Ex situ potential conservation of ipecacuanha (Psychotria ipecacuanha (Brot.) Stokes.), a critically endangered medicinal plant species
ABSTRACT: Psychotria ipecacuanha is a medicinal plant endangered in Colombia, due to the indiscriminate exploitation suffered several decades ago for its therapeutic importance. Its great medicinal value is due to emetine production and its derivatives, mainly in the roots. Given these concerns, the...
- Autores:
-
Urrea Trujillo, Aura Inés
Botero Giraldo, Catalina
Naranjo Gómez, Esther Julia
- Tipo de recurso:
- Article of investigation
- Fecha de publicación:
- 2017
- Institución:
- Universidad de Antioquia
- Repositorio:
- Repositorio UdeA
- Idioma:
- eng
- OAI Identifier:
- oai:bibliotecadigital.udea.edu.co:10495/20373
- Acceso en línea:
- http://hdl.handle.net/10495/20373
- Palabra clave:
- Vigour
Vigor
In vitro
In vitro conservation
Plant limited growth
Osmotic regulator
Recovery phase
http://aims.fao.org/aos/agrovoc/c_37512
http://aims.fao.org/aos/agrovoc/c_37563
- Rights
- openAccess
- License
- http://creativecommons.org/licenses/by-nc-nd/2.5/co/
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dc.title.spa.fl_str_mv |
Ex situ potential conservation of ipecacuanha (Psychotria ipecacuanha (Brot.) Stokes.), a critically endangered medicinal plant species |
dc.title.alternative.spa.fl_str_mv |
Potencial de conservación ex situ de ipecacuana (Psychotria ipecacuanha (Brot.) Stokes.), una planta medicinal en peligro crítico de extinción |
title |
Ex situ potential conservation of ipecacuanha (Psychotria ipecacuanha (Brot.) Stokes.), a critically endangered medicinal plant species |
spellingShingle |
Ex situ potential conservation of ipecacuanha (Psychotria ipecacuanha (Brot.) Stokes.), a critically endangered medicinal plant species Vigour Vigor In vitro In vitro conservation Plant limited growth Osmotic regulator Recovery phase http://aims.fao.org/aos/agrovoc/c_37512 http://aims.fao.org/aos/agrovoc/c_37563 |
title_short |
Ex situ potential conservation of ipecacuanha (Psychotria ipecacuanha (Brot.) Stokes.), a critically endangered medicinal plant species |
title_full |
Ex situ potential conservation of ipecacuanha (Psychotria ipecacuanha (Brot.) Stokes.), a critically endangered medicinal plant species |
title_fullStr |
Ex situ potential conservation of ipecacuanha (Psychotria ipecacuanha (Brot.) Stokes.), a critically endangered medicinal plant species |
title_full_unstemmed |
Ex situ potential conservation of ipecacuanha (Psychotria ipecacuanha (Brot.) Stokes.), a critically endangered medicinal plant species |
title_sort |
Ex situ potential conservation of ipecacuanha (Psychotria ipecacuanha (Brot.) Stokes.), a critically endangered medicinal plant species |
dc.creator.fl_str_mv |
Urrea Trujillo, Aura Inés Botero Giraldo, Catalina Naranjo Gómez, Esther Julia |
dc.contributor.author.none.fl_str_mv |
Urrea Trujillo, Aura Inés Botero Giraldo, Catalina Naranjo Gómez, Esther Julia |
dc.subject.agrovoc.none.fl_str_mv |
Vigour Vigor In vitro |
topic |
Vigour Vigor In vitro In vitro conservation Plant limited growth Osmotic regulator Recovery phase http://aims.fao.org/aos/agrovoc/c_37512 http://aims.fao.org/aos/agrovoc/c_37563 |
dc.subject.proposal.spa.fl_str_mv |
In vitro conservation Plant limited growth Osmotic regulator Recovery phase |
dc.subject.agrovocuri.none.fl_str_mv |
http://aims.fao.org/aos/agrovoc/c_37512 http://aims.fao.org/aos/agrovoc/c_37563 |
description |
ABSTRACT: Psychotria ipecacuanha is a medicinal plant endangered in Colombia, due to the indiscriminate exploitation suffered several decades ago for its therapeutic importance. Its great medicinal value is due to emetine production and its derivatives, mainly in the roots. Given these concerns, the aim of this research was to evaluate different parameters for in vitro conservation of P. ipecacuanha by a limited plant growth, using as in vitro explants plant nodes. Plant growth response to 18, 23 and 25°C with and without addition to MS basal culture medium 15 and 30 gL-1 of mannitol osmotic regulator was evaluated. Registration of plant survival data (%), plant height (cm), plant development of axillary shoots, number of leaves, callus formation and vigour of developed buds and was initially performed at 3 months and then every two months until 12 months. The explants conserved for 9 months in culture medium without mannitol osmotic regulator and temperature of 18°C, showed response after transferring and reculturing in recovery culture medium for 6-8 weeks, this process had not achieved any effect on plant growth and vigour in the recovery phase. In addition, the critical parameters in the acclimatization process were determined. |
