High-Resolution Melting (HRM) of the Cytochrome B Gene : A Powerful Approach to Identify Blood-Meal Sources in Chagas Disease Vectors
ABSTRACT: Methods to determine blood-meal sources of hematophagous Triatominae bugs (Chagas disease vectors) are serological or based on PCR employing species-specific primers or heteroduplex analysis, but these are expensive, inaccurate, or problematic when the insect has fed on more than one speci...
- Autores:
-
Peña García, Víctor Hugo
Fernández García, Geysson Javier
Gómez Palacio, Andrés Mauricio
Mejía Jaramillo, Ana María
Cantillo Barraza, Omar
Triana Chávez, Omar
- Tipo de recurso:
- Article of investigation
- Fecha de publicación:
- 2012
- Institución:
- Universidad de Antioquia
- Repositorio:
- Repositorio UdeA
- Idioma:
- eng
- OAI Identifier:
- oai:bibliotecadigital.udea.edu.co:10495/23713
- Acceso en línea:
- http://hdl.handle.net/10495/23713
- Palabra clave:
- Citocromos b
Cytochromes b
Enfermedad de Chagas
Chagas Disease
Chinches
Bedbugs
Trypanosoma cruzi
- Rights
- openAccess
- License
- http://creativecommons.org/licenses/by/2.5/co/
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|
dc.title.spa.fl_str_mv |
High-Resolution Melting (HRM) of the Cytochrome B Gene : A Powerful Approach to Identify Blood-Meal Sources in Chagas Disease Vectors |
title |
High-Resolution Melting (HRM) of the Cytochrome B Gene : A Powerful Approach to Identify Blood-Meal Sources in Chagas Disease Vectors |
spellingShingle |
High-Resolution Melting (HRM) of the Cytochrome B Gene : A Powerful Approach to Identify Blood-Meal Sources in Chagas Disease Vectors Citocromos b Cytochromes b Enfermedad de Chagas Chagas Disease Chinches Bedbugs Trypanosoma cruzi |
title_short |
High-Resolution Melting (HRM) of the Cytochrome B Gene : A Powerful Approach to Identify Blood-Meal Sources in Chagas Disease Vectors |
title_full |
High-Resolution Melting (HRM) of the Cytochrome B Gene : A Powerful Approach to Identify Blood-Meal Sources in Chagas Disease Vectors |
title_fullStr |
High-Resolution Melting (HRM) of the Cytochrome B Gene : A Powerful Approach to Identify Blood-Meal Sources in Chagas Disease Vectors |
title_full_unstemmed |
High-Resolution Melting (HRM) of the Cytochrome B Gene : A Powerful Approach to Identify Blood-Meal Sources in Chagas Disease Vectors |
title_sort |
High-Resolution Melting (HRM) of the Cytochrome B Gene : A Powerful Approach to Identify Blood-Meal Sources in Chagas Disease Vectors |
dc.creator.fl_str_mv |
Peña García, Víctor Hugo Fernández García, Geysson Javier Gómez Palacio, Andrés Mauricio Mejía Jaramillo, Ana María Cantillo Barraza, Omar Triana Chávez, Omar |
dc.contributor.author.none.fl_str_mv |
Peña García, Víctor Hugo Fernández García, Geysson Javier Gómez Palacio, Andrés Mauricio Mejía Jaramillo, Ana María Cantillo Barraza, Omar Triana Chávez, Omar |
dc.subject.decs.none.fl_str_mv |
Citocromos b Cytochromes b Enfermedad de Chagas Chagas Disease Chinches Bedbugs Trypanosoma cruzi |
topic |
Citocromos b Cytochromes b Enfermedad de Chagas Chagas Disease Chinches Bedbugs Trypanosoma cruzi |
description |
