Immunomodulatory activity of itraconazole in lung

ABSTRACT: Purpose: To evaluate the in vivo effects of ITC as an immunomodulator on various immunological mediators, including cytokines, chemokines and growth factors in pulmonary homogenates. Methods: Two experimental groups consisting of 25 BALB/c mice each were used: (a) PBS-treated mice and (b)...

Full description

Autores:
Naranjo Preciado, Tonny Williams
Lopera Henao, Damaris Elena
Zuluaga Salazar, Andrés Felipe
Cano Restrepo, Luz Elena
Tipo de recurso:
Article of investigation
Fecha de publicación:
2016
Institución:
Universidad de Antioquia
Repositorio:
Repositorio UdeA
Idioma:
eng
OAI Identifier:
oai:bibliotecadigital.udea.edu.co:10495/23084
Acceso en línea:
http://hdl.handle.net/10495/23084
Palabra clave:
Inmunomodulación
Immunomodulation
Itraconazol
Itraconazole
Antifúngicos
Antifungal Agents
Histopathological examination
Rights
openAccess
License
http://creativecommons.org/licenses/by/2.5/co/
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oai_identifier_str oai:bibliotecadigital.udea.edu.co:10495/23084
network_acronym_str UDEA2
network_name_str Repositorio UdeA
repository_id_str
dc.title.spa.fl_str_mv Immunomodulatory activity of itraconazole in lung
title Immunomodulatory activity of itraconazole in lung
spellingShingle Immunomodulatory activity of itraconazole in lung
Inmunomodulación
Immunomodulation
Itraconazol
Itraconazole
Antifúngicos
Antifungal Agents
Histopathological examination
title_short Immunomodulatory activity of itraconazole in lung
title_full Immunomodulatory activity of itraconazole in lung
title_fullStr Immunomodulatory activity of itraconazole in lung
title_full_unstemmed Immunomodulatory activity of itraconazole in lung
title_sort Immunomodulatory activity of itraconazole in lung
dc.creator.fl_str_mv Naranjo Preciado, Tonny Williams
Lopera Henao, Damaris Elena
Zuluaga Salazar, Andrés Felipe
Cano Restrepo, Luz Elena
dc.contributor.author.none.fl_str_mv Naranjo Preciado, Tonny Williams
Lopera Henao, Damaris Elena
Zuluaga Salazar, Andrés Felipe
Cano Restrepo, Luz Elena
dc.subject.decs.none.fl_str_mv Inmunomodulación
Immunomodulation
Itraconazol
Itraconazole
Antifúngicos
Antifungal Agents
topic Inmunomodulación
Immunomodulation
Itraconazol
Itraconazole
Antifúngicos
Antifungal Agents
Histopathological examination
dc.subject.proposal.spa.fl_str_mv Histopathological examination
description ABSTRACT: Purpose: To evaluate the in vivo effects of ITC as an immunomodulator on various immunological mediators, including cytokines, chemokines and growth factors in pulmonary homogenates. Methods: Two experimental groups consisting of 25 BALB/c mice each were used: (a) PBS-treated mice and (b) ITC-treated mice (1 mg/mouse/day). The animals were treated daily via gavage for 8 weeks. Five mice from each group were sacrificed at 0, 4, 8, and 12 weeks and cytokine levels assessed in the supernatants of lung macerates using the Bio-Plex system. Five lungs from each experimental group, after 8 weeks of treatment, were used for histopathological analysis. Results: Compared with the control group, ITC-treated mice showed significant changes in the pulmonary levels of 50 % of the molecules evaluated: IL-4, IL-10, IL-12p70, IL-13, TNF-α, eotaxin, MIP- 1α, MIP-2, and LIF were significantly upregulated. In contrast, IL-1β, IL-15, IL-2, IL-1α, and VEGF were downregulated. Conclusion: Although histopathological examination did not show changes in lung cell infiltrates, ITC exerted a marked immunomodulatory effect at the pulmonary level in healthy BALB/c mice.
publishDate 2016
dc.date.issued.none.fl_str_mv 2016
dc.date.accessioned.none.fl_str_mv 2021-10-10T17:28:33Z
dc.date.available.none.fl_str_mv 2021-10-10T17:28:33Z
dc.type.spa.fl_str_mv info:eu-repo/semantics/article
dc.type.coarversion.fl_str_mv http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.hasversion.spa.fl_str_mv info:eu-repo/semantics/publishedVersion
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dc.type.local.spa.fl_str_mv Artículo de investigación
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status_str publishedVersion
dc.identifier.issn.none.fl_str_mv 1596-5996
