Antimicrobial potential of a hydrolyzed protein extract of the microalgae Nannochloropsis sp.
ABSTRACT : Microalgae are photosynthetic microorganisms widely used at the industrial level because of their high content of compounds with bioactive properties such as antimicrobial peptides. These compounds have a wide range of action mechanisms, do not produce the resistance of conventional antib...
- Autores:
-
Verdugo González, Lucía
Acosta Cárdenas, Alejandro
Zapata Zapata, Arley David
Cadena Chamorro, Edith Marleny
Pulido Muñoz, Sergio Andrés
- Tipo de recurso:
- Article of investigation
- Fecha de publicación:
- 2019
- Institución:
- Universidad de Antioquia
- Repositorio:
- Repositorio UdeA
- Idioma:
- eng
- OAI Identifier:
- oai:bibliotecadigital.udea.edu.co:10495/29448
- Acceso en línea:
- http://hdl.handle.net/10495/29448
- Palabra clave:
- Hidrólisis
Hydrolysis
Productos con Acción Antimicrobiana
Products with Antimicrobial Action
microalgal protein
Nannochloropsis sp.
Proteína microalgal
- Rights
- openAccess
- License
- http://creativecommons.org/licenses/by-nc-nd/2.5/co/
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| dc.title.spa.fl_str_mv |
Antimicrobial potential of a hydrolyzed protein extract of the microalgae Nannochloropsis sp. |
| dc.title.alternative.spa.fl_str_mv |
Potencial antimicrobiano de un extracto de proteína hidrolizado de la microalga Nannochloropsis sp. |
| title |
Antimicrobial potential of a hydrolyzed protein extract of the microalgae Nannochloropsis sp. |
| spellingShingle |
Antimicrobial potential of a hydrolyzed protein extract of the microalgae Nannochloropsis sp. Hidrólisis Hydrolysis Productos con Acción Antimicrobiana Products with Antimicrobial Action microalgal protein Nannochloropsis sp. Proteína microalgal |
| title_short |
Antimicrobial potential of a hydrolyzed protein extract of the microalgae Nannochloropsis sp. |
| title_full |
Antimicrobial potential of a hydrolyzed protein extract of the microalgae Nannochloropsis sp. |
| title_fullStr |
Antimicrobial potential of a hydrolyzed protein extract of the microalgae Nannochloropsis sp. |
| title_full_unstemmed |
Antimicrobial potential of a hydrolyzed protein extract of the microalgae Nannochloropsis sp. |
| title_sort |
Antimicrobial potential of a hydrolyzed protein extract of the microalgae Nannochloropsis sp. |
| dc.creator.fl_str_mv |
Verdugo González, Lucía Acosta Cárdenas, Alejandro Zapata Zapata, Arley David Cadena Chamorro, Edith Marleny Pulido Muñoz, Sergio Andrés |
| dc.contributor.author.none.fl_str_mv |
Verdugo González, Lucía Acosta Cárdenas, Alejandro Zapata Zapata, Arley David Cadena Chamorro, Edith Marleny Pulido Muñoz, Sergio Andrés |
| dc.subject.decs.none.fl_str_mv |
Hidrólisis Hydrolysis Productos con Acción Antimicrobiana Products with Antimicrobial Action |
| topic |
Hidrólisis Hydrolysis Productos con Acción Antimicrobiana Products with Antimicrobial Action microalgal protein Nannochloropsis sp. Proteína microalgal |
| dc.subject.proposal.spa.fl_str_mv |
microalgal protein Nannochloropsis sp. Proteína microalgal |
| description |
ABSTRACT : Microalgae are photosynthetic microorganisms widely used at the industrial level because of their high content of compounds with bioactive properties such as antimicrobial peptides. These compounds have a wide range of action mechanisms, do not produce the resistance of conventional antibiotics and could be effective against bacteria, fungi, viruses, parasites, and even tumor cells. In this work, by mechanical cell lysis treatment, a protein extract of dry biomass of Nannochloropsis sp was obtained, subsequently, it was hydrolyzed with an enzyme complex of proteases, papain, and pancreatin, for 6 hours, at 44 ° C and pH 7. Finally, the antimicrobial activity of the fraction of hydrolyzed extract with a size less than 3 kDa was evaluated against Staphylococcus aureus, Escherichia coli, and Candida albicans, obtaining a percentage of growth inhibition of 93.1%, 96.6%, and 14.4% respectively, which represents a peptic extract with bacteriostatic and fungistatic properties. |
