Molecular identification and genotyping of Streptococcus mutans from saliva samples of children in Medellin, Colombia
ABSTRACT: Introduction and objective: Tooth decay is one of the most prevalent infectious diseases of the oral cavity. The aim of this study was to determine the prevalence of Streptoccocus mutans and its genotypes in saliva samples from 6 and 7 year old children with and without dental caries. Mate...
- Autores:
-
Arévalo Ruano, Mary
Canacuán Melo, Flor Yuranny
Echeverry Chica, Julián
Salazar González, Clara Lina
Martínez Delgado, Cecilia María
Martínez Pabón, María Cecilia
Correa Ochoa, Margarita María
Cienfuegos Gallet, Astrid Vanessa
- Tipo de recurso:
- Article of investigation
- Fecha de publicación:
- 2014
- Institución:
- Universidad de Antioquia
- Repositorio:
- Repositorio UdeA
- Idioma:
- eng
- OAI Identifier:
- oai:bibliotecadigital.udea.edu.co:10495/21490
- Acceso en línea:
- http://hdl.handle.net/10495/21490
https://revistas.ces.edu.co/index.php/odontologia/article/view/3241
- Palabra clave:
- Streptococcus mutans
Reacción en Cadena de la Polimerasa
Polymerase Chain Reaction
Caries dental en niños
Características genotípicas
- Rights
- openAccess
- License
- http://creativecommons.org/licenses/by-nc-nd/2.5/co/
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|
| dc.title.spa.fl_str_mv |
Molecular identification and genotyping of Streptococcus mutans from saliva samples of children in Medellin, Colombia |
| dc.title.alternative.spa.fl_str_mv |
Identificación molecular y genotipificación de Streptococcus mutans de muestras de saliva de niños de Medellín, Colombia |
| title |
Molecular identification and genotyping of Streptococcus mutans from saliva samples of children in Medellin, Colombia |
| spellingShingle |
Molecular identification and genotyping of Streptococcus mutans from saliva samples of children in Medellin, Colombia Streptococcus mutans Reacción en Cadena de la Polimerasa Polymerase Chain Reaction Caries dental en niños Características genotípicas |
| title_short |
Molecular identification and genotyping of Streptococcus mutans from saliva samples of children in Medellin, Colombia |
| title_full |
Molecular identification and genotyping of Streptococcus mutans from saliva samples of children in Medellin, Colombia |
| title_fullStr |
Molecular identification and genotyping of Streptococcus mutans from saliva samples of children in Medellin, Colombia |
| title_full_unstemmed |
Molecular identification and genotyping of Streptococcus mutans from saliva samples of children in Medellin, Colombia |
| title_sort |
Molecular identification and genotyping of Streptococcus mutans from saliva samples of children in Medellin, Colombia |
| dc.creator.fl_str_mv |
Arévalo Ruano, Mary Canacuán Melo, Flor Yuranny Echeverry Chica, Julián Salazar González, Clara Lina Martínez Delgado, Cecilia María Martínez Pabón, María Cecilia Correa Ochoa, Margarita María Cienfuegos Gallet, Astrid Vanessa |
| dc.contributor.author.none.fl_str_mv |
Arévalo Ruano, Mary Canacuán Melo, Flor Yuranny Echeverry Chica, Julián Salazar González, Clara Lina Martínez Delgado, Cecilia María Martínez Pabón, María Cecilia Correa Ochoa, Margarita María Cienfuegos Gallet, Astrid Vanessa |
| dc.subject.decs.none.fl_str_mv |
Streptococcus mutans Reacción en Cadena de la Polimerasa Polymerase Chain Reaction |
| topic |
Streptococcus mutans Reacción en Cadena de la Polimerasa Polymerase Chain Reaction Caries dental en niños Características genotípicas |
| dc.subject.proposal.spa.fl_str_mv |
Caries dental en niños Características genotípicas |
| description |
