Recognition of extracellular matrix proteins by Paracoccidioides brasiliensis yeast cells

ABSTRACT: The adhesion of microorganism to host cells or extracellular matrix (ECM) proteins is the first step in the establishment of an infectious process. Interaction between Paracoccidioides brasiliensis yeast cells and ECM proteins has been previously noted. In vivo, in the chronic phase of exp...

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Autores:
González Marín, Ángel Augusto
Gómez Giraldo, Beatriz Lucía
Restrepo Moreno, Ángela
Hamilton, Andrew John
Cano Restrepo, Luz Elena
Tipo de recurso:
Article of investigation
Fecha de publicación:
2005
Institución:
Universidad de Antioquia
Repositorio:
Repositorio UdeA
Idioma:
eng
OAI Identifier:
oai:bibliotecadigital.udea.edu.co:10495/24131
Acceso en línea:
http://hdl.handle.net/10495/24131
Palabra clave:
Fibrinogen
Fibrinógeno
Fibronectin
Fibronectinas
Laminin
Laminina
Anticuerpos Antifúngicos
Antibodies, Fungal
Paracoccidioides
Microscopía Fluorescente
Microscopy, Fluorescence
Rights
openAccess
License
http://creativecommons.org/licenses/by-nc/2.5/co/
id UDEA2_7c03f4a4c963fe07fb4a1c0cbe6f9404
oai_identifier_str oai:bibliotecadigital.udea.edu.co:10495/24131
network_acronym_str UDEA2
network_name_str Repositorio UdeA
repository_id_str
dc.title.spa.fl_str_mv Recognition of extracellular matrix proteins by Paracoccidioides brasiliensis yeast cells
title Recognition of extracellular matrix proteins by Paracoccidioides brasiliensis yeast cells
spellingShingle Recognition of extracellular matrix proteins by Paracoccidioides brasiliensis yeast cells
Fibrinogen
Fibrinógeno
Fibronectin
Fibronectinas
Laminin
Laminina
Anticuerpos Antifúngicos
Antibodies, Fungal
Paracoccidioides
Microscopía Fluorescente
Microscopy, Fluorescence
title_short Recognition of extracellular matrix proteins by Paracoccidioides brasiliensis yeast cells
title_full Recognition of extracellular matrix proteins by Paracoccidioides brasiliensis yeast cells
title_fullStr Recognition of extracellular matrix proteins by Paracoccidioides brasiliensis yeast cells
title_full_unstemmed Recognition of extracellular matrix proteins by Paracoccidioides brasiliensis yeast cells
title_sort Recognition of extracellular matrix proteins by Paracoccidioides brasiliensis yeast cells
dc.creator.fl_str_mv González Marín, Ángel Augusto
Gómez Giraldo, Beatriz Lucía
Restrepo Moreno, Ángela
Hamilton, Andrew John
Cano Restrepo, Luz Elena
dc.contributor.author.none.fl_str_mv González Marín, Ángel Augusto
Gómez Giraldo, Beatriz Lucía
Restrepo Moreno, Ángela
Hamilton, Andrew John
Cano Restrepo, Luz Elena
dc.subject.decs.none.fl_str_mv Fibrinogen
Fibrinógeno
Fibronectin
Fibronectinas
Laminin
Laminina
Anticuerpos Antifúngicos
Antibodies, Fungal
Paracoccidioides
Microscopía Fluorescente
Microscopy, Fluorescence
topic Fibrinogen
Fibrinógeno
Fibronectin
Fibronectinas
Laminin
Laminina
Anticuerpos Antifúngicos
Antibodies, Fungal
Paracoccidioides
Microscopía Fluorescente
Microscopy, Fluorescence
description ABSTRACT: The adhesion of microorganism to host cells or extracellular matrix (ECM) proteins is the first step in the establishment of an infectious process. Interaction between Paracoccidioides brasiliensis yeast cells and ECM proteins has been previously noted. In vivo, in the chronic phase of experimental paracoccidioidomycosis (PCM), laminin and fibronectin have been detected on the surface of yeast cells located inside granulomatous lesions. The aim of the present study was to examine the ability of P. brasiliensis yeast cells to interact with extracellular matrix proteins (laminin, fibrinogen and fibronectin) and to establish which molecules were involved in this interaction. Immunofluorescence microscopy and flow cytometry demonstrated that all three ECM proteins tested were able to bind to the surface of P. brasiliensis yeast cells. Treatment with trypsin, chymotrypsin, chitinase, proteinase K or different sugars resulted in no change in laminin binding. In addition, ligand affinity assays were performed using different yeast extracts (total homogenates, b-mercaptoethanol, SDS extracts). These assays demonstrated the presence of 19 and 32-kDa proteins in the cell wall with the ability to bind to laminin, fibrinogen and fibronectin. This interaction could be important in mediating attachment of the fungus to host tissues and may consequently play a role in the pathogenesis of PCM.
publishDate 2005
dc.date.issued.none.fl_str_mv 2005
dc.date.accessioned.none.fl_str_mv 2021-11-15T23:00:29Z
dc.date.available.none.fl_str_mv 2021-11-15T23:00:29Z
dc.type.spa.fl_str_mv info:eu-repo/semantics/article
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dc.type.local.spa.fl_str_mv Artículo de investigación
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dc.identifier.citation.spa.fl_str_mv González A, Gómez BL, Restrepo A, Hamilton AJ, Cano LE. Recognition of extracellular matrix proteins by Paracoccidioides brasiliensis yeast cells. Med Mycol. 2005 Nov;43(7):637-45. doi: 10.1080/13693780500064599.
dc.identifier.issn.none.fl_str_mv 1369-3786
dc.identifier.uri.none.fl_str_mv http://hdl.handle.net/10495/24131
dc.identifier.doi.none.fl_str_mv 10.1080/13693780500064599
dc.identifier.eissn.none.fl_str_mv 1460-2709
identifier_str_mv González A, Gómez BL, Restrepo A, Hamilton AJ, Cano LE. Recognition of extracellular matrix proteins by Paracoccidioides brasiliensis yeast cells. Med Mycol. 2005 Nov;43(7):637-45. doi: 10.1080/13693780500064599.
