Anticoagulant properties of a high molecular weight polysaccharide fraction (1000RS) of the ascidian Microcosmus exasperatus
ABSTRACT: The anticoagulant effect and cytotoxicity of a high molecular weight polysaccharide fraction (1000RS) obtained from the tunic of the ascidia Microcosmus exasperatus were evaluated. Methods: Anticoagulant properties of 1000RS was evaluated by activated Partial Thromboplastin Time (aPTT), Th...
- Autores:
-
Restrepo Espinosa, Diana Carolina
Maria Motta, Juliana
Castro O. de B. Teixeira, Felipe
Román, Yony
Colorado Ríos, Jhonny
S. G. Pavão, Mauro
Martínez Martínez, Alejandro
- Tipo de recurso:
- Article of investigation
- Fecha de publicación:
- 2017
- Institución:
- Universidad de Antioquia
- Repositorio:
- Repositorio UdeA
- Idioma:
- eng
- OAI Identifier:
- oai:bibliotecadigital.udea.edu.co:10495/13094
- Acceso en línea:
- http://hdl.handle.net/10495/13094
- Palabra clave:
- Anticoagulantes
Anticoagulants
Polisacáridos
Polysaccharides
Ascidia
Sea squirts
Microcosmos exasperatus
http://aims.fao.org/aos/agrovoc/c_6900
- Rights
- openAccess
- License
- Atribución-NoComercial-CompartirIgual 2.5 Colombia (CC BY-NC-SA 2.5 CO)
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| dc.title.spa.fl_str_mv |
Anticoagulant properties of a high molecular weight polysaccharide fraction (1000RS) of the ascidian Microcosmus exasperatus |
| dc.title.alternative.spa.fl_str_mv |
Propiedades anticoagulantes de una fracción polisacárida de alto peso molecular (1000RS) del ascidian Microcosmus exasperatus |
| title |
Anticoagulant properties of a high molecular weight polysaccharide fraction (1000RS) of the ascidian Microcosmus exasperatus |
| spellingShingle |
Anticoagulant properties of a high molecular weight polysaccharide fraction (1000RS) of the ascidian Microcosmus exasperatus Anticoagulantes Anticoagulants Polisacáridos Polysaccharides Ascidia Sea squirts Microcosmos exasperatus http://aims.fao.org/aos/agrovoc/c_6900 |
| title_short |
Anticoagulant properties of a high molecular weight polysaccharide fraction (1000RS) of the ascidian Microcosmus exasperatus |
| title_full |
Anticoagulant properties of a high molecular weight polysaccharide fraction (1000RS) of the ascidian Microcosmus exasperatus |
| title_fullStr |
Anticoagulant properties of a high molecular weight polysaccharide fraction (1000RS) of the ascidian Microcosmus exasperatus |
| title_full_unstemmed |
Anticoagulant properties of a high molecular weight polysaccharide fraction (1000RS) of the ascidian Microcosmus exasperatus |
| title_sort |
Anticoagulant properties of a high molecular weight polysaccharide fraction (1000RS) of the ascidian Microcosmus exasperatus |
| dc.creator.fl_str_mv |
Restrepo Espinosa, Diana Carolina Maria Motta, Juliana Castro O. de B. Teixeira, Felipe Román, Yony Colorado Ríos, Jhonny S. G. Pavão, Mauro Martínez Martínez, Alejandro |
| dc.contributor.author.none.fl_str_mv |
Restrepo Espinosa, Diana Carolina Maria Motta, Juliana Castro O. de B. Teixeira, Felipe Román, Yony Colorado Ríos, Jhonny S. G. Pavão, Mauro Martínez Martínez, Alejandro |
| dc.subject.decs.none.fl_str_mv |
Anticoagulantes Anticoagulants |
| topic |
Anticoagulantes Anticoagulants Polisacáridos Polysaccharides Ascidia Sea squirts Microcosmos exasperatus http://aims.fao.org/aos/agrovoc/c_6900 |
| dc.subject.lemb.none.fl_str_mv |
Polisacáridos Polysaccharides |
| dc.subject.agrovoc.none.fl_str_mv |
Ascidia Sea squirts |
| dc.subject.proposal.spa.fl_str_mv |
Microcosmos exasperatus |
| dc.subject.agrovocuri.none.fl_str_mv |
http://aims.fao.org/aos/agrovoc/c_6900 |
| description |
ABSTRACT: The anticoagulant effect and cytotoxicity of a high molecular weight polysaccharide fraction (1000RS) obtained from the tunic of the ascidia Microcosmus exasperatus were evaluated. Methods: Anticoagulant properties of 1000RS was evaluated by activated Partial Thromboplastin Time (aPTT), Thrombin Time (TT), Prothrombin Time (PT), anti-factor Xa and lupic anticoagulant (dRVVT) assays. Cytotoxicity was tested on murine hematopoietic cells using MTT assay. Results: This galactose rich fraction showed to be a potential anticoagulant due to its inhibitory effect on the intrinsic coagulation pathway. At the same time, anticoagulant doses of this fraction have no effect on cellular viability, which means that it can be used as a therapeutic agent. Conclusion: In vitro anticoagulant effect of 1000RS occurs at innocuous doses, however, it still need to be tested using in vivo models and its cytotoxicity evaluated in normal human cell lines. |
