Anti-Neurotoxins from Micrurus mipartitus in the Development of Coral Snake Antivenoms

ABSTRACT: In Colombia, the genus Micrurus includes 30 species, of which M. mipartitus and M. dumerilii are the most widely distributed. Micrurus causes less than 3% of the approximately 5000 cases of snakebite per year. The elapid envenomation caused by the snakes from the Micrurus genus, are charac...

Full description

Autores:
Cardona Ruda, Ana María
Rey Suárez, Jessica Paola
Núñez Rangel, Vitelbina
Tipo de recurso:
Article of investigation
Fecha de publicación:
2022
Institución:
Universidad de Antioquia
Repositorio:
Repositorio UdeA
Idioma:
eng
OAI Identifier:
oai:bibliotecadigital.udea.edu.co:10495/27726
Acceso en línea:
http://hdl.handle.net/10495/27726
Palabra clave:
Serpientes de Coral
Coral Snakes
Antivenenos
Antivenins
Receptores de Fosfolipasa A2
Receptors, Phospholipase A2
Anti-neurotoxinas
Anti-neurotoxins
Micrurus mipartitus
Rights
openAccess
License
Atribución 2.5 Colombia
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oai_identifier_str oai:bibliotecadigital.udea.edu.co:10495/27726
network_acronym_str UDEA2
network_name_str Repositorio UdeA
repository_id_str
dc.title.spa.fl_str_mv Anti-Neurotoxins from Micrurus mipartitus in the Development of Coral Snake Antivenoms
title Anti-Neurotoxins from Micrurus mipartitus in the Development of Coral Snake Antivenoms
spellingShingle Anti-Neurotoxins from Micrurus mipartitus in the Development of Coral Snake Antivenoms
Serpientes de Coral
Coral Snakes
Antivenenos
Antivenins
Receptores de Fosfolipasa A2
Receptors, Phospholipase A2
Anti-neurotoxinas
Anti-neurotoxins
Micrurus mipartitus
title_short Anti-Neurotoxins from Micrurus mipartitus in the Development of Coral Snake Antivenoms
title_full Anti-Neurotoxins from Micrurus mipartitus in the Development of Coral Snake Antivenoms
title_fullStr Anti-Neurotoxins from Micrurus mipartitus in the Development of Coral Snake Antivenoms
title_full_unstemmed Anti-Neurotoxins from Micrurus mipartitus in the Development of Coral Snake Antivenoms
title_sort Anti-Neurotoxins from Micrurus mipartitus in the Development of Coral Snake Antivenoms
dc.creator.fl_str_mv Cardona Ruda, Ana María
Rey Suárez, Jessica Paola
Núñez Rangel, Vitelbina
dc.contributor.author.none.fl_str_mv Cardona Ruda, Ana María
Rey Suárez, Jessica Paola
Núñez Rangel, Vitelbina
dc.subject.decs.none.fl_str_mv Serpientes de Coral
Coral Snakes
Antivenenos
Antivenins
Receptores de Fosfolipasa A2
Receptors, Phospholipase A2
topic Serpientes de Coral
Coral Snakes
Antivenenos
Antivenins
Receptores de Fosfolipasa A2
Receptors, Phospholipase A2
Anti-neurotoxinas
Anti-neurotoxins
Micrurus mipartitus
dc.subject.proposal.spa.fl_str_mv Anti-neurotoxinas
Anti-neurotoxins
Micrurus mipartitus
description ABSTRACT: In Colombia, the genus Micrurus includes 30 species, of which M. mipartitus and M. dumerilii are the most widely distributed. Micrurus causes less than 3% of the approximately 5000 cases of snakebite per year. The elapid envenomation caused by the snakes from the Micrurus genus, are characterized by the severity of their clinical manifestations, due to the venom neurotoxic components such as three-finger toxins (3FTx) and phospholipases (PLA2). The treatment for snakebites is the administration of specific antivenoms, however, some of them have limitations in their neutralizing ability. A strategy proposed to improve antivenoms is to produce antibodies against the main components of the venom. The aim of this work was to produce an antivenom, using an immunization protocol including the main 3FTx and PLA2 responsible for M. mipartitus lethality. The antibody titers were determined by ELISA in rabbits’ serum. The immunized animals elicited a response against toxins and whole venom. The Immunoglobulin G (IgGs) obtained were able to neutralize the lethal effect of their homologous toxins. A combination of antivenom from M. mipartitus with antitoxins improved their neutralizing ability. In the same way, a mixture of anti 3FTx and PLA2 protected the mice from a 1.5 median lethal dose (LD50) of M. mipartitus venom. The results showed that this might be a way to improve antibody titers specificity against the relevant toxins in M. mipartitus venom and indicated that there is a possibility to develop and use recombinant 3FTx and PLA2 toxins as immunogens to produce antivenoms. Additionally, this represents an alternative to reduce the amount of venom used in anti-coral antivenom production.
publishDate 2022
dc.date.accessioned.none.fl_str_mv 2022-04-22T21:51:06Z
dc.date.available.none.fl_str_mv 2022-04-22T21:51:06Z
dc.date.issued.none.fl_str_mv 2022
dc.type.spa.fl_str_mv info:eu-repo/semantics/article
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dc.type.redcol.spa.fl_str_mv https://purl.org/redcol/resource_type/ART
dc.type.local.spa.fl_str_mv Artículo de investigación
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dc.identifier.citation.spa.fl_str_mv Cardona-Ruda A, Rey-Suárez P, Núñez V. Anti-Neurotoxins from Micrurus mipartitus in the Development of Coral Snake Antivenoms. Toxins. 2022; 14(4):265. https://doi.org/10.3390/toxins14040265
