta el día 19. La producción de EROs, al día 7, puede estar relacionada con el consumo total de sacarosa y glucosa. El coeficiente de correlación producto-momento de Pearson (ρ) indicó que los compuestos fenólicos en cultivos celulares en suspensión de T. peruviana eran los responsables de la actividad antioxidante observada. En conjunto, estos resultados brindan las primeras bases relacionadas al comportamiento metabólico de cultivos celulares en suspensión, de T. peruviana.COL002371510application/pdfengUniversidad de Ciencias Aplicadas y Ambientales U.D.C.ABioprocesosBogotá, Colombiainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_2df8fbb1https://purl.org/redcol/resource_type/ARTArtículo de investigaciónhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc/2.5/co/http://purl.org/coar/access_right/c_abf2https://creativecommons.org/licenses/by-nc/4.0/Cardiac glycosides, phenolic compounds and antioxidant activity from plant cell suspension cultures of Thevetia peruvianaProducción de glícosidos cardiotónicos, compuestos fenólicos y actividad antioxidante en cultivos de células vegetales en suspensión de Thevetia peruvianaEspecies Reactivas de OxígenoMetabolitos vegetalesPlant metabolitesAntioxidantesAntioxidantsFenolesPhenolsCultivo de células vegetalesplant cell cultureReactive Oxygen SpeciesThevetia peruvianaRev. UDCA Actual. Divulg. Cient.Revista U.D.C.A. Actualidad & Divulgación Científica353362202ORIGINALPeñuelaMariana_2017_CardiacGlycosidesPhenolic.pdfPeñuelaMariana_2017_CardiacGlycosidesPhenolic.pdfArtículo de investigaciónapplication/pdf354417http://bibliotecadigital.udea.edu.co/bitstream/10495/28035/1/Pe%c3%b1uelaMariana_2017_CardiacGlycosidesPhenolic.pdff9bb7d47d868829bf70dd0af1c53f092MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748http://bibliotecadigital.udea.edu.co/bitstream/10495/28035/3/license.txt8a4605be74aa9ea9d79846c1fba20a33MD53CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8933http://bibliotecadigital.udea.edu.co/bitstream/10495/28035/2/license_rdfc0c92b0ffc8b7d22d9cf56754a416a76MD5210495/28035oai:bibliotecadigital.udea.edu.co:10495/280352022-04-29 18:23:21.358Repositorio Institucional Universidad de Antioquiaandres.perez@udea.edu.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