Simulating gas-liquid mass transfer in a spin filter bioreactor
RESUMEN: Computational fluid dynamics (CFD) and population balance model (PBM) model have been used to simulate hydrodynamics and mass transfer in a 0.014 m3 Spin Filter Bioreactor. The operating conditions chosen were defined by typical settings used for culturing plant cells. Turbulence, rotating...
- Autores:
-
Gelves Zambrano, Germán Ricardo
- Tipo de recurso:
- Article of investigation
- Fecha de publicación:
- 2015
- Institución:
- Universidad de Antioquia
- Repositorio:
- Repositorio UdeA
- Idioma:
- eng
- OAI Identifier:
- oai:bibliotecadigital.udea.edu.co:10495/5988
- Acceso en línea:
- http://hdl.handle.net/10495/5988
- Palabra clave:
- Transferencia de masa
Bioreactores
Dinámica de fluidos
Hidrodinámica
Procesos biológicos
Escalado de procesos
- Rights
- openAccess
- License
- Atribución-NoComercial-CompartirIgual 2.5 Colombia (CC BY-NC-SA 2.5 CO)
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| dc.title.spa.fl_str_mv |
Simulating gas-liquid mass transfer in a spin filter bioreactor |
| dc.title.alternative.spa.fl_str_mv |
Simulación de la transferencia de masa en un biorreactor de perfusión operado con un filtro rotativo |
| title |
Simulating gas-liquid mass transfer in a spin filter bioreactor |
| spellingShingle |
Simulating gas-liquid mass transfer in a spin filter bioreactor Transferencia de masa Bioreactores Dinámica de fluidos Hidrodinámica Procesos biológicos Escalado de procesos |
| title_short |
Simulating gas-liquid mass transfer in a spin filter bioreactor |
| title_full |
Simulating gas-liquid mass transfer in a spin filter bioreactor |
| title_fullStr |
Simulating gas-liquid mass transfer in a spin filter bioreactor |
| title_full_unstemmed |
Simulating gas-liquid mass transfer in a spin filter bioreactor |
| title_sort |
Simulating gas-liquid mass transfer in a spin filter bioreactor |
| dc.creator.fl_str_mv |
Gelves Zambrano, Germán Ricardo |
| dc.contributor.author.none.fl_str_mv |
Gelves Zambrano, Germán Ricardo |
| dc.subject.none.fl_str_mv |
Transferencia de masa Bioreactores Dinámica de fluidos Hidrodinámica Procesos biológicos Escalado de procesos |
| topic |
Transferencia de masa Bioreactores Dinámica de fluidos Hidrodinámica Procesos biológicos Escalado de procesos |
| description |
RESUMEN: Computational fluid dynamics (CFD) and population balance model (PBM) model have been used to simulate hydrodynamics and mass transfer in a 0.014 m3 Spin Filter Bioreactor. The operating conditions chosen were defined by typical settings used for culturing plant cells. Turbulence, rotating flow, bubbles breakage and coalescence were simulated by using the k-e, MRF (Multiple Reference Frame) and PBM approaches, respectively. The numerical results from different operational conditions are compared with experimental data obtained from measurements and good fitting data is achieved. Interested by these simulated and experimental results CFD simulations are qualified as a very promising tool not only for predicting gas-liquid hydrodynamics but also for finding design requirements that must be implemented to optimize an aerobic bioprocessing useful for plant cell culture applications which are characterized by the constrain of achieving relatively high mass transfer conditions and avoiding cellular damage due to hydrodynamic conditions. |
| publishDate |
2015 |
| dc.date.issued.none.fl_str_mv |
2015 |
| dc.date.accessioned.none.fl_str_mv |
2017-01-10T17:13:10Z |
| dc.date.available.none.fl_str_mv |
2017-01-10T17:13:10Z |
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info:eu-repo/semantics/article |
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http://purl.org/coar/version/c_970fb48d4fbd8a86 |
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http://purl.org/coar/resource_type/c_2df8fbb1 |
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https://purl.org/redcol/resource_type/ART |
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Artículo de investigación |
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http://purl.org/coar/resource_type/c_2df8fbb1 |
| dc.identifier.citation.spa.fl_str_mv |
L. C. Niño and G. R. Gelves, "Simulating gas-liquid mass transfer in a spin filter bioreactor", Rev. Fac. Ing. Univ. Antioquia, no. 75, pp. 163-174, 2015. |
| dc.identifier.issn.none.fl_str_mv |
0120-6230 |
| dc.identifier.uri.none.fl_str_mv |
http://hdl.handle.net/10495/5988 |
| dc.identifier.doi.none.fl_str_mv |
10.17533/udea.redin.n75a16 |
| dc.identifier.eissn.none.fl_str_mv |
2422-2844 |
| identifier_str_mv |
L. C. Niño and G. R. Gelves, "Simulating gas-liquid mass transfer in a spin filter bioreactor", Rev. Fac. Ing. Univ. Antioquia, no. 75, pp. 163-174, 2015. 0120-6230 10.17533/udea.redin.n75a16 2422-2844 |
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http://hdl.handle.net/10495/5988 |
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eng |
| language |
eng |
| dc.relation.ispartofjournalabbrev.spa.fl_str_mv |
Rev. Fac. Ing. Univ. Antioquia |
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Atribución-NoComercial-CompartirIgual 2.5 Colombia (CC BY-NC-SA 2.5 CO) |
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info:eu-repo/semantics/openAccess |
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https://creativecommons.org/licenses/by-nc-sa/2.5/co/ |
