High genetic polymorphism of relapsing P. vivax isolates in northwest Colombia

ABSTRACT: Genetic diversity of Plasmodium populations has been more extensively documented in Colombia for Plasmodium falciparum than for Plasmodium vivax. Recently, highly variable microsatellite markers have been described and used in population-level studies of genetic variation of P. vivax throu...

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Autores:
Restrepo Pineda, Eliana
Imwong, Mallika
Rojas Montoya, Winston
Carmona Fonseca, Jaime
Maestre Buitrago, Amanda Elena
Tipo de recurso:
Article of investigation
Fecha de publicación:
2011
Institución:
Universidad de Antioquia
Repositorio:
Repositorio UdeA
Idioma:
eng
OAI Identifier:
oai:bibliotecadigital.udea.edu.co:10495/31168
Acceso en línea:
https://hdl.handle.net/10495/31168
Palabra clave:
Malaria
Plasmodium vivax
Polimorfismo Genético
Polymorphism, Genetic
Repeticiones de Microsatélite
Microsatellite Repeats
Recurrencia
Recurrence
Colombia
Rights
openAccess
License
http://creativecommons.org/licenses/by-nc-nd/2.5/co/
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oai_identifier_str oai:bibliotecadigital.udea.edu.co:10495/31168
network_acronym_str UDEA2
network_name_str Repositorio UdeA
repository_id_str
dc.title.spa.fl_str_mv High genetic polymorphism of relapsing P. vivax isolates in northwest Colombia
title High genetic polymorphism of relapsing P. vivax isolates in northwest Colombia
spellingShingle High genetic polymorphism of relapsing P. vivax isolates in northwest Colombia
Malaria
Plasmodium vivax
Polimorfismo Genético
Polymorphism, Genetic
Repeticiones de Microsatélite
Microsatellite Repeats
Recurrencia
Recurrence
Colombia
title_short High genetic polymorphism of relapsing P. vivax isolates in northwest Colombia
title_full High genetic polymorphism of relapsing P. vivax isolates in northwest Colombia
title_fullStr High genetic polymorphism of relapsing P. vivax isolates in northwest Colombia
title_full_unstemmed High genetic polymorphism of relapsing P. vivax isolates in northwest Colombia
title_sort High genetic polymorphism of relapsing P. vivax isolates in northwest Colombia
dc.creator.fl_str_mv Restrepo Pineda, Eliana
Imwong, Mallika
Rojas Montoya, Winston
Carmona Fonseca, Jaime
Maestre Buitrago, Amanda Elena
dc.contributor.author.none.fl_str_mv Restrepo Pineda, Eliana
Imwong, Mallika
Rojas Montoya, Winston
Carmona Fonseca, Jaime
Maestre Buitrago, Amanda Elena
dc.subject.decs.none.fl_str_mv Malaria
Plasmodium vivax
Polimorfismo Genético
Polymorphism, Genetic
Repeticiones de Microsatélite
Microsatellite Repeats
Recurrencia
Recurrence
Colombia
topic Malaria
Plasmodium vivax
Polimorfismo Genético
Polymorphism, Genetic
Repeticiones de Microsatélite
Microsatellite Repeats
Recurrencia
Recurrence
Colombia
description ABSTRACT: Genetic diversity of Plasmodium populations has been more extensively documented in Colombia for Plasmodium falciparum than for Plasmodium vivax. Recently, highly variable microsatellite markers have been described and used in population-level studies of genetic variation of P. vivax throughout the world. We applied this approach to understand the genetic structure of P. vivax populations and to identify recurrence-associated haplotypes. In this, three microsatellite markers of P. vivax were amplified and the combined size of the fragments was used to establish genotypes. Patients from an ongoing treatment efficacy trial who were kept either in endemic or non-endemic regions in the northwest of Colombia were included in the study. In total 58 paired clinical isolates, were amplified. A total of 54 haplotypes were observed among the two regions. Some haplotypes were exclusive to the endemic region where the highest degree of polymorphism was detected. In addition, we confirmed the different genotypes of recurrent-relapsing and primary infection isolates suggesting the activation of heterologous hypnozoite populations. We conclude that analysis of the three microsatellites is a valuable tool to establish the genetic characteristics of P. vivax populations in Colombia.
publishDate 2011
dc.date.issued.none.fl_str_mv 2011
dc.date.accessioned.none.fl_str_mv 2022-10-09T13:42:01Z
dc.date.available.none.fl_str_mv 2022-10-09T13:42:01Z
dc.type.spa.fl_str_mv info:eu-repo/semantics/article
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dc.type.local.spa.fl_str_mv Artículo de investigación
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dc.identifier.citation.spa.fl_str_mv Restrepo E, Imwong M, Rojas W, Carmona-Fonseca J, Maestre A. High genetic polymorphism of relapsing P. vivax isolates in northwest Colombia. Acta Trop. 2011 Jul;119(1):23-9. doi: 10.1016/j.actatropica.2011.03.012.
