Comparative antioxidant, antiproliferative and apoptotic effects of Ilex laurina and Ilex paraguariensis on colon cancer cells
ABSTRACT: To determine and compare the antioxidant, antiproliferative and apoptotic effects of leaf infusions of Ilex laurina and Ilex paraguariensis in colon cancer cells. Methods: Antioxidant activity was determined by ORAC (Oxygen Radical Absorbance Capacity) and FRAP (Ferric Reducing Antioxidant...
- Autores:
-
Pérez Cardona, Juan Manuel
Maldonado Celis, María Elena
Rojano, Benjamín Alberto
Alzate Guarín, Fernando
Sáez Vega, Jairo Antonio
Cardona Galeano, Wilson
- Tipo de recurso:
- Article of investigation
- Fecha de publicación:
- 2014
- Institución:
- Universidad de Antioquia
- Repositorio:
- Repositorio UdeA
- Idioma:
- eng
- OAI Identifier:
- oai:bibliotecadigital.udea.edu.co:10495/12041
- Acceso en línea:
- http://hdl.handle.net/10495/12041
- Palabra clave:
- Ilex laurina
Ilex paraguariensis
Antioxidant
Antiproliferative
Apoptosis
Colon cancer
- Rights
- openAccess
- License
- https://creativecommons.org/licenses/by-nc-sa/2.5/co/
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| dc.title.spa.fl_str_mv |
Comparative antioxidant, antiproliferative and apoptotic effects of Ilex laurina and Ilex paraguariensis on colon cancer cells |
| title |
Comparative antioxidant, antiproliferative and apoptotic effects of Ilex laurina and Ilex paraguariensis on colon cancer cells |
| spellingShingle |
Comparative antioxidant, antiproliferative and apoptotic effects of Ilex laurina and Ilex paraguariensis on colon cancer cells Ilex laurina Ilex paraguariensis Antioxidant Antiproliferative Apoptosis Colon cancer |
| title_short |
Comparative antioxidant, antiproliferative and apoptotic effects of Ilex laurina and Ilex paraguariensis on colon cancer cells |
| title_full |
Comparative antioxidant, antiproliferative and apoptotic effects of Ilex laurina and Ilex paraguariensis on colon cancer cells |
| title_fullStr |
Comparative antioxidant, antiproliferative and apoptotic effects of Ilex laurina and Ilex paraguariensis on colon cancer cells |
| title_full_unstemmed |
Comparative antioxidant, antiproliferative and apoptotic effects of Ilex laurina and Ilex paraguariensis on colon cancer cells |
| title_sort |
Comparative antioxidant, antiproliferative and apoptotic effects of Ilex laurina and Ilex paraguariensis on colon cancer cells |
| dc.creator.fl_str_mv |
Pérez Cardona, Juan Manuel Maldonado Celis, María Elena Rojano, Benjamín Alberto Alzate Guarín, Fernando Sáez Vega, Jairo Antonio Cardona Galeano, Wilson |
| dc.contributor.author.none.fl_str_mv |
Pérez Cardona, Juan Manuel Maldonado Celis, María Elena Rojano, Benjamín Alberto Alzate Guarín, Fernando Sáez Vega, Jairo Antonio Cardona Galeano, Wilson |
| dc.contributor.researchgroup.spa.fl_str_mv |
Grupo de Estudios Botánicos Impacto de Componentes Alimentarios en la Salud Química de Plantas Colombianas |
| dc.subject.none.fl_str_mv |
Ilex laurina Ilex paraguariensis Antioxidant Antiproliferative Apoptosis Colon cancer |
| topic |
Ilex laurina Ilex paraguariensis Antioxidant Antiproliferative Apoptosis Colon cancer |
| description |
ABSTRACT: To determine and compare the antioxidant, antiproliferative and apoptotic effects of leaf infusions of Ilex laurina and Ilex paraguariensis in colon cancer cells. Methods: Antioxidant activity was determined by ORAC (Oxygen Radical Absorbance Capacity) and FRAP (Ferric Reducing Antioxidant Power). Cytotoxic and antiproliferative effects were analyzed using MTT ((3-(4, 5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide) and sulfhorodamine-B respectively. Cell death and apoptosis of human colon adenocarcinoma cells SW480 and their metastatic-derived SW620 cells, were analyzed by flow cytometry using propidium iodide and Annexin-V. Results: Although their flavonoid levels were similar, I. laurina infusion contained 2.2 and 4.4 times higher amounts of total phenolic and caffeoyl derivatives, respectively, than I. paraguariensis. FRAP and ORAC values for I. laurina infusion were 1.6 and 2.0 more active than I. paraguariensis. Both plant infusions inhibited viability and cell growth of SW480 and SW620 cells. These results may be associated to cell cycle-arrest and apoptosis because of the comparable increase of hypodiploid and annexin-V positive colon cancer cells. Conclusion: These data highlight the antioxidant and promising anticancer activities of I. laurina and Ilex paraguariensis. |
| publishDate |
2014 |
| dc.date.issued.none.fl_str_mv |
2014 |
| dc.date.accessioned.none.fl_str_mv |
2019-09-27T18:45:58Z |
| dc.date.available.none.fl_str_mv |
2019-09-27T18:45:58Z |
| dc.type.spa.fl_str_mv |
Artículo de investigación |
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http://purl.org/coar/resource_type/c_2df8fbb1 |
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https://purl.org/redcol/resource_type/ART |
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http://purl.org/coar/version/c_970fb48d4fbd8a85 |
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info:eu-repo/semantics/article |
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info:eu-repo/semantics/publishedVersion |
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http://purl.org/coar/resource_type/c_2df8fbb1 |
