Anti-Neurotoxins from Micrurus mipartitus in the Development of Coral Snake Antivenoms
ABSTRACT: In Colombia, the genus Micrurus includes 30 species, of which M. mipartitus and M. dumerilii are the most widely distributed. Micrurus causes less than 3% of the approximately 5000 cases of snakebite per year. The elapid envenomation caused by the snakes from the Micrurus genus, are charac...
- Autores:
-
Cardona Ruda, Ana María
Rey Suárez, Jessica Paola
Núñez Rangel, Vitelbina
- Tipo de recurso:
- Article of investigation
- Fecha de publicación:
- 2022
- Institución:
- Universidad de Antioquia
- Repositorio:
- Repositorio UdeA
- Idioma:
- eng
- OAI Identifier:
- oai:bibliotecadigital.udea.edu.co:10495/27726
- Acceso en línea:
- http://hdl.handle.net/10495/27726
- Palabra clave:
- Serpientes de Coral
Coral Snakes
Antivenenos
Antivenins
Receptores de Fosfolipasa A2
Receptors, Phospholipase A2
Anti-neurotoxinas
Anti-neurotoxins
Micrurus mipartitus
- Rights
- openAccess
- License
- Atribución 2.5 Colombia
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|
dc.title.spa.fl_str_mv |
Anti-Neurotoxins from Micrurus mipartitus in the Development of Coral Snake Antivenoms |
title |
Anti-Neurotoxins from Micrurus mipartitus in the Development of Coral Snake Antivenoms |
spellingShingle |
Anti-Neurotoxins from Micrurus mipartitus in the Development of Coral Snake Antivenoms Serpientes de Coral Coral Snakes Antivenenos Antivenins Receptores de Fosfolipasa A2 Receptors, Phospholipase A2 Anti-neurotoxinas Anti-neurotoxins Micrurus mipartitus |
title_short |
Anti-Neurotoxins from Micrurus mipartitus in the Development of Coral Snake Antivenoms |
title_full |
Anti-Neurotoxins from Micrurus mipartitus in the Development of Coral Snake Antivenoms |
title_fullStr |
Anti-Neurotoxins from Micrurus mipartitus in the Development of Coral Snake Antivenoms |
title_full_unstemmed |
Anti-Neurotoxins from Micrurus mipartitus in the Development of Coral Snake Antivenoms |
title_sort |
Anti-Neurotoxins from Micrurus mipartitus in the Development of Coral Snake Antivenoms |
dc.creator.fl_str_mv |
Cardona Ruda, Ana María Rey Suárez, Jessica Paola Núñez Rangel, Vitelbina |
dc.contributor.author.none.fl_str_mv |
Cardona Ruda, Ana María Rey Suárez, Jessica Paola Núñez Rangel, Vitelbina |
dc.subject.decs.none.fl_str_mv |
Serpientes de Coral Coral Snakes Antivenenos Antivenins Receptores de Fosfolipasa A2 Receptors, Phospholipase A2 |
topic |
Serpientes de Coral Coral Snakes Antivenenos Antivenins Receptores de Fosfolipasa A2 Receptors, Phospholipase A2 Anti-neurotoxinas Anti-neurotoxins Micrurus mipartitus |
dc.subject.proposal.spa.fl_str_mv |
Anti-neurotoxinas Anti-neurotoxins Micrurus mipartitus |
description |
ABSTRACT: In Colombia, the genus Micrurus includes 30 species, of which M. mipartitus and M. dumerilii are the most widely distributed. Micrurus causes less than 3% of the approximately 5000 cases of snakebite per year. The elapid envenomation caused by the snakes from the Micrurus genus, are characterized by the severity of their clinical manifestations, due to the venom neurotoxic components such as three-finger toxins (3FTx) and phospholipases (PLA2). The treatment for snakebites is the administration of specific antivenoms, however, some of them have limitations in their neutralizing ability. A strategy proposed to improve antivenoms is to produce antibodies against the main components of the venom. The aim of this work was to produce an antivenom, using an immunization protocol including the main 3FTx and PLA2 responsible for M. mipartitus lethality. The antibody titers were