ABSTRACT: Methods to determine blood-meal sources of hematophagous Triatominae bugs (Chagas disease vectors) are serological or based on PCR employing species-specific primers or heteroduplex analysis, but these are expensive, inaccurate, or problematic when the insect has fed on more than one species. To solve those problems, we developed a technique based on HRM analysis of the mitochondrial gene cytochrome B (Cyt b). This technique recognized 14 species involved in several ecoepidemiological cycles of the transmission of Trypanosoma cruzi and it was suitable with DNA extracted from intestinal content and feces 30 days after feeding, revealing a resolution power that can display mixed feedings. Field samples were analyzed showing blood meal sources corresponding to domestic, peridomiciliary and sylvatic cycles. The technique only requires a single pair of primers that amplify the Cyt b gene in vertebrates and no other standardization, making it quick, easy, relatively inexpensive, and highly accurate. |
publishDate |
2012 |
dc.date.issued.none.fl_str_mv |
2012 |
dc.date.accessioned.none.fl_str_mv |
2021-11-02T22:54:00Z |
dc.date.available.none.fl_str_mv |
2021-11-02T22:54:00Z |
dc.type.spa.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.coarversion.fl_str_mv |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
dc.type.hasversion.spa.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.coar.spa.fl_str_mv |
http://purl.org/coar/resource_type/c_2df8fbb1 |
dc.type.redcol.spa.fl_str_mv |
https://purl.org/redcol/resource_type/ART |
dc.type.local.spa.fl_str_mv |
Artículo de investigación |
format |
http://purl.org/coar/resource_type/c_2df8fbb1 |
status_str |
publishedVersion |
dc.identifier.citation.spa.fl_str_mv |
Peña, V., Fernández, G., Gómez A., Mejía, A., Cantillo, O., Triana, O. (2012) High-Resolution Melting (HRM) of the Cytochrome B Gene: A Powerful Approach to Identify Blood-Meal Sources en Vectores de la Enfermedad de Chagas. PLoS Negl Trop Dis 6 (2): e1530. https://doi.org/10.1371/journal.pntd.0001530 |
dc.identifier.issn.none.fl_str_mv |
1935-2727 |
dc.identifier.uri.none.fl_str_mv |
http://hdl.handle.net/10495/23713 |
dc.identifier.doi.none.fl_str_mv |
10.1371/journal.pntd.0001530 |
dc.identifier.eissn.none.fl_str_mv |
1935-2735 |
identifier_str_mv |
Peña, V., Fernández, G., Gómez A., Mejía, A., Cantillo, O., Triana, O. (2012) High-Resolution Melting (HRM) of the Cytochrome B Gene: A Powerful Approach to Identify Blood-Meal Sources en Vectores de la Enfermedad de Chagas. PLoS Negl Trop Dis 6 (2): e1530. https://doi.org/10.1371/journal.pntd.0001530 1935-2727 10.1371/journal.pntd.0001530 1935-2735 |
url |
http://hdl.handle.net/10495/23713 |
dc.language.iso.spa.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartofjournalabbrev.spa.fl_str_mv |
PLoS Negl. Trop. Dis. |
dc.rights.spa.fl_str_mv |
info:eu-repo/semantics/openAccess |
dc.rights.uri.*.fl_str_mv |
http://creativecommons.org/licenses/by/2.5/co/ |
dc.rights.accessrights.spa.fl_str_mv |
http://purl.org/coar/access_right/c_abf2 |
dc.rights.creativecommons.spa.fl_str_mv |
https://creativecommons.org/licenses/by/4.0/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by/2.5/co/ http://purl.org/coar/access_right/c_abf2 https://creativecommons.org/licenses/by/4.0/ |
dc.format.extent.spa.fl_str_mv |
9 |
dc.format.mimetype.spa.fl_str_mv |
application/pdf |
dc.publisher.spa.fl_str_mv |
Public Library of Science |
dc.publisher.group.spa.fl_str_mv |
Biología y Control de Enfermedades Infecciosas |
dc.publisher.place.spa.fl_str_mv |
San Francisco, Estados Unidos |
institution |
Universidad de Antioquia |
bitstream.url.fl_str_mv |
https://bibliotecadigital.udea.edu.co/bitstream/10495/23713/1/Pe%c3%b1aV%c3%adctor_2012_CytochromeBBloodMeal.pdf https://bibliotecadigital.udea.edu.co/bitstream/10495/23713/3/license.txt https://bibliotecadigital.udea.edu.co/bitstream/10495/23713/2/license_rdf |
