Simulating gas-liquid mass transfer in a spin filter bioreactor

RESUMEN: Computational fluid dynamics (CFD) and population balance model (PBM) model have been used to simulate hydrodynamics and mass transfer in a 0.014 m3 Spin Filter Bioreactor. The operating conditions chosen were defined by typical settings used for culturing plant cells. Turbulence, rotating...

Full description

Autores:
Gelves Zambrano, Germán Ricardo
Tipo de recurso:
Article of investigation
Fecha de publicación:
2015
Institución:
Universidad de Antioquia
Repositorio:
Repositorio UdeA
Idioma:
eng
OAI Identifier:
oai:bibliotecadigital.udea.edu.co:10495/5988
Acceso en línea:
http://hdl.handle.net/10495/5988
Palabra clave:
Transferencia de masa
Bioreactores
Dinámica de fluidos
Hidrodinámica
Procesos biológicos
Escalado de procesos
Rights
openAccess
License
Atribución-NoComercial-CompartirIgual 2.5 Colombia (CC BY-NC-SA 2.5 CO)
id UDEA2_b172695fcacf59f4ed0df6b73f69da23
oai_identifier_str oai:bibliotecadigital.udea.edu.co:10495/5988
network_acronym_str UDEA2
network_name_str Repositorio UdeA
repository_id_str
dc.title.spa.fl_str_mv Simulating gas-liquid mass transfer in a spin filter bioreactor
dc.title.alternative.spa.fl_str_mv Simulación de la transferencia de masa en un biorreactor de perfusión operado con un filtro rotativo
title Simulating gas-liquid mass transfer in a spin filter bioreactor
spellingShingle Simulating gas-liquid mass transfer in a spin filter bioreactor
Transferencia de masa
Bioreactores
Dinámica de fluidos
Hidrodinámica
Procesos biológicos
Escalado de procesos
title_short Simulating gas-liquid mass transfer in a spin filter bioreactor
title_full Simulating gas-liquid mass transfer in a spin filter bioreactor
title_fullStr Simulating gas-liquid mass transfer in a spin filter bioreactor
title_full_unstemmed Simulating gas-liquid mass transfer in a spin filter bioreactor
title_sort Simulating gas-liquid mass transfer in a spin filter bioreactor
dc.creator.fl_str_mv Gelves Zambrano, Germán Ricardo
dc.contributor.author.none.fl_str_mv Gelves Zambrano, Germán Ricardo
dc.subject.none.fl_str_mv Transferencia de masa
Bioreactores
Dinámica de fluidos
Hidrodinámica
Procesos biológicos
Escalado de procesos
topic Transferencia de masa
Bioreactores
Dinámica de fluidos
Hidrodinámica
Procesos biológicos
Escalado de procesos
description RESUMEN: Computational fluid dynamics (CFD) and population balance model (PBM) model have been used to simulate hydrodynamics and mass transfer in a 0.014 m3 Spin Filter Bioreactor. The operating conditions chosen were defined by typical settings used for culturing plant cells. Turbulence, rotating flow, bubbles breakage and coalescence were simulated by using the k-e, MRF (Multiple Reference Frame) and PBM approaches, respectively. The numerical results from different operational conditions are compared with experimental data obtained from measurements and good fitting data is achieved. Interested by these simulated and experimental results CFD simulations are qualified as a very promising tool not only for predicting gas-liquid hydrodynamics but also for finding design requirements that must be implemented to optimize an aerobic bioprocessing useful for plant cell culture applications which are characterized by the constrain of achieving relatively high mass transfer conditions and avoiding cellular damage due to hydrodynamic conditions.
publishDate 2015
dc.date.issued.none.fl_str_mv 2015
dc.date.accessioned.none.fl_str_mv 2017-01-10T17:13:10Z
dc.date.available.none.fl_str_mv 2017-01-10T17:13:10Z
dc.type.spa.fl_str_mv info:eu-repo/semantics/article
dc.type.coarversion.fl_str_mv http://purl.org/coar/version/c_970fb48d4fbd8a86
dc.type.coar.spa.fl_str_mv http://purl.org/coar/resource_type/c_2df8fbb1
dc.type.redcol.spa.fl_str_mv https://purl.org/redcol/resource_type/ART
dc.type.local.spa.fl_str_mv Artículo de investigación
format http://purl.org/coar/resource_type/c_2df8fbb1
dc.identifier.citation.spa.fl_str_mv L. C. Niño and G. R. Gelves, "Simulating gas-liquid mass transfer in a spin filter bioreactor", Rev. Fac. Ing. Univ. Antioquia, no. 75, pp. 163-174, 2015.
dc.identifier.issn.none.fl_str_mv 0120-6230
dc.identifier.uri.none.fl_str_mv http://hdl.handle.net/10495/5988
dc.identifier.doi.none.fl_str_mv 10.17533/udea.redin.n75a16
dc.identifier.eissn.none.fl_str_mv 2422-2844
identifier_str_mv L. C. Niño and G. R. Gelves, "Simulating gas-liquid mass transfer in a spin filter bioreactor", Rev. Fac. Ing. Univ. Antioquia, no. 75, pp. 163-174, 2015.
