Electrophoretic karyotype of environmental isolates of Paracoccidioides brasiliensis
ABSTRACT: Five of the 12 environmental isolates of the dimorphic fungus Paracoccidioides brasiliensis known to date, were analysed by contour-clamped homogeneous electric field gel electrophoresis (CHEF). The electrophoretic pattern was shown to consist of five bands, with molecular size ranging fro...
- Autores:
-
Montoya Restrepo, Astrid Elena
Álvarez Aldana, Adalucy
Restrepo Moreno, Ángela
Mcewen Ochoa, Juan Guillermo
- Tipo de recurso:
- Article of journal
- Fecha de publicación:
- 1999
- Institución:
- Universidad de Antioquia
- Repositorio:
- Repositorio UdeA
- Idioma:
- eng
- OAI Identifier:
- oai:bibliotecadigital.udea.edu.co:10495/24121
- Acceso en línea:
- http://hdl.handle.net/10495/24121
- Palabra clave:
- Paracoccidioides
Cariotipo
Karyotype
- Rights
- openAccess
- License
- http://creativecommons.org/licenses/by-nc/2.5/co/
| Summary: | ABSTRACT: Five of the 12 environmental isolates of the dimorphic fungus Paracoccidioides brasiliensis known to date, were analysed by contour-clamped homogeneous electric field gel electrophoresis (CHEF). The electrophoretic pattern was shown to consist of five bands, with molecular size ranging from 3·2 to 10 Mb, a model quite similar to the one found in the clinical isolates previously tested and used here as controls. However, one of the bands in the environmental isolates had a lesser weight (7·2 Mb), than the one corresponding to the clinical counterparts (8·8 Mb). This resulted in a smaller genome, approximately 29·7 Mb. The small differences that were found indicate the presence of chromosome polymorphism in this fungus. |
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