Ex situ potential conservation of ipecacuanha (Psychotria ipecacuanha (Brot.) Stokes.), a critically endangered medicinal plant species

ABSTRACT: Psychotria ipecacuanha is a medicinal plant endangered in Colombia, due to the indiscriminate exploitation suffered several decades ago for its therapeutic importance. Its great medicinal value is due to emetine production and its derivatives, mainly in the roots. Given these concerns, the...

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Autores:
Urrea Trujillo, Aura Inés
Botero Giraldo, Catalina
Naranjo Gómez, Esther Julia
Tipo de recurso:
Article of investigation
Fecha de publicación:
2017
Institución:
Universidad de Antioquia
Repositorio:
Repositorio UdeA
Idioma:
eng
OAI Identifier:
oai:bibliotecadigital.udea.edu.co:10495/20373
Acceso en línea:
http://hdl.handle.net/10495/20373
Palabra clave:
Vigour
Vigor
In vitro
In vitro conservation
Plant limited growth
Osmotic regulator
Recovery phase
http://aims.fao.org/aos/agrovoc/c_37512
http://aims.fao.org/aos/agrovoc/c_37563
Rights
openAccess
License
http://creativecommons.org/licenses/by-nc-nd/2.5/co/
id UDEA2_64985047229db0b068072160557f0868
oai_identifier_str oai:bibliotecadigital.udea.edu.co:10495/20373
network_acronym_str UDEA2
network_name_str Repositorio UdeA
repository_id_str
dc.title.spa.fl_str_mv Ex situ potential conservation of ipecacuanha (Psychotria ipecacuanha (Brot.) Stokes.), a critically endangered medicinal plant species
dc.title.alternative.spa.fl_str_mv Potencial de conservación ex situ de ipecacuana (Psychotria ipecacuanha (Brot.) Stokes.), una planta medicinal en peligro crítico de extinción
title Ex situ potential conservation of ipecacuanha (Psychotria ipecacuanha (Brot.) Stokes.), a critically endangered medicinal plant species
spellingShingle Ex situ potential conservation of ipecacuanha (Psychotria ipecacuanha (Brot.) Stokes.), a critically endangered medicinal plant species
Vigour
Vigor
In vitro
In vitro conservation
Plant limited growth
Osmotic regulator
Recovery phase
http://aims.fao.org/aos/agrovoc/c_37512
http://aims.fao.org/aos/agrovoc/c_37563
title_short Ex situ potential conservation of ipecacuanha (Psychotria ipecacuanha (Brot.) Stokes.), a critically endangered medicinal plant species
title_full Ex situ potential conservation of ipecacuanha (Psychotria ipecacuanha (Brot.) Stokes.), a critically endangered medicinal plant species
title_fullStr Ex situ potential conservation of ipecacuanha (Psychotria ipecacuanha (Brot.) Stokes.), a critically endangered medicinal plant species
title_full_unstemmed Ex situ potential conservation of ipecacuanha (Psychotria ipecacuanha (Brot.) Stokes.), a critically endangered medicinal plant species
title_sort Ex situ potential conservation of ipecacuanha (Psychotria ipecacuanha (Brot.) Stokes.), a critically endangered medicinal plant species
dc.creator.fl_str_mv Urrea Trujillo, Aura Inés
Botero Giraldo, Catalina
Naranjo Gómez, Esther Julia
dc.contributor.author.none.fl_str_mv Urrea Trujillo, Aura Inés
Botero Giraldo, Catalina
Naranjo Gómez, Esther Julia
dc.subject.agrovoc.none.fl_str_mv Vigour
Vigor
In vitro
topic Vigour
Vigor
In vitro
In vitro conservation
Plant limited growth
Osmotic regulator
Recovery phase
http://aims.fao.org/aos/agrovoc/c_37512
http://aims.fao.org/aos/agrovoc/c_37563
dc.subject.proposal.spa.fl_str_mv In vitro conservation
Plant limited growth
Osmotic regulator
Recovery phase
dc.subject.agrovocuri.none.fl_str_mv http://aims.fao.org/aos/agrovoc/c_37512
http://aims.fao.org/aos/agrovoc/c_37563
description ABSTRACT: Psychotria ipecacuanha is a medicinal plant endangered in Colombia, due to the indiscriminate exploitation suffered several decades ago for its therapeutic importance. Its great medicinal value is due to emetine production and its derivatives, mainly in the roots. Given these concerns, the aim of this research was to evaluate different parameters for in vitro conservation of P. ipecacuanha by a limited plant growth, using as in vitro explants plant nodes. Plant growth response to 18, 23 and 25°C with and without addition to MS basal culture medium 15 and 30 gL-1 of mannitol osmotic regulator was evaluated. Registration of plant survival data (%), plant height (cm), plant development of axillary shoots, number of leaves, callus formation and vigour of developed buds and was initially performed at 3 months and then every two months until 12 months. The explants conserved for 9 months in culture medium without mannitol osmotic regulator and temperature of 18°C, showed response after transferring and reculturing in recovery culture medium for 6-8 weeks, this process had not achieved any effect on plant growth and vigour in the recovery phase. In addition, the critical parameters in the acclimatization process were determined.
