Acute Oral Toxicity from a Fraction Rich in Phenolic Compounds from the Leaf Extract of Swietenia macrophylla King in a Murine Model

ABSTRACT: The mahogany tree (Swietenia macrophylla King) is widely used in traditional medicine, especially its seeds, which are used in Malaysia, Indonesia and some countries of South America. Recently an active fraction of the leaf ethanolic extract (labelled “Sm13-16,23”), showed promising result...

Full description

Autores:
Henao Duque, Ana María
Rodríguez, Berardo de Jesús
Pereañez Jiménes, Jaime Andres
Lobo Echeverri, Tatiana
Núñez Rangel, Vitelbina
Tipo de recurso:
Article of investigation
Fecha de publicación:
2017
Institución:
Universidad de Antioquia
Repositorio:
Repositorio UdeA
Idioma:
eng
OAI Identifier:
oai:bibliotecadigital.udea.edu.co:10495/20243
Acceso en línea:
http://hdl.handle.net/10495/20243
Palabra clave:
Toxicología
Toxicology
Toxicidad Aguda
Acute Toxicity
Swietenia macrophylla
Extractos de hoja
Leaf extracts
http://aims.fao.org/aos/agrovoc/c_32552
http://aims.fao.org/aos/agrovoc/c_35308
Rights
openAccess
License
http://creativecommons.org/licenses/by-nc-sa/2.5/co/
id UDEA2_6fffa070b3481c01c5ed7167b94a6833
oai_identifier_str oai:bibliotecadigital.udea.edu.co:10495/20243
network_acronym_str UDEA2
network_name_str Repositorio UdeA
repository_id_str
dc.title.spa.fl_str_mv Acute Oral Toxicity from a Fraction Rich in Phenolic Compounds from the Leaf Extract of Swietenia macrophylla King in a Murine Model
dc.title.alternative.spa.fl_str_mv Determinación de la toxicidad aguda oral de una fracción rica en compuestos fenólicos del extracto de hojas de swietenia macrophylla king en un modelo murino
title Acute Oral Toxicity from a Fraction Rich in Phenolic Compounds from the Leaf Extract of Swietenia macrophylla King in a Murine Model
spellingShingle Acute Oral Toxicity from a Fraction Rich in Phenolic Compounds from the Leaf Extract of Swietenia macrophylla King in a Murine Model
Toxicología
Toxicology
Toxicidad Aguda
Acute Toxicity
Swietenia macrophylla
Extractos de hoja
Leaf extracts
http://aims.fao.org/aos/agrovoc/c_32552
http://aims.fao.org/aos/agrovoc/c_35308
title_short Acute Oral Toxicity from a Fraction Rich in Phenolic Compounds from the Leaf Extract of Swietenia macrophylla King in a Murine Model
title_full Acute Oral Toxicity from a Fraction Rich in Phenolic Compounds from the Leaf Extract of Swietenia macrophylla King in a Murine Model
title_fullStr Acute Oral Toxicity from a Fraction Rich in Phenolic Compounds from the Leaf Extract of Swietenia macrophylla King in a Murine Model
title_full_unstemmed Acute Oral Toxicity from a Fraction Rich in Phenolic Compounds from the Leaf Extract of Swietenia macrophylla King in a Murine Model
title_sort Acute Oral Toxicity from a Fraction Rich in Phenolic Compounds from the Leaf Extract of Swietenia macrophylla King in a Murine Model
dc.creator.fl_str_mv Henao Duque, Ana María
Rodríguez, Berardo de Jesús
Pereañez Jiménes, Jaime Andres
Lobo Echeverri, Tatiana
Núñez Rangel, Vitelbina
dc.contributor.author.none.fl_str_mv Henao Duque, Ana María
Rodríguez, Berardo de Jesús
Pereañez Jiménes, Jaime Andres
Lobo Echeverri, Tatiana
Núñez Rangel, Vitelbina
dc.subject.decs.none.fl_str_mv Toxicología
Toxicology
Toxicidad Aguda
Acute Toxicity
topic Toxicología
Toxicology
Toxicidad Aguda
Acute Toxicity
Swietenia macrophylla
Extractos de hoja
Leaf extracts
http://aims.fao.org/aos/agrovoc/c_32552
http://aims.fao.org/aos/agrovoc/c_35308
dc.subject.agrovoc.none.fl_str_mv Swietenia macrophylla
Extractos de hoja
Leaf extracts
dc.subject.agrovocuri.none.fl_str_mv http://aims.fao.org/aos/agrovoc/c_32552
http://aims.fao.org/aos/agrovoc/c_35308
description ABSTRACT: The mahogany tree (Swietenia macrophylla King) is widely used in traditional medicine, especially its seeds, which are used in Malaysia, Indonesia and some countries of South America. Recently an active fraction of the leaf ethanolic extract (labelled “Sm13-16,23”), showed promising results against some toxins of South American pit vipers. Objective: In this study the aim was to evaluate the acute oral toxicity of fraction Sm13-16,23 from the leaf ethanolic extract, using Swiss Webster mice. Materials and Methods: Fraction Sm13-16,23 was administered orally in a single dose to 2000 mg/kg and 300 mg/kg, clinical follow-up for 14 days was performed and then euthanasia, necropsy and histopathology of organs were performed. Results: Overall, there were not deaths recorded during the study period. Further not signs of toxicity in doses of 300mg/Kg were observed, but at doses of 2000 mg/kg, histopathological lesions in the liver, as karyomegaly and the binucleation were observed. Conclusion: Fraction Sm13-16,23 does not produce toxicity lesion at doses of 300mg/kg, indicating that the acute oral toxicity risk is low.
