High-Resolution Melting (HRM) of the Cytochrome B Gene : A Powerful Approach to Identify Blood-Meal Sources in Chagas Disease Vectors
ABSTRACT: Methods to determine blood-meal sources of hematophagous Triatominae bugs (Chagas disease vectors) are serological or based on PCR employing species-specific primers or heteroduplex analysis, but these are expensive, inaccurate, or problematic when the insect has fed on more than one speci...
- Autores:
-
Peña García, Víctor Hugo
Fernández García, Geysson Javier
Gómez Palacio, Andrés Mauricio
Mejía Jaramillo, Ana María
Cantillo Barraza, Omar
Triana Chávez, Omar
- Tipo de recurso:
- Article of investigation
- Fecha de publicación:
- 2012
- Institución:
- Universidad de Antioquia
- Repositorio:
- Repositorio UdeA
- Idioma:
- eng
- OAI Identifier:
- oai:bibliotecadigital.udea.edu.co:10495/23713
- Acceso en línea:
- http://hdl.handle.net/10495/23713
- Palabra clave:
- Chinches
Bedbugs
Trypanosoma cruzi
Hemípteros
Hemiptera
Análisis heterodúplex
Heteroduplex Analysis
Citocromos B
Cytochromes B
Enfermedad de Chagas
Chagas Disease
ADN mitocondrial
Mitochondrial DNA
Vectores de la enfermedad de Chagas
https://id.nlm.nih.gov/mesh/D001511
https://id.nlm.nih.gov/mesh/D014349
https://id.nlm.nih.gov/mesh/D006430
https://id.nlm.nih.gov/mesh/D020180
https://id.nlm.nih.gov/mesh/D045303
https://id.nlm.nih.gov/mesh/D014355
- Rights
- openAccess
- License
- http://creativecommons.org/licenses/by/2.5/co/
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| dc.title.spa.fl_str_mv |
High-Resolution Melting (HRM) of the Cytochrome B Gene : A Powerful Approach to Identify Blood-Meal Sources in Chagas Disease Vectors |
| title |
High-Resolution Melting (HRM) of the Cytochrome B Gene : A Powerful Approach to Identify Blood-Meal Sources in Chagas Disease Vectors |
| spellingShingle |
High-Resolution Melting (HRM) of the Cytochrome B Gene : A Powerful Approach to Identify Blood-Meal Sources in Chagas Disease Vectors Chinches Bedbugs Trypanosoma cruzi Hemípteros Hemiptera Análisis heterodúplex Heteroduplex Analysis Citocromos B Cytochromes B Enfermedad de Chagas Chagas Disease ADN mitocondrial Mitochondrial DNA Vectores de la enfermedad de Chagas https://id.nlm.nih.gov/mesh/D001511 https://id.nlm.nih.gov/mesh/D014349 https://id.nlm.nih.gov/mesh/D006430 https://id.nlm.nih.gov/mesh/D020180 https://id.nlm.nih.gov/mesh/D045303 https://id.nlm.nih.gov/mesh/D014355 |
| title_short |
High-Resolution Melting (HRM) of the Cytochrome B Gene : A Powerful Approach to Identify Blood-Meal Sources in Chagas Disease Vectors |
| title_full |
High-Resolution Melting (HRM) of the Cytochrome B Gene : A Powerful Approach to Identify Blood-Meal Sources in Chagas Disease Vectors |
| title_fullStr |
High-Resolution Melting (HRM) of the Cytochrome B Gene : A Powerful Approach to Identify Blood-Meal Sources in Chagas Disease Vectors |
| title_full_unstemmed |
High-Resolution Melting (HRM) of the Cytochrome B Gene : A Powerful Approach to Identify Blood-Meal Sources in Chagas Disease Vectors |
| title_sort |
High-Resolution Melting (HRM) of the Cytochrome B Gene : A Powerful Approach to Identify Blood-Meal Sources in Chagas Disease Vectors |
| dc.creator.fl_str_mv |
Peña García, Víctor Hugo Fernández García, Geysson Javier Gómez Palacio, Andrés Mauricio Mejía Jaramillo, Ana María Cantillo Barraza, Omar Triana Chávez, Omar |
| dc.contributor.author.none.fl_str_mv |
Peña García, Víctor Hugo Fernández García, Geysson Javier Gómez Palacio, Andrés Mauricio Mejía Jaramillo, Ana María Cantillo Barraza, Omar Triana Chávez, Omar |
| dc.contributor.researchgroup.spa.fl_str_mv |
