Detección de enzimas extracelulares en cepas cubanas del complejo Metarhizium anisopliae con acción entomopatogénica contra Cylas formicarius Fabricius (Coleoptera: Brentidae)

ABSTRACT: This study reports the detection, by biochemical methods, of extracellular enzymes related to the biological activity of nine strains of the complex Metarhizium anisopliae (Metsch.) Sorokin. We determined the pathogenic capacity against the insect Cylas formicarius Fabricius at different c...

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Autores:: Gato Cárdenas, Yohana
Márquez Gutiérrez, María Elena
Baró Robaina, Yamilé
Calle Osorno, Jaime de Jesús

Tipo de recurso:: Article of investigation

Fecha de publicación:: 2017

Institución:: Universidad de Antioquia

Repositorio:: Repositorio UdeA

Idioma:: spa

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dc.title.spa.fl_str_mv	Detección de enzimas extracelulares en cepas cubanas del complejo Metarhizium anisopliae con acción entomopatogénica contra Cylas formicarius Fabricius (Coleoptera: Brentidae)
dc.title.alternative.spa.fl_str_mv	Detection of extracellular enzymes in cuban strains of Metarhizium anisopliae complex with entomopathogenic action against Cylas formicarius Fabricius (Coleoptera: Brentidae)
title	Detección de enzimas extracelulares en cepas cubanas del complejo Metarhizium anisopliae con acción entomopatogénica contra Cylas formicarius Fabricius (Coleoptera: Brentidae)
spellingShingle	Detección de enzimas extracelulares en cepas cubanas del complejo Metarhizium anisopliae con acción entomopatogénica contra Cylas formicarius Fabricius (Coleoptera: Brentidae) Control biológico Biocontrol Hongos Hongos de suelos Hongos entomopatógenos Entomopathogenic fungi Fungi Pathogenic activity Soil fungi
title_short	Detección de enzimas extracelulares en cepas cubanas del complejo Metarhizium anisopliae con acción entomopatogénica contra Cylas formicarius Fabricius (Coleoptera: Brentidae)
title_full	Detección de enzimas extracelulares en cepas cubanas del complejo Metarhizium anisopliae con acción entomopatogénica contra Cylas formicarius Fabricius (Coleoptera: Brentidae)
title_fullStr	Detección de enzimas extracelulares en cepas cubanas del complejo Metarhizium anisopliae con acción entomopatogénica contra Cylas formicarius Fabricius (Coleoptera: Brentidae)
title_full_unstemmed	Detección de enzimas extracelulares en cepas cubanas del complejo Metarhizium anisopliae con acción entomopatogénica contra Cylas formicarius Fabricius (Coleoptera: Brentidae)
title_sort	Detección de enzimas extracelulares en cepas cubanas del complejo Metarhizium anisopliae con acción entomopatogénica contra Cylas formicarius Fabricius (Coleoptera: Brentidae)
dc.creator.fl_str_mv	Gato Cárdenas, Yohana Márquez Gutiérrez, María Elena Baró Robaina, Yamilé Calle Osorno, Jaime de Jesús
dc.contributor.author.none.fl_str_mv	Gato Cárdenas, Yohana Márquez Gutiérrez, María Elena Baró Robaina, Yamilé Calle Osorno, Jaime de Jesús
dc.subject.none.fl_str_mv	Control biológico Biocontrol Hongos Hongos de suelos Hongos entomopatógenos Entomopathogenic fungi Fungi Pathogenic activity Soil fungi
topic	Control biológico Biocontrol Hongos Hongos de suelos Hongos entomopatógenos Entomopathogenic fungi Fungi Pathogenic activity Soil fungi
description	ABSTRACT: This study reports the detection, by biochemical methods, of extracellular enzymes related to the biological activity of nine strains of the complex Metarhizium anisopliae (Metsch.) Sorokin. We determined the pathogenic capacity against the insect Cylas formicarius Fabricius at different concentrations, calculated the lethal time and concentration, and analyzed both the presence of extracellular enzymes in the fungal strains as well as their relation to the entomopathogenic effect and virulence against C. formicarius. All strains of the M. anisopliae complex studied were pathogenic with an enzymatic activity index greater than 1. The LBM-30 strain was the most effective with presence of protease, chitinase, caseinase, amylase and lipase enzymes. This result corroborates the relationship between of the enzymatic activity with the observed virulence.