1369-3786
10.1080/13693780500064599
1460-2709
url http://hdl.handle.net/10495/24131
dc.language.iso.spa.fl_str_mv eng
language eng
dc.relation.ispartofjournalabbrev.spa.fl_str_mv Med. Mycol.
dc.rights.spa.fl_str_mv info:eu-repo/semantics/openAccess
dc.rights.uri.*.fl_str_mv http://creativecommons.org/licenses/by-nc/2.5/co/
dc.rights.accessrights.spa.fl_str_mv http://purl.org/coar/access_right/c_abf2
dc.rights.creativecommons.spa.fl_str_mv https://creativecommons.org/licenses/by-nc/4.0/
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc/2.5/co/
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dc.format.extent.spa.fl_str_mv 9
dc.format.mimetype.spa.fl_str_mv application/pdf
dc.publisher.spa.fl_str_mv Oxford University Press
dc.publisher.group.spa.fl_str_mv Micología Médica y Experimental
Microbiología Molecular
dc.publisher.place.spa.fl_str_mv Oxford, Inglaterra
institution Universidad de Antioquia
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spelling González Marín, Ángel AugustoGómez Giraldo, Beatriz LucíaRestrepo Moreno, ÁngelaHamilton, Andrew JohnCano Restrepo, Luz Elena2021-11-15T23:00:29Z2021-11-15T23:00:29Z2005González A, Gómez BL, Restrepo A, Hamilton AJ, Cano LE. Recognition of extracellular matrix proteins by Paracoccidioides brasiliensis yeast cells. Med Mycol. 2005 Nov;43(7):637-45. doi: 10.1080/13693780500064599.1369-3786http://hdl.handle.net/10495/2413110.1080/136937805000645991460-2709ABSTRACT: The adhesion of microorganism to host cells or extracellular matrix (ECM) proteins is the first step in the establishment of an infectious process. Interaction between Paracoccidioides brasiliensis yeast cells and ECM proteins has been previously noted. In vivo, in the chronic phase of experimental paracoccidioidomycosis (PCM), laminin and fibronectin have been detected on the surface of yeast cells located inside granulomatous lesions. The aim of the present study was to examine the ability of P. brasiliensis yeast cells to interact with extracellular matrix proteins (laminin, fibrinogen and fibronectin) and to establish which molecules were involved in this interaction. Immunofluorescence microscopy and flow cytometry demonstrated that all three ECM proteins tested were able to bind to the surface of P. brasiliensis yeast cells. Treatment with trypsin, chymotrypsin, chitinase, proteinase K or different sugars resulted in no change in laminin binding. In addition, ligand affinity assays were performed using different yeast extracts (total homogenates, b-mercaptoethanol, SDS extracts). These assays demonstrated the presence of 19 and 32-kDa proteins in the cell wall with the ability to bind to laminin, fibrinogen and fibronectin. This interaction could be important in mediating attachment of the fungus to host tissues and may consequently play a role in the pathogenesis of PCM.COL0013709COL00137469application/pdfengOxford University PressMicología Médica y ExperimentalMicrobiología MolecularOxford, Inglaterrainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_2df8fbb1https://purl.org/redcol/resource_type/ARTArtículo de investigaciónhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc/2.5/co/http://purl.org/coar/access_right/c_abf2https://creativecommons.org/licenses/by-nc/4.0/Recognition of extracellular matrix proteins by Paracoccidioides brasiliensis yeast cellsFibrinogenFibrinógenoFibronectinFibronectinasLamininLamininaAnticuerpos AntifúngicosAntibodies, FungalParacoccidioidesMicroscopía FluorescenteMicroscopy, FluorescenceMed. Mycol.Medical Mycology637645437ORIGINALRestrepoAngela_2005_RecognitionMatrix.pdfRestrepoAngela_2005_RecognitionMatrix.pdfArtículo de investigaciónapplication/pdf348016http://bibliotecadigital.udea.edu.co/bitstream/10495/24131/1/RestrepoAngela_2005_RecognitionMatrix.pdff7fbd5fe8b302e33d27a76f1bed30eaeMD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748http://bibliotecadigital.udea.edu.co/bitstream/10495/24131/3/license.txt8a4605be74aa9ea9d79846c1fba20a33MD53CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8933http://bibliotecadigital.udea.edu.co/bitstream/10495/24131/2/license_rdfc0c92b0ffc8b7d22d9cf56754a416a76MD5210495/24131oai:bibliotecadigital.udea.edu.co:10495/241312022-04-22 10:22:10.646Repositorio Institucional Universidad de Antioquiaandres.perez@udea.edu.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