Agrobacterium tumefaciens-mediated transformation of Paracoccidioides brasiliensis
ABSTRACT: Paracoccidioides brasiliensis is the causative agent of paracoccidioidomycosis, an important mycosis endemic to Latin America. As the tools to study gene function in P. brasiliensis are only in the early stage of development, there is presently no system that allows for both the delivery a...
- Autores:
-
Leal Torres, Claudia Victoria
Montes Vergara, Beatriz Alicia
Mesa Arango, Ana Cecilia
Rúa Giraldo, Álvaro León
Corredor Rodríguez, Mauricio
Restrepo Moreno, Ángela
McEwen Ochoa, Juan Guillermo
- Tipo de recurso:
- Article of investigation
- Fecha de publicación:
- 2004
- Institución:
- Universidad de Antioquia
- Repositorio:
- Repositorio UdeA
- Idioma:
- eng
- OAI Identifier:
- oai:bibliotecadigital.udea.edu.co:10495/24134
- Acceso en línea:
- http://hdl.handle.net/10495/24134
- Palabra clave:
- Paracoccidioides
Agrobacterium tumefaciens
- Rights
- openAccess
- License
- http://creativecommons.org/licenses/by-nc/2.5/co/
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Agrobacterium tumefaciens-mediated transformation of Paracoccidioides brasiliensis |
| title |
Agrobacterium tumefaciens-mediated transformation of Paracoccidioides brasiliensis |
| spellingShingle |
Agrobacterium tumefaciens-mediated transformation of Paracoccidioides brasiliensis Paracoccidioides Agrobacterium tumefaciens |
| title_short |
Agrobacterium tumefaciens-mediated transformation of Paracoccidioides brasiliensis |
| title_full |
Agrobacterium tumefaciens-mediated transformation of Paracoccidioides brasiliensis |
| title_fullStr |
Agrobacterium tumefaciens-mediated transformation of Paracoccidioides brasiliensis |
| title_full_unstemmed |
Agrobacterium tumefaciens-mediated transformation of Paracoccidioides brasiliensis |
| title_sort |
Agrobacterium tumefaciens-mediated transformation of Paracoccidioides brasiliensis |
| dc.creator.fl_str_mv |
Leal Torres, Claudia Victoria Montes Vergara, Beatriz Alicia Mesa Arango, Ana Cecilia Rúa Giraldo, Álvaro León Corredor Rodríguez, Mauricio Restrepo Moreno, Ángela McEwen Ochoa, Juan Guillermo |
| dc.contributor.author.none.fl_str_mv |
Leal Torres, Claudia Victoria Montes Vergara, Beatriz Alicia Mesa Arango, Ana Cecilia Rúa Giraldo, Álvaro León Corredor Rodríguez, Mauricio Restrepo Moreno, Ángela McEwen Ochoa, Juan Guillermo |
| dc.subject.decs.none.fl_str_mv |
Paracoccidioides Agrobacterium tumefaciens |
| topic |
Paracoccidioides Agrobacterium tumefaciens |
| description |
ABSTRACT: Paracoccidioides brasiliensis is the causative agent of paracoccidioidomycosis, an important mycosis endemic to Latin America. As the tools to study gene function in P. brasiliensis are only in the early stage of development, there is presently no system that allows for both the delivery and integration of exogenous nucleic acids into its genome. We report in this paper the transformation of the yeast phase of P. brasiliensis (ATCC-60855) with Agrobacterium tumefaciens (GV3101) carrying the vector pAD1625. The microorganisms were co-cultivated for 2 days and then incubated for 10 days at 358C on selective media. PCR and dot-blot targeted at a fragment of 222 bp from the hph (hygromycin phosphotransferase) gene which confers Hygr confirmed the transformation of P. brasiliensis. |
| publishDate |
2004 |
| dc.date.issued.none.fl_str_mv |
2004 |
| dc.date.accessioned.none.fl_str_mv |
2021-11-16T00:06:24Z |
| dc.date.available.none.fl_str_mv |
2021-11-16T00:06:24Z |
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info:eu-repo/semantics/article |
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http://purl.org/coar/version/c_970fb48d4fbd8a85 |
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http://purl.org/coar/resource_type/c_2df8fbb1 |
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Artículo de investigación |
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http://purl.org/coar/resource_type/c_2df8fbb1 |
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publishedVersion |
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1369-3786 1460-2709 |
