High genetic polymorphism of relapsing P. vivax isolates in northwest Colombia
ABSTRACT: Genetic diversity of Plasmodium populations has been more extensively documented in Colombia for Plasmodium falciparum than for Plasmodium vivax. Recently, highly variable microsatellite markers have been described and used in population-level studies of genetic variation of P. vivax throu...
- Autores:
-
Restrepo Pineda, Eliana
Imwong, Mallika
Rojas Montoya, Winston
Carmona Fonseca, Jaime
Maestre Buitrago, Amanda Elena
- Tipo de recurso:
- Article of investigation
- Fecha de publicación:
- 2011
- Institución:
- Universidad de Antioquia
- Repositorio:
- Repositorio UdeA
- Idioma:
- eng
- OAI Identifier:
- oai:bibliotecadigital.udea.edu.co:10495/31168
- Acceso en línea:
- https://hdl.handle.net/10495/31168
- Palabra clave:
- Malaria
Plasmodium vivax
Polimorfismo Genético
Polymorphism, Genetic
Repeticiones de Microsatélite
Microsatellite Repeats
Recurrencia
Recurrence
Colombia
- Rights
- openAccess
- License
- http://creativecommons.org/licenses/by-nc-nd/2.5/co/
id |
UDEA2_9a17cdec17989da09d931205c67dc6d1 |
---|---|
oai_identifier_str |
oai:bibliotecadigital.udea.edu.co:10495/31168 |
network_acronym_str |
UDEA2 |
network_name_str |
Repositorio UdeA |
repository_id_str |
|
dc.title.spa.fl_str_mv |
High genetic polymorphism of relapsing P. vivax isolates in northwest Colombia |
title |
High genetic polymorphism of relapsing P. vivax isolates in northwest Colombia |
spellingShingle |
High genetic polymorphism of relapsing P. vivax isolates in northwest Colombia Malaria Plasmodium vivax Polimorfismo Genético Polymorphism, Genetic Repeticiones de Microsatélite Microsatellite Repeats Recurrencia Recurrence Colombia |
title_short |
High genetic polymorphism of relapsing P. vivax isolates in northwest Colombia |
title_full |
High genetic polymorphism of relapsing P. vivax isolates in northwest Colombia |
title_fullStr |
High genetic polymorphism of relapsing P. vivax isolates in northwest Colombia |
title_full_unstemmed |
High genetic polymorphism of relapsing P. vivax isolates in northwest Colombia |
title_sort |
High genetic polymorphism of relapsing P. vivax isolates in northwest Colombia |
dc.creator.fl_str_mv |
Restrepo Pineda, Eliana Imwong, Mallika Rojas Montoya, Winston Carmona Fonseca, Jaime Maestre Buitrago, Amanda Elena |
dc.contributor.author.none.fl_str_mv |
Restrepo Pineda, Eliana Imwong, Mallika Rojas Montoya, Winston Carmona Fonseca, Jaime Maestre Buitrago, Amanda Elena |
dc.subject.decs.none.fl_str_mv |
Malaria Plasmodium vivax Polimorfismo Genético Polymorphism, Genetic Repeticiones de Microsatélite Microsatellite Repeats Recurrencia Recurrence Colombia |
topic |
Malaria Plasmodium vivax Polimorfismo Genético Polymorphism, Genetic Repeticiones de Microsatélite Microsatellite Repeats Recurrencia Recurrence Colombia |
description |
ABSTRACT: Genetic diversity of Plasmodium populations has been more extensively documented in Colombia for Plasmodium falciparum than for Plasmodium vivax. Recently, highly variable microsatellite markers have been described and used in population-level studies of genetic variation of P. vivax throughout the world. We applied this approach to understand the genetic structure of P. vivax populations and to identify recurrence-associated haplotypes. In this, three microsatellite markers of P. vivax were amplified and the combined size of the fragments was used to establish genotypes. Patients from an ongoing treatment efficacy trial who