publishDate	2017
dc.date.issued.none.fl_str_mv	2017
dc.date.accessioned.none.fl_str_mv	2018-10-02T22:01:39Z
dc.date.available.none.fl_str_mv	2018-10-02T22:01:39Z
dc.type.spa.fl_str_mv	info:eu-repo/semantics/article
dc.type.coarversion.fl_str_mv	http://purl.org/coar/version/c_970fb48d4fbd8a85
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dc.type.local.spa.fl_str_mv	Artículo de investigación
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dc.identifier.citation.spa.fl_str_mv	Gato Y, Márquez M, Baró Y, Calle J. Detección de enzimas extracelulares en cepas cubanas del complejo Metarhizium anisopliae con acción entomopatogénica contra Cylas formicarius Fabricius (Coleoptera: Brentidae). Actual Bio. 2017; 39(106):71-78. DOI: 10.17533/udea.acbi.v39n106a07
dc.identifier.issn.none.fl_str_mv	0304-3584
dc.identifier.uri.none.fl_str_mv	http://hdl.handle.net/10495/10170
dc.identifier.doi.none.fl_str_mv	10.17533/udea.acbi.v39n106a07
dc.identifier.eissn.none.fl_str_mv	2145-7166
identifier_str_mv	Gato Y, Márquez M, Baró Y, Calle J. Detección de enzimas extracelulares en cepas cubanas del complejo Metarhizium anisopliae con acción entomopatogénica contra Cylas formicarius Fabricius (Coleoptera: Brentidae). Actual Bio. 2017; 39(106):71-78. DOI: 10.17533/udea.acbi.v39n106a07 0304-3584 10.17533/udea.acbi.v39n106a07 2145-7166
url	http://hdl.handle.net/10495/10170
dc.language.iso.spa.fl_str_mv	spa
language	spa
dc.relation.ispartofjournalabbrev.spa.fl_str_mv	Actual. Biol.
dc.rights.*.fl_str_mv	Atribución-NoComercial-CompartirIgual 2.5 Colombia (CC BY-NC-SA 2.5 CO)
dc.rights.spa.fl_str_mv	info:eu-repo/semantics/openAccess
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eu_rights_str_mv	openAccess
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dc.publisher.spa.fl_str_mv	Universidad de Antioquia, Facultad de Ciencias Exactas y Naturales, Instituto de Biología
dc.publisher.group.spa.fl_str_mv	Biocontrol y Microbiología Ambiental -BIOMA-
dc.publisher.place.spa.fl_str_mv	Medellín, Colombia
institution	Universidad de Antioquia
bitstream.url.fl_str_mv	http://bibliotecadigital.udea.edu.co/bitstream/10495/10170/1/CalleOsornoJaime_2017_DeteccionEnzimasCepas.pdf http://bibliotecadigital.udea.edu.co/bitstream/10495/10170/2/license_url http://bibliotecadigital.udea.edu.co/bitstream/10495/10170/3/license_text http://bibliotecadigital.udea.edu.co/bitstream/10495/10170/4/license_rdf http://bibliotecadigital.udea.edu.co/bitstream/10495/10170/5/license.txt
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spelling	Gato Cárdenas, YohanaMárquez Gutiérrez, María ElenaBaró Robaina, YamiléCalle Osorno, Jaime de Jesús2018-10-02T22:01:39Z2018-10-02T22:01:39Z2017Gato Y, Márquez M, Baró Y, Calle J. Detección de enzimas extracelulares en cepas cubanas del complejo Metarhizium anisopliae con acción entomopatogénica contra Cylas formicarius Fabricius (Coleoptera: Brentidae). Actual Bio. 2017; 39(106):71-78. DOI: 10.17533/udea.acbi.v39n106a070304-3584http://hdl.handle.net/10495/1017010.17533/udea.acbi.v39n106a072145-7166ABSTRACT: This study reports the detection, by biochemical methods, of extracellular enzymes related to the biological activity of nine strains of the complex Metarhizium anisopliae (Metsch.) Sorokin. We determined the pathogenic capacity against the insect Cylas formicarius Fabricius at different concentrations, calculated the lethal time and concentration, and analyzed both the presence of extracellular enzymes