Biología y Control de Enfermedades Infecciosas |
| dc.subject.decs.none.fl_str_mv |
Chinches Bedbugs Trypanosoma cruzi Hemípteros Hemiptera Análisis heterodúplex Heteroduplex Analysis Citocromos B Cytochromes B Enfermedad de Chagas Chagas Disease |
| topic |
Chinches Bedbugs Trypanosoma cruzi Hemípteros Hemiptera Análisis heterodúplex Heteroduplex Analysis Citocromos B Cytochromes B Enfermedad de Chagas Chagas Disease ADN mitocondrial Mitochondrial DNA Vectores de la enfermedad de Chagas https://id.nlm.nih.gov/mesh/D001511 https://id.nlm.nih.gov/mesh/D014349 https://id.nlm.nih.gov/mesh/D006430 https://id.nlm.nih.gov/mesh/D020180 https://id.nlm.nih.gov/mesh/D045303 https://id.nlm.nih.gov/mesh/D014355 |
| dc.subject.lemb.none.fl_str_mv |
ADN mitocondrial Mitochondrial DNA |
| dc.subject.proposal.spa.fl_str_mv |
Vectores de la enfermedad de Chagas |
| dc.subject.meshuri.none.fl_str_mv |
https://id.nlm.nih.gov/mesh/D001511 https://id.nlm.nih.gov/mesh/D014349 https://id.nlm.nih.gov/mesh/D006430 https://id.nlm.nih.gov/mesh/D020180 https://id.nlm.nih.gov/mesh/D045303 https://id.nlm.nih.gov/mesh/D014355 |
| description |
ABSTRACT: Methods to determine blood-meal sources of hematophagous Triatominae bugs (Chagas disease vectors) are serological or based on PCR employing species-specific primers or heteroduplex analysis, but these are expensive, inaccurate, or problematic when the insect has fed on more than one species. To solve those problems, we developed a technique based on HRM analysis of the mitochondrial gene cytochrome B (Cyt b). This technique recognized 14 species involved in several ecoepidemiological cycles of the transmission of Trypanosoma cruzi and it was suitable with DNA extracted from intestinal content and feces 30 days after feeding, revealing a resolution power that can display mixed feedings. Field samples were analyzed showing blood meal sources corresponding to domestic, peridomiciliary and sylvatic cycles. The technique only requires a single pair of primers that amplify the Cyt b gene in vertebrates and no other standardization, making it quick, easy, relatively inexpensive, and highly accurate. |
| publishDate |
2012 |
| dc.date.issued.none.fl_str_mv |
2012 |
| dc.date.accessioned.none.fl_str_mv |
2021-11-02T22:54:00Z |
| dc.date.available.none.fl_str_mv |
2021-11-02T22:54:00Z |
| dc.type.spa.fl_str_mv |
Artículo de investigación |
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http://purl.org/coar/resource_type/c_2df8fbb1 |
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https://purl.org/redcol/resource_type/ART |
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http://purl.org/coar/version/c_970fb48d4fbd8a85 |
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info:eu-repo/semantics/article |
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info:eu-repo/semantics/publishedVersion |
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http://purl.org/coar/resource_type/c_2df8fbb1 |
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publishedVersion |
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Peña, V., Fernández, G., Gómez A., Mejía, A., Cantillo, O., Triana, O. (2012) High-Resolution Melting (HRM) of the Cytochrome B Gene: A Powerful Approach to Identify Blood-Meal Sources en Vectores de la Enfermedad de Chagas. PLoS Negl Trop Dis 6 (2): e1530. https://doi.org/10.1371/journal.pntd.0001530 |
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1935-2727 |
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http://hdl.handle.net/10495/23713 |
| dc.identifier.doi.none.fl_str_mv |
