Detection of macrophages infected with Mycobacterium avium subspecies paratuberculosis in a cow with clinical stage IV of Johne's disease. A case report
ABSTRACT: Anamnesis: a six-year-old milking Holstein cow (Bos taurus taurus) was diagnosed with stage IV Johne´s Disease (JD). Clinical and Laboratory findings: the cow suffered intermittent diarrhea during 6 months with no response to antibiotic treatment. Consequently, the cow was subjected to eut...
- Autores:
-
Ramírez García, René
Maldonado Estrada, Juan Guillermo
- Tipo de recurso:
- Article of journal
- Fecha de publicación:
- 2013
- Institución:
- Universidad de Antioquia
- Repositorio:
- Repositorio UdeA
- Idioma:
- eng
- OAI Identifier:
- oai:bibliotecadigital.udea.edu.co:10495/8067
- Acceso en línea:
- http://hdl.handle.net/10495/8067
- Palabra clave:
- Intracellular bacteria
Real time PCR
Resident macrophages
Susceptible host
Mycobacterium avium
Paratuberculosis
PCR (Reacción en cadena de la polimerasa)
Macrófagos
- Rights
- openAccess
- License
- Atribución-NoComercial-CompartirIgual 2.5 Colombia (CC BY-NC-SA 2.5 CO)
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Detection of macrophages infected with Mycobacterium avium subspecies paratuberculosis in a cow with clinical stage IV of Johne's disease. A case report |
| dc.title.alternative.spa.fl_str_mv |
Detección de macrófagos infectados con Mycobacterium avium subspecies paratuberculosis en una vaca en fase clínica IV de la enfermedad de Johne. Reporte de un caso |
| title |
Detection of macrophages infected with Mycobacterium avium subspecies paratuberculosis in a cow with clinical stage IV of Johne's disease. A case report |
| spellingShingle |
Detection of macrophages infected with Mycobacterium avium subspecies paratuberculosis in a cow with clinical stage IV of Johne's disease. A case report Intracellular bacteria Real time PCR Resident macrophages Susceptible host Mycobacterium avium Paratuberculosis PCR (Reacción en cadena de la polimerasa) Macrófagos |
| title_short |
Detection of macrophages infected with Mycobacterium avium subspecies paratuberculosis in a cow with clinical stage IV of Johne's disease. A case report |
| title_full |
Detection of macrophages infected with Mycobacterium avium subspecies paratuberculosis in a cow with clinical stage IV of Johne's disease. A case report |
| title_fullStr |
Detection of macrophages infected with Mycobacterium avium subspecies paratuberculosis in a cow with clinical stage IV of Johne's disease. A case report |
| title_full_unstemmed |
Detection of macrophages infected with Mycobacterium avium subspecies paratuberculosis in a cow with clinical stage IV of Johne's disease. A case report |
| title_sort |
Detection of macrophages infected with Mycobacterium avium subspecies paratuberculosis in a cow with clinical stage IV of Johne's disease. A case report |
| dc.creator.fl_str_mv |
Ramírez García, René Maldonado Estrada, Juan Guillermo |
| dc.contributor.author.none.fl_str_mv |
Ramírez García, René Maldonado Estrada, Juan Guillermo |
| dc.subject.none.fl_str_mv |
Intracellular bacteria Real time PCR Resident macrophages Susceptible host Mycobacterium avium Paratuberculosis PCR (Reacción en cadena de la polimerasa) Macrófagos |
| topic |
Intracellular bacteria Real time PCR Resident macrophages Susceptible host Mycobacterium avium Paratuberculosis PCR (Reacción en cadena de la polimerasa) Macrófagos |
| description |
ABSTRACT: Anamnesis: a six-year-old milking Holstein cow (Bos taurus taurus) was diagnosed with stage IV Johne´s Disease (JD). Clinical and Laboratory findings: the cow suffered intermittent diarrhea during 6 months with no response to antibiotic treatment. Consequently, the cow was subjected to euthanasia. Treatment approach: antemortem milk and peripheral blood samples and postmortem colon, mediastinic, mesenteric lymph nodes, and spleen samples were processed for macrophages isolation. Total DNA was extracted from macrophages and used to diagnose IS900 of Mycobacterium avium paratuberculosis (MAP) through real time PCR. The MAP IS900 segment was successfully amplified from cells of all samples, indicating that these cells were MAP-infected macrophages. Conclusion: macrophages of cows suffering from JD can be used for amplification of the MAP IS900 segment. |
| publishDate |
2013 |
| dc.date.issued.none.fl_str_mv |
2013 |
| dc.date.accessioned.none.fl_str_mv |
2017-08-30T18:41:57Z |
| dc.date.available.none.fl_str_mv |
2017-08-30T18:41:57Z |
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info:eu-repo/semantics/article |
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http://purl.org/coar/resource_type/c_2df8fbb1 |
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http://purl.org/coar/version/c_970fb48d4fbd8a85 |
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info:eu-repo/semantics/publishedVersion |
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http://purl.org/coar/resource_type/c_6501 |
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https://purl.org/redcol/resource_type/ARTCASO |
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Reporte de caso |
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http://purl.org/coar/resource_type/c_6501 |
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publishedVersion |
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Ramírez García R, Maldonado Estrada JG. Detection of macrophages infected with Mycobacterium avium subspecies paratuberculosis in a cow with clinical stage IV of Johne's disease. A case report. Rev. colomb. cienc. pecu. 2013 Sep; 26(3): 219-225 |