0120-6230
10.17533/udea.redin.n75a16
2422-2844
url http://hdl.handle.net/10495/5988
dc.language.iso.spa.fl_str_mv eng
language eng
dc.relation.ispartofjournalabbrev.spa.fl_str_mv Rev. Fac. Ing. Univ. Antioquia
dc.rights.*.fl_str_mv Atribución-NoComercial-CompartirIgual 2.5 Colombia (CC BY-NC-SA 2.5 CO)
dc.rights.spa.fl_str_mv info:eu-repo/semantics/openAccess
dc.rights.uri.*.fl_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/co/
dc.rights.accessrights.spa.fl_str_mv http://purl.org/coar/access_right/c_abf2
dc.rights.creativecommons.spa.fl_str_mv https://creativecommons.org/licenses/by-nc-sa/4.0/
rights_invalid_str_mv Atribución-NoComercial-CompartirIgual 2.5 Colombia (CC BY-NC-SA 2.5 CO)
https://creativecommons.org/licenses/by-nc-sa/2.5/co/
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by-nc-sa/4.0/
eu_rights_str_mv openAccess
dc.format.extent.spa.fl_str_mv 11
dc.format.mimetype.spa.fl_str_mv application/pdf
dc.publisher.spa.fl_str_mv Universidad de Antioquia, Facultad de Ingeniería
dc.publisher.group.spa.fl_str_mv Bioprocesos
dc.publisher.place.spa.fl_str_mv Medellín, Colombia
institution Universidad de Antioquia
bitstream.url.fl_str_mv http://bibliotecadigital.udea.edu.co/bitstream/10495/5988/1/Ni%c3%b1oLilibeth_2015_SimulatingGasLiquid.pdf
http://bibliotecadigital.udea.edu.co/bitstream/10495/5988/2/license_url
http://bibliotecadigital.udea.edu.co/bitstream/10495/5988/3/license_text
http://bibliotecadigital.udea.edu.co/bitstream/10495/5988/4/license_rdf
http://bibliotecadigital.udea.edu.co/bitstream/10495/5988/5/license.txt
bitstream.checksum.fl_str_mv 6baeed17bca371c98cee1c9a973f4af9
4afdbb8c545fd630ea7db775da747b2f
d41d8cd98f00b204e9800998ecf8427e
d41d8cd98f00b204e9800998ecf8427e
8a4605be74aa9ea9d79846c1fba20a33
bitstream.checksumAlgorithm.fl_str_mv MD5
MD5
MD5
MD5
MD5
repository.name.fl_str_mv Repositorio Institucional Universidad de Antioquia
repository.mail.fl_str_mv andres.perez@udea.edu.co
_version_ 1805390177360674816
spelling Gelves Zambrano, Germán Ricardo2017-01-10T17:13:10Z2017-01-10T17:13:10Z2015L. C. Niño and G. R. Gelves, "Simulating gas-liquid mass transfer in a spin filter bioreactor", Rev. Fac. Ing. Univ. Antioquia, no. 75, pp. 163-174, 2015.0120-6230http://hdl.handle.net/10495/598810.17533/udea.redin.n75a162422-2844RESUMEN: Computational fluid dynamics (CFD) and population balance model (PBM) model have been used to simulate hydrodynamics and mass transfer in a 0.014 m3 Spin Filter Bioreactor. The operating conditions chosen were defined by typical settings used for culturing plant cells. Turbulence, rotating flow, bubbles breakage and coalescence were simulated by using the k-e, MRF (Multiple Reference Frame) and PBM approaches, respectively. The numerical results from different operational conditions are compared with experimental data obtained from measurements and good fitting data is achieved. Interested by these simulated and experimental results CFD simulations are qualified as a very promising tool not