Injertos óseos : nueva alternativa. Fase I. Extracción de proteínas morfogenéticas parcialmente purificadas de hueso bovino

ABSTRACT: The purpose of this research was to extract from bovine bone and through physical and chemical procedures, Bone Morphogenetic Proteins (BMPs) partially purified, with high-quality osteoinduction characteristics, low antigenicity and high bioavailability. These proteins will be utilized as bone graft material. The procedure was performed through five basic steps: Milling the bovine bone, lipid extraction, demineralization, collagen extraction, obtention of the protein fraction. These steps were verified respectively through the following analysis: The Soxhlet method, Atomic Absorption for Calcium determination, Van Gieson Pycrofucsin Stain., Polyacrilamide Gel-Electrophoresis-Sodium- Duodecyl-Sulphate (PAGE-SDS). BMPs partially purified were obtained through Polyacrilamide Gel-Electrophoresis-Sodium-Duodecyl-Sulphate (PAGE-SDS., with molecular weights between 18 - 120 Kda, mainly bands at 18, 24, 34, 68, 72, 90 and 120 KDa, This material was lyophilized, packed and stored in a vacuum sealed narrow-neck bottle for posterior use.

• Autores: Rivera Posada, Jairo Alonso; Riaño Benavides, Carlos Humberto; Monsalve Cuartas, Paula Andrea; Osorio, Adriana
• Tipo de recurso: Article of investigation
• Fecha de publicación: 2003
• Institución: Universidad de Antioquia
• Repositorio: Repositorio UdeA
• Idioma: spa
• OAI Identifier: oai:bibliotecadigital.udea.edu.co:10495/7281
• Acceso en línea: http://hdl.handle.net/10495/7281
• Palabra clave: Proteínas morfogenéticas óseas; Huesos; Injerto óseo; Bovinos
• Rights: openAccess
• License: Atribución-NoComercial-CompartirIgual 2.5 Colombia (CC BY-NC-SA 2.5 CO)