publishDate 2017
dc.date.issued.none.fl_str_mv 2017
dc.date.accessioned.none.fl_str_mv 2021-06-25T03:22:06Z
dc.date.available.none.fl_str_mv 2021-06-25T03:22:06Z
dc.type.spa.fl_str_mv info:eu-repo/semantics/article
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dc.type.local.spa.fl_str_mv Artículo de investigación
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dc.identifier.citation.spa.fl_str_mv Urrea Trujillo, A. I., Botero Giraldo, C., & Naranjo Gómez, E. J. (2017). Ex situ potential conservation of ipecacuanha (Psychotria ipecacuanha (Brot.) Stokes.), a critically endangered medicinal plant species. Acta Agronómica, 66(4), 598 - 605. https://doi.org/10.15446/acag.v66n4.60080
dc.identifier.issn.none.fl_str_mv 0120-2812
dc.identifier.uri.none.fl_str_mv http://hdl.handle.net/10495/20373
dc.identifier.doi.none.fl_str_mv 10.15446/acag.v66n4.60080
dc.identifier.eissn.none.fl_str_mv 2323-0118
identifier_str_mv Urrea Trujillo, A. I., Botero Giraldo, C., & Naranjo Gómez, E. J. (2017). Ex situ potential conservation of ipecacuanha (Psychotria ipecacuanha (Brot.) Stokes.), a critically endangered medicinal plant species. Acta Agronómica, 66(4), 598 - 605. https://doi.org/10.15446/acag.v66n4.60080
0120-2812
10.15446/acag.v66n4.60080
2323-0118
url http://hdl.handle.net/10495/20373
dc.language.iso.spa.fl_str_mv eng
language eng
dc.relation.ispartofjournalabbrev.spa.fl_str_mv Acta agron.