10.1371/journal.pntd.0001530 |
| dc.identifier.eissn.none.fl_str_mv |
1935-2735 |
| identifier_str_mv |
Peña, V., Fernández, G., Gómez A., Mejía, A., Cantillo, O., Triana, O. (2012) High-Resolution Melting (HRM) of the Cytochrome B Gene: A Powerful Approach to Identify Blood-Meal Sources en Vectores de la Enfermedad de Chagas. PLoS Negl Trop Dis 6 (2): e1530. https://doi.org/10.1371/journal.pntd.0001530 1935-2727 10.1371/journal.pntd.0001530 1935-2735 |
| url |
http://hdl.handle.net/10495/23713 |
| dc.language.iso.spa.fl_str_mv |
eng |
| language |
eng |
| dc.relation.ispartofjournalabbrev.spa.fl_str_mv |
PLoS Negl. Trop. Dis. |
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9 |
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2 |
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1 |
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PLoS Neglected Tropical Diseases |
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Peña García, Víctor HugoFernández García, Geysson JavierGómez Palacio, Andrés MauricioMejía Jaramillo, Ana MaríaCantillo Barraza, OmarTriana Chávez, OmarBiología y Control de Enfermedades Infecciosas2021-11-02T22:54:00Z2021-11-02T22:54:00Z2012Peña, V., Fernández, G., Gómez A., Mejía, A., Cantillo, O., Triana, O. (2012) High-Resolution Melting (HRM) of the Cytochrome B Gene: A Powerful Approach to Identify Blood-Meal Sources en Vectores de la Enfermedad de Chagas. PLoS Negl Trop Dis 6 (2): e1530. https://doi.org/10.1371/journal.pntd.00015301935-2727http://hdl.handle.net/10495/2371310.1371/journal.pntd.00015301935-2735ABSTRACT: Methods to determine blood-meal sources of hematophagous Triatominae bugs (Chagas disease vectors) are serological or based on PCR employing species-specific primers or heteroduplex analysis, but these are expensive, inaccurate, or problematic when the insect has fed on more than one species. To solve those problems, we developed a technique based on HRM analysis of the mitochondrial gene cytochrome B (Cyt b). This technique recognized 14 species involved in several ecoepidemiological cycles of the transmission of Trypanosoma cruzi and it was suitable with DNA extracted from intestinal content and feces 30 days after feeding, revealing a resolution power that can display mixed feedings. Field samples were analyzed showing blood meal sources corresponding to domestic, peridomiciliary and sylvatic cycles. The technique only requires a single pair of primers that amplify the Cyt b gene in vertebrates and no other standardization, making it quick, easy, relatively inexpensive, and highly accurate.COL00078659application/pdfengPublic Library of ScienceSan Francisco, Estados Unidoshttp://creativecommons.org/licenses/by/2.5/co/https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2High-Resolution Melting (HRM) of the Cytochrome B Gene : A Powerful Approach to Identify Blood-Meal Sources in Chagas Disease VectorsArtículo de investigaciónhttp://purl.org/coar/resource_type/c_2df8fbb1https://purl.org/redcol/resource_type/ARThttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionChinchesBedbugsTrypanosoma cruziHemípterosHemipteraAnálisis heterodúplexHeteroduplex AnalysisCitocromos BCytochromes BEnfermedad de ChagasChagas DiseaseADN mitocondrialMitochondrial DNAVectores de la enfermedad de Chagashttps://id.nlm.nih.gov/mesh/D001511https://id.nlm.nih.gov/mesh/D014349https://id.nlm.nih.gov/mesh/D006430https://id.nlm.nih.gov/mesh/D020180https://id.nlm.nih.gov/mesh/D045303https://id.nlm.nih.gov/mesh/D014355PLoS Negl. Trop. 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