High-Resolution Melting (HRM) of the Cytochrome B Gene : A Powerful Approach to Identify Blood-Meal Sources in Chagas Disease Vectors

ABSTRACT: Methods to determine blood-meal sources of hematophagous Triatominae bugs (Chagas disease vectors) are serological or based on PCR employing species-specific primers or heteroduplex analysis, but these are expensive, inaccurate, or problematic when the insect has fed on more than one speci...

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Autores:
Peña García, Víctor Hugo
Fernández García, Geysson Javier
Gómez Palacio, Andrés Mauricio
Mejía Jaramillo, Ana María
Cantillo Barraza, Omar
Triana Chávez, Omar
Tipo de recurso:
Article of investigation
Fecha de publicación:
2012
Institución:
Universidad de Antioquia
Repositorio:
Repositorio UdeA
Idioma:
eng
OAI Identifier:
oai:bibliotecadigital.udea.edu.co:10495/23713
Acceso en línea:
http://hdl.handle.net/10495/23713
Palabra clave:
Citocromos b
Cytochromes b
Enfermedad de Chagas
Chagas Disease
Chinches
Bedbugs
Trypanosoma cruzi
Rights
openAccess
License
http://creativecommons.org/licenses/by/2.5/co/
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oai_identifier_str oai:bibliotecadigital.udea.edu.co:10495/23713
network_acronym_str UDEA2
network_name_str Repositorio UdeA
repository_id_str
dc.title.spa.fl_str_mv High-Resolution Melting (HRM) of the Cytochrome B Gene : A Powerful Approach to Identify Blood-Meal Sources in Chagas Disease Vectors
title High-Resolution Melting (HRM) of the Cytochrome B Gene : A Powerful Approach to Identify Blood-Meal Sources in Chagas Disease Vectors
spellingShingle High-Resolution Melting (HRM) of the Cytochrome B Gene : A Powerful Approach to Identify Blood-Meal Sources in Chagas Disease Vectors
Citocromos b
Cytochromes b
Enfermedad de Chagas
Chagas Disease
Chinches
Bedbugs
Trypanosoma cruzi
title_short High-Resolution Melting (HRM) of the Cytochrome B Gene : A Powerful Approach to Identify Blood-Meal Sources in Chagas Disease Vectors
title_full High-Resolution Melting (HRM) of the Cytochrome B Gene : A Powerful Approach to Identify Blood-Meal Sources in Chagas Disease Vectors
title_fullStr High-Resolution Melting (HRM) of the Cytochrome B Gene : A Powerful Approach to Identify Blood-Meal Sources in Chagas Disease Vectors
title_full_unstemmed High-Resolution Melting (HRM) of the Cytochrome B Gene : A Powerful Approach to Identify Blood-Meal Sources in Chagas Disease Vectors
title_sort High-Resolution Melting (HRM) of the Cytochrome B Gene : A Powerful Approach to Identify Blood-Meal Sources in Chagas Disease Vectors
dc.creator.fl_str_mv Peña García, Víctor Hugo
Fernández García, Geysson Javier
Gómez Palacio, Andrés Mauricio
Mejía Jaramillo, Ana María
Cantillo Barraza, Omar
Triana Chávez, Omar
dc.contributor.author.none.fl_str_mv Peña García, Víctor Hugo
Fernández García, Geysson Javier
Gómez Palacio, Andrés Mauricio
Mejía Jaramillo, Ana María
Cantillo Barraza, Omar
Triana Chávez, Omar
dc.subject.decs.none.fl_str_mv Citocromos b
Cytochromes b
Enfermedad de Chagas
Chagas Disease
Chinches
Bedbugs
Trypanosoma cruzi
topic Citocromos b
Cytochromes b
Enfermedad de Chagas
Chagas Disease
Chinches
Bedbugs
Trypanosoma cruzi
description ABSTRACT: Methods to determine blood-meal sources of hematophagous Triatominae bugs (Chagas disease vectors) are serological or based on PCR employing species-specific primers or heteroduplex analysis, but these are expensive, inaccurate, or problematic when the insect has fed on more than one species. To solve those problems, we developed a technique based on HRM analysis of the mitochondrial gene cytochrome B (Cyt b). This technique recognized 14 species involved in several ecoepidemiological cycles of the transmission of Trypanosoma cruzi and it was suitable with DNA extracted from intestinal content and feces 30 days after feeding, revealing a resolution power that can display mixed feedings. Field samples were analyzed showing blood meal sources corresponding to domestic, peridomiciliary and sylvatic cycles. The technique only requires a single pair of primers that amplify the Cyt b gene in vertebrates and no other standardization, making it quick, easy, relatively inexpensive, and highly accurate.