| publishDate |
2017 |
| dc.date.issued.none.fl_str_mv |
2017 |
| dc.date.accessioned.none.fl_str_mv |
2020-01-13T21:08:23Z |
| dc.date.available.none.fl_str_mv |
2020-01-13T21:08:23Z |
| dc.type.spa.fl_str_mv |
info:eu-repo/semantics/article |
| dc.type.coarversion.fl_str_mv |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| dc.type.hasversion.spa.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
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http://purl.org/coar/resource_type/c_2df8fbb1 |
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https://purl.org/redcol/resource_type/ART |
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Artículo de investigación |
| format |
http://purl.org/coar/resource_type/c_2df8fbb1 |
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publishedVersion |
| dc.identifier.citation.spa.fl_str_mv |
Restrepo Espinosa, D. A., Maria Motta, J., Castro O. de B. Teixeira, Román, Y., Colorado Ríos, J., S. G. Pavão, M., & Martínez Martínez, A. (2017). Anticoagulant properties of a high molecular weight polysaccharide fraction (1000RS) of the ascidian Microcosmus exasperatus. Ars Pharmaceutica, 58(3), 95-101. |
| dc.identifier.issn.none.fl_str_mv |
0004-2927 |
| dc.identifier.uri.none.fl_str_mv |
http://hdl.handle.net/10495/13094 |
| dc.identifier.doi.none.fl_str_mv |
10.4321/S2340-98942017000300002 |
| dc.identifier.eissn.none.fl_str_mv |
2340-9894 |
| identifier_str_mv |
Restrepo Espinosa, D. A., Maria Motta, J., Castro O. de B. Teixeira, Román, Y., Colorado Ríos, J., S. G. Pavão, M., & Martínez Martínez, A. (2017). Anticoagulant properties of a high molecular weight polysaccharide fraction (1000RS) of the ascidian Microcosmus exasperatus. Ars Pharmaceutica, 58(3), 95-101. 0004-2927 10.4321/S2340-98942017000300002 2340-9894 |
| url |
http://hdl.handle.net/10495/13094 |
| dc.language.iso.spa.fl_str_mv |
eng |
| language |
eng |
| dc.rights.*.fl_str_mv |
Atribución-NoComercial-CompartirIgual 2.5 Colombia (CC BY-NC-SA 2.5 CO) |
| dc.rights.spa.fl_str_mv |
info:eu-repo/semantics/openAccess |
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https://creativecommons.org/licenses/by-nc-sa/2.5/co/ |
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http://purl.org/coar/access_right/c_abf2 |
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https://creativecommons.org/licenses/by-nc-sa/4.0/ |
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Atribución-NoComercial-CompartirIgual 2.5 Colombia (CC BY-NC-SA 2.5 CO) https://creativecommons.org/licenses/by-nc-sa/2.5/co/ http://purl.org/coar/access_right/c_abf2 https://creativecommons.org/licenses/by-nc-sa/4.0/ |
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openAccess |
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application/pdf |
| dc.publisher.spa.fl_str_mv |
Facultad de Farmacia, Universidad de Granada |
| dc.publisher.group.spa.fl_str_mv |
Productos Naturales Marinos |
| dc.publisher.place.spa.fl_str_mv |
España |
| institution |
Universidad de Antioquia |
| bitstream.url.fl_str_mv |
https://bibliotecadigital.udea.edu.co/bitstream/10495/13094/2/license_url https://bibliotecadigital.udea.edu.co/bitstream/10495/13094/3/license_text https://bibliotecadigital.udea.edu.co/bitstream/10495/13094/4/license_rdf https://bibliotecadigital.udea.edu.co/bitstream/10495/13094/5/license.txt https://bibliotecadigital.udea.edu.co/bitstream/10495/13094/1/MartinezAlejandro_2017_AnticoagulantPropertiesMolecular.pdf |
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andres.perez@udea.edu.co |
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1812173300720205824 |
| spelling |