ABSTRACT: Introduction and objective: Tooth decay is one of the most prevalent infectious diseases of the oral cavity. The aim of this study was to determine the prevalence of Streptoccocus mutans and its genotypes in saliva samples from 6 and 7 year old children with and without dental caries. Materials and methods: Forty seven saliva samples were obtained from 6-7 year-old children, randomly selected from a Public School in Medellin, Colombia. Oral examinations in order to determine the DMF-T Index (Decay, Missing and Filling Teeth) were carried out. Isolates were identified by the Streptoccocus sobrinus and Streptococcus mutans PCR, API 20STREP and 16S rRNA sequence analysis. Genotypes c, e, f and k were detected by PCR. Results: Prevalence of S. mutans was 14.9%. Of the 47 saliva samples, 57.4% (27) corresponded to children with dental caries, and 8.5% (4) were positive for S. mutans genotype c, 2.1% (1 each) genotype f, genotype k, and genotype c and k, respectively. Conclusion: Prevalence of S. mutans was lower than in previous studies of Colombian children with similar demographic characteristics. Streptoccocus mutans genotype c, f and k were found in children with caries but not in the group without caries. This is the first report of S. mutans genotype K in Colombia; this genotype requires further study to clarify its relation with dental caries and cardiovascular disease in Colombia. |
| publishDate |
2014 |
| dc.date.issued.none.fl_str_mv |
2014 |
| dc.date.accessioned.none.fl_str_mv |
2021-08-03T20:20:03Z |
| dc.date.available.none.fl_str_mv |
2021-08-03T20:20:03Z |
| dc.type.spa.fl_str_mv |
info:eu-repo/semantics/article |
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http://purl.org/coar/version/c_970fb48d4fbd8a85 |
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info:eu-repo/semantics/publishedVersion |
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http://purl.org/coar/resource_type/c_2df8fbb1 |
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https://purl.org/redcol/resource_type/ART |
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Artículo de investigación |
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http://purl.org/coar/resource_type/c_2df8fbb1 |
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publishedVersion |
| dc.identifier.issn.none.fl_str_mv |
0120-971X |
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http://hdl.handle.net/10495/21490 |
| dc.identifier.eissn.none.fl_str_mv |
2215-9185 |
| dc.identifier.url.spa.fl_str_mv |
https://revistas.ces.edu.co/index.php/odontologia/article/view/3241 |
| identifier_str_mv |
0120-971X 2215-9185 |
| url |
http://hdl.handle.net/10495/21490 https://revistas.ces.edu.co/index.php/odontologia/article/view/3241 |
| dc.language.iso.spa.fl_str_mv |
eng |
| language |
eng |
| dc.relation.ispartofjournalabbrev.spa.fl_str_mv |
CES odontol. |
| dc.rights.spa.fl_str_mv |
info:eu-repo/semantics/openAccess |
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http://creativecommons.org/licenses/by-nc-nd/2.5/co/ |
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https://creativecommons.org/licenses/by-nc-nd/4.0/ |
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openAccess |
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http://creativecommons.org/licenses/by-nc-nd/2.5/co/ http://purl.org/coar/access_right/c_abf2 https://creativecommons.org/licenses/by-nc-nd/4.0/ |
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14 |
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application/pdf |
| dc.publisher.spa.fl_str_mv |
Universidad CES, Facultad de Odontología |
| dc.publisher.group.spa.fl_str_mv |
Grupo de Investigación en Patología Oral Periodoncia y Cirugía Alvéolo-Dentaria (POPCAD) Microbiología Molecular |
| dc.publisher.place.spa.fl_str_mv |
Medellín, Colombia |
| institution |
Universidad de Antioquia |
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http://bibliotecadigital.udea.edu.co/bitstream/10495/21490/2/license_rdf http://bibliotecadigital.udea.edu.co/bitstream/10495/21490/3/license.txt http://bibliotecadigital.udea.edu.co/bitstream/10495/21490/1/Ar%c3%a9valoMary_2014_StreptococcusSalivaChildren.pdf |