| dc.identifier.uri.none.fl_str_mv |
http://hdl.handle.net/10495/24134 |
| dc.identifier.doi.none.fl_str_mv |
10.1080/13693780410001712007 |
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1369-3786 1460-2709 10.1080/13693780410001712007 |
| url |
http://hdl.handle.net/10495/24134 |
| dc.language.iso.spa.fl_str_mv |
eng |
| language |
eng |
| dc.relation.ispartofjournalabbrev.spa.fl_str_mv |
Med. Mycol. |
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info:eu-repo/semantics/openAccess |
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http://creativecommons.org/licenses/by-nc/2.5/co/ |
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openAccess |
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http://creativecommons.org/licenses/by-nc/2.5/co/ http://purl.org/coar/access_right/c_abf2 https://creativecommons.org/licenses/by-nc/4.0/ |
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5 |
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application/pdf |
| dc.publisher.spa.fl_str_mv |
Oxford University Press |
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Biología Celular y Molecular CIB U. de A. U. del Rosario Micología Médica y Experimental GEBIOMIC (Genética y Bioquímica de Microorganismos) |
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Oxford, Inglaterra |
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Universidad de Antioquia |
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Leal Torres, Claudia VictoriaMontes Vergara, Beatriz AliciaMesa Arango, Ana CeciliaRúa Giraldo, Álvaro LeónCorredor Rodríguez, MauricioRestrepo Moreno, ÁngelaMcEwen Ochoa, Juan Guillermo2021-11-16T00:06:24Z2021-11-16T00:06:24Z20041369-37861460-2709http://hdl.handle.net/10495/2413410.1080/13693780410001712007ABSTRACT: Paracoccidioides brasiliensis is the causative agent of paracoccidioidomycosis, an important mycosis endemic to Latin America. As the tools to study gene function in P. brasiliensis are only in the early stage of development, there is presently no system that allows for both the delivery and integration of exogenous nucleic acids into its genome. We report in this paper the transformation of the yeast phase of P. brasiliensis (ATCC-60855) with Agrobacterium tumefaciens (GV3101) carrying the vector pAD1625. The microorganisms were co-cultivated for 2 days and then incubated for 10 days at 358C on selective media. PCR and dot-blot targeted at a fragment of 222 bp from the hph (hygromycin phosphotransferase) gene which confers Hygr confirmed the transformation of P. brasiliensis.COL0013709COL0000962COL00140645application/pdfengOxford University PressBiología Celular y Molecular CIB U. de A. U. del RosarioMicología Médica y ExperimentalGEBIOMIC (Genética y Bioquímica de Microorganismos)Oxford, Inglaterrainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_2df8fbb1https://purl.org/redcol/resource_type/ARTArtículo de investigaciónhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc/2.5/co/http://purl.org/coar/access_right/c_abf2https://creativecommons.org/licenses/by-nc/4.0/Agrobacterium tumefaciens-mediated transformation of Paracoccidioides brasiliensisParacoccidioidesAgrobacterium tumefaciensMed. Mycol.Medical Mycology391395424CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8933http://bibliotecadigital.udea.edu.co/bitstream/10495/24134/2/license_rdfc0c92b0ffc8b7d22d9cf56754a416a76MD52LICENSElicense.txtlicense.txttext/plain; charset=utf-81748http://bibliotecadigital.udea.edu.co/bitstream/10495/24134/3/license.txt8a4605be74aa9ea9d79846c1fba20a33MD53ORIGINALRestrepoAngela_2004_AgrobacteriumTransformation.pdfRestrepoAngela_2004_AgrobacteriumTransformation.pdfArtículo de investigaciónapplication/pdf185320http://bibliotecadigital.udea.edu.co/bitstream/10495/24134/1/RestrepoAngela_2004_AgrobacteriumTransformation.pdfad57bebaaed45fb0757423dad6c5edb2MD5110495/24134oai:bibliotecadigital.udea.edu.co:10495/241342022-04-22 10:22:14.501Repositorio Institucional Universidad de Antioquiaandres.perez@udea.edu.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 |