determined by ELISA in rabbits’ serum. The immunized animals elicited a response against toxins and whole venom. The Immunoglobulin G (IgGs) obtained were able to neutralize the lethal effect of their homologous toxins. A combination of antivenom from M. mipartitus with antitoxins improved their neutralizing ability. In the same way, a mixture of anti 3FTx and PLA2 protected the mice from a 1.5 median lethal dose (LD50) of M. mipartitus venom. The results showed that this might be a way to improve antibody titers specificity against the relevant toxins in M. mipartitus venom and indicated that there is a possibility to develop and use recombinant 3FTx and PLA2 toxins as immunogens to produce antivenoms. Additionally, this represents an alternative to reduce the amount of venom used in anti-coral antivenom production. |
publishDate |
2022 |
dc.date.accessioned.none.fl_str_mv |
2022-04-22T21:51:06Z |
dc.date.available.none.fl_str_mv |
2022-04-22T21:51:06Z |
dc.date.issued.none.fl_str_mv |
2022 |
dc.type.spa.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.coarversion.fl_str_mv |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
dc.type.hasversion.spa.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.coar.spa.fl_str_mv |
http://purl.org/coar/resource_type/c_2df8fbb1 |
dc.type.redcol.spa.fl_str_mv |
https://purl.org/redcol/resource_type/ART |
dc.type.local.spa.fl_str_mv |
Artículo de investigación |
format |
http://purl.org/coar/resource_type/c_2df8fbb1 |
status_str |
publishedVersion |
dc.identifier.citation.spa.fl_str_mv |
Cardona-Ruda A, Rey-Suárez P, Núñez V. Anti-Neurotoxins from Micrurus mipartitus in the Development of Coral Snake Antivenoms. Toxins. 2022; 14(4):265. https://doi.org/10.3390/toxins14040265 |
dc.identifier.uri.none.fl_str_mv |
http://hdl.handle.net/10495/27726 |
dc.identifier.doi.none.fl_str_mv |
10.3390/toxins14040265 |
dc.identifier.eissn.none.fl_str_mv |
2072-6651 |
identifier_str_mv |
Cardona-Ruda A, Rey-Suárez P, Núñez V. Anti-Neurotoxins from Micrurus mipartitus in the Development of Coral Snake Antivenoms. Toxins. 2022; 14(4):265. https://doi.org/10.3390/toxins14040265 10.3390/toxins14040265 2072-6651 |
url |
http://hdl.handle.net/10495/27726 |
dc.language.iso.spa.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartofjournalabbrev.spa.fl_str_mv |
Toxins |
dc.rights.spa.fl_str_mv |
info:eu-repo/semantics/openAccess |
dc.rights.*.fl_str_mv |
Atribución 2.5 Colombia |
dc.rights.uri.*.fl_str_mv |
http://creativecommons.org/licenses/by/2.5/co/ |
dc.rights.accessrights.spa.fl_str_mv |
http://purl.org/coar/access_right/c_abf2 |
dc.rights.creativecommons.spa.fl_str_mv |
https://creativecommons.org/licenses/by/4.0/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
Atribución 2.5 Colombia http://creativecommons.org/licenses/by/2.5/co/ http://purl.org/coar/access_right/c_abf2 https://creativecommons.org/licenses/by/4.0/ |
dc.format.extent.spa.fl_str_mv |
10 |
dc.format.mimetype.spa.fl_str_mv |
application/pdf |
dc.publisher.spa.fl_str_mv |
MDPI |
dc.publisher.group.spa.fl_str_mv |
Toxinología Alternativas Terapéuticas y Alimentarias |
dc.publisher.place.spa.fl_str_mv |
Basilea, Suiza |
institution |
Universidad de Antioquia |
bitstream.url.fl_str_mv |
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MD5 MD5 MD5 |
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Repositorio Institucional Universidad de Antioquia |
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andres.perez@udea.edu.co |
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1812173103724232704 |
spelling |