publishDate 2012
dc.date.issued.none.fl_str_mv 2012
dc.date.accessioned.none.fl_str_mv 2021-11-02T22:54:00Z
dc.date.available.none.fl_str_mv 2021-11-02T22:54:00Z
dc.type.spa.fl_str_mv info:eu-repo/semantics/article
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dc.type.local.spa.fl_str_mv Artículo de investigación
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dc.identifier.citation.spa.fl_str_mv Peña, V., Fernández, G., Gómez A., Mejía, A., Cantillo, O., Triana, O. (2012) High-Resolution Melting (HRM) of the Cytochrome B Gene: A Powerful Approach to Identify Blood-Meal Sources en Vectores de la Enfermedad de Chagas. PLoS Negl Trop Dis 6 (2): e1530. https://doi.org/10.1371/journal.pntd.0001530
dc.identifier.issn.none.fl_str_mv 1935-2727
dc.identifier.uri.none.fl_str_mv http://hdl.handle.net/10495/23713
dc.identifier.doi.none.fl_str_mv 10.1371/journal.pntd.0001530
dc.identifier.eissn.none.fl_str_mv 1935-2735
identifier_str_mv Peña, V., Fernández, G., Gómez A., Mejía, A., Cantillo, O., Triana, O. (2012) High-Resolution Melting (HRM) of the Cytochrome B Gene: A Powerful Approach to Identify Blood-Meal Sources en Vectores de la Enfermedad de Chagas. PLoS Negl Trop Dis 6 (2): e1530. https://doi.org/10.1371/journal.pntd.0001530
1935-2727
10.1371/journal.pntd.0001530
1935-2735
url http://hdl.handle.net/10495/23713
dc.language.iso.spa.fl_str_mv eng
language eng
dc.relation.ispartofjournalabbrev.spa.fl_str_mv PLoS Negl. Trop. Dis.
dc.rights.spa.fl_str_mv info:eu-repo/semantics/openAccess
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dc.rights.creativecommons.spa.fl_str_mv https://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/2.5/co/
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https://creativecommons.org/licenses/by/4.0/
dc.format.extent.spa.fl_str_mv 9
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dc.publisher.spa.fl_str_mv Public Library of Science
dc.publisher.group.spa.fl_str_mv Biología y Control de Enfermedades Infecciosas
dc.publisher.place.spa.fl_str_mv San Francisco, Estados Unidos
institution Universidad de Antioquia
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spelling Peña García, Víctor HugoFernández García, Geysson JavierGómez Palacio, Andrés MauricioMejía Jaramillo, Ana MaríaCantillo Barraza, OmarTriana Chávez, Omar2021-11-02T22:54:00Z2021-11-02T22:54:00Z2012Peña, V., Fernández, G., Gómez A., Mejía, A., Cantillo, O., Triana, O. (2012) High-Resolution Melting (HRM) of the Cytochrome B Gene: A Powerful Approach to Identify Blood-Meal Sources en Vectores de la Enfermedad de Chagas. PLoS Negl Trop Dis 6 (2): e1530. https://doi.org/10.1371/journal.pntd.00015301935-2727http://hdl.handle.net/10495/2371310.1371/journal.pntd.00015301935-2735ABSTRACT: Methods to determine blood-meal sources of hematophagous Triatominae bugs (Chagas disease vectors) are serological or based on PCR employing species-specific primers or heteroduplex analysis, but these are expensive, inaccurate, or problematic when the insect has fed on more than one species. To solve those problems, we developed a technique based on HRM analysis of the mitochondrial gene cytochrome B (Cyt b). This technique recognized 14 species involved in several ecoepidemiological cycles of the transmission of Trypanosoma cruzi and it was suitable with DNA extracted from intestinal content and feces 30 days after feeding, revealing a resolution power that can display mixed feedings. Field samples were analyzed showing blood meal sources corresponding to domestic, peridomiciliary and sylvatic cycles. The technique only requires a single pair of primers that amplify the Cyt b gene in vertebrates and no other standardization, making it quick, easy, relatively inexpensive, and highly accurate.COL00078659application/pdfengPublic Library of ScienceBiología y Control de Enfermedades InfecciosasSan Francisco, Estados Unidosinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_2df8fbb1https://purl.org/redcol/resource_type/ARTArtículo de investigaciónhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/2.5/co/http://purl.org/coar/access_right/c_abf2https://creativecommons.org/licenses/by/4.0/High-Resolution Melting (HRM) of the Cytochrome B Gene : A Powerful Approach to Identify Blood-Meal Sources in Chagas Disease VectorsCitocromos bCytochromes bEnfermedad de ChagasChagas DiseaseChinchesBedbugsTrypanosoma cruziPLoS Negl. Trop. Dis.PLoS Neglected Tropical Diseases1962ORIGINALPeñaVíctor_2012_CytochromeBBloodMeal.pdfPeñaVíctor_2012_CytochromeBBloodMeal.pdfArtículo de investigaciónapplication/pdf559313https://bibliotecadigital.udea.edu.co/bitstream/10495/23713/1/Pe%c3%b1aV%c3%adctor_2012_CytochromeBBloodMeal.pdf6a7a4139107d2054587cdc6fa57ac1baMD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://bibliotecadigital.udea.edu.co/bitstream/10495/23713/3/license.txt8a4605be74aa9ea9d79846c1fba20a33MD53CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8927https://bibliotecadigital.udea.edu.co/bitstream/10495/23713/2/license_rdf1646d1f6b96dbbbc38035efc9239ac9cMD5210495/23713oai:bibliotecadigital.udea.edu.co:10495/237132023-04-11 16:07:27.058Repositorio Institucional Universidad de Antioquiaandres.perez@udea.edu.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