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0120-0690 |
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http://hdl.handle.net/10495/8067 |
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2256-2958 |
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Ramírez García R, Maldonado Estrada JG. Detection of macrophages infected with Mycobacterium avium subspecies paratuberculosis in a cow with clinical stage IV of Johne's disease. A case report. Rev. colomb. cienc. pecu. 2013 Sep; 26(3): 219-225 0120-0690 2256-2958 |
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http://hdl.handle.net/10495/8067 |
| dc.language.iso.spa.fl_str_mv |
eng |
| language |
eng |
| dc.relation.ispartofjournalabbrev.spa.fl_str_mv |
Rev. Colomb. Cienc. Pecu. |
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Atribución-NoComercial-CompartirIgual 2.5 Colombia (CC BY-NC-SA 2.5 CO) |
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info:eu-repo/semantics/openAccess |
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Universidad de Antioquia, Facultad de Ciencias Agrarias |
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CENTAURO |
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Medellín, Colombia |
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Universidad de Antioquia |
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Ramírez García, RenéMaldonado Estrada, Juan Guillermo2017-08-30T18:41:57Z2017-08-30T18:41:57Z2013Ramírez García R, Maldonado Estrada JG. Detection of macrophages infected with Mycobacterium avium subspecies paratuberculosis in a cow with clinical stage IV of Johne's disease. A case report. Rev. colomb. cienc. pecu. 2013 Sep; 26(3): 219-2250120-0690http://hdl.handle.net/10495/80672256-2958ABSTRACT: Anamnesis: a six-year-old milking Holstein cow (Bos taurus taurus) was diagnosed with stage IV Johne´s Disease (JD). Clinical and Laboratory findings: the cow suffered intermittent diarrhea during 6 months with no response to antibiotic treatment. Consequently, the cow was subjected to euthanasia. Treatment approach: antemortem milk and peripheral blood samples and postmortem colon, mediastinic, mesenteric lymph nodes, and spleen samples were processed for macrophages isolation. Total DNA was extracted from macrophages and used to diagnose IS900 of Mycobacterium avium paratuberculosis (MAP) through real time PCR. The MAP IS900 segment was successfully amplified from cells of all samples, indicating that these cells were MAP-infected macrophages. Conclusion: macrophages of cows suffering from JD can be used for amplification of the MAP IS900 segment.RESUMEN: una vaca Holstein de 6 años de edad (Bos taurus taurus) presentó sintomatología de la fase IV de la enfermedad de Johne. Hallazgos clínicos y de laboratorio: la vaca presentó diarrea intermitente durante 6 meses sin respuesta al tratamiento con antibióticos. En consecuencia, la vaca fue sometida a eutanasia. Esquema de tratamiento: muestras de leche y de sangre periférica se tomaron ante-mortem; muestras de la mucosa del intestino, bazo y linfonodos mediastínico y mesentérico, se tomaron post-mortem, todas para aislamiento de macrófagos. El ADN total de los macrófagos fue usado para la amplificación del segmento IS900 de Mycobacterium avium paratuberculosis (MAP) por PCR en tiempo real. Conclusión: los macrófagos de vacas con la enfermedad de Johne pueden ser usados para la amplificación del segmento IS900 de MAP.COL0001262application/pdfengUniversidad de Antioquia, Facultad de Ciencias AgrariasCENTAUROMedellín, Colombiainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1https://purl.org/redcol/resource_type/ARTCASOReporte de casohttp://purl.org/coar/version/c_970fb48d4fbd8a85Atribución-NoComercial-CompartirIgual 2.5 Colombia (CC BY-NC-SA 2.5 CO)info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/co/http://purl.org/coar/access_right/c_abf2https://creativecommons.org/licenses/by-nc-sa/4.0/Intracellular bacteriaReal time PCRResident macrophagesSusceptible hostMycobacterium aviumParatuberculosisPCR (Reacción en cadena de la polimerasa)MacrófagosDetection of macrophages infected with Mycobacterium avium subspecies paratuberculosis in a cow with clinical stage IV of Johne's disease. A case reportDetección de macrófagos infectados con Mycobacterium avium subspecies paratuberculosis en una vaca en fase clínica IV de la enfermedad de Johne. Reporte de un casoRev. Colomb. Cienc. Pecu.Revista Colombiana de Ciencias Pecuarias219225263ORIGINALRamirezRene_2013_DetectionMacrophagesInfected.pdfRamirezRene_2013_DetectionMacrophagesInfected.pdfReporte de casoapplication/pdf786619http://bibliotecadigital.udea.edu.co/bitstream/10495/8067/1/RamirezRene_2013_DetectionMacrophagesInfected.pdf8a8a4615878964be0360c2cde6317691MD51CC-LICENSElicense_urllicense_urltext/plain; charset=utf-849http://bibliotecadigital.udea.edu.co/bitstream/10495/8067/2/license_url4afdbb8c545fd630ea7db775da747b2fMD52license_textlicense_texttext/html; charset=utf-80http://bibliotecadigital.udea.edu.co/bitstream/10495/8067/3/license_textd41d8cd98f00b204e9800998ecf8427eMD53license_rdflicense_rdfapplication/rdf+xml; charset=utf-80http://bibliotecadigital.udea.edu.co/bitstream/10495/8067/4/license_rdfd41d8cd98f00b204e9800998ecf8427eMD54LICENSElicense.txtlicense.txttext/plain; charset=utf-81748http://bibliotecadigital.udea.edu.co/bitstream/10495/8067/5/license.txt8a4605be74aa9ea9d79846c1fba20a33MD5510495/8067oai:bibliotecadigital.udea.edu.co:10495/80672021-05-21 20:28:38.3Repositorio Institucional Universidad de Antioquiaandres.perez@udea.edu.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 |