Bone morphogenetic protein 2 : heterologous expression and potential in bone regeneration

ABSTRACT: Currently, bone morphogenetic protein 2 (BMP‐2) is one of the two osteoinductive growth factors used in medical devices to promote bone formation. Typically, this protein is bought from commercial houses at high rates and in small quantities that are not enough to cover clinical needs. Because of this, it has been proposed that research centers use their own heterologous expression systems to have a constant supply of BMP‐2. The aim of this study was to standardize the heterologous expression of BMP‐2 and evaluate its osteoinductive activity in vitro. Our procedure for expression and purification was based on recombinant DNA technology using the plasmid pET‐28 and IPTG as inductor. After extracting the protein from inclusion bodies, folding it and modifying it via a redox system, we observed via electrophoresis a 26 kDa dimer. We evaluated its osteoinductive activity in myoblastic C2C12 by quantifying enzymatically the activity of alkaline phosphate (ALP) and staining mineralization nodules. ALP activity is proportional to BMP‐2 concentration, increasing 90% at 3 μg/mL. These cells form calcium nodules, mineralizing 50% of the area.

• Autores: Mesa Restrepo, Andrea; Alzate, Juan Fernando; Patiño González, Edwin Bairon
• Tipo de recurso: Article of investigation
• Fecha de publicación: 2021
• Institución: Universidad de Antioquia
• Repositorio: Repositorio UdeA
• Idioma: eng
• OAI Identifier: oai:bibliotecadigital.udea.edu.co:10495/19441
• Acceso en línea: http://hdl.handle.net/10495/19441
• Palabra clave: Bone Regeneration; Fosfatasa alcalina; Alkaline Phosphatase; Células C2C12; Cells C2C12; Renaturalización; Refolding protein; rhBMP‐2; http://id.nlm.nih.gov/mesh/D001861
• Rights: openAccess
• License: Atribución-NoComercial-CompartirIgual 2.5 Colombia