dc.identifier.issn.none.fl_str_mv 0001-706X
dc.identifier.uri.none.fl_str_mv https://hdl.handle.net/10495/31168
dc.identifier.doi.none.fl_str_mv 10.1016/j.actatropica.2011.03.012
dc.identifier.eissn.none.fl_str_mv 1873-6254
identifier_str_mv Restrepo E, Imwong M, Rojas W, Carmona-Fonseca J, Maestre A. High genetic polymorphism of relapsing P. vivax isolates in northwest Colombia. Acta Trop. 2011 Jul;119(1):23-9. doi: 10.1016/j.actatropica.2011.03.012.
0001-706X
10.1016/j.actatropica.2011.03.012
1873-6254
url https://hdl.handle.net/10495/31168
dc.language.iso.spa.fl_str_mv eng
language eng
dc.relation.ispartofjournalabbrev.spa.fl_str_mv Acta Tropica
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eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-nd/2.5/co/
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dc.format.extent.spa.fl_str_mv 7
dc.format.mimetype.spa.fl_str_mv application/pdf
dc.publisher.spa.fl_str_mv Elsevier
dc.publisher.group.spa.fl_str_mv Bacterias y Cáncer
Genética Molecular (GENMOL)
Salud y Comunidad
dc.publisher.place.spa.fl_str_mv Ámsterdam, Países Bajos
institution Universidad de Antioquia
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spelling Restrepo Pineda, ElianaImwong, MallikaRojas Montoya, WinstonCarmona Fonseca, JaimeMaestre Buitrago, Amanda Elena2022-10-09T13:42:01Z2022-10-09T13:42:01Z2011Restrepo E, Imwong M, Rojas W, Carmona-Fonseca J, Maestre A. High genetic polymorphism of relapsing P. vivax isolates in northwest Colombia. Acta Trop. 2011 Jul;119(1):23-9. doi: 10.1016/j.actatropica.2011.03.012.0001-706Xhttps://hdl.handle.net/10495/3116810.1016/j.actatropica.2011.03.0121873-6254ABSTRACT: Genetic diversity of Plasmodium populations has been more extensively documented in Colombia for Plasmodium falciparum than for Plasmodium vivax. Recently, highly variable microsatellite markers have been described and used in population-level studies of genetic variation of P. vivax throughout the world. We applied this approach to understand the genetic structure of P. vivax populations and to identify recurrence-associated haplotypes. In this, three microsatellite markers of P. vivax were amplified and the combined size of the fragments was used to establish genotypes. Patients from an ongoing treatment efficacy trial who were kept either in endemic or non-endemic regions in the northwest of Colombia were included in the study. In total 58 paired clinical isolates, were amplified. A total of 54 haplotypes were observed among the two regions. Some haplotypes were exclusive to the endemic region where the highest degree of polymorphism was detected. In addition, we confirmed the different genotypes of recurrent-relapsing and primary infection isolates suggesting the activation of heterologous hypnozoite populations. We conclude that analysis of the three microsatellites is a valuable tool to establish the genetic characteristics of P. vivax populations in Colombia.COL0070457COL0006723COL00474497application/pdfengElsevierBacterias y CáncerGenética Molecular (GENMOL)Salud y ComunidadÁmsterdam, Países Bajosinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_2df8fbb1https://purl.org/redcol/resource_type/ARTArtículo de investigaciónhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-nd/2.5/co/http://purl.org/coar/access_right/c_abf2https://creativecommons.org/licenses/by-nc-nd/4.0/High genetic polymorphism of relapsing P. vivax isolates in northwest ColombiaMalariaPlasmodium vivaxPolimorfismo GenéticoPolymorphism, GeneticRepeticiones de MicrosatéliteMicrosatellite RepeatsRecurrenciaRecurrenceColombiaActa TropicaActa Tropica2329119CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8823https://bibliotecadigital.udea.edu.co/bitstream/10495/31168/2/license_rdfb88b088d9957e670ce3b3fbe2eedbc13MD52LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://bibliotecadigital.udea.edu.co/bitstream/10495/31168/3/license.txt8a4605be74aa9ea9d79846c1fba20a33MD53ORIGINALRestrepoEliana_2011_MalariaPolymorphismColombia.pdfRestrepoEliana_2011_MalariaPolymorphismColombia.pdfArtículo de investigaciónapplication/pdf422326https://bibliotecadigital.udea.edu.co/bitstream/10495/31168/1/RestrepoEliana_2011_MalariaPolymorphismColombia.pdfe12ec5bb885bab01041dffb3dbc0010fMD5110495/31168oai:bibliotecadigital.udea.edu.co:10495/311682022-10-09 08:42:01.515Repositorio Institucional Universidad de Antioquiaandres.perez@udea.edu.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