Recognition of extracellular matrix proteins by Paracoccidioides brasiliensis yeast cells
ABSTRACT: The adhesion of microorganism to host cells or extracellular matrix (ECM) proteins is the first step in the establishment of an infectious process. Interaction between Paracoccidioides brasiliensis yeast cells and ECM proteins has been previously noted. In vivo, in the chronic phase of exp...
- Autores:
-
González Marín, Ángel Augusto
Gómez Giraldo, Beatriz Lucía
Restrepo Moreno, Ángela
Hamilton, Andrew John
Cano Restrepo, Luz Elena
- Tipo de recurso:
- Article of investigation
- Fecha de publicación:
- 2005
- Institución:
- Universidad de Antioquia
- Repositorio:
- Repositorio UdeA
- Idioma:
- eng
- OAI Identifier:
- oai:bibliotecadigital.udea.edu.co:10495/24131
- Acceso en línea:
- http://hdl.handle.net/10495/24131
- Palabra clave:
- Fibrinogen
Fibrinógeno
Fibronectin
Fibronectinas
Laminin
Laminina
Anticuerpos Antifúngicos
Antibodies, Fungal
Paracoccidioides
Microscopía Fluorescente
Microscopy, Fluorescence
- Rights
- openAccess
- License
- http://creativecommons.org/licenses/by-nc/2.5/co/
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oai:bibliotecadigital.udea.edu.co:10495/24131 |
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UDEA2 |
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Repositorio UdeA |
repository_id_str |
|
dc.title.spa.fl_str_mv |
Recognition of extracellular matrix proteins by Paracoccidioides brasiliensis yeast cells |
title |
Recognition of extracellular matrix proteins by Paracoccidioides brasiliensis yeast cells |
spellingShingle |
Recognition of extracellular matrix proteins by Paracoccidioides brasiliensis yeast cells Fibrinogen Fibrinógeno Fibronectin Fibronectinas Laminin Laminina Anticuerpos Antifúngicos Antibodies, Fungal Paracoccidioides Microscopía Fluorescente Microscopy, Fluorescence |
title_short |
Recognition of extracellular matrix proteins by Paracoccidioides brasiliensis yeast cells |
title_full |
Recognition of extracellular matrix proteins by Paracoccidioides brasiliensis yeast cells |
title_fullStr |
Recognition of extracellular matrix proteins by Paracoccidioides brasiliensis yeast cells |
title_full_unstemmed |
Recognition of extracellular matrix proteins by Paracoccidioides brasiliensis yeast cells |
title_sort |
Recognition of extracellular matrix proteins by Paracoccidioides brasiliensis yeast cells |
dc.creator.fl_str_mv |
González Marín, Ángel Augusto Gómez Giraldo, Beatriz Lucía Restrepo Moreno, Ángela Hamilton, Andrew John Cano Restrepo, Luz Elena |
dc.contributor.author.none.fl_str_mv |
González Marín, Ángel Augusto Gómez Giraldo, Beatriz Lucía Restrepo Moreno, Ángela Hamilton, Andrew John Cano Restrepo, Luz Elena |
dc.subject.decs.none.fl_str_mv |
Fibrinogen Fibrinógeno Fibronectin Fibronectinas Laminin Laminina Anticuerpos Antifúngicos Antibodies, Fungal Paracoccidioides Microscopía Fluorescente Microscopy, Fluorescence |
topic |
Fibrinogen Fibrinógeno Fibronectin Fibronectinas Laminin Laminina Anticuerpos Antifúngicos Antibodies, Fungal Paracoccidioides Microscopía Fluorescente Microscopy, Fluorescence |
description |