publishDate |
2017 |
dc.date.issued.none.fl_str_mv |
2017 |
dc.date.accessioned.none.fl_str_mv |
2021-06-25T03:22:06Z |
dc.date.available.none.fl_str_mv |
2021-06-25T03:22:06Z |
dc.type.spa.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.coarversion.fl_str_mv |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
dc.type.hasversion.spa.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.coar.spa.fl_str_mv |
http://purl.org/coar/resource_type/c_2df8fbb1 |
dc.type.redcol.spa.fl_str_mv |
https://purl.org/redcol/resource_type/ART |
dc.type.local.spa.fl_str_mv |
Artículo de investigación |
format |
http://purl.org/coar/resource_type/c_2df8fbb1 |
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publishedVersion |
dc.identifier.citation.spa.fl_str_mv |
Urrea Trujillo, A. I., Botero Giraldo, C., & Naranjo Gómez, E. J. (2017). Ex situ potential conservation of ipecacuanha (Psychotria ipecacuanha (Brot.) Stokes.), a critically endangered medicinal plant species. Acta Agronómica, 66(4), 598 - 605. https://doi.org/10.15446/acag.v66n4.60080 |
dc.identifier.issn.none.fl_str_mv |
0120-2812 |
dc.identifier.uri.none.fl_str_mv |
http://hdl.handle.net/10495/20373 |
dc.identifier.doi.none.fl_str_mv |
10.15446/acag.v66n4.60080 |
dc.identifier.eissn.none.fl_str_mv |
2323-0118 |
identifier_str_mv |
Urrea Trujillo, A. I., Botero Giraldo, C., & Naranjo Gómez, E. J. (2017). Ex situ potential conservation of ipecacuanha (Psychotria ipecacuanha (Brot.) Stokes.), a critically endangered medicinal plant species. Acta Agronómica, 66(4), 598 - 605. https://doi.org/10.15446/acag.v66n4.60080 0120-2812 10.15446/acag.v66n4.60080 2323-0118 |
url |
http://hdl.handle.net/10495/20373 |
dc.language.iso.spa.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartofjournalabbrev.spa.fl_str_mv |
Acta agron. |
dc.rights.spa.fl_str_mv |
info:eu-repo/semantics/openAccess |
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http://creativecommons.org/licenses/by-nc-nd/2.5/co/ |
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https://creativecommons.org/licenses/by-nc-nd/4.0/ |
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openAccess |
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http://creativecommons.org/licenses/by-nc-nd/2.5/co/ http://purl.org/coar/access_right/c_abf2 https://creativecommons.org/licenses/by-nc-nd/4.0/ |
dc.format.extent.spa.fl_str_mv |
8 |
dc.format.mimetype.spa.fl_str_mv |
application/pdf |
dc.publisher.spa.fl_str_mv |
Universidad Nacional de Colombia |
dc.publisher.group.spa.fl_str_mv |
Biotecnología |
dc.publisher.place.spa.fl_str_mv |
Palmira, Colombia |
institution |
Universidad de Antioquia |
bitstream.url.fl_str_mv |
http://bibliotecadigital.udea.edu.co/bitstream/10495/20373/1/UrreaAura_2017_ConservationIpecacuanha.pdf http://bibliotecadigital.udea.edu.co/bitstream/10495/20373/3/license.txt http://bibliotecadigital.udea.edu.co/bitstream/10495/20373/2/license_rdf |
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andres.perez@udea.edu.co |
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1812173098818994176 |
spelling |