only for predicting gas-liquid hydrodynamics but also for finding design requirements that must be implemented to optimize an aerobic bioprocessing useful for plant cell culture applications which are characterized by the constrain of achieving relatively high mass transfer conditions and avoiding cellular damage due to hydrodynamic conditions.ABSTRACT: Mediante dinámica de fluidos computacional (CFD) y métodos de balance poblacional (PBM) se simuló la hidrodinámica líquido-gaseosa y la transferencia de masa en un biorreactor de 0,014 m3 operado con un Spin Filter para cultivos en modo perfusión. Las condiciones de operación fueron definidas con base en los requerimientos para células vegetales en suspensión. Los fenómenos de turbulencia, flujo giratorio, ruptura y coalescencia de burbujas fueron simulados utilizando los modelos k-e, MRF (Multiple Reference Frame) y PBM. Se logra una predicción aceptable mediante la comparación entre los resultados numéricos de las diferentes condiciones de operación y los datos experimentales de los valores del coeficiente de transferencia de masa Con la motivación de estos resultados simulados y validados experimentalmente, se observa que CFD puede ser una herramienta muy prometedora, no sólo para la predicción de la hidrodinámica líquido-gaseosa, sino también para encontrar los requisitos de diseño que se deben implementar para optimizar un proceso biológico aerobio útil para aplicaciones de cultivos celulares de plantas, que son comúnmente caracterizados por el requerimiento de mantener condiciones relativamente altas tasa de transferencia de masa y simultáneamente evitar el daño celular debido a las condiciones hidrodinámicas.11application/pdfengUniversidad de Antioquia, Facultad de IngenieríaBioprocesosMedellín, Colombiainfo:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_2df8fbb1https://purl.org/redcol/resource_type/ARTArtículo de investigaciónhttp://purl.org/coar/version/c_970fb48d4fbd8a86Atribución-NoComercial-CompartirIgual 2.5 Colombia (CC BY-NC-SA 2.5 CO)info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/co/http://purl.org/coar/access_right/c_abf2https://creativecommons.org/licenses/by-nc-sa/4.0/Transferencia de masaBioreactoresDinámica de fluidosHidrodinámicaProcesos biológicosEscalado de procesosSimulating gas-liquid mass transfer in a spin filter bioreactorSimulación de la transferencia de masa en un biorreactor de perfusión operado con un filtro rotativoRev. Fac. Ing. Univ. AntioquiaRevista Facultad de Ingeniería Universidad de Antioquia16317475ORIGINALNiñoLilibeth_2015_SimulatingGasLiquid.pdfNiñoLilibeth_2015_SimulatingGasLiquid.pdfArtículo de