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publishedVersion |
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Pérez-Cardona J, Maldonado-Celis M, Rojano B, Alzate-Guarín F, Sáez-Vega J, Cardona-Galeano W. Comparative antioxidant, antiproliferative and apoptotic effects of Ilex laurina and Ilex paraguariensis on colon cancer cells. Trop J Pharm Res. 2014; 13(8): 1279-86. DOI: 10.4314/tjpr.v13i8.12 |
| dc.identifier.issn.none.fl_str_mv |
1596-5996 |
| dc.identifier.uri.none.fl_str_mv |
http://hdl.handle.net/10495/12041 |
| dc.identifier.doi.none.fl_str_mv |
10.4314/tjpr.v13i8.12 |
| dc.identifier.eissn.none.fl_str_mv |
1596-9827 |
| identifier_str_mv |
Pérez-Cardona J, Maldonado-Celis M, Rojano B, Alzate-Guarín F, Sáez-Vega J, Cardona-Galeano W. Comparative antioxidant, antiproliferative and apoptotic effects of Ilex laurina and Ilex paraguariensis on colon cancer cells. Trop J Pharm Res. 2014; 13(8): 1279-86. DOI: 10.4314/tjpr.v13i8.12 1596-5996 10.4314/tjpr.v13i8.12 1596-9827 |
| url |
http://hdl.handle.net/10495/12041 |
| dc.language.iso.spa.fl_str_mv |
eng |
| language |
eng |
| dc.relation.ispartofjournalabbrev.spa.fl_str_mv |
Trop. J. Pharm. Res. |
| dc.relation.citationendpage.spa.fl_str_mv |
1286 |
| dc.relation.citationissue.spa.fl_str_mv |
8 |
| dc.relation.citationstartpage.spa.fl_str_mv |
1279 |
| dc.relation.citationvolume.spa.fl_str_mv |
13 |
| dc.relation.ispartofjournal.spa.fl_str_mv |
Tropical Journal of Pharmaceutical Research |
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https://creativecommons.org/licenses/by-nc-sa/2.5/co/ |
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https://creativecommons.org/licenses/by-nc-sa/4.0/ |
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Atribución-NoComercial-CompartirIgual 2.5 Colombia (CC BY-NC-SA 2.5 CO) |
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info:eu-repo/semantics/openAccess |
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https://creativecommons.org/licenses/by-nc-sa/2.5/co/ https://creativecommons.org/licenses/by-nc-sa/4.0/ Atribución-NoComercial-CompartirIgual 2.5 Colombia (CC BY-NC-SA 2.5 CO) http://purl.org/coar/access_right/c_abf2 |
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openAccess |
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application/pdf |
| dc.publisher.spa.fl_str_mv |
University of Benin, Faculty of Pharmacy |
| dc.publisher.place.spa.fl_str_mv |
Ciudad de Benín, Nigeria |
| institution |
Universidad de Antioquia |
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Pérez Cardona, Juan ManuelMaldonado Celis, María ElenaRojano, Benjamín AlbertoAlzate Guarín, FernandoSáez Vega, Jairo AntonioCardona Galeano, WilsonGrupo de Estudios BotánicosImpacto de Componentes Alimentarios en la SaludQuímica de Plantas Colombianas2019-09-27T18:45:58Z2019-09-27T18:45:58Z2014Pérez-Cardona J, Maldonado-Celis M, Rojano B, Alzate-Guarín F, Sáez-Vega J, Cardona-Galeano W. Comparative antioxidant, antiproliferative and apoptotic effects of Ilex laurina and Ilex paraguariensis on colon cancer cells. Trop J Pharm Res. 2014; 13(8): 1279-86. DOI: 10.4314/tjpr.v13i8.121596-5996http://hdl.handle.net/10495/1204110.4314/tjpr.v13i8.121596-9827ABSTRACT: To determine and compare the antioxidant, antiproliferative and apoptotic effects of leaf infusions of Ilex laurina and Ilex paraguariensis in colon cancer cells. Methods: Antioxidant activity was determined by ORAC (Oxygen Radical Absorbance Capacity) and FRAP (Ferric Reducing Antioxidant Power). Cytotoxic and antiproliferative effects were analyzed using MTT ((3-(4, 5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide) and sulfhorodamine-B respectively. Cell death and apoptosis of human colon adenocarcinoma cells SW480 and their metastatic-derived SW620 cells, were analyzed by flow cytometry using propidium iodide and Annexin-V. Results: Although their flavonoid levels were similar, I. laurina infusion contained 2.2 and 4.4 times higher amounts of total phenolic and caffeoyl derivatives, respectively, than I. paraguariensis. FRAP and ORAC values for I. laurina infusion were 1.6 and 2.0 more active than I. paraguariensis. Both plant infusions inhibited viability and cell growth of SW480 and SW620 cells. These results may be associated to cell cycle-arrest and apoptosis because of the comparable increase of hypodiploid and annexin-V positive colon cancer cells. Conclusion: These data highlight the antioxidant and promising anticancer activities of I. laurina and Ilex paraguariensis.COL0008227COL0083811COL0015329application/pdfengUniversity of Benin, Faculty of PharmacyCiudad de Benín, Nigeriahttps://creativecommons.org/licenses/by-nc-sa/2.5/co/https://creativecommons.org/licenses/by-nc-sa/4.0/Atribución-NoComercial-CompartirIgual 2.5 Colombia (CC BY-NC-SA 2.5 CO)info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Ilex laurinaIlex paraguariensisAntioxidantAntiproliferativeApoptosisColon cancerComparative antioxidant, antiproliferative and apoptotic effects of Ilex laurina and Ilex paraguariensis on colon cancer cellsArtículo de investigaciónhttp://purl.org/coar/resource_type/c_2df8fbb1https://purl.org/redcol/resource_type/ARThttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionTrop. J. Pharm. 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