The Lymphocytes Stimulation Induced DNA Release, a Phenomenon Similar to NETosis

ABSTRACT: The release of DNA into the extracellular milieu by neutrophil during a process called NETosis has been postulated as an additional source of autoantigens; a process believed to be important in the pathogenesis of some autoimmune disease, such as systemic lupus erythematosus (SLE). However...

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Autores:
Rocha Arrieta, Yermis Carolina
Rojas López, Mauricio
Vásquez Duque, Gloria María
López Quintero, Juan Álvaro
Tipo de recurso:
Article of investigation
Fecha de publicación:
2017
Institución:
Universidad de Antioquia
Repositorio:
Repositorio UdeA
Idioma:
eng
OAI Identifier:
oai:bibliotecadigital.udea.edu.co:10495/23068
Acceso en línea:
http://hdl.handle.net/10495/23068
Palabra clave:
Linfocitos
Lymphocytes
Autoantígenos
Autoantigens
Rights
openAccess
License
http://creativecommons.org/licenses/by-nc-nd/2.5/co/
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oai_identifier_str oai:bibliotecadigital.udea.edu.co:10495/23068
network_acronym_str UDEA2
network_name_str Repositorio UdeA
repository_id_str
dc.title.spa.fl_str_mv The Lymphocytes Stimulation Induced DNA Release, a Phenomenon Similar to NETosis
title The Lymphocytes Stimulation Induced DNA Release, a Phenomenon Similar to NETosis
spellingShingle The Lymphocytes Stimulation Induced DNA Release, a Phenomenon Similar to NETosis
Linfocitos
Lymphocytes
Autoantígenos
Autoantigens
title_short The Lymphocytes Stimulation Induced DNA Release, a Phenomenon Similar to NETosis
title_full The Lymphocytes Stimulation Induced DNA Release, a Phenomenon Similar to NETosis
title_fullStr The Lymphocytes Stimulation Induced DNA Release, a Phenomenon Similar to NETosis
title_full_unstemmed The Lymphocytes Stimulation Induced DNA Release, a Phenomenon Similar to NETosis
title_sort The Lymphocytes Stimulation Induced DNA Release, a Phenomenon Similar to NETosis
dc.creator.fl_str_mv Rocha Arrieta, Yermis Carolina
Rojas López, Mauricio
Vásquez Duque, Gloria María
López Quintero, Juan Álvaro
dc.contributor.author.none.fl_str_mv Rocha Arrieta, Yermis Carolina
Rojas López, Mauricio
Vásquez Duque, Gloria María
López Quintero, Juan Álvaro
dc.subject.decs.none.fl_str_mv Linfocitos
Lymphocytes
Autoantígenos
Autoantigens
topic Linfocitos
Lymphocytes
Autoantígenos
Autoantigens
description ABSTRACT: The release of DNA into the extracellular milieu by neutrophil during a process called NETosis has been postulated as an additional source of autoantigens; a process believed to be important in the pathogenesis of some autoimmune disease, such as systemic lupus erythematosus (SLE). However, it is not established if the B and T cells undergo the release of DNA to the extracellular milleu, in response to different stimuli. In this study, it was observed that the treatment of B and T cells with PMA, ionomycin and the serum from patients with SLE induced the extracellular DNA presence in B and T cells. These findings suggest that the phenomenon were similar to those observed in neutrophil's Etosis; B and T cells also released their DNA into the extracellular milieu. The findings express that serum from patients with SLE and SLEDAI ≤ 8 triggers the release of extracellular DNA in neutrophils, B and T cells, that suggested the presence of soluble factors in the serum that favoured this phenomenon.
