The Orphan Response Regulator Aor1 Is a New Relevant Piece in the Complex Puzzle of Streptomyces coelicolor Antibiotic Regulatory Network

ABSTARCT: Streptomyces coelicolor, the best-known biological antibiotic producer, encodes 29 predicted orphan response regulators (RR) with a putative role in the response to environmental stimuli. However, their implication in relation to secondary metabolite production is mostly unexplored. Here,...

Full description

Autores:
Antoraz, Sergio
Rico, Sergio
Rodríguez, Héctor
Sevillano, Laura
Alzate Restrepo, Juan Fernando
Santamaría, Ramón
Díaz, Margarita
Tipo de recurso:
Article of investigation
Fecha de publicación:
2017
Institución:
Universidad de Antioquia
Repositorio:
Repositorio UdeA
Idioma:
eng
OAI Identifier:
oai:bibliotecadigital.udea.edu.co:10495/11573
Acceso en línea:
http://hdl.handle.net/10495/11573
Palabra clave:
Streptomyces coelicolor
Antibiotic production
Crosstalk
Streptomyces
Stress response
Two-component systems
Orphan response regulator
Gen Aor1
Rights
openAccess
License
Atribución 2.5
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network_acronym_str UDEA2
network_name_str Repositorio UdeA
repository_id_str
dc.title.spa.fl_str_mv The Orphan Response Regulator Aor1 Is a New Relevant Piece in the Complex Puzzle of Streptomyces coelicolor Antibiotic Regulatory Network
title The Orphan Response Regulator Aor1 Is a New Relevant Piece in the Complex Puzzle of Streptomyces coelicolor Antibiotic Regulatory Network
spellingShingle The Orphan Response Regulator Aor1 Is a New Relevant Piece in the Complex Puzzle of Streptomyces coelicolor Antibiotic Regulatory Network
Streptomyces coelicolor
Antibiotic production
Crosstalk
Streptomyces
Stress response
Two-component systems
Orphan response regulator
Gen Aor1
title_short The Orphan Response Regulator Aor1 Is a New Relevant Piece in the Complex Puzzle of Streptomyces coelicolor Antibiotic Regulatory Network
title_full The Orphan Response Regulator Aor1 Is a New Relevant Piece in the Complex Puzzle of Streptomyces coelicolor Antibiotic Regulatory Network
title_fullStr The Orphan Response Regulator Aor1 Is a New Relevant Piece in the Complex Puzzle of Streptomyces coelicolor Antibiotic Regulatory Network
title_full_unstemmed The Orphan Response Regulator Aor1 Is a New Relevant Piece in the Complex Puzzle of Streptomyces coelicolor Antibiotic Regulatory Network
title_sort The Orphan Response Regulator Aor1 Is a New Relevant Piece in the Complex Puzzle of Streptomyces coelicolor Antibiotic Regulatory Network
dc.creator.fl_str_mv Antoraz, Sergio
Rico, Sergio
Rodríguez, Héctor
Sevillano, Laura
Alzate Restrepo, Juan Fernando
Santamaría, Ramón
Díaz, Margarita
dc.contributor.author.none.fl_str_mv Antoraz, Sergio
Rico, Sergio
Rodríguez, Héctor
Sevillano, Laura
Alzate Restrepo, Juan Fernando
Santamaría, Ramón
Díaz, Margarita
dc.subject.none.fl_str_mv Streptomyces coelicolor
Antibiotic production
Crosstalk
Streptomyces
Stress response
Two-component systems
Orphan response regulator
Gen Aor1
topic Streptomyces coelicolor
Antibiotic production
Crosstalk
Streptomyces
Stress response
Two-component systems
Orphan response regulator
Gen Aor1
description ABSTARCT: Streptomyces coelicolor, the best-known biological antibiotic producer, encodes 29 predicted orphan response regulators (RR) with a putative role in the response to environmental stimuli. However, their implication in relation to secondary metabolite production is mostly unexplored. Here, we show how the deletion of the orphan RR Aor1 (SCO2281) provoked a drastic decrease in the production of the three main antibiotics produced by S. coelicolor and a delay in morphological differentiation. With the aim to better understand the transcriptional events underpinning these phenotypes, and the global role of Aor1 in Streptomyces, a transcriptional fingerprint of the Δaor1 mutant was compared to a wild-type strain. RNA-Seq analysis revealed that the deletion of this orphan regulator affects a strikingly high number of genes, such as the genes involved in secondary metabolism, which matches the antibiotic production profiles observed. Of particular note, the sigma factor SigB and all of the genes comprising its regulon were up regulated in the mutant. Our results show that this event links osmotic stress to secondary metabolite production in S. coelicolor and indicates that the RR encoded by aor1 could be a key regulator in both of these processes. Key words: Streptomyces, two-component systems, antibiotic production, orphan response regulator, crosstalk, stress response