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andres.perez@udea.edu.co |
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Arévalo Ruano, MaryCanacuán Melo, Flor YurannyEcheverry Chica, JuliánSalazar González, Clara LinaMartínez Delgado, Cecilia MaríaMartínez Pabón, María CeciliaCorrea Ochoa, Margarita MaríaCienfuegos Gallet, Astrid Vanessa2021-08-03T20:20:03Z2021-08-03T20:20:03Z20140120-971Xhttp://hdl.handle.net/10495/214902215-9185https://revistas.ces.edu.co/index.php/odontologia/article/view/3241ABSTRACT: Introduction and objective: Tooth decay is one of the most prevalent infectious diseases of the oral cavity. The aim of this study was to determine the prevalence of Streptoccocus mutans and its genotypes in saliva samples from 6 and 7 year old children with and without dental caries. Materials and methods: Forty seven saliva samples were obtained from 6-7 year-old children, randomly selected from a Public School in Medellin, Colombia. Oral examinations in order to determine the DMF-T Index (Decay, Missing and Filling Teeth) were carried out. Isolates were identified by the Streptoccocus sobrinus and Streptococcus mutans PCR, API 20STREP and 16S rRNA sequence analysis. Genotypes c, e, f and k were detected by PCR. Results: Prevalence of S. mutans was 14.9%. Of the 47 saliva samples, 57.4% (27) corresponded to children with dental caries, and 8.5% (4) were positive for S. mutans genotype c, 2.1% (1 each) genotype f, genotype k, and genotype c and k, respectively. Conclusion: Prevalence of S. mutans was lower than in previous studies of Colombian children with similar demographic characteristics. Streptoccocus mutans genotype c, f and k were found in children with caries but not in the group without caries. This is the first report of S. mutans genotype K in Colombia; this genotype requires further study to clarify its relation with dental caries and cardiovascular disease in Colombia.RESUMEN: Introducción y objetivo: La caries dental es una de las enfermedades infecciosas de la cavidad oral más comunes en el mundo. El objetivo de este estudio fue determinar la prevalencia de Streptococcus mutans y sus genotipos en muestras de saliva de niños de 6 y 7 años, con y sin caries dental. Materiales y métodos: Cuarenta y siete (47) muestras de saliva fueron obtenidas de niños de 6-7 años, eleccionados aleatoriamente de una escuela pública en Medellín, Colombia. Se realizaron evaluaciones orales para determinar el índice COP-D (número de dientes permanentes cariados, obturados y perdidos). Los aislamientos fueron identificados mediante PCR para diferenciación de S. sobrinus y S. mutans , API-20STREP y análisis de ARNr 16S. Se determinaron mediante PCR los genotipos c, e, f y k en las cepas identificadas como S. mutans. Resultados: La prevalencia de S. mutans fue 14,9%. De las 47 muestras de saliva, 57,4% (27) correspondieron a niños con caries dental, y 8,5 % (4), fueron positivas para S. mutans genotipo c , 2,1 % (1 cada una) genotipo f, genotipo k y genotipo c y k, respectivamente. Conclusión: La prevalencia de S. mutans fue menor que la reportada en estudios previos de niños colombianos con características demográficas similares. Se encontró presencia de genotipos c, f y k de S. mutans en niños con caries dental, pero no en el grupo de niños sin caries dental. Este es el primer estudio en reportar la presencia del genotipo k en Colombia. Es necesario realizar más investigaciones en nuestro país para aclarar la relación de S. mutans genotipo k con caries dental y enfermedad cardiovascular.COL0013746COL001007414application/pdfengUniversidad CES, Facultad de OdontologíaGrupo de Investigación en Patología Oral Periodoncia y Cirugía Alvéolo-Dentaria (POPCAD)Microbiología MolecularMedellín, Colombiainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_2df8fbb1https://purl.org/redcol/resource_type/ARTArtículo de investigaciónhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-nd/2.5/co/http://purl.org/coar/access_right/c_abf2https://creativecommons.org/licenses/by-nc-nd/4.0/Molecular identification and genotyping of Streptococcus mutans from saliva samples of children in Medellin, ColombiaIdentificación molecular y genotipificación de Streptococcus mutans de muestras de saliva de niños de Medellín, ColombiaStreptococcus mutansReacción en Cadena de la PolimerasaPolymerase Chain ReactionCaries dental en niñosCaracterísticas genotípicasCES odontol.CES Odontología4760272CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8823http://bibliotecadigital.udea.edu.co/bitstream/10495/21490/2/license_rdfb88b088d9957e670ce3b3fbe2eedbc13MD52LICENSElicense.txtlicense.txttext/plain; charset=utf-81748http://bibliotecadigital.udea.edu.co/bitstream/10495/21490/3/license.txt8a4605be74aa9ea9d79846c1fba20a33MD53ORIGINALArévaloMary_2014_StreptococcusSalivaChildren.pdfArévaloMary_2014_StreptococcusSalivaChildren.pdfArtículo de investigaciónapplication/pdf216111http://bibliotecadigital.udea.edu.co/bitstream/10495/21490/1/Ar%c3%a9valoMary_2014_StreptococcusSalivaChildren.pdf4076a9541c7ab4e521c71212f394f286MD5110495/21490oai:bibliotecadigital.udea.edu.co:10495/214902022-04-22 10:19:18.745Repositorio Institucional Universidad de Antioquiaandres.perez@udea.edu.coTk9URTogUExBQ0UgWU9VUiBPV04gTElDRU5TRSBIRVJFClRoaXMgc2FtcGxlIGxpY2Vuc2UgaXMgcHJvdmlkZWQgZm9yIGluZm9ybWF0aW9uYWwgcHVycG9zZXMgb25seS4KCk5PTi1FWENMVVNJVkUgRElTVFJJQlVUSU9OIExJQ0VOU0UKCkJ5IHNpZ25pbmcgYW5kIHN1Ym1pdHRpbmcgdGhpcyBsaWNlbnNlLCB5b3UgKHRoZSBhdXRob3Iocykgb3IgY29weXJpZ2h0Cm93bmVyKSBncmFudHMgdG8gRFNwYWNlIFVuaXZlcnNpdHkgKERTVSkgdGhlIG5vbi1leGNsdXNpdmUgcmlnaHQgdG8gcmVwcm9kdWNlLAp0cmFuc2xhdGUgKGFzIGRlZmluZWQgYmVsb3cpLCBhbmQvb3IgZGlzdHJpYnV0ZSB5b3VyIHN1Ym1pc3Npb24gKGluY2x1ZGluZwp0aGUgYWJzdHJhY3QpIHdvcmxkd2lkZSBpbiBwcmludCBhbmQgZWxlY3Ryb25pYyBmb3JtYXQgYW5kIGluIGFueSBtZWRpdW0sCmluY2x1ZGluZyBidXQgbm90IGxpbWl0ZWQgdG8gYXVkaW8gb3IgdmlkZW8uCgpZb3UgYWdyZWUgdGhhdCBEU1UgbWF5LCB3aXRob3V0IGNoYW5naW5nIHRoZSBjb250ZW50LCB0cmFuc2xhdGUgdGhlCnN1Ym1pc3Npb24gdG8gYW55IG1lZGl1bSBvciBmb3JtYXQgZm9yIHRoZSBwdXJwb3NlIG9mIHByZXNlcnZhdGlvbi4KCllvdSBhbHNvIGFncmVlIHRoYXQgRFNVIG1heSBrZWVwIG1vcmUgdGhhbiBvbmUgY29weSBvZiB0aGlzIHN1Ym1pc3Npb24gZm9yCnB1cnBvc2VzIG9mIHNlY3VyaXR5LCBiYWNrLXVwIGFuZCBwcmVzZXJ2YXRpb24uCgpZb3UgcmVwcmVzZW50IHRoYXQgdGhlIHN1Ym1pc3Npb24gaXMgeW91ciBvcmlnaW5hbCB3b3JrLCBhbmQgdGhhdCB5b3UgaGF2ZQp0aGUgcmlnaHQgdG8gZ3JhbnQgdGhlIHJpZ2h0cyBjb250YWluZWQgaW4gdGhpcyBsaWNlbnNlLiBZb3UgYWxzbyByZXByZXNlbnQKdGhhdCB5b3VyIHN1Ym1pc3Npb24gZG9lcyBub3QsIHRvIHRoZSBiZXN0IG9mIHlvdXIga25vd2xlZGdlLCBpbmZyaW5nZSB1cG9uCmFueW9uZSdzIGNvcHlyaWdodC4KCklmIHRoZSBzdWJtaXNzaW9uIGNvbnRhaW5zIG1hdGVyaWFsIGZvciB3aGljaCB5b3UgZG8gbm90IGhvbGQgY29weXJpZ2h0LAp5b3UgcmVwcmVzZW50IHRoYXQgeW91IGhhdmUgb2J0YWluZWQgdGhlIHVucmVzdHJpY3RlZCBwZXJtaXNzaW9uIG9mIHRoZQpjb3B5cmlnaHQgb3duZXIgdG8gZ3JhbnQgRFNVIHRoZSByaWdodHMgcmVxdWlyZWQgYnkgdGhpcyBsaWNlbnNlLCBhbmQgdGhhdApzdWNoIHRoaXJkLXBhcnR5IG93bmVkIG1hdGVyaWFsIGlzIGNsZWFybHkgaWRlbnRpZmllZCBhbmQgYWNrbm93bGVkZ2VkCndpdGhpbiB0aGUgdGV4dCBvciBjb250ZW50IG9mIHRoZSBzdWJtaXNzaW9uLgoKSUYgVEhFIFNVQk1JU1NJT04gSVMgQkFTRUQgVVBPTiBXT1JLIFRIQVQgSEFTIEJFRU4gU1BPTlNPUkVEIE9SIFNVUFBPUlRFRApCWSBBTiBBR0VOQ1kgT1IgT1JHQU5JWkFUSU9OIE9USEVSIFRIQU4gRFNVLCBZT1UgUkVQUkVTRU5UIFRIQVQgWU9VIEhBVkUKRlVMRklMTEVEIEFOWSBSSUdIVCBPRiBSRVZJRVcgT1IgT1RIRVIgT0JMSUdBVElPTlMgUkVRVUlSRUQgQlkgU1VDSApDT05UUkFDVCBPUiBBR1JFRU1FTlQuCgpEU1Ugd2lsbCBjbGVhcmx5IGlkZW50aWZ5IHlvdXIgbmFtZShzKSBhcyB0aGUgYXV0aG9yKHMpIG9yIG93bmVyKHMpIG9mIHRoZQpzdWJtaXNzaW9uLCBhbmQgd2lsbCBub3QgbWFrZSBhbnkgYWx0ZXJhdGlvbiwgb3RoZXIgdGhhbiBhcyBhbGxvd2VkIGJ5IHRoaXMKbGljZW5zZSwgdG8geW91ciBzdWJtaXNzaW9uLgo= |