Evaluation of a Cryptococcal antigen Lateral Flow Assay in serum and cerebrospinal fluid for rapid diagnosis of cryptococcosis in Colombia
ABSTRACT : A Lateral Flow Assay to detect cryptococcal antigen (CrAg® LFA) in serum and cerebrospinal fluid for the rapid diagnosis of cryptococcosis was evaluated. A retrospective validation was performed. Sensitivity and specificity of the CrAg® LFA was 100%. High concordance (kappa index=1.0) bet...
- Autores:
-
Cáceres Contreras, Diego Hernando
Zuluaga, Alejandra
Tabares Bohórquez, Ángela María
Chiller, Tom
González Marín, Ángel Augusto
Gómez Giraldo, Beatriz Lucía
- Tipo de recurso:
- Article of investigation
- Fecha de publicación:
- 2017
- Institución:
- Universidad de Antioquia
- Repositorio:
- Repositorio UdeA
- Idioma:
- eng
- OAI Identifier:
- oai:bibliotecadigital.udea.edu.co:10495/26496
- Acceso en línea:
- http://hdl.handle.net/10495/26496
- Palabra clave:
- Criptococosis
Cryptococcosis
Diagnóstico
Diagnosis
Antígenos
Antigens
Sistemas de Atención de Punto
Point-of-Care Systems
Inmunoensayo
Immunoassay
- Rights
- openAccess
- License
- https://creativecommons.org/licenses/by-nc-sa/4.0/
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| dc.title.spa.fl_str_mv |
Evaluation of a Cryptococcal antigen Lateral Flow Assay in serum and cerebrospinal fluid for rapid diagnosis of cryptococcosis in Colombia |
| title |
Evaluation of a Cryptococcal antigen Lateral Flow Assay in serum and cerebrospinal fluid for rapid diagnosis of cryptococcosis in Colombia |
| spellingShingle |
Evaluation of a Cryptococcal antigen Lateral Flow Assay in serum and cerebrospinal fluid for rapid diagnosis of cryptococcosis in Colombia Criptococosis Cryptococcosis Diagnóstico Diagnosis Antígenos Antigens Sistemas de Atención de Punto Point-of-Care Systems Inmunoensayo Immunoassay |
| title_short |
Evaluation of a Cryptococcal antigen Lateral Flow Assay in serum and cerebrospinal fluid for rapid diagnosis of cryptococcosis in Colombia |
| title_full |
Evaluation of a Cryptococcal antigen Lateral Flow Assay in serum and cerebrospinal fluid for rapid diagnosis of cryptococcosis in Colombia |
| title_fullStr |
Evaluation of a Cryptococcal antigen Lateral Flow Assay in serum and cerebrospinal fluid for rapid diagnosis of cryptococcosis in Colombia |
| title_full_unstemmed |
Evaluation of a Cryptococcal antigen Lateral Flow Assay in serum and cerebrospinal fluid for rapid diagnosis of cryptococcosis in Colombia |
| title_sort |
Evaluation of a Cryptococcal antigen Lateral Flow Assay in serum and cerebrospinal fluid for rapid diagnosis of cryptococcosis in Colombia |
| dc.creator.fl_str_mv |
Cáceres Contreras, Diego Hernando Zuluaga, Alejandra Tabares Bohórquez, Ángela María Chiller, Tom González Marín, Ángel Augusto Gómez Giraldo, Beatriz Lucía |
| dc.contributor.author.none.fl_str_mv |
Cáceres Contreras, Diego Hernando Zuluaga, Alejandra Tabares Bohórquez, Ángela María Chiller, Tom González Marín, Ángel Augusto Gómez Giraldo, Beatriz Lucía |
| dc.contributor.researchgroup.spa.fl_str_mv |
Grupo de Investigación en Microbiología Básica y Aplicada-Microba |
| dc.subject.decs.none.fl_str_mv |
Criptococosis Cryptococcosis Diagnóstico Diagnosis Antígenos Antigens Sistemas de Atención de Punto Point-of-Care Systems Inmunoensayo Immunoassay |
| topic |
Criptococosis Cryptococcosis Diagnóstico Diagnosis Antígenos Antigens Sistemas de Atención de Punto Point-of-Care Systems Inmunoensayo Immunoassay |
| description |
ABSTRACT : A Lateral Flow Assay to detect cryptococcal antigen (CrAg® LFA) in serum and cerebrospinal fluid for the rapid diagnosis of cryptococcosis was evaluated. A retrospective validation was performed. Sensitivity and specificity of the CrAg® LFA was 100%. High concordance (kappa index=1.0) between Cryptococcal Antigen Latex Agglutination System (CALAS®) and CrAg® LFA was observed. CrAg® LFA showed higher analytical sensitivity for detecting low concentrations of cryptococcal antigen. |
| publishDate |
2017 |
| dc.date.issued.none.fl_str_mv |
2017 |
| dc.date.accessioned.none.fl_str_mv |
2022-03-10T15:23:02Z |
| dc.date.available.none.fl_str_mv |
2022-03-10T15:23:02Z |
| dc.type.spa.fl_str_mv |
Artículo de investigación |
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http://purl.org/coar/resource_type/c_2df8fbb1 |
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https://purl.org/redcol/resource_type/ART |
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http://purl.org/coar/version/c_970fb48d4fbd8a85 |
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info:eu-repo/semantics/article |
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info:eu-repo/semantics/publishedVersion |
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http://purl.org/coar/resource_type/c_2df8fbb1 |
