Escherichia coli lipopolysaccharides decrease molecular expression and activity of disaccharidases and aminopeptidases in weaned pigs

ABSTRACT: Escherichia coli populations in the gut increase after weaning, releasing cell wall lipopolysaccharides (LPS), which are potent pro-inflammatory substances. Objective: this study evaluated LPS intake effects on protein expression and activity of intestinal disaccharidases and aminopetidases. Methods: sixty-four pigs (weaning age: 21 d) were fed a basal diet with added LPS (0.0, 0.3, 0.5, and 1.0 μg LPS/mg feed) during 10 days post-weaning. Pigs were slaughtered on days 1, 5, 7, and 10 post-weaning to assess small intestine molecular expression and enzyme activity. A randomized block design in a 4x4 factorial arrangement was used. Results: intestinal enzyme expression and activity was lower in pigs fed the highest LPS level (p < 0.01). Enzyme values were lowest at day 10 (p < 0.01). The highest gene expression and enzyme activity was observed in duodenum (p < 0.01). Conclusions: data from this study suggest that LPS from E. coli decreases intestinal enzyme expression and activity.

• Autores: Agudelo Trujillo, Jorge Hernán; Parra Suescún, Jaime Eduardo; López Herrera, Albeiro
• Tipo de recurso: Article of investigation
• Fecha de publicación: 2015
• Institución: Universidad de Antioquia
• Repositorio: Repositorio UdeA
• Idioma: eng
• OAI Identifier: oai:bibliotecadigital.udea.edu.co:10495/8005
• Acceso en línea: http://hdl.handle.net/10495/8005
• Palabra clave: Digestive enzymes; Intestinal villi; RT- PCR (Transcripción reversa-PCR); Swine; Escherichia coli; Lipopolisacarido; Cerdos destetados; Enzimas digestivas
• Rights: openAccess
• License: Atribución-NoComercial-CompartirIgual 2.5 Colombia (CC BY-NC-SA 2.5 CO)