| publishDate |
2019 |
| dc.date.issued.none.fl_str_mv |
2019 |
| dc.date.accessioned.none.fl_str_mv |
2022-06-28T23:59:32Z |
| dc.date.available.none.fl_str_mv |
2022-06-28T23:59:32Z |
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info:eu-repo/semantics/article |
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http://purl.org/coar/version/c_970fb48d4fbd8a85 |
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info:eu-repo/semantics/publishedVersion |
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http://purl.org/coar/resource_type/c_2df8fbb1 |
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https://purl.org/redcol/resource_type/ART |
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Artículo de investigación |
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http://purl.org/coar/resource_type/c_2df8fbb1 |
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publishedVersion |
| dc.identifier.citation.spa.fl_str_mv |
Verdugo González L, Acosta Cárdenas A, Zapata Zapata AD, Cadena Chamorro EM, Pulido Muñoz SA. Antimicrobial potential of a hydrolyzed protein extract of the microalgae Nannochloropsis sp. DYNA [Internet]. 1 de octubre de 2019 [citado 28 de junio de 2022];86(211):192-8. Disponible en: https://revistas.unal.edu.co/index.php/dyna/article/view/78865 |
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0012-7353 |
| dc.identifier.uri.none.fl_str_mv |
http://hdl.handle.net/10495/29448 |
| dc.identifier.doi.none.fl_str_mv |
10.15446/dyna.v86n211.78865 |
| dc.identifier.eissn.none.fl_str_mv |
2346-2183 |
| identifier_str_mv |
Verdugo González L, Acosta Cárdenas A, Zapata Zapata AD, Cadena Chamorro EM, Pulido Muñoz SA. Antimicrobial potential of a hydrolyzed protein extract of the microalgae Nannochloropsis sp. DYNA [Internet]. 1 de octubre de 2019 [citado 28 de junio de 2022];86(211):192-8. Disponible en: https://revistas.unal.edu.co/index.php/dyna/article/view/78865 0012-7353 10.15446/dyna.v86n211.78865 2346-2183 |
| url |
http://hdl.handle.net/10495/29448 |
| dc.language.iso.spa.fl_str_mv |
eng |
| language |
eng |
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Dyna |
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info:eu-repo/semantics/openAccess |
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http://creativecommons.org/licenses/by-nc-nd/2.5/co/ |
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openAccess |
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http://creativecommons.org/licenses/by-nc-nd/2.5/co/ http://purl.org/coar/access_right/c_abf2 https://creativecommons.org/licenses/by-nc-nd/4.0/ |
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Universidad Nacional de Colombia, Facultad de Minas, Centro de Publicaciones |
| dc.publisher.group.spa.fl_str_mv |
Grupo de Biotransformación Programa de Estudio y Control de Enfermedades Tropicales (PECET) |
| dc.publisher.place.spa.fl_str_mv |
Medellín, Colombia |
| institution |
Universidad de Antioquia |
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andres.perez@udea.edu.co |
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Verdugo González, LucíaAcosta Cárdenas, AlejandroZapata Zapata, Arley DavidCadena Chamorro, Edith MarlenyPulido Muñoz, Sergio Andrés2022-06-28T23:59:32Z2022-06-28T23:59:32Z2019Verdugo González L, Acosta Cárdenas A, Zapata Zapata AD, Cadena Chamorro EM, Pulido Muñoz SA. Antimicrobial potential of a hydrolyzed protein extract of the microalgae Nannochloropsis sp. DYNA [Internet]. 