were kept either in endemic or non-endemic regions in the northwest of Colombia were included in the study. In total 58 paired clinical isolates, were amplified. A total of 54 haplotypes were observed among the two regions. Some haplotypes were exclusive to the endemic region where the highest degree of polymorphism was detected. In addition, we confirmed the different genotypes of recurrent-relapsing and primary infection isolates suggesting the activation of heterologous hypnozoite populations. We conclude that analysis of the three microsatellites is a valuable tool to establish the genetic characteristics of P. vivax populations in Colombia. |
publishDate |
2011 |
dc.date.issued.none.fl_str_mv |
2011 |
dc.date.accessioned.none.fl_str_mv |
2022-10-09T13:42:01Z |
dc.date.available.none.fl_str_mv |
2022-10-09T13:42:01Z |
dc.type.spa.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.coarversion.fl_str_mv |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
dc.type.hasversion.spa.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.coar.spa.fl_str_mv |
http://purl.org/coar/resource_type/c_2df8fbb1 |
dc.type.redcol.spa.fl_str_mv |
https://purl.org/redcol/resource_type/ART |
dc.type.local.spa.fl_str_mv |
Artículo de investigación |
format |
http://purl.org/coar/resource_type/c_2df8fbb1 |
status_str |
publishedVersion |
dc.identifier.citation.spa.fl_str_mv |
Restrepo E, Imwong M, Rojas W, Carmona-Fonseca J, Maestre A. High genetic polymorphism of relapsing P. vivax isolates in northwest Colombia. Acta Trop. 2011 Jul;119(1):23-9. doi: 10.1016/j.actatropica.2011.03.012. |
dc.identifier.issn.none.fl_str_mv |
0001-706X |
dc.identifier.uri.none.fl_str_mv |
https://hdl.handle.net/10495/31168 |
dc.identifier.doi.none.fl_str_mv |
10.1016/j.actatropica.2011.03.012 |
dc.identifier.eissn.none.fl_str_mv |
1873-6254 |
identifier_str_mv |
Restrepo E, Imwong M, Rojas W, Carmona-Fonseca J, Maestre A. High genetic polymorphism of relapsing P. vivax isolates in northwest Colombia. Acta Trop. 2011 Jul;119(1):23-9. doi: 10.1016/j.actatropica.2011.03.012. 0001-706X 10.1016/j.actatropica.2011.03.012 1873-6254 |
url |
https://hdl.handle.net/10495/31168 |
dc.language.iso.spa.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartofjournalabbrev.spa.fl_str_mv |
Acta Tropica |
dc.rights.spa.fl_str_mv |
info:eu-repo/semantics/openAccess |
dc.rights.uri.*.fl_str_mv |
http://creativecommons.org/licenses/by-nc-nd/2.5/co/ |
dc.rights.accessrights.spa.fl_str_mv |
http://purl.org/coar/access_right/c_abf2 |
dc.rights.creativecommons.spa.fl_str_mv |
https://creativecommons.org/licenses/by-nc-nd/4.0/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by-nc-nd/2.5/co/ http://purl.org/coar/access_right/c_abf2 https://creativecommons.org/licenses/by-nc-nd/4.0/ |
dc.format.extent.spa.fl_str_mv |
7 |
dc.format.mimetype.spa.fl_str_mv |
application/pdf |
dc.publisher.spa.fl_str_mv |
Elsevier |
dc.publisher.group.spa.fl_str_mv |
Bacterias y Cáncer Genética Molecular (GENMOL) Salud y Comunidad |
dc.publisher.place.spa.fl_str_mv |
Ámsterdam, Países Bajos |
institution |
Universidad de Antioquia |
bitstream.url.fl_str_mv |
https://bibliotecadigital.udea.edu.co/bitstream/10495/31168/2/license_rdf https://bibliotecadigital.udea.edu.co/bitstream/10495/31168/3/license.txt https://bibliotecadigital.udea.edu.co/bitstream/10495/31168/1/RestrepoEliana_2011_MalariaPolymorphismColombia.pdf |
bitstream.checksum.fl_str_mv |
b88b088d9957e670ce3b3fbe2eedbc13 8a4605be74aa9ea9d79846c1fba20a33 e12ec5bb885bab01041dffb3dbc0010f |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 MD5 |