publishDate 2017
dc.date.issued.none.fl_str_mv 2017
dc.date.accessioned.none.fl_str_mv 2021-06-22T19:55:21Z
dc.date.available.none.fl_str_mv 2021-06-22T19:55:21Z
dc.type.spa.fl_str_mv info:eu-repo/semantics/article
dc.type.coarversion.fl_str_mv http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.hasversion.spa.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.coar.spa.fl_str_mv http://purl.org/coar/resource_type/c_2df8fbb1
dc.type.redcol.spa.fl_str_mv https://purl.org/redcol/resource_type/ART
dc.type.local.spa.fl_str_mv Artículo de investigación
format http://purl.org/coar/resource_type/c_2df8fbb1
status_str publishedVersion
dc.identifier.issn.none.fl_str_mv 0121-4004
dc.identifier.uri.none.fl_str_mv http://hdl.handle.net/10495/20243
dc.identifier.doi.none.fl_str_mv 10.17533/udea.vitae.v24n1a03
dc.identifier.eissn.none.fl_str_mv 2145-2660
identifier_str_mv 0121-4004
10.17533/udea.vitae.v24n1a03
2145-2660
url http://hdl.handle.net/10495/20243
dc.language.iso.spa.fl_str_mv eng
language eng
dc.relation.ispartofjournalabbrev.spa.fl_str_mv Vitae
dc.rights.spa.fl_str_mv info:eu-repo/semantics/openAccess
dc.rights.uri.*.fl_str_mv http://creativecommons.org/licenses/by-nc-sa/2.5/co/
dc.rights.accessrights.spa.fl_str_mv http://purl.org/coar/access_right/c_abf2
dc.rights.creativecommons.spa.fl_str_mv https://creativecommons.org/licenses/by-nc-sa/4.0/
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/2.5/co/
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by-nc-sa/4.0/
dc.format.extent.spa.fl_str_mv 7
dc.format.mimetype.spa.fl_str_mv application/pdf
dc.publisher.spa.fl_str_mv Universidad de Antioquia, Facultad de Ciencias Farmacéuticas y Alimentarias
dc.publisher.group.spa.fl_str_mv Grupo de Investigación en Patobiología – QUIRÓN
Toxinología Alternativas Terapéuticas y Alimentarias
dc.publisher.place.spa.fl_str_mv Medellín, Colombia
institution Universidad de Antioquia
bitstream.url.fl_str_mv http://bibliotecadigital.udea.edu.co/bitstream/10495/20243/1/Perea%c3%b1ezJaime_2017_AcuteOralToxicity.pdf
http://bibliotecadigital.udea.edu.co/bitstream/10495/20243/2/license_rdf
http://bibliotecadigital.udea.edu.co/bitstream/10495/20243/3/license.txt
bitstream.checksum.fl_str_mv d3f7d80d114c4732d8c0ffc63ea4fcc3
e2060682c9c70d4d30c83c51448f4eed
8a4605be74aa9ea9d79846c1fba20a33
bitstream.checksumAlgorithm.fl_str_mv MD5
MD5
MD5
repository.name.fl_str_mv Repositorio Institucional Universidad de Antioquia
repository.mail.fl_str_mv andres.perez@udea.edu.co
_version_ 1812173102615887872
spelling Henao Duque, Ana MaríaRodríguez, Berardo de JesúsPereañez Jiménes, Jaime AndresLobo Echeverri, TatianaNúñez Rangel, Vitelbina2021-06-22T19:55:21Z2021-06-22T19:55:21Z20170121-4004http://hdl.handle.net/10495/2024310.17533/udea.vitae.v24n1a032145-2660ABSTRACT: The mahogany tree (Swietenia macrophylla King) is widely used in traditional medicine, especially its seeds, which are used in Malaysia, Indonesia and some countries of South America. Recently an active fraction of the leaf ethanolic extract (labelled “Sm13-16,23”), showed promising results against some toxins of South American pit vipers. Objective: In this study the aim was to evaluate the acute oral toxicity of fraction Sm13-16,23 from the leaf ethanolic extract, using Swiss Webster mice. Materials and Methods: Fraction Sm13-16,23 was administered orally in a single dose to 2000 mg/kg and 300 mg/kg, clinical follow-up for 14 days was performed and then euthanasia, necropsy and histopathology of organs were