1 de octubre de 2019 [citado 28 de junio de 2022];86(211):192-8. Disponible en: https://revistas.unal.edu.co/index.php/dyna/article/view/788650012-7353http://hdl.handle.net/10495/2944810.15446/dyna.v86n211.788652346-2183ABSTRACT : Microalgae are photosynthetic microorganisms widely used at the industrial level because of their high content of compounds with bioactive properties such as antimicrobial peptides. These compounds have a wide range of action mechanisms, do not produce the resistance of conventional antibiotics and could be effective against bacteria, fungi, viruses, parasites, and even tumor cells. In this work, by mechanical cell lysis treatment, a protein extract of dry biomass of Nannochloropsis sp was obtained, subsequently, it was hydrolyzed with an enzyme complex of proteases, papain, and pancreatin, for 6 hours, at 44 ° C and pH 7. Finally, the antimicrobial activity of the fraction of hydrolyzed extract with a size less than 3 kDa was evaluated against Staphylococcus aureus, Escherichia coli, and Candida albicans, obtaining a percentage of growth inhibition of 93.1%, 96.6%, and 14.4% respectively, which represents a peptic extract with bacteriostatic and fungistatic properties.RESUMEN : Las microalgas son microorganismos fotosintéticos ampliamente utilizados a nivel industrial gracias a su alto contenido de compuestos con propiedades bioactivas como lo son los péptidos antimicrobianos. Estos compuestos son eficaces contra bacterias, hongos, virus, parásitos e incluso células tumorales, poseen una amplia variedad de mecanismos de acción y no generan la resistencia de los antibióticos convencionales. En este trabajo, a través de lisis celular mecánica, se obtuvo un extracto de proteínas a partir de biomasa seca de Nannochloropsis sp., posteriormente fue hidrolizado usando un complejo enzimático de proteasas, papaína y pancreatina, durante 6 horas, a 44°C y pH 7. Finalmente, se evaluó la actividad antimicrobiana de la fracción de extracto hidrolizado que poseía un tamaño menor a 3 kDa contra Staphylococcus aureus, Escherichia coli y Candida albicans, obteniendo un porcentaje de inhibición de crecimiento de los microorganismos de 93,1%, 96,6%, y 14,4% respectivamente, lo cual representa un extracto peptídico con propiedades bacteriostáticas y fungistáticas.COL0066991COL00150997application/pdfengUniversidad Nacional de Colombia, Facultad de Minas, Centro de PublicacionesGrupo de BiotransformaciónPrograma de Estudio y Control de Enfermedades Tropicales (PECET)Medellín, Colombiainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_2df8fbb1https://purl.org/redcol/resource_type/ARTArtículo de investigaciónhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-nd/2.5/co/http://purl.org/coar/access_right/c_abf2https://creativecommons.org/licenses/by-nc-nd/4.0/Antimicrobial potential of a hydrolyzed protein extract of the microalgae Nannochloropsis sp.Potencial antimicrobiano de un extracto de proteína hidrolizado de la microalga Nannochloropsis sp.HidrólisisHydrolysisProductos con Acción AntimicrobianaProducts with Antimicrobial Actionmicroalgal proteinNannochloropsis sp.Proteína microalgalDynaDyna19219886211ORIGINALVerdugoLucia_2019_Antimicrobial_Potential_Hydrolyzed.pdfVerdugoLucia_2019_Antimicrobial_Potential_Hydrolyzed.pdfArtículo de investigaciónapplication/pdf1978527https://bibliotecadigital.udea.edu.co/bitstream/10495/29448/1/VerdugoLucia_2019_Antimicrobial_Potential_Hydrolyzed.pdf2ac56d48781b53ccead577d67e8509ceMD51CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8823https://bibliotecadigital.udea.edu.co/bitstream/10495/29448/2/license_rdfb88b088d9957e670ce3b3fbe2eedbc13MD52LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://bibliotecadigital.udea.edu.co/bitstream/10495/29448/3/license.txt8a4605be74aa9ea9d79846c1fba20a33MD5310495/29448oai:bibliotecadigital.udea.edu.co:10495/294482022-06-28 18:59:33.107Repositorio Institucional Universidad de Antioquiaandres.perez@udea.edu.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 |