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MD5 MD5 MD5 |
repository.name.fl_str_mv |
Repositorio Institucional Universidad de Antioquia |
repository.mail.fl_str_mv |
andres.perez@udea.edu.co |
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1812173288875491328 |
spelling |
Peña García, Víctor HugoFernández García, Geysson JavierGómez Palacio, Andrés MauricioMejía Jaramillo, Ana MaríaCantillo Barraza, OmarTriana Chávez, Omar2021-11-02T22:54:00Z2021-11-02T22:54:00Z2012Peña, V., Fernández, G., Gómez A., Mejía, A., Cantillo, O., Triana, O. (2012) High-Resolution Melting (HRM) of the Cytochrome B Gene: A Powerful Approach to Identify Blood-Meal Sources en Vectores de la Enfermedad de Chagas. PLoS Negl Trop Dis 6 (2): e1530. https://doi.org/10.1371/journal.pntd.00015301935-2727http://hdl.handle.net/10495/2371310.1371/journal.pntd.00015301935-2735ABSTRACT: Methods to determine blood-meal sources of hematophagous Triatominae bugs (Chagas disease vectors) are serological or based on PCR employing species-specific primers or heteroduplex analysis, but these are expensive, inaccurate, or problematic when the insect has fed on more than one species. To solve those problems, we developed a technique based on HRM analysis of the mitochondrial gene cytochrome B (Cyt b). This technique recognized 14 species involved in several ecoepidemiological cycles of the transmission of Trypanosoma cruzi and it was suitable with DNA extracted from intestinal content and feces 30 days after feeding, revealing a resolution power that can display mixed feedings. Field samples were analyzed showing blood meal sources corresponding to domestic, peridomiciliary and sylvatic cycles. The technique only requires a single pair of primers that amplify the Cyt b gene in vertebrates and no other standardization, making it quick, easy, relatively inexpensive, and highly accurate.COL00078659application/pdfengPublic Library of ScienceBiología y Control de Enfermedades InfecciosasSan Francisco, Estados Unidosinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_2df8fbb1https://purl.org/redcol/resource_type/ARTArtículo de investigaciónhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/2.5/co/http://purl.org/coar/access_right/c_abf2https://creativecommons.org/licenses/by/4.0/High-Resolution Melting (HRM) of the Cytochrome B Gene : A Powerful Approach to Identify Blood-Meal Sources in Chagas Disease VectorsCitocromos bCytochromes bEnfermedad de ChagasChagas DiseaseChinchesBedbugsTrypanosoma cruziPLoS Negl. Trop. Dis.PLoS Neglected Tropical Diseases1962ORIGINALPeñaVíctor_2012_CytochromeBBloodMeal.pdfPeñaVíctor_2012_CytochromeBBloodMeal.pdfArtículo de investigaciónapplication/pdf559313https://bibliotecadigital.udea.edu.co/bitstream/10495/23713/1/Pe%c3%b1aV%c3%adctor_2012_CytochromeBBloodMeal.pdf6a7a4139107d2054587cdc6fa57ac1baMD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://bibliotecadigital.udea.edu.co/bitstream/10495/23713/3/license.txt8a4605be74aa9ea9d79846c1fba20a33MD53CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8927https://bibliotecadigital.udea.edu.co/bitstream/10495/23713/2/license_rdf1646d1f6b96dbbbc38035efc9239ac9cMD5210495/23713oai:bibliotecadigital.udea.edu.co:10495/237132023-04-11 16:07:27.058Repositorio Institucional Universidad de Antioquiaandres.perez@udea.edu.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 |