performed. Results: Overall, there were not deaths recorded during the study period. Further not signs of toxicity in doses of 300mg/Kg were observed, but at doses of 2000 mg/kg, histopathological lesions in the liver, as karyomegaly and the binucleation were observed. Conclusion: Fraction Sm13-16,23 does not produce toxicity lesion at doses of 300mg/kg, indicating that the acute oral toxicity risk is low.RESUMEN: El árbol de la caoba (Swietenia macrophylla King) es utilizado ampliamente en la medicina tradicional, especialmente sus semillas, que son usadas en Malasia, Indonesia y algunos países de Suramérica. Recientemente una fracción activa del extracto etanólico de las hojas (llamada “Sm13-16,23”), demostró resultados promisorios frente algunas toxinas de las víboras suramericanas. Objetivos: En este estudio se evaluó la toxicidad aguda oral de la fracción Sm13-16,23 del extracto etanólico de las hojas de S. macrophylla utilizando ratones Swiss Webster. Métodos: La fracción Sm13-16,23 se administró vía oral como dosis única a 2000 mg/kg y 300 mg/kg, se realizó seguimiento clínico de los animales durante 14 días y finalmente se les aplicó la eutanasia. Se practicó la necropsia de cada animal y se tomaron algunos órganos para su evaluación histopatológica. Resultados: No se registraron muertes durante el tiempo de estudio, ni se evidenciaron signos de toxicidad a dosis de 300mg/kg, sin embargo, a la dosis de 2000 mg/kg, se encontraron lesiones histopatológicas en el hígado; como cariomegalia y binucleación de los hepatocitos. Conclusión: La fracción Sm13-16,23 no produjo lesiones de toxicidad a dosis de 300mg/ kg, lo que indicia que el riesgo de toxicidad aguda oral es bajo.COL0014476COL00929647application/pdfengUniversidad de Antioquia, Facultad de Ciencias Farmacéuticas y AlimentariasGrupo de Investigación en Patobiología – QUIRÓNToxinología Alternativas Terapéuticas y AlimentariasMedellín, Colombiainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_2df8fbb1https://purl.org/redcol/resource_type/ARTArtículo de investigaciónhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/2.5/co/http://purl.org/coar/access_right/c_abf2https://creativecommons.org/licenses/by-nc-sa/4.0/Acute Oral Toxicity from a Fraction Rich in Phenolic Compounds from the Leaf Extract of Swietenia macrophylla King in a Murine ModelDeterminación de la toxicidad aguda oral de una fracción rica en compuestos fenólicos del extracto de hojas de swietenia macrophylla king en un modelo murinoToxicologíaToxicologyToxicidad AgudaAcute ToxicitySwietenia macrophyllaExtractos de hojaLeaf extractshttp://aims.fao.org/aos/agrovoc/c_32552http://aims.fao.org/aos/agrovoc/c_35308VitaeVitae2329241ORIGINALPereañezJaime_2017_AcuteOralToxicity.pdfPereañezJaime_2017_AcuteOralToxicity.pdfArtículo de investigaciónapplication/pdf902322http://bibliotecadigital.udea.edu.co/bitstream/10495/20243/1/Perea%c3%b1ezJaime_2017_AcuteOralToxicity.pdfd3f7d80d114c4732d8c0ffc63ea4fcc3MD51CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-81051http://bibliotecadigital.udea.edu.co/bitstream/10495/20243/2/license_rdfe2060682c9c70d4d30c83c51448f4eedMD52LICENSElicense.txtlicense.txttext/plain; charset=utf-81748http://bibliotecadigital.udea.edu.co/bitstream/10495/20243/3/license.txt8a4605be74aa9ea9d79846c1fba20a33MD5310495/20243oai:bibliotecadigital.udea.edu.co:10495/202432021-06-22 14:55:22.145Repositorio Institucional Universidad de Antioquiaandres.perez@udea.edu.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