Polyphenols distribution and reserve substances analysis in cocoa somatic embryogenesis

ABSTRACT: In order to understand the causes of lack of regeneration in cacao somatic embryos, two cacao varieties with different responses to regeneration potential were described based on their capacity to store different compounds. It is well known that seed reserves play a central role in the reg...

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Autores:
Gallego Rúa, Adriana María
Henao Ramírez, Ana María
Urrea Trujillo, Aura Inés
Atehortúa Garcés, Lucía
Tipo de recurso:
Article of investigation
Fecha de publicación:
2016
Institución:
Universidad de Antioquia
Repositorio:
Repositorio UdeA
Idioma:
eng
OAI Identifier:
oai:bibliotecadigital.udea.edu.co:10495/20379
Acceso en línea:
http://hdl.handle.net/10495/20379
Palabra clave:
Antioxidants
Antioxidantes
Histology
Histología
Plant Somatic Embryogenesis Techniques
Técnicas de Embriogénesis Somática de Plantas
Banco de semillas del suelo
Soil seed banks
Embriogénesis somática
Somatic embryogenesis
Recalcitrancia
Recalcitrance
http://aims.fao.org/aos/agrovoc/c_35298
http://aims.fao.org/aos/agrovoc/c_36911
Rights
openAccess
License
http://creativecommons.org/licenses/by-nc-sa/2.5/co/
id UDEA2_a2a3174a4f6da318a5d72e4c351d72d3
oai_identifier_str oai:bibliotecadigital.udea.edu.co:10495/20379
network_acronym_str UDEA2
network_name_str Repositorio UdeA
repository_id_str
dc.title.spa.fl_str_mv Polyphenols distribution and reserve substances analysis in cocoa somatic embryogenesis
dc.title.alternative.spa.fl_str_mv Análisis de distribución de polifenoles y sustancias de reserva en embriogénesis somática de cacao
title Polyphenols distribution and reserve substances analysis in cocoa somatic embryogenesis
spellingShingle Polyphenols distribution and reserve substances analysis in cocoa somatic embryogenesis
Antioxidants
Antioxidantes
Histology
Histología
Plant Somatic Embryogenesis Techniques
Técnicas de Embriogénesis Somática de Plantas
Banco de semillas del suelo
Soil seed banks
Embriogénesis somática
Somatic embryogenesis
Recalcitrancia
Recalcitrance
http://aims.fao.org/aos/agrovoc/c_35298
http://aims.fao.org/aos/agrovoc/c_36911
title_short Polyphenols distribution and reserve substances analysis in cocoa somatic embryogenesis
title_full Polyphenols distribution and reserve substances analysis in cocoa somatic embryogenesis
title_fullStr Polyphenols distribution and reserve substances analysis in cocoa somatic embryogenesis
title_full_unstemmed Polyphenols distribution and reserve substances analysis in cocoa somatic embryogenesis
title_sort Polyphenols distribution and reserve substances analysis in cocoa somatic embryogenesis
dc.creator.fl_str_mv Gallego Rúa, Adriana María
Henao Ramírez, Ana María
Urrea Trujillo, Aura Inés
Atehortúa Garcés, Lucía
dc.contributor.author.none.fl_str_mv Gallego Rúa, Adriana María
Henao Ramírez, Ana María
Urrea Trujillo, Aura Inés
Atehortúa Garcés, Lucía
dc.subject.decs.none.fl_str_mv Antioxidants
Antioxidantes
Histology
Histología
Plant Somatic Embryogenesis Techniques
Técnicas de Embriogénesis Somática de Plantas
topic Antioxidants
Antioxidantes
Histology
Histología
Plant Somatic Embryogenesis Techniques
Técnicas de Embriogénesis Somática de Plantas
Banco de semillas del suelo
Soil seed banks
Embriogénesis somática
Somatic embryogenesis
Recalcitrancia
Recalcitrance
http://aims.fao.org/aos/agrovoc/c_35298
http://aims.fao.org/aos/agrovoc/c_36911
dc.subject.agrovoc.none.fl_str_mv Banco de semillas del suelo
Soil seed banks
Embriogénesis somática
Somatic embryogenesis
dc.subject.proposal.spa.fl_str_mv Recalcitrancia
Recalcitrance
dc.subject.agrovocuri.none.fl_str_mv http://aims.fao.org/aos/agrovoc/c_35298
http://aims.fao.org/aos/agrovoc/c_36911
description ABSTRACT: In order to understand the causes of lack of regeneration in cacao somatic embryos, two cacao varieties with different responses to regeneration potential were described based on their capacity to store different compounds. It is well known that seed reserves play a central role in the regenerative capability of somatic embryos; thus, we followed histochemical changes and reserve fluctuations of proteins, polysaccharides and polyphenols during somatic embryogenesis (SE) in the two cacao varieties. The study showed that, in somatic embryos of the regenerating variety, polyphenols were localized mainly in the periphery of the embryo (epidermal cells) and proteins were the main storage substance in the embryo expression medium, while the non-regenerating variety had a high presence of polysaccharides with random distribution of polyphenols at the end of the embryo induction step.