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Atribución-NoComercial-CompartirIgual 2.5 Colombia (CC BY-NC-SA 2.5 CO) https://creativecommons.org/licenses/by-nc-sa/2.5/co/ http://purl.org/coar/access_right/c_abf2 https://creativecommons.org/licenses/by-nc-sa/4.0/ |
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openAccess |
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11 |
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application/pdf |
| dc.publisher.spa.fl_str_mv |
Universidad de Antioquia, Facultad de Ingeniería |
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Bioprocesos |
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Medellín, Colombia |
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Universidad de Antioquia |
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Gelves Zambrano, Germán Ricardo2017-01-10T17:13:10Z2017-01-10T17:13:10Z2015L. C. Niño and G. R. Gelves, "Simulating gas-liquid mass transfer in a spin filter bioreactor", Rev. Fac. Ing. Univ. Antioquia, no. 75, pp. 163-174, 2015.0120-6230http://hdl.handle.net/10495/598810.17533/udea.redin.n75a162422-2844RESUMEN: Computational fluid dynamics (CFD) and population balance model (PBM) model have been used to simulate hydrodynamics and mass transfer in a 0.014 m3 Spin Filter Bioreactor. The operating conditions chosen were defined by typical settings used for culturing plant cells. Turbulence, rotating flow, bubbles breakage and coalescence were simulated by using the k-e, MRF (Multiple Reference Frame) and PBM approaches, respectively. The numerical results from different operational conditions are compared with experimental data obtained from measurements and good fitting data is achieved. Interested by these simulated and experimental results CFD simulations are qualified as a very promising tool not only for predicting gas-liquid hydrodynamics but also for finding design requirements that must be implemented to optimize an aerobic bioprocessing useful for plant cell culture applications which are characterized by the constrain of achieving relatively high mass transfer conditions and avoiding cellular damage due to hydrodynamic conditions.ABSTRACT: Mediante dinámica de fluidos computacional (CFD) y métodos de balance poblacional (PBM) se simuló la hidrodinámica líquido-gaseosa y la transferencia de masa en un biorreactor de 0,014 m3 operado con un Spin Filter para cultivos en modo perfusión. Las condiciones de operación fueron definidas con base en los requerimientos para células vegetales en suspensión. Los fenómenos de turbulencia, flujo giratorio, ruptura y coalescencia de burbujas fueron simulados utilizando los modelos k-e, MRF (Multiple Reference Frame) y PBM. Se logra una predicción aceptable mediante la comparación entre los resultados numéricos de las diferentes condiciones de operación y los datos experimentales de los valores del coeficiente de transferencia de masa Con la motivación de estos resultados simulados y validados experimentalmente, se observa que CFD puede ser una herramienta muy prometedora, no sólo para la predicción de la hidrodinámica líquido-gaseosa, sino también para encontrar los requisitos de diseño que se deben implementar para optimizar un proceso biológico aerobio útil para aplicaciones de cultivos celulares de plantas, que son comúnmente caracterizados por el requerimiento de mantener condiciones relativamente altas tasa de transferencia de masa y simultáneamente evitar el daño celular debido a las condiciones hidrodinámicas.11application/pdfengUniversidad de Antioquia, Facultad de IngenieríaBioprocesosMedellín, Colombiainfo:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_2df8fbb1https://purl.org/redcol/resource_type/ARTArtículo de investigaciónhttp://purl.org/coar/version/c_970fb48d4fbd8a86Atribución-NoComercial-CompartirIgual 2.5 Colombia (CC BY-NC-SA 2.5 CO)info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/co/http://purl.org/coar/access_right/c_abf2https://creativecommons.org/licenses/by-nc-sa/4.0/Transferencia de masaBioreactoresDinámica de fluidosHidrodinámicaProcesos biológicosEscalado de procesosSimulating gas-liquid mass transfer in a spin filter bioreactorSimulación de la transferencia de masa en un biorreactor de perfusión operado con un filtro rotativoRev. Fac. Ing. Univ. AntioquiaRevista Facultad de Ingeniería Universidad de Antioquia16317475ORIGINALNiñoLilibeth_2015_SimulatingGasLiquid.pdfNiñoLilibeth_2015_SimulatingGasLiquid.pdfArtículo de investigaciónapplication/pdf3377468http://bibliotecadigital.udea.edu.co/bitstream/10495/5988/1/Ni%c3%b1oLilibeth_2015_SimulatingGasLiquid.pdf6baeed17bca371c98cee1c9a973f4af9MD51CC-LICENSElicense_urllicense_urltext/plain; charset=utf-849http://bibliotecadigital.udea.edu.co/bitstream/10495/5988/2/license_url4afdbb8c545fd630ea7db775da747b2fMD52license_textlicense_texttext/html; charset=utf-80http://bibliotecadigital.udea.edu.co/bitstream/10495/5988/3/license_textd41d8cd98f00b204e9800998ecf8427eMD53license_rdflicense_rdfapplication/rdf+xml; charset=utf-80http://bibliotecadigital.udea.edu.co/bitstream/10495/5988/4/license_rdfd41d8cd98f00b204e9800998ecf8427eMD54LICENSElicense.txtlicense.txttext/plain; charset=utf-81748http://bibliotecadigital.udea.edu.co/bitstream/10495/5988/5/license.txt8a4605be74aa9ea9d79846c1fba20a33MD5510495/5988oai:bibliotecadigital.udea.edu.co:10495/59882021-05-07 11:45:23.568Repositorio Institucional Universidad de Antioquiaandres.perez@udea.edu.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 |