repository.name.fl_str_mv |
Repositorio Institucional Universidad de Antioquia |
repository.mail.fl_str_mv |
andres.perez@udea.edu.co |
_version_ |
1812173180904669184 |
spelling |
Restrepo Pineda, ElianaImwong, MallikaRojas Montoya, WinstonCarmona Fonseca, JaimeMaestre Buitrago, Amanda Elena2022-10-09T13:42:01Z2022-10-09T13:42:01Z2011Restrepo E, Imwong M, Rojas W, Carmona-Fonseca J, Maestre A. High genetic polymorphism of relapsing P. vivax isolates in northwest Colombia. Acta Trop. 2011 Jul;119(1):23-9. doi: 10.1016/j.actatropica.2011.03.012.0001-706Xhttps://hdl.handle.net/10495/3116810.1016/j.actatropica.2011.03.0121873-6254ABSTRACT: Genetic diversity of Plasmodium populations has been more extensively documented in Colombia for Plasmodium falciparum than for Plasmodium vivax. Recently, highly variable microsatellite markers have been described and used in population-level studies of genetic variation of P. vivax throughout the world. We applied this approach to understand the genetic structure of P. vivax populations and to identify recurrence-associated haplotypes. In this, three microsatellite markers of P. vivax were amplified and the combined size of the fragments was used to establish genotypes. Patients from an ongoing treatment efficacy trial who were kept either in endemic or non-endemic regions in the northwest of Colombia were included in the study. In total 58 paired clinical isolates, were amplified. A total of 54 haplotypes were observed among the two regions. Some haplotypes were exclusive to the endemic region where the highest degree of polymorphism was detected. In addition, we confirmed the different genotypes of recurrent-relapsing and primary infection isolates suggesting the activation of heterologous hypnozoite populations. We conclude that analysis of the three microsatellites is a valuable tool to establish the genetic characteristics of P. vivax populations in Colombia.COL0070457COL0006723COL00474497application/pdfengElsevierBacterias y CáncerGenética Molecular (GENMOL)Salud y ComunidadÁmsterdam, Países Bajosinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_2df8fbb1https://purl.org/redcol/resource_type/ARTArtículo de investigaciónhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-nd/2.5/co/http://purl.org/coar/access_right/c_abf2https://creativecommons.org/licenses/by-nc-nd/4.0/High genetic polymorphism of relapsing P. vivax isolates in northwest ColombiaMalariaPlasmodium vivaxPolimorfismo GenéticoPolymorphism, GeneticRepeticiones de MicrosatéliteMicrosatellite RepeatsRecurrenciaRecurrenceColombiaActa TropicaActa Tropica2329119CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8823https://bibliotecadigital.udea.edu.co/bitstream/10495/31168/2/license_rdfb88b088d9957e670ce3b3fbe2eedbc13MD52LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://bibliotecadigital.udea.edu.co/bitstream/10495/31168/3/license.txt8a4605be74aa9ea9d79846c1fba20a33MD53ORIGINALRestrepoEliana_2011_MalariaPolymorphismColombia.pdfRestrepoEliana_2011_MalariaPolymorphismColombia.pdfArtículo de investigaciónapplication/pdf422326https://bibliotecadigital.udea.edu.co/bitstream/10495/31168/1/RestrepoEliana_2011_MalariaPolymorphismColombia.pdfe12ec5bb885bab01041dffb3dbc0010fMD5110495/31168oai:bibliotecadigital.udea.edu.co:10495/311682022-10-09 08:42:01.515Repositorio Institucional Universidad de Antioquiaandres.perez@udea.edu.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 |