dc.identifier.uri.none.fl_str_mv http://hdl.handle.net/10495/27726
dc.identifier.doi.none.fl_str_mv 10.3390/toxins14040265
dc.identifier.eissn.none.fl_str_mv 2072-6651
identifier_str_mv Cardona-Ruda A, Rey-Suárez P, Núñez V. Anti-Neurotoxins from Micrurus mipartitus in the Development of Coral Snake Antivenoms. Toxins. 2022; 14(4):265. https://doi.org/10.3390/toxins14040265
10.3390/toxins14040265
2072-6651
url http://hdl.handle.net/10495/27726
dc.language.iso.spa.fl_str_mv eng
language eng
dc.relation.ispartofjournalabbrev.spa.fl_str_mv Toxins
dc.rights.spa.fl_str_mv info:eu-repo/semantics/openAccess
dc.rights.*.fl_str_mv Atribución 2.5 Colombia
dc.rights.uri.*.fl_str_mv http://creativecommons.org/licenses/by/2.5/co/
dc.rights.accessrights.spa.fl_str_mv http://purl.org/coar/access_right/c_abf2
dc.rights.creativecommons.spa.fl_str_mv https://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
rights_invalid_str_mv Atribución 2.5 Colombia
http://creativecommons.org/licenses/by/2.5/co/
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by/4.0/
dc.format.extent.spa.fl_str_mv 10
dc.format.mimetype.spa.fl_str_mv application/pdf
dc.publisher.spa.fl_str_mv MDPI
dc.publisher.group.spa.fl_str_mv Toxinología Alternativas Terapéuticas y Alimentarias
dc.publisher.place.spa.fl_str_mv Basilea, Suiza
institution Universidad de Antioquia
bitstream.url.fl_str_mv http://bibliotecadigital.udea.edu.co/bitstream/10495/27726/2/license_rdf
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spelling Cardona Ruda, Ana MaríaRey Suárez, Jessica PaolaNúñez Rangel, Vitelbina2022-04-22T21:51:06Z2022-04-22T21:51:06Z2022Cardona-Ruda A, Rey-Suárez P, Núñez V. Anti-Neurotoxins from Micrurus mipartitus in the Development of Coral Snake Antivenoms. Toxins. 2022; 14(4):265. https://doi.org/10.3390/toxins14040265http://hdl.handle.net/10495/2772610.3390/toxins140402652072-6651ABSTRACT: In Colombia, the genus Micrurus includes 30 species, of which M. mipartitus and M. dumerilii are the most widely distributed. Micrurus causes less than 3% of the approximately 5000 cases of snakebite per year. The elapid envenomation caused by the snakes from the Micrurus genus, are characterized by the severity of their clinical manifestations, due to the venom neurotoxic components such as three-finger toxins (3FTx) and phospholipases (PLA2). The treatment for snakebites is the administration of specific antivenoms, however, some of them have limitations in their neutralizing ability. A strategy proposed to improve antivenoms is to produce antibodies against the main components of the venom. The aim of this work was to produce an antivenom, using an immunization protocol including the main 3FTx and PLA2 responsible for M. mipartitus lethality. The antibody titers were determined by ELISA in rabbits’ serum. The immunized animals elicited a response against toxins and whole venom. The Immunoglobulin G (IgGs) obtained were able to neutralize the lethal effect of their homologous toxins. A combination of antivenom from M. mipartitus with antitoxins improved their neutralizing ability. In the same way, a mixture of anti 3FTx and PLA2 protected the mice from a 1.5 median lethal dose (LD50) of M. mipartitus venom. The results showed that this might be a way to improve antibody titers specificity against the relevant toxins in M. mipartitus venom and indicated that there is a possibility to develop and use recombinant 3FTx and PLA2 toxins as immunogens to produce antivenoms. Additionally, this represents an alternative to reduce the amount of venom used in anti-coral antivenom production.COL001447610application/pdfengMDPIToxinología Alternativas Terapéuticas y AlimentariasBasilea, Suizainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_2df8fbb1https://purl.org/redcol/resource_type/ARTArtículo de investigaciónhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/openAccessAtribución 2.5 Colombiahttp://creativecommons.org/licenses/by/2.5/co/http://purl.org/coar/access_right/c_abf2https://creativecommons.org/licenses/by/4.0/Anti-Neurotoxins from Micrurus mipartitus in the Development of Coral Snake AntivenomsSerpientes de CoralCoral SnakesAntivenenosAntiveninsReceptores de Fosfolipasa A2Receptors, Phospholipase A2Anti-neurotoxinasAnti-neurotoxinsMicrurus mipartitusToxinsToxins110144CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8927http://bibliotecadigital.udea.edu.co/bitstream/10495/27726/2/license_rdf1646d1f6b96dbbbc38035efc9239ac9cMD52ORIGINALCardonaAna_2022_Anti-NeurotoxinsMicrurusMipartitus.pdfCardonaAna_2022_Anti-NeurotoxinsMicrurusMipartitus.pdfArtículo de investigaciónapplication/pdf3460570http://bibliotecadigital.udea.edu.co/bitstream/10495/27726/1/CardonaAna_2022_Anti-NeurotoxinsMicrurusMipartitus.pdf613d38222c4262faecec24b35b5314bcMD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748http://bibliotecadigital.udea.edu.co/bitstream/10495/27726/3/license.txt8a4605be74aa9ea9d79846c1fba20a33MD5310495/27726oai:bibliotecadigital.udea.edu.co:10495/277262022-04-22 16:51:07.66Repositorio Institucional Universidad de Antioquiaandres.perez@udea.edu.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