dc.identifier.uri.none.fl_str_mv http://hdl.handle.net/10495/23084
dc.identifier.doi.none.fl_str_mv 10.4314/tjpr.v15i12.10
dc.identifier.eissn.none.fl_str_mv 1596-9827
identifier_str_mv 1596-5996
10.4314/tjpr.v15i12.10
1596-9827
url http://hdl.handle.net/10495/23084
dc.language.iso.spa.fl_str_mv eng
language eng
dc.relation.ispartofjournalabbrev.spa.fl_str_mv Trop. J. Pharm. Res.
dc.rights.spa.fl_str_mv info:eu-repo/semantics/openAccess
dc.rights.uri.*.fl_str_mv http://creativecommons.org/licenses/by/2.5/co/
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dc.format.extent.spa.fl_str_mv 7
dc.format.mimetype.spa.fl_str_mv application/pdf
dc.publisher.spa.fl_str_mv University of Benin, Faculty of Pharmacy
dc.publisher.group.spa.fl_str_mv GRIPE: Grupo Investigador de Problemas en Enfermedades Infecciosas
Micología Médica y Experimental
dc.publisher.place.spa.fl_str_mv Ciudad de Benín, Nigeria
institution Universidad de Antioquia
bitstream.url.fl_str_mv http://bibliotecadigital.udea.edu.co/bitstream/10495/23084/1/ZuluagaAndres_2016_ImmonomodulatoryItraconazoleLung.pdf
http://bibliotecadigital.udea.edu.co/bitstream/10495/23084/3/license.txt
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spelling Naranjo Preciado, Tonny WilliamsLopera Henao, Damaris ElenaZuluaga Salazar, Andrés FelipeCano Restrepo, Luz Elena2021-10-10T17:28:33Z2021-10-10T17:28:33Z20161596-5996http://hdl.handle.net/10495/2308410.4314/tjpr.v15i12.101596-9827ABSTRACT: Purpose: To evaluate the in vivo effects of ITC as an immunomodulator on various immunological mediators, including cytokines, chemokines and growth factors in pulmonary homogenates. Methods: Two experimental groups consisting of 25 BALB/c mice each were used: (a) PBS-treated mice and (b) ITC-treated mice (1 mg/mouse/day). The animals were treated daily via gavage for 8 weeks. Five mice from each group were sacrificed at 0, 4, 8, and 12 weeks and cytokine levels assessed in the supernatants of lung macerates using the Bio-Plex system. Five lungs from each experimental group, after 8 weeks of treatment, were used for histopathological analysis. Results: Compared with the control group, ITC-treated mice showed significant changes in the pulmonary levels of 50 % of the molecules evaluated: IL-4, IL-10, IL-12p70, IL-13, TNF-α, eotaxin, MIP- 1α, MIP-2, and LIF were significantly upregulated. In contrast, IL-1β, IL-15, IL-2, IL-1α, and VEGF were downregulated. Conclusion: Although histopathological examination did not show changes in lung cell infiltrates, ITC exerted a marked immunomodulatory effect at the pulmonary level in healthy BALB/c mice.COL0005744COL00137097application/pdfengUniversity of Benin, Faculty of PharmacyGRIPE: Grupo Investigador de Problemas en Enfermedades InfecciosasMicología Médica y ExperimentalCiudad de Benín, Nigeriainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_2df8fbb1https://purl.org/redcol/resource_type/ARTArtículo de investigaciónhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/2.5/co/http://purl.org/coar/access_right/c_abf2https://creativecommons.org/licenses/by/4.0/Immunomodulatory activity of itraconazole in lungInmunomodulaciónImmunomodulationItraconazolItraconazoleAntifúngicosAntifungal AgentsHistopathological examinationTrop. J. Pharm. Res.Tropical Journal of Pharmaceutical Research260326091512ORIGINALZuluagaAndres_2016_ImmonomodulatoryItraconazoleLung.pdfZuluagaAndres_2016_ImmonomodulatoryItraconazoleLung.pdfArtículo de investigaciónapplication/pdf234579http://bibliotecadigital.udea.edu.co/bitstream/10495/23084/1/ZuluagaAndres_2016_ImmonomodulatoryItraconazoleLung.pdf3ce2bb7ae83f63bdda1bb1990fae147fMD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748http://bibliotecadigital.udea.edu.co/bitstream/10495/23084/3/license.txt8a4605be74aa9ea9d79846c1fba20a33MD53CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8927http://bibliotecadigital.udea.edu.co/bitstream/10495/23084/2/license_rdf1646d1f6b96dbbbc38035efc9239ac9cMD5210495/23084oai:bibliotecadigital.udea.edu.co:10495/230842021-10-10 12:28:33.604Repositorio Institucional Universidad de Antioquiaandres.perez@udea.edu.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