ABSTRACT: The adhesion of microorganism to host cells or extracellular matrix (ECM) proteins is the first step in the establishment of an infectious process. Interaction between Paracoccidioides brasiliensis yeast cells and ECM proteins has been previously noted. In vivo, in the chronic phase of experimental paracoccidioidomycosis (PCM), laminin and fibronectin have been detected on the surface of yeast cells located inside granulomatous lesions. The aim of the present study was to examine the ability of P. brasiliensis yeast cells to interact with extracellular matrix proteins (laminin, fibrinogen and fibronectin) and to establish which molecules were involved in this interaction. Immunofluorescence microscopy and flow cytometry demonstrated that all three ECM proteins tested were able to bind to the surface of P. brasiliensis yeast cells. Treatment with trypsin, chymotrypsin, chitinase, proteinase K or different sugars resulted in no change in laminin binding. In addition, ligand affinity assays were performed using different yeast extracts (total homogenates, b-mercaptoethanol, SDS extracts). These assays demonstrated the presence of 19 and 32-kDa proteins in the cell wall with the ability to bind to laminin, fibrinogen and fibronectin. This interaction could be important in mediating attachment of the fungus to host tissues and may consequently play a role in the pathogenesis of PCM. |
publishDate |
2005 |
dc.date.issued.none.fl_str_mv |
2005 |
dc.date.accessioned.none.fl_str_mv |
2021-11-15T23:00:29Z |
dc.date.available.none.fl_str_mv |
2021-11-15T23:00:29Z |
dc.type.spa.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.coarversion.fl_str_mv |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
dc.type.hasversion.spa.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.coar.spa.fl_str_mv |
http://purl.org/coar/resource_type/c_2df8fbb1 |
dc.type.redcol.spa.fl_str_mv |
https://purl.org/redcol/resource_type/ART |
dc.type.local.spa.fl_str_mv |
Artículo de investigación |
format |
http://purl.org/coar/resource_type/c_2df8fbb1 |
status_str |
publishedVersion |
dc.identifier.citation.spa.fl_str_mv |
González A, Gómez BL, Restrepo A, Hamilton AJ, Cano LE. Recognition of extracellular matrix proteins by Paracoccidioides brasiliensis yeast cells. Med Mycol. 2005 Nov;43(7):637-45. doi: 10.1080/13693780500064599. |
dc.identifier.issn.none.fl_str_mv |
1369-3786 |
dc.identifier.uri.none.fl_str_mv |
http://hdl.handle.net/10495/24131 |
dc.identifier.doi.none.fl_str_mv |
10.1080/13693780500064599 |
dc.identifier.eissn.none.fl_str_mv |
1460-2709 |
identifier_str_mv |
González A, Gómez BL, Restrepo A, Hamilton AJ, Cano LE. Recognition of extracellular matrix proteins by Paracoccidioides brasiliensis yeast cells. Med Mycol. 2005 Nov;43(7):637-45. doi: 10.1080/13693780500064599. 1369-3786 10.1080/13693780500064599 1460-2709 |
url |
http://hdl.handle.net/10495/24131 |
dc.language.iso.spa.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartofjournalabbrev.spa.fl_str_mv |
Med. Mycol. |
dc.rights.spa.fl_str_mv |
info:eu-repo/semantics/openAccess |
dc.rights.uri.*.fl_str_mv |
http://creativecommons.org/licenses/by-nc/2.5/co/ |
dc.rights.accessrights.spa.fl_str_mv |
http://purl.org/coar/access_right/c_abf2 |
dc.rights.creativecommons.spa.fl_str_mv |
https://creativecommons.org/licenses/by-nc/4.0/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by-nc/2.5/co/ http://purl.org/coar/access_right/c_abf2 https://creativecommons.org/licenses/by-nc/4.0/ |
dc.format.extent.spa.fl_str_mv |
9 |
dc.format.mimetype.spa.fl_str_mv |
application/pdf |
dc.publisher.spa.fl_str_mv |
Oxford University Press |
dc.publisher.group.spa.fl_str_mv |
Micología Médica y Experimental Microbiología Molecular |
dc.publisher.place.spa.fl_str_mv |
Oxford, Inglaterra |
institution |
Universidad de Antioquia |
bitstream.url.fl_str_mv |
http://bibliotecadigital.udea.edu.co/bitstream/10495/24131/1/RestrepoAngela_2005_RecognitionMatrix.pdf http://bibliotecadigital.udea.edu.co/bitstream/10495/24131/3/license.txt http://bibliotecadigital.udea.edu.co/bitstream/10495/24131/2/license_rdf |
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bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 MD5 |