Urrea Trujillo, Aura InésBotero Giraldo, CatalinaNaranjo Gómez, Esther Julia2021-06-25T03:22:06Z2021-06-25T03:22:06Z2017Urrea Trujillo, A. I., Botero Giraldo, C., & Naranjo Gómez, E. J. (2017). Ex situ potential conservation of ipecacuanha (Psychotria ipecacuanha (Brot.) Stokes.), a critically endangered medicinal plant species. Acta Agronómica, 66(4), 598 - 605. https://doi.org/10.15446/acag.v66n4.600800120-2812http://hdl.handle.net/10495/2037310.15446/acag.v66n4.600802323-0118ABSTRACT: Psychotria ipecacuanha is a medicinal plant endangered in Colombia, due to the indiscriminate exploitation suffered several decades ago for its therapeutic importance. Its great medicinal value is due to emetine production and its derivatives, mainly in the roots. Given these concerns, the aim of this research was to evaluate different parameters for in vitro conservation of P. ipecacuanha by a limited plant growth, using as in vitro explants plant nodes. Plant growth response to 18, 23 and 25°C with and without addition to MS basal culture medium 15 and 30 gL-1 of mannitol osmotic regulator was evaluated. Registration of plant survival data (%), plant height (cm), plant development of axillary shoots, number of leaves, callus formation and vigour of developed buds and was initially performed at 3 months and then every two months until 12 months. The explants conserved for 9 months in culture medium without mannitol osmotic regulator and temperature of 18°C, showed response after transferring and reculturing in recovery culture medium for 6-8 weeks, this process had not achieved any effect on plant growth and vigour in the recovery phase. In addition, the critical parameters in the acclimatization process were determined.RESUMEN: Psychotria ipecacuanha es una especie medicinal amenazada en Colombia, debido a la explotación indiscriminada que varias décadas atrás sufrió por su importancia terapéutica. Su gran valor medicinal se debe a la producción de emetina y sus derivados, principalmente en las raíces. Ante este panorama, el presente estudio tuvo como objetivo evaluar diferentes parámetros para la conservación in vitro de P. ipecacuanha por crecimiento limitado, usando como explantes nudos de plantas in vitro. Se evaluó la respuesta de crecimiento a 18, 23 y 25°C sin y con la adición al medio de cultivo basal MS del regulador osmótico manitol a 15 y 30 gL-1. El registro de los datos de supervivencia (%), altura (cm), desarrollo de brotes axilares, número de hojas, formación de callo y vigor de los brotes desarrollados, se realizó inicialmente a los 3 meses y luego cada dos meses hasta completar 12 meses. Una temperatura de 18°C, sin la adición del regulador osmótico manitol al medio cultivo basal MS, permitió mantener vitroplantas de esta especie hasta por nueve meses, sin verse afectado el crecimiento y vigor en la etapa de recuperación. Además se logró determinar los parámetros críticos en el proceso de aclimatización.COL00010848application/pdfengUniversidad Nacional de ColombiaBiotecnologíaPalmira, Colombiainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_2df8fbb1https://purl.org/redcol/resource_type/ARTArtículo de investigaciónhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-nd/2.5/co/http://purl.org/coar/access_right/c_abf2https://creativecommons.org/licenses/by-nc-nd/4.0/Ex situ potential conservation of ipecacuanha (Psychotria ipecacuanha (Brot.) Stokes.), a critically endangered medicinal plant speciesPotencial de conservación ex situ de ipecacuana (Psychotria ipecacuanha (Brot.) Stokes.), una planta medicinal en peligro crítico de extinciónVigourVigorIn vitroIn vitro conservationPlant limited growthOsmotic regulatorRecovery phasehttp://aims.fao.org/aos/agrovoc/c_37512http://aims.fao.org/aos/agrovoc/c_37563Acta agron.Acta Agronómica598605664ORIGINALUrreaAura_2017_ConservationIpecacuanha.pdfUrreaAura_2017_ConservationIpecacuanha.pdfArtículo de investigaciónapplication/pdf33149605http://bibliotecadigital.udea.edu.co/bitstream/10495/20373/1/UrreaAura_2017_ConservationIpecacuanha.pdfdccc331e9743bc794df8b5f1d7dd5f4fMD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748http://bibliotecadigital.udea.edu.co/bitstream/10495/20373/3/license.txt8a4605be74aa9ea9d79846c1fba20a33MD53CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8823http://bibliotecadigital.udea.edu.co/bitstream/10495/20373/2/license_rdfb88b088d9957e670ce3b3fbe2eedbc13MD5210495/20373oai:bibliotecadigital.udea.edu.co:10495/203732021-06-24 22:22:07.134Repositorio Institucional Universidad de Antioquiaandres.perez@udea.edu.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 |