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0036-4665 |
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http://hdl.handle.net/10495/26496 |
| dc.identifier.doi.none.fl_str_mv |
10.1590/S1678-9946201759076 |
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1678-9946 |
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0036-4665 10.1590/S1678-9946201759076 1678-9946 |
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http://hdl.handle.net/10495/26496 |
| dc.language.iso.spa.fl_str_mv |
eng |
| language |
eng |
| dc.relation.ispartofjournalabbrev.spa.fl_str_mv |
Rev. Inst. Med. Trop. São Paulo. |
| dc.relation.citationendpage.spa.fl_str_mv |
3 |
| dc.relation.citationissue.spa.fl_str_mv |
76 |
| dc.relation.citationstartpage.spa.fl_str_mv |
1 |
| dc.relation.citationvolume.spa.fl_str_mv |
59 |
| dc.relation.ispartofjournal.spa.fl_str_mv |
Revista do Instituto de Medicina Tropical de Sao Paulo |
| dc.rights.uri.spa.fl_str_mv |
https://creativecommons.org/licenses/by-nc-sa/4.0/ |
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http://creativecommons.org/licenses/by-nc-sa/2.5/co/ |
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application/pdf |
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Universidade de São Paulo, Faculdade de Medicina |
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Sao Paulo, Brasil |
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Universidad de Antioquia |
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Cáceres Contreras, Diego HernandoZuluaga, AlejandraTabares Bohórquez, Ángela MaríaChiller, TomGonzález Marín, Ángel AugustoGómez Giraldo, Beatriz LucíaGrupo de Investigación en Microbiología Básica y Aplicada-Microba2022-03-10T15:23:02Z2022-03-10T15:23:02Z20170036-4665http://hdl.handle.net/10495/2649610.1590/S1678-99462017590761678-9946ABSTRACT : A Lateral Flow Assay to detect cryptococcal antigen (CrAg® LFA) in serum and cerebrospinal fluid for the rapid diagnosis of cryptococcosis was evaluated. A retrospective validation was performed. Sensitivity and specificity of the CrAg® LFA was 100%. High concordance (kappa index=1.0) between Cryptococcal Antigen Latex Agglutination System (CALAS®) and CrAg® LFA was observed. CrAg® LFA showed higher analytical sensitivity for detecting low concentrations of cryptococcal antigen.COL01261313application/pdfengUniversidade de São Paulo, Faculdade de MedicinaSao Paulo, Brasilhttps://creativecommons.org/licenses/by-nc-sa/4.0/http://creativecommons.org/licenses/by-nc-sa/2.5/co/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Evaluation of a Cryptococcal antigen Lateral Flow Assay in serum and cerebrospinal fluid for rapid diagnosis of cryptococcosis in ColombiaArtículo de investigaciónhttp://purl.org/coar/resource_type/c_2df8fbb1https://purl.org/redcol/resource_type/ARThttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionCriptococosisCryptococcosisDiagnósticoDiagnosisAntígenosAntigensSistemas de Atención de PuntoPoint-of-Care SystemsInmunoensayoImmunoassayRev. Inst. Med. Trop. São Paulo.376159Revista do Instituto de Medicina Tropical de Sao PauloPublicationLICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://bibliotecadigital.udea.edu.co/bitstreams/0eca35d3-033f-4e9b-81ac-ee6fea641229/download8a4605be74aa9ea9d79846c1fba20a33MD53falseAnonymousREADORIGINALGonzálezÁngel_2017_EvaluationCryptococcalAntigen.pdfGonzálezÁngel_2017_EvaluationCryptococcalAntigen.pdfArtículo de investigaciónapplication/pdf259640https://bibliotecadigital.udea.edu.co/bitstreams/fbb64cec-f534-471b-8d9d-3d5ae7a610d5/download9dd395a0f182419b2df201144d3bd193MD51trueAnonymousREADCC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-81051https://bibliotecadigital.udea.edu.co/bitstreams/98f0c3f9-0143-43f9-8120-351e908f78ed/downloade2060682c9c70d4d30c83c51448f4eedMD52falseAnonymousREADTEXTGonzálezÁngel_2017_EvaluationCryptococcalAntigen.pdf.txtGonzálezÁngel_2017_EvaluationCryptococcalAntigen.pdf.txtExtracted texttext/plain11754https://bibliotecadigital.udea.edu.co/bitstreams/a5f22f7d-6e80-4f37-8c35-47bcb1111706/download01d42ad15fa81a01a145e524a0322da1MD54falseAnonymousREADTHUMBNAILGonzálezÁngel_2017_EvaluationCryptococcalAntigen.pdf.jpgGonzálezÁngel_2017_EvaluationCryptococcalAntigen.pdf.jpgGenerated Thumbnailimage/jpeg15458https://bibliotecadigital.udea.edu.co/bitstreams/c8d521bd-3de2-4c4b-9cdb-5c8ddf4e9be3/downloadd7836d17a0102194cea488934496961dMD55falseAnonymousREAD10495/26496oai:bibliotecadigital.udea.edu.co:10495/264962025-03-27 00:59:38.014https://creativecommons.org/licenses/by-nc-sa/4.0/open.accesshttps://bibliotecadigital.udea.edu.coRepositorio Institucional de la Universidad de Antioquiaaplicacionbibliotecadigitalbiblioteca@udea.edu.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 |