publishDate 2016
dc.date.issued.none.fl_str_mv 2016
dc.date.accessioned.none.fl_str_mv 2021-06-25T13:11:53Z
dc.date.available.none.fl_str_mv 2021-06-25T13:11:53Z
dc.type.spa.fl_str_mv info:eu-repo/semantics/article
dc.type.coarversion.fl_str_mv http://purl.org/coar/version/c_970fb48d4fbd8a85
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dc.type.local.spa.fl_str_mv Artículo de investigación
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dc.identifier.citation.spa.fl_str_mv : Gallego Rúa AM, Henao Ramírez AM, Urrea Trujillo AI, Atehortúa Garcés L. Polyphenols distributions and reserve substances analysis in cacao somatic embryogenesis. Acta biol. Colomb. 2016;21(2):335-345. doi: http://dx.doi.org/10.15446/abc.v21n2.50196
dc.identifier.issn.none.fl_str_mv 0120-548X
dc.identifier.uri.none.fl_str_mv http://hdl.handle.net/10495/20379
dc.identifier.doi.none.fl_str_mv 10.15446/abc.v21n2.50196
dc.identifier.eissn.none.fl_str_mv 1900-1649
identifier_str_mv : Gallego Rúa AM, Henao Ramírez AM, Urrea Trujillo AI, Atehortúa Garcés L. Polyphenols distributions and reserve substances analysis in cacao somatic embryogenesis. Acta biol. Colomb. 2016;21(2):335-345. doi: http://dx.doi.org/10.15446/abc.v21n2.50196
0120-548X
10.15446/abc.v21n2.50196
1900-1649
url http://hdl.handle.net/10495/20379
dc.language.iso.spa.fl_str_mv eng
language eng
dc.relation.ispartofjournalabbrev.spa.fl_str_mv Acta biol. Colomb.
dc.rights.spa.fl_str_mv info:eu-repo/semantics/openAccess
dc.rights.uri.*.fl_str_mv http://creativecommons.org/licenses/by-nc-sa/2.5/co/
dc.rights.accessrights.spa.fl_str_mv http://purl.org/coar/access_right/c_abf2
dc.rights.creativecommons.spa.fl_str_mv https://creativecommons.org/licenses/by-nc-sa/4.0/
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/2.5/co/
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dc.format.extent.spa.fl_str_mv 12
dc.format.mimetype.spa.fl_str_mv application/pdf
dc.publisher.spa.fl_str_mv Universidad Nacional de Colombia, Facultad de Ciencias, Departamento de Biología
dc.publisher.group.spa.fl_str_mv Biotecnología
dc.publisher.place.spa.fl_str_mv Bogotá, Colombia
institution Universidad de Antioquia
bitstream.url.fl_str_mv http://bibliotecadigital.udea.edu.co/bitstream/10495/20379/1/GallegoAdriana_2016_PolyphenolsDistributionCocoa.pdf
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spelling Gallego Rúa, Adriana MaríaHenao Ramírez, Ana MaríaUrrea Trujillo, Aura InésAtehortúa Garcés, Lucía2021-06-25T13:11:53Z2021-06-25T13:11:53Z2016: Gallego Rúa AM, Henao Ramírez AM, Urrea Trujillo AI, Atehortúa Garcés L. Polyphenols distributions and reserve substances analysis in cacao somatic embryogenesis. Acta biol. Colomb. 2016;21(2):335-345. doi: http://dx.doi.org/10.15446/abc.v21n2.501960120-548Xhttp://hdl.handle.net/10495/2037910.15446/abc.v21n2.501961900-1649ABSTRACT: In order to understand the causes of lack of regeneration in cacao somatic embryos, two cacao varieties with different responses to regeneration potential were described based on their capacity to store different compounds. It is well known that seed reserves play a central role in the regenerative capability of somatic embryos; thus, we followed histochemical changes and reserve fluctuations of proteins, polysaccharides and polyphenols during somatic embryogenesis (SE) in the two cacao varieties. The study showed that, in somatic embryos of the regenerating variety, polyphenols were localized mainly in the periphery of the embryo (epidermal cells) and proteins were the main storage substance in the embryo expression medium, while the non-regenerating variety had a high presence of polysaccharides with random distribution of polyphenols at the end of the embryo induction step.RESUMEN: Dos variedades de cacao con diferentes respuestas a la regeneración fueron descritas en función de su capacidad para almacenar diferentes compuestos, con el fin de aproximarse al entendimiento de las causas de la falta de regeneración en embriones somáticos de cacao. Es bien sabido que las reservas de semillas desempeñan un papel central en la capacidad de regeneración de embriones somáticos; por tanto, se realizó un seguimiento de cambios histoquímicos y fluctuaciones de reserva de proteínas, polisacáridos y polifenoles durante la embriogénesis somática (SE) en dos variedades de cacao. El estudio mostró que, en los embriones somáticos de la variedad regenerante, los polifenoles se localizaron principalmente en la periferia del embrión (células de la epidermis) y las proteínas fueron el componente principal de almacenamiento en el medio de expresión de embriones, mientras que la variedad no regenerante tenía una alta presencia de polisacáridos y una distribución aleatoria de los polifenoles en el final de la etapa de inducción de embriones.COL000108412application/pdfengUniversidad Nacional de Colombia, Facultad de Ciencias, Departamento de BiologíaBiotecnologíaBogotá, Colombiainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_2df8fbb1https://purl.org/redcol/resource_type/ARTArtículo de investigaciónhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/2.5/co/http://purl.org/coar/access_right/c_abf2https://creativecommons.org/licenses/by-nc-sa/4.0/Polyphenols distribution and reserve substances analysis in cocoa somatic embryogenesisAnálisis de distribución de polifenoles y sustancias de reserva en embriogénesis somática de cacaoAntioxidantsAntioxidantesHistologyHistologíaPlant Somatic Embryogenesis TechniquesTécnicas de Embriogénesis Somática de PlantasBanco de semillas del sueloSoil seed banksEmbriogénesis somáticaSomatic embryogenesisRecalcitranciaRecalcitrancehttp://aims.fao.org/aos/agrovoc/c_35298http://aims.fao.org/aos/agrovoc/c_36911Acta biol. Colomb.Acta Biológica Colombiana335345212ORIGINALGallegoAdriana_2016_PolyphenolsDistributionCocoa.pdfGallegoAdriana_2016_PolyphenolsDistributionCocoa.pdfArtículo de investigaciónapplication/pdf7687825http://bibliotecadigital.udea.edu.co/bitstream/10495/20379/1/GallegoAdriana_2016_PolyphenolsDistributionCocoa.pdff649b0ff0a5a1c92b8f9affe4d314a7fMD51CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-81051http://bibliotecadigital.udea.edu.co/bitstream/10495/20379/2/license_rdfe2060682c9c70d4d30c83c51448f4eedMD52LICENSElicense.txtlicense.txttext/plain; charset=utf-81748http://bibliotecadigital.udea.edu.co/bitstream/10495/20379/3/license.txt8a4605be74aa9ea9d79846c1fba20a33MD5310495/20379oai:bibliotecadigital.udea.edu.co:10495/203792021-06-25 08:12:26.801Repositorio Institucional Universidad de Antioquiaandres.perez@udea.edu.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