Cardona Ruda, Ana MaríaRey Suárez, Jessica PaolaNúñez Rangel, Vitelbina2022-04-22T21:51:06Z2022-04-22T21:51:06Z2022Cardona-Ruda A, Rey-Suárez P, Núñez V. Anti-Neurotoxins from Micrurus mipartitus in the Development of Coral Snake Antivenoms. Toxins. 2022; 14(4):265. https://doi.org/10.3390/toxins14040265http://hdl.handle.net/10495/2772610.3390/toxins140402652072-6651ABSTRACT: In Colombia, the genus Micrurus includes 30 species, of which M. mipartitus and M. dumerilii are the most widely distributed. Micrurus causes less than 3% of the approximately 5000 cases of snakebite per year. The elapid envenomation caused by the snakes from the Micrurus genus, are characterized by the severity of their clinical manifestations, due to the venom neurotoxic components such as three-finger toxins (3FTx) and phospholipases (PLA2). The treatment for snakebites is the administration of specific antivenoms, however, some of them have limitations in their neutralizing ability. A strategy proposed to improve antivenoms is to produce antibodies against the main components of the venom. The aim of this work was to produce an antivenom, using an immunization protocol including the main 3FTx and PLA2 responsible for M. mipartitus lethality. The antibody titers were determined by ELISA in rabbits’ serum. The immunized animals elicited a response against toxins and whole venom. The Immunoglobulin G (IgGs) obtained were able to neutralize the lethal effect of their homologous toxins. A combination of antivenom from M. mipartitus with antitoxins improved their neutralizing ability. In the same way, a mixture of anti 3FTx and PLA2 protected the mice from a 1.5 median lethal dose (LD50) of M. mipartitus venom. The results showed that this might be a way to improve antibody titers specificity against the relevant toxins in M. mipartitus venom and indicated that there is a possibility to develop and use recombinant 3FTx and PLA2 toxins as immunogens to produce antivenoms. Additionally, this represents an alternative to reduce the amount of venom used in anti-coral antivenom production.COL001447610application/pdfengMDPIToxinología Alternativas Terapéuticas y AlimentariasBasilea, Suizainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_2df8fbb1https://purl.org/redcol/resource_type/ARTArtículo de investigaciónhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/openAccessAtribución 2.5 Colombiahttp://creativecommons.org/licenses/by/2.5/co/http://purl.org/coar/access_right/c_abf2https://creativecommons.org/licenses/by/4.0/Anti-Neurotoxins from Micrurus mipartitus in the Development of Coral Snake AntivenomsSerpientes de CoralCoral SnakesAntivenenosAntiveninsReceptores de Fosfolipasa A2Receptors, Phospholipase A2Anti-neurotoxinasAnti-neurotoxinsMicrurus mipartitusToxinsToxins110144CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8927http://bibliotecadigital.udea.edu.co/bitstream/10495/27726/2/license_rdf1646d1f6b96dbbbc38035efc9239ac9cMD52ORIGINALCardonaAna_2022_Anti-NeurotoxinsMicrurusMipartitus.pdfCardonaAna_2022_Anti-NeurotoxinsMicrurusMipartitus.pdfArtículo de investigaciónapplication/pdf3460570http://bibliotecadigital.udea.edu.co/bitstream/10495/27726/1/CardonaAna_2022_Anti-NeurotoxinsMicrurusMipartitus.pdf613d38222c4262faecec24b35b5314bcMD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748http://bibliotecadigital.udea.edu.co/bitstream/10495/27726/3/license.txt8a4605be74aa9ea9d79846c1fba20a33MD5310495/27726oai:bibliotecadigital.udea.edu.co:10495/277262022-04-22 16:51:07.66Repositorio Institucional Universidad de Antioquiaandres.perez@udea.edu.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 |