Electrophoretic karyotype of environmental isolates of Paracoccidioides brasiliensis
ABSTRACT: Five of the 12 environmental isolates of the dimorphic fungus Paracoccidioides brasiliensis known to date, were analysed by contour-clamped homogeneous electric field gel electrophoresis (CHEF). The electrophoretic pattern was shown to consist of five bands, with molecular size ranging fro...
- Autores:
-
Montoya Restrepo, Astrid Elena
Álvarez Aldana, Adalucy
Restrepo Moreno, Ángela
Mcewen Ochoa, Juan Guillermo
- Tipo de recurso:
- Article of journal
- Fecha de publicación:
- 1999
- Institución:
- Universidad de Antioquia
- Repositorio:
- Repositorio UdeA
- Idioma:
- eng
- OAI Identifier:
- oai:bibliotecadigital.udea.edu.co:10495/24121
- Acceso en línea:
- http://hdl.handle.net/10495/24121
- Palabra clave:
- Paracoccidioides
Cariotipo
Karyotype
- Rights
- openAccess
- License
- http://creativecommons.org/licenses/by-nc/2.5/co/
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Electrophoretic karyotype of environmental isolates of Paracoccidioides brasiliensis |
| title |
Electrophoretic karyotype of environmental isolates of Paracoccidioides brasiliensis |
| spellingShingle |
Electrophoretic karyotype of environmental isolates of Paracoccidioides brasiliensis Paracoccidioides Cariotipo Karyotype |
| title_short |
Electrophoretic karyotype of environmental isolates of Paracoccidioides brasiliensis |
| title_full |
Electrophoretic karyotype of environmental isolates of Paracoccidioides brasiliensis |
| title_fullStr |
Electrophoretic karyotype of environmental isolates of Paracoccidioides brasiliensis |
| title_full_unstemmed |
Electrophoretic karyotype of environmental isolates of Paracoccidioides brasiliensis |
| title_sort |
Electrophoretic karyotype of environmental isolates of Paracoccidioides brasiliensis |
| dc.creator.fl_str_mv |
Montoya Restrepo, Astrid Elena Álvarez Aldana, Adalucy Restrepo Moreno, Ángela Mcewen Ochoa, Juan Guillermo |
| dc.contributor.author.none.fl_str_mv |
Montoya Restrepo, Astrid Elena Álvarez Aldana, Adalucy Restrepo Moreno, Ángela Mcewen Ochoa, Juan Guillermo |
| dc.subject.decs.none.fl_str_mv |
Paracoccidioides Cariotipo Karyotype |
| topic |
Paracoccidioides Cariotipo Karyotype |
| description |
ABSTRACT: Five of the 12 environmental isolates of the dimorphic fungus Paracoccidioides brasiliensis known to date, were analysed by contour-clamped homogeneous electric field gel electrophoresis (CHEF). The electrophoretic pattern was shown to consist of five bands, with molecular size ranging from 3·2 to 10 Mb, a model quite similar to the one found in the clinical isolates previously tested and used here as controls. However, one of the bands in the environmental isolates had a lesser weight (7·2 Mb), than the one corresponding to the clinical counterparts (8·8 Mb). This resulted in a smaller genome, approximately 29·7 Mb. The small differences that were found indicate the presence of chromosome polymorphism in this fungus. |
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1999 |
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1999 |
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2021-11-15T19:44:55Z |
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2021-11-15T19:44:55Z |
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info:eu-repo/semantics/article |
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Artículo de revista |
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1369-3786 |
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http://hdl.handle.net/10495/24121 |
| dc.identifier.doi.none.fl_str_mv |
10.1080/j.1365-280X.1999.00217.x |
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1460-2709 |
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1369-3786 10.1080/j.1365-280X.1999.00217.x 1460-2709 |
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http://hdl.handle.net/10495/24121 |
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eng |
| language |
eng |
| dc.relation.ispartofjournalabbrev.spa.fl_str_mv |
Med. Mycol. |
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info:eu-repo/semantics/openAccess |
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4 |
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Oxford University Press |
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Micología Médica y Experimental |
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Oxford, Inglaterra |
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Montoya Restrepo, Astrid ElenaÁlvarez Aldana, AdalucyRestrepo Moreno, ÁngelaMcewen Ochoa, Juan Guillermo2021-11-15T19:44:55Z2021-11-15T19:44:55Z19991369-3786http://hdl.handle.net/10495/2412110.1080/j.1365-280X.1999.00217.x1460-2709ABSTRACT: Five of the 12 environmental isolates of the dimorphic fungus Paracoccidioides brasiliensis known to date, were analysed by contour-clamped homogeneous electric field gel electrophoresis (CHEF). The electrophoretic pattern was shown to consist of five bands, with molecular size ranging from 3·2 to 10 Mb, a model quite similar to the one found in the clinical isolates previously tested and used here as controls. However, one of the bands in the environmental isolates had a lesser weight (7·2 Mb), than the one corresponding to the clinical counterparts (8·8 Mb). This resulted in a smaller genome, approximately 29·7 Mb. The small differences that were found indicate the presence of chromosome polymorphism in this fungus.COL00137094application/pdfengOxford University PressMicología Médica y ExperimentalOxford, Inglaterrainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1http://purl.org/redcol/resource_type/CJournalArticleArtículo de revistahttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc/2.5/co/http://purl.org/coar/access_right/c_abf2https://creativecommons.org/licenses/by-nc/4.0/Electrophoretic karyotype of environmental isolates of Paracoccidioides brasiliensisParacoccidioidesCariotipoKaryotypeMed. Mycol.Medical Mycology219222373ORIGINALRestrepoAngela_1999_ElectrophoreticIsolates.pdfRestrepoAngela_1999_ElectrophoreticIsolates.pdfArtículo de revistaapplication/pdf103565http://bibliotecadigital.udea.edu.co/bitstream/10495/24121/1/RestrepoAngela_1999_ElectrophoreticIsolates.pdfe6a56e25aea9f99677a6000362b9b299MD51CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8933http://bibliotecadigital.udea.edu.co/bitstream/10495/24121/2/license_rdfc0c92b0ffc8b7d22d9cf56754a416a76MD52LICENSElicense.txtlicense.txttext/plain; charset=utf-81748http://bibliotecadigital.udea.edu.co/bitstream/10495/24121/3/license.txt8a4605be74aa9ea9d79846c1fba20a33MD5310495/24121oai:bibliotecadigital.udea.edu.co:10495/241212022-04-22 10:21:41.198Repositorio Institucional Universidad de Antioquiaandres.perez@udea.edu.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 |