publishDate 2017
dc.date.issued.none.fl_str_mv 2017
dc.date.accessioned.none.fl_str_mv 2021-10-09T20:37:52Z
dc.date.available.none.fl_str_mv 2021-10-09T20:37:52Z
dc.type.spa.fl_str_mv info:eu-repo/semantics/article
dc.type.coarversion.fl_str_mv http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.hasversion.spa.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.coar.spa.fl_str_mv http://purl.org/coar/resource_type/c_2df8fbb1
dc.type.redcol.spa.fl_str_mv https://purl.org/redcol/resource_type/ART
dc.type.local.spa.fl_str_mv Artículo de investigación
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dc.identifier.issn.none.fl_str_mv 0300-9475
dc.identifier.uri.none.fl_str_mv http://hdl.handle.net/10495/23068
dc.identifier.doi.none.fl_str_mv 10.1111/sji.12592
dc.identifier.eissn.none.fl_str_mv 1365-3083
identifier_str_mv 0300-9475
10.1111/sji.12592
1365-3083
url http://hdl.handle.net/10495/23068
dc.language.iso.spa.fl_str_mv eng
language eng
dc.relation.ispartofjournalabbrev.spa.fl_str_mv Scand. J. Immunol.
dc.rights.spa.fl_str_mv info:eu-repo/semantics/openAccess
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dc.publisher.spa.fl_str_mv Wiley
dc.publisher.group.spa.fl_str_mv Grupo de Inmunología Celular e Inmunogenética
Inmunodeficiencias Primarias
dc.publisher.place.spa.fl_str_mv Oxford, Inglaterra
institution Universidad de Antioquia
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spelling Rocha Arrieta, Yermis CarolinaRojas López, MauricioVásquez Duque, Gloria MaríaLópez Quintero, Juan Álvaro2021-10-09T20:37:52Z2021-10-09T20:37:52Z20170300-9475http://hdl.handle.net/10495/2306810.1111/sji.125921365-3083ABSTRACT: The release of DNA into the extracellular milieu by neutrophil during a process called NETosis has been postulated as an additional source of autoantigens; a process believed to be important in the pathogenesis of some autoimmune disease, such as systemic lupus erythematosus (SLE). However, it is not established if the B and T cells undergo the release of DNA to the extracellular milleu, in response to different stimuli. In this study, it was observed that the treatment of B and T cells with PMA, ionomycin and the serum from patients with SLE induced the extracellular DNA presence in B and T cells. These findings suggest that the phenomenon were similar to those observed in neutrophil's Etosis; B and T cells also released their DNA into the extracellular milieu. The findings express that serum from patients with SLE and SLEDAI ≤ 8 triggers the release of extracellular DNA in neutrophils, B and T cells, that suggested the presence of soluble factors in the serum that favoured this phenomenon.COL0008639COL001242610application/pdfengWileyGrupo de Inmunología Celular e InmunogenéticaInmunodeficiencias PrimariasOxford, Inglaterrainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_2df8fbb1https://purl.org/redcol/resource_type/ARTArtículo de investigaciónhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-nd/2.5/co/http://purl.org/coar/access_right/c_abf2https://creativecommons.org/licenses/by-nc-nd/4.0/The Lymphocytes Stimulation Induced DNA Release, a Phenomenon Similar to NETosisLinfocitosLymphocytesAutoantígenosAutoantigensScand. J. Immunol.Scandinavian Journal of Immunology22923886LICENSElicense.txtlicense.txttext/plain; charset=utf-81748http://bibliotecadigital.udea.edu.co/bitstream/10495/23068/3/license.txt8a4605be74aa9ea9d79846c1fba20a33MD53CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8823http://bibliotecadigital.udea.edu.co/bitstream/10495/23068/2/license_rdfb88b088d9957e670ce3b3fbe2eedbc13MD52ORIGINALRochaYermis_2017_LymphocytesStimulationInduced.pdfRochaYermis_2017_LymphocytesStimulationInduced.pdfArtículo de investigaciónapplication/pdf2035534http://bibliotecadigital.udea.edu.co/bitstream/10495/23068/1/RochaYermis_2017_LymphocytesStimulationInduced.pdf978f7147a8cd18a09e2643e4fc7c5cc7MD5110495/23068oai:bibliotecadigital.udea.edu.co:10495/230682021-10-09 15:37:53.232Repositorio Institucional Universidad de Antioquiaandres.perez@udea.edu.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