Restrepo Espinosa, Diana CarolinaMaria Motta, JulianaCastro O. de B. Teixeira, FelipeRomán, YonyColorado Ríos, JhonnyS. G. Pavão, MauroMartínez Martínez, Alejandro2020-01-13T21:08:23Z2020-01-13T21:08:23Z2017Restrepo Espinosa, D. A., Maria Motta, J., Castro O. de B. Teixeira, Román, Y., Colorado Ríos, J., S. G. Pavão, M., & Martínez Martínez, A. (2017). Anticoagulant properties of a high molecular weight polysaccharide fraction (1000RS) of the ascidian Microcosmus exasperatus. Ars Pharmaceutica, 58(3), 95-101.0004-2927http://hdl.handle.net/10495/1309410.4321/S2340-989420170003000022340-9894ABSTRACT: The anticoagulant effect and cytotoxicity of a high molecular weight polysaccharide fraction (1000RS) obtained from the tunic of the ascidia Microcosmus exasperatus were evaluated. Methods: Anticoagulant properties of 1000RS was evaluated by activated Partial Thromboplastin Time (aPTT), Thrombin Time (TT), Prothrombin Time (PT), anti-factor Xa and lupic anticoagulant (dRVVT) assays. Cytotoxicity was tested on murine hematopoietic cells using MTT assay. Results: This galactose rich fraction showed to be a potential anticoagulant due to its inhibitory effect on the intrinsic coagulation pathway. At the same time, anticoagulant doses of this fraction have no effect on cellular viability, which means that it can be used as a therapeutic agent. Conclusion: In vitro anticoagulant effect of 1000RS occurs at innocuous doses, however, it still need to be tested using in vivo models and its cytotoxicity evaluated in normal human cell lines.RESUMEN: El efecto anticoagulante y la citotoxicidad de una fracción de polisacáridos de alto peso molecular (1000RS), obtenida de la túnica de la ascidia Microcosmus exasperatus, fueron evaluados. Métodos: La actividad anticoagulante de 1000RS fue evaluada mediante los ensayos de tiempo de tromboplastina parcial activado (TTPa), tiempo de trombina (TT), tiempo de protrombina (TP), anti factor Xa y anticoagulante lúpico (dRVVT). La citotoxicidad sobre las células hematopoyéticas murinas fue evaluada usando el método del MTT. Resultados: Esta fracción rica en galactosa mostró ser un anticoagulante potencial debido a su efecto inhibidor de la vía intrínseca de la coagulación. Así mismo, las dosis anticoagulantes de esta fracción no afectan la viabilidad celular, lo cual ratifica su potencial como agente terapéutico. Conclusión: El efecto anticoagulante in vitro de 1000RS ocurre a dosis inocuas, sin embargo, éste debe ser evaluado en modelos in vivo, así como su citotoxicidad sobre células humanas normales.application/pdfengFacultad de Farmacia, Universidad de GranadaProductos Naturales MarinosEspañainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_2df8fbb1https://purl.org/redcol/resource_type/ARTArtículo de investigaciónhttp://purl.org/coar/version/c_970fb48d4fbd8a85Atribución-NoComercial-CompartirIgual 2.5 Colombia (CC BY-NC-SA 2.5 CO)info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/co/http://purl.org/coar/access_right/c_abf2https://creativecommons.org/licenses/by-nc-sa/4.0/Anticoagulant properties of a high molecular weight polysaccharide fraction (1000RS) of the ascidian Microcosmus exasperatusPropiedades anticoagulantes de una fracción polisacárida de alto peso molecular (1000RS) del ascidian Microcosmus exasperatusAnticoagulantesAnticoagulantsPolisacáridosPolysaccharidesAscidiaSea squirtsMicrocosmos exasperatushttp://aims.fao.org/aos/agrovoc/c_6900Ars Pharmaceutica95101583CC-LICENSElicense_urllicense_urlArtículo de investigacióntext/plain; charset=utf-849https://bibliotecadigital.udea.edu.co/bitstream/10495/13094/2/license_url4afdbb8c545fd630ea7db775da747b2fMD52license_textlicense_texttext/html; charset=utf-80https://bibliotecadigital.udea.edu.co/bitstream/10495/13094/3/license_textd41d8cd98f00b204e9800998ecf8427eMD53license_rdflicense_rdfapplication/rdf+xml; charset=utf-80https://bibliotecadigital.udea.edu.co/bitstream/10495/13094/4/license_rdfd41d8cd98f00b204e9800998ecf8427eMD54LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://bibliotecadigital.udea.edu.co/bitstream/10495/13094/5/license.txt8a4605be74aa9ea9d79846c1fba20a33MD55ORIGINALMartinezAlejandro_2017_AnticoagulantPropertiesMolecular.pdfMartinezAlejandro_2017_AnticoagulantPropertiesMolecular.pdfArtículo de revistaapplication/pdf316951https://bibliotecadigital.udea.edu.co/bitstream/10495/13094/1/MartinezAlejandro_2017_AnticoagulantPropertiesMolecular.pdf06618eef0c18f307747c3db1917bd096MD5110495/13094oai:bibliotecadigital.udea.edu.co:10495/130942023-09-11 12:42:51.08Repositorio Institucional Universidad de Antioquiaandres.perez@udea.edu.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 |