publishDate 2017
dc.date.issued.none.fl_str_mv 2017
dc.date.accessioned.none.fl_str_mv 2019-07-26T22:12:14Z
dc.date.available.none.fl_str_mv 2019-07-26T22:12:14Z
dc.type.spa.fl_str_mv info:eu-repo/semantics/article
dc.type.coarversion.fl_str_mv http://purl.org/coar/version/c_970fb48d4fbd8a85
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dc.type.local.spa.fl_str_mv Artículo de investigación
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dc.identifier.citation.spa.fl_str_mv Antoraz S, Rico S, Rodríguez H, Sevillano L, Alzate JF, Santamaría RI, Díaz M. The Orphan Response Regulator Aor1 Is a New Relevant Piece in the Complex Puzzle of Streptomyces coelicolor Antibiotic Regulatory Network. Front Microbiol. 2017 Dec 12;8:2444
dc.identifier.issn.none.fl_str_mv 1664-302X
dc.identifier.uri.none.fl_str_mv http://hdl.handle.net/10495/11573
dc.identifier.doi.none.fl_str_mv 10.3389/fmicb.2017.02444
identifier_str_mv Antoraz S, Rico S, Rodríguez H, Sevillano L, Alzate JF, Santamaría RI, Díaz M. The Orphan Response Regulator Aor1 Is a New Relevant Piece in the Complex Puzzle of Streptomyces coelicolor Antibiotic Regulatory Network. Front Microbiol. 2017 Dec 12;8:2444
1664-302X
10.3389/fmicb.2017.02444
url http://hdl.handle.net/10495/11573
dc.language.iso.spa.fl_str_mv eng
language eng
dc.relation.ispartofjournalabbrev.spa.fl_str_mv Front Microbiol
dc.rights.*.fl_str_mv Atribución 2.5
dc.rights.spa.fl_str_mv info:eu-repo/semantics/openAccess
dc.rights.uri.*.fl_str_mv http://creativecommons.org/licenses/by/2.5/co/
dc.rights.accessrights.spa.fl_str_mv http://purl.org/coar/access_right/c_abf2
dc.rights.creativecommons.spa.fl_str_mv https://creativecommons.org/licenses/by/4.0/
rights_invalid_str_mv Atribución 2.5
http://creativecommons.org/licenses/by/2.5/co/
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eu_rights_str_mv openAccess
dc.format.extent.spa.fl_str_mv 11
dc.format.mimetype.spa.fl_str_mv application/pdf
dc.publisher.spa.fl_str_mv Frontiers Research Foundation
dc.publisher.group.spa.fl_str_mv Grupo de Parasitología, Universidad de Antioquia
dc.publisher.place.spa.fl_str_mv Suiza
institution Universidad de Antioquia
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spelling Antoraz, SergioRico, SergioRodríguez, HéctorSevillano, LauraAlzate Restrepo, Juan FernandoSantamaría, RamónDíaz, Margarita2019-07-26T22:12:14Z2019-07-26T22:12:14Z2017Antoraz S, Rico S, Rodríguez H, Sevillano L, Alzate JF, Santamaría RI, Díaz M. The Orphan Response Regulator Aor1 Is a New Relevant Piece in the Complex Puzzle of Streptomyces coelicolor Antibiotic Regulatory Network. Front Microbiol. 2017 Dec 12;8:24441664-302Xhttp://hdl.handle.net/10495/1157310.3389/fmicb.2017.02444ABSTARCT: Streptomyces coelicolor, the best-known biological antibiotic producer, encodes 29 predicted orphan response regulators (RR) with a putative role in the response to environmental stimuli. However, their implication in relation to secondary metabolite production is mostly unexplored. Here, we show how the deletion of the orphan RR Aor1 (SCO2281) provoked a drastic decrease in the production of the three main antibiotics produced by S. coelicolor and a delay in morphological differentiation. With the aim to better understand the transcriptional events underpinning these phenotypes, and the global role of Aor1 in Streptomyces, a transcriptional fingerprint of the Δaor1 mutant was compared to a wild-type strain. RNA-Seq analysis revealed that the deletion of this orphan regulator affects a strikingly high number of genes, such as the genes involved in secondary metabolism, which matches the antibiotic production profiles observed. Of particular note, the sigma factor SigB and all of the genes comprising its regulon were up regulated in the mutant. Our results show that this event links osmotic stress to secondary metabolite production in S. coelicolor and indicates that the RR encoded by aor1 could be a key regulator in both of these processes. 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