investigaciónapplication/pdf3377468http://bibliotecadigital.udea.edu.co/bitstream/10495/5988/1/Ni%c3%b1oLilibeth_2015_SimulatingGasLiquid.pdf6baeed17bca371c98cee1c9a973f4af9MD51CC-LICENSElicense_urllicense_urltext/plain; charset=utf-849http://bibliotecadigital.udea.edu.co/bitstream/10495/5988/2/license_url4afdbb8c545fd630ea7db775da747b2fMD52license_textlicense_texttext/html; charset=utf-80http://bibliotecadigital.udea.edu.co/bitstream/10495/5988/3/license_textd41d8cd98f00b204e9800998ecf8427eMD53license_rdflicense_rdfapplication/rdf+xml; charset=utf-80http://bibliotecadigital.udea.edu.co/bitstream/10495/5988/4/license_rdfd41d8cd98f00b204e9800998ecf8427eMD54LICENSElicense.txtlicense.txttext/plain; charset=utf-81748http://bibliotecadigital.udea.edu.co/bitstream/10495/5988/5/license.txt8a4605be74aa9ea9d79846c1fba20a33MD5510495/5988oai:bibliotecadigital.udea.edu.co:10495/59882021-05-07 11:45:23.568Repositorio Institucional Universidad de Antioquiaandres.perez@udea.edu.coTk9URTogUExBQ0UgWU9VUiBPV04gTElDRU5TRSBIRVJFClRoaXMgc2FtcGxlIGxpY2Vuc2UgaXMgcHJvdmlkZWQgZm9yIGluZm9ybWF0aW9uYWwgcHVycG9zZXMgb25seS4KCk5PTi1FWENMVVNJVkUgRElTVFJJQlVUSU9OIExJQ0VOU0UKCkJ5IHNpZ25pbmcgYW5kIHN1Ym1pdHRpbmcgdGhpcyBsaWNlbnNlLCB5b3UgKHRoZSBhdXRob3Iocykgb3IgY29weXJpZ2h0Cm93bmVyKSBncmFudHMgdG8gRFNwYWNlIFVuaXZlcnNpdHkgKERTVSkgdGhlIG5vbi1leGNsdXNpdmUgcmlnaHQgdG8gcmVwcm9kdWNlLAp0cmFuc2xhdGUgKGFzIGRlZmluZWQgYmVsb3cpLCBhbmQvb3IgZGlzdHJpYnV0ZSB5b3VyIHN1Ym1pc3Npb24gKGluY2x1ZGluZwp0aGUgYWJzdHJhY3QpIHdvcmxkd2lkZSBpbiBwcmludCBhbmQgZWxlY3Ryb25pYyBmb3JtYXQgYW5kIGluIGFueSBtZWRpdW0sCmluY2x1ZGluZyBidXQgbm90IGxpbWl0ZWQgdG8gYXVkaW8gb3IgdmlkZW8uCgpZb3UgYWdyZWUgdGhhdCBEU1UgbWF5LCB3aXRob3V0IGNoYW5naW5nIHRoZSBjb250ZW50LCB0cmFuc2xhdGUgdGhlCnN1Ym1pc3Npb24gdG8gYW55IG1lZGl1bSBvciBmb3JtYXQgZm9yIHRoZSBwdXJwb3NlIG9mIHByZXNlcnZhdGlvbi4KCllvdSBhbHNvIGFncmVlIHRoYXQgRFNVIG1heSBrZWVwIG1vcmUgdGhhbiBvbmUgY29weSBvZiB0aGlzIHN1Ym1pc3Npb24gZm9yCnB1cnBvc2VzIG9mIHNlY3VyaXR5LCBiYWNrLXVwIGFuZCBwcmVzZXJ2YXRpb24uCgpZb3UgcmVwcmVzZW50IHRoYXQgdGhlIHN1Ym1pc3Npb24gaXMgeW91ciBvcmlnaW5hbCB3b3JrLCBhbmQgdGhhdCB5b3UgaGF2ZQp0aGUgcmlnaHQgdG8gZ3JhbnQgdGhlIHJpZ2h0cyBjb250YWluZWQgaW4gdGhpcyBsaWNlbnNlLiBZb3UgYWxzbyByZXByZXNlbnQKdGhhdCB5b3VyIHN1Ym1pc3Npb24gZG9lcyBub3QsIHRvIHRoZSBiZXN0IG9mIHlvdXIga25vd2xlZGdlLCBpbmZyaW5nZSB1cG9uCmFueW9uZSdzIGNvcHlyaWdodC4KCklmIHRoZSBzdWJtaXNzaW9uIGNvbnRhaW5zIG1hdGVyaWFsIGZvciB3aGljaCB5b3UgZG8gbm90IGhvbGQgY29weXJpZ2h0LAp5b3UgcmVwcmVzZW50IHRoYXQgeW91IGhhdmUgb2J0YWluZWQgdGhlIHVucmVzdHJpY3RlZCBwZXJtaXNzaW9uIG9mIHRoZQpjb3B5cmlnaHQgb3duZXIgdG8gZ3JhbnQgRFNVIHRoZSByaWdodHMgcmVxdWlyZWQgYnkgdGhpcyBsaWNlbnNlLCBhbmQgdGhhdApzdWNoIHRoaXJkLXBhcnR5IG93bmVkIG1hdGVyaWFsIGlzIGNsZWFybHkgaWRlbnRpZmllZCBhbmQgYWNrbm93bGVkZ2VkCndpdGhpbiB0aGUgdGV4dCBvciBjb250ZW50IG9mIHRoZSBzdWJtaXNzaW9uLgoKSUYgVEhFIFNVQk1JU1NJT04gSVMgQkFTRUQgVVBPTiBXT1JLIFRIQVQgSEFTIEJFRU4gU1BPTlNPUkVEIE9SIFNVUFBPUlRFRApCWSBBTiBBR0VOQ1kgT1IgT1JHQU5JWkFUSU9OIE9USEVSIFRIQU4gRFNVLCBZT1UgUkVQUkVTRU5UIFRIQVQgWU9VIEhBVkUKRlVMRklMTEVEIEFOWSBSSUdIVCBPRiBSRVZJRVcgT1IgT1RIRVIgT0JMSUdBVElPTlMgUkVRVUlSRUQgQlkgU1VDSApDT05UUkFDVCBPUiBBR1JFRU1FTlQuCgpEU1Ugd2lsbCBjbGVhcmx5IGlkZW50aWZ5IHlvdXIgbmFtZShzKSBhcyB0aGUgYXV0aG9yKHMpIG9yIG93bmVyKHMpIG9mIHRoZQpzdWJtaXNzaW9uLCBhbmQgd2lsbCBub3QgbWFrZSBhbnkgYWx0ZXJhdGlvbiwgb3RoZXIgdGhhbiBhcyBhbGxvd2VkIGJ5IHRoaXMKbGljZW5zZSwgdG8geW91ciBzdWJtaXNzaW9uLgo=

Publicaciones similares