Identification of Levels of Serum Amyloid A and Apolipoprotein A1 in Serum Proteomic Analysis of Neuropsychiatric Systemic Lupus Erythematosus Patients

ABSTRACT: Neuropsychiatric Systemic Lupus Erythematosus (NPSLE) has multiple pathogenic mechanisms that cause diverse manifestations and whose diagnosis is challenging because of the absence of appropriate diagnostic tests. In the present study the application of proteomics using two-dimensional electrophoresis (2D) and mass spectrometry (MS) allowed the comparison of the protein profile of the serum low and high abundance protein fractions of NPSLE patients (NPSLE group) and SLE without neuropsychiatric syndromes (SLE group), Neuropsychiatric syndromes not associated with SLE (NPnoSLE groups), and healthy controls (CTRL group). The gels obtained were digitalized and analyzed with the PDQuest software. The statistical analysis of the spots was performed using the nonparametric Kruskal Wallis and Dunn's multiple comparison tests. Two spots showed significant differences and were identified by MS. Spot 4009 was significantly lower in NPSLE with regard to NPnoSLE (p= 0,004) and was identified as apolipoprotein A1 (APOA1) (score 809-1132). Spot 8001 was significantly higher in NPSLE regarding CTRL and NPnoSLE (p= 0,01 y 0,03, respectively) and was identified as serum amyloid A (SAA) (score 725-2488). The proinflammatory high density lipoproteins (HDL) have been described in SLE. In this HDL the decrease of APOA1 is followed by an increase in SAA. This altered level of both proteins may be related to the inflammatory state that is characteristic of an autoimmune disease like SLE, but this is not specific for NPSLE.

• Autores: Duarte Delgado, Nancy Paola; Lujan Chavarria, Tania Paola; Arbeláez Cortés, Álvaro; García Valencia, Jenny; Zapata, Adriana; Rojas López, Mauricio; Restrepo Escobar, Mauricio; Vásquez Duque, Gloria María; Ortiz Reyes, Blanca Lucía
• Tipo de recurso: Article of investigation
• Fecha de publicación: 2018
• Institución: Universidad de Antioquia
• Repositorio: Repositorio UdeA
• Idioma: eng
• OAI Identifier: oai:bibliotecadigital.udea.edu.co:10495/26118
• Acceso en línea: http://hdl.handle.net/10495/26118
• Palabra clave: Lupus Eritematoso Sistémico; Lupus Erythematosus, Systemic; Proteína Amiloide A Sérica; Serum Amyloid A Protein; Apolipoproteína A-I; Apolipoprotein A-I; Vasculitis por Lupus del Sistema Nervioso Central; Lupus Vasculitis, Central Nervous System
• Rights: openAccess
• License: http://creativecommons.org/licenses/by/2.5/co/