in the fungal strains as well as their relation to the entomopathogenic effect and virulence against C. formicarius. All strains of the M. anisopliae complex studied were pathogenic with an enzymatic activity index greater than 1. The LBM-30 strain was the most effective with presence of protease, chitinase, caseinase, amylase and lipase enzymes. This result corroborates the relationship between of the enzymatic activity with the observed virulence.RESUMEN: El presente trabajo informa la detección, por métodos bioquímicos, de enzimas extracelulares relacionadas con la actividad biológica de nueve cepas del complejo Metarhizium anisopliae (Metsch.) Sorokin. Se determinó, a diferentes concentraciones, la capacidad patogénica contra el insecto Cylas formicarius Fabricius, y se calculó el tiempo y la concentración letal. Se analizó tanto la presencia de enzimas extracelulares en las cepas fúngicas como su relación con el efecto entomopatogénico y virulencia contra C. formicarius. Todas las cepas del complejo M. anisopliae estudiadas resultaron patogénicas exhibiendo un índice de actividad enzimática superior a 1. La cepa LBM-30 fue la más e ciente al detectarse la presencia de enzimas proteasa, quitinasa, caseínasa, amilasa y lipasa. Esto corrobora la relación de la actividad enzimática con la virulencia observada.COL0039509application/pdfspaUniversidad de Antioquia, Facultad de Ciencias Exactas y Naturales, Instituto de BiologíaBiocontrol y Microbiología Ambiental -BIOMA-Medellín, Colombiainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_2df8fbb1https://purl.org/redcol/resource_type/ARTArtículo de investigaciónhttp://purl.org/coar/version/c_970fb48d4fbd8a85Atribución-NoComercial-CompartirIgual 2.5 Colombia (CC BY-NC-SA 2.5 CO)info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/co/http://purl.org/coar/access_right/c_abf2https://creativecommons.org/licenses/by-nc-sa/4.0/Control biológicoBiocontrolHongosHongos de suelosHongos entomopatógenosEntomopathogenic fungiFungiPathogenic activitySoil fungiDetección de enzimas extracelulares en cepas cubanas del complejo Metarhizium anisopliae con acción entomopatogénica contra Cylas formicarius Fabricius (Coleoptera: Brentidae)Detection of extracellular enzymes in cuban strains of Metarhizium anisopliae complex with entomopathogenic action against Cylas formicarius Fabricius (Coleoptera: Brentidae)Actual. Biol.Actualidades Biológicas787139106ORIGINALCalleOsornoJaime_2017_DeteccionEnzimasCepas.pdfCalleOsornoJaime_2017_DeteccionEnzimasCepas.pdfArtículo de investigaciónapplication/pdf1916451http://bibliotecadigital.udea.edu.co/bitstream/10495/10170/1/CalleOsornoJaime_2017_DeteccionEnzimasCepas.pdf982b354c7e6ee02ffb22168db6eccb49MD51CC-LICENSElicense_urllicense_urltext/plain; charset=utf-849http://bibliotecadigital.udea.edu.co/bitstream/10495/10170/2/license_url4afdbb8c545fd630ea7db775da747b2fMD52license_textlicense_texttext/html; charset=utf-80http://bibliotecadigital.udea.edu.co/bitstream/10495/10170/3/license_textd41d8cd98f00b204e9800998ecf8427eMD53license_rdflicense_rdfLicenciaapplication/rdf+xml; charset=utf-80http://bibliotecadigital.udea.edu.co/bitstream/10495/10170/4/license_rdfd41d8cd98f00b204e9800998ecf8427eMD54LICENSElicense.txtlicense.txttext/plain; charset=utf-81748http://bibliotecadigital.udea.edu.co/bitstream/10495/10170/5/license.txt8a4605be74aa9ea9d79846c1fba20a33MD5510495/10170oai:bibliotecadigital.udea.edu.co:10495/101702021-07-23 22:04:49.448Repositorio Institucional Universidad de Antioquiaandres.perez@udea.edu.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

Detección de enzimas extracelulares en cepas cubanas del complejo Metarhizium anisopliae con acción entomopatogénica contra Cylas formicarius Fabricius (Coleoptera: Brentidae)

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