repository.name.fl_str_mv |
Repositorio Institucional Universidad de Antioquia |
repository.mail.fl_str_mv |
andres.perez@udea.edu.co |
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1812173110273638400 |
spelling |
González Marín, Ángel AugustoGómez Giraldo, Beatriz LucíaRestrepo Moreno, ÁngelaHamilton, Andrew JohnCano Restrepo, Luz Elena2021-11-15T23:00:29Z2021-11-15T23:00:29Z2005González A, Gómez BL, Restrepo A, Hamilton AJ, Cano LE. Recognition of extracellular matrix proteins by Paracoccidioides brasiliensis yeast cells. Med Mycol. 2005 Nov;43(7):637-45. doi: 10.1080/13693780500064599.1369-3786http://hdl.handle.net/10495/2413110.1080/136937805000645991460-2709ABSTRACT: The adhesion of microorganism to host cells or extracellular matrix (ECM) proteins is the first step in the establishment of an infectious process. Interaction between Paracoccidioides brasiliensis yeast cells and ECM proteins has been previously noted. In vivo, in the chronic phase of experimental paracoccidioidomycosis (PCM), laminin and fibronectin have been detected on the surface of yeast cells located inside granulomatous lesions. The aim of the present study was to examine the ability of P. brasiliensis yeast cells to interact with extracellular matrix proteins (laminin, fibrinogen and fibronectin) and to establish which molecules were involved in this interaction. Immunofluorescence microscopy and flow cytometry demonstrated that all three ECM proteins tested were able to bind to the surface of P. brasiliensis yeast cells. Treatment with trypsin, chymotrypsin, chitinase, proteinase K or different sugars resulted in no change in laminin binding. In addition, ligand affinity assays were performed using different yeast extracts (total homogenates, b-mercaptoethanol, SDS extracts). These assays demonstrated the presence of 19 and 32-kDa proteins in the cell wall with the ability to bind to laminin, fibrinogen and fibronectin. This interaction could be important in mediating attachment of the fungus to host tissues and may consequently play a role in the pathogenesis of PCM.COL0013709COL00137469application/pdfengOxford University PressMicología Médica y ExperimentalMicrobiología MolecularOxford, Inglaterrainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_2df8fbb1https://purl.org/redcol/resource_type/ARTArtículo de investigaciónhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc/2.5/co/http://purl.org/coar/access_right/c_abf2https://creativecommons.org/licenses/by-nc/4.0/Recognition of extracellular matrix proteins by Paracoccidioides brasiliensis yeast cellsFibrinogenFibrinógenoFibronectinFibronectinasLamininLamininaAnticuerpos AntifúngicosAntibodies, FungalParacoccidioidesMicroscopía FluorescenteMicroscopy, FluorescenceMed. Mycol.Medical Mycology637645437ORIGINALRestrepoAngela_2005_RecognitionMatrix.pdfRestrepoAngela_2005_RecognitionMatrix.pdfArtículo de investigaciónapplication/pdf348016http://bibliotecadigital.udea.edu.co/bitstream/10495/24131/1/RestrepoAngela_2005_RecognitionMatrix.pdff7fbd5fe8b302e33d27a76f1bed30eaeMD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748http://bibliotecadigital.udea.edu.co/bitstream/10495/24131/3/license.txt8a4605be74aa9ea9d79846c1fba20a33MD53CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8933http://bibliotecadigital.udea.edu.co/bitstream/10495/24131/2/license_rdfc0c92b0ffc8b7d22d9cf56754a416a76MD5210495/24131oai:bibliotecadigital.udea.edu.co:10495/241312022-04-22 10:22:10.646Repositorio Institucional Universidad de Antioquiaandres.perez@udea.edu.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 |