dc.rights.spa.fl_str_mv info:eu-repo/semantics/openAccess
dc.rights.uri.*.fl_str_mv http://creativecommons.org/licenses/by-nc-nd/2.5/co/
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dc.format.extent.spa.fl_str_mv 8
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dc.publisher.spa.fl_str_mv Universidad Nacional de Colombia
dc.publisher.group.spa.fl_str_mv Biotecnología
dc.publisher.place.spa.fl_str_mv Palmira, Colombia
institution Universidad de Antioquia
bitstream.url.fl_str_mv http://bibliotecadigital.udea.edu.co/bitstream/10495/20373/1/UrreaAura_2017_ConservationIpecacuanha.pdf
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repository.name.fl_str_mv Repositorio Institucional Universidad de Antioquia
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spelling Urrea Trujillo, Aura InésBotero Giraldo, CatalinaNaranjo Gómez, Esther Julia2021-06-25T03:22:06Z2021-06-25T03:22:06Z2017Urrea Trujillo, A. I., Botero Giraldo, C., & Naranjo Gómez, E. J. (2017). Ex situ potential conservation of ipecacuanha (Psychotria ipecacuanha (Brot.) Stokes.), a critically endangered medicinal plant species. Acta Agronómica, 66(4), 598 - 605. https://doi.org/10.15446/acag.v66n4.600800120-2812http://hdl.handle.net/10495/2037310.15446/acag.v66n4.600802323-0118ABSTRACT: Psychotria ipecacuanha is a medicinal plant endangered in Colombia, due to the indiscriminate exploitation suffered several decades ago for its therapeutic importance. Its great medicinal value is due to emetine production and its derivatives, mainly in the roots. Given these concerns, the aim of this research was to evaluate different parameters for in vitro conservation of P. ipecacuanha by a limited plant growth, using as in vitro explants plant nodes. Plant growth response to 18, 23 and 25°C with and without addition to MS basal culture medium 15 and 30 gL-1 of mannitol osmotic regulator was evaluated. Registration of plant survival data (%), plant height (cm), plant development of axillary shoots, number of leaves, callus formation and vigour of developed buds and was initially performed at 3 months and then every two months until 12 months. The explants conserved for 9 months in culture medium without mannitol osmotic regulator and temperature of 18°C, showed response after transferring and reculturing in recovery culture medium for 6-8 weeks, this process had not achieved any effect on plant growth and vigour in the recovery phase. In addition, the critical parameters in the acclimatization process were determined.RESUMEN: Psychotria ipecacuanha es una especie medicinal amenazada en Colombia, debido a la explotación indiscriminada que varias décadas atrás sufrió por su importancia terapéutica. Su gran valor medicinal se debe a la producción de emetina y sus derivados, principalmente en las raíces. Ante este panorama, el presente estudio tuvo como objetivo evaluar diferentes parámetros para la conservación in vitro de P. ipecacuanha por crecimiento limitado, usando como explantes nudos de plantas in vitro. Se evaluó la respuesta de crecimiento a 18, 23 y 25°C sin y con la adición al medio de cultivo basal MS del regulador osmótico manitol a 15 y 30 gL-1. El registro de los datos de supervivencia (%), altura (cm), desarrollo de brotes axilares, número de hojas, formación de callo y vigor de los brotes desarrollados, se realizó inicialmente a los 3 meses y luego cada dos meses hasta completar 12 meses. Una temperatura de 18°C, sin la adición del regulador osmótico manitol al medio cultivo basal MS, permitió mantener vitroplantas de esta especie hasta por nueve meses, sin verse afectado el crecimiento y vigor en la etapa de recuperación. Además se logró determinar los parámetros críticos en el proceso de aclimatización.COL00010848application/pdfengUniversidad Nacional de ColombiaBiotecnologíaPalmira, Colombiainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_2df8fbb1https://purl.org/redcol/resource_type/ARTArtículo de investigaciónhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-nd/2.5/co/http://purl.org/coar/access_right/c_abf2https://creativecommons.org/licenses/by-nc-nd/4.0/Ex situ potential conservation of ipecacuanha (Psychotria ipecacuanha (Brot.) Stokes.), a critically endangered medicinal plant speciesPotencial de conservación ex situ de ipecacuana (Psychotria ipecacuanha (Brot.) Stokes.), una planta medicinal en peligro crítico de extinciónVigourVigorIn vitroIn vitro conservationPlant limited growthOsmotic regulatorRecovery phasehttp://aims.fao.org/aos/agrovoc/c_37512http://aims.fao.org/aos/agrovoc/c_37563Acta agron.Acta Agronómica598605664ORIGINALUrreaAura_2017_ConservationIpecacuanha.pdfUrreaAura_2017_ConservationIpecacuanha.pdfArtículo de investigaciónapplication/pdf33149605http://bibliotecadigital.udea.edu.co/bitstream/10495/20373/1/UrreaAura_2017_ConservationIpecacuanha.pdfdccc331e9743bc794df8b5f1d7dd5f4fMD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748http://bibliotecadigital.udea.edu.co/bitstream/10495/20373/3/license.txt8a4605be74aa9ea9d79846c1fba20a33MD53CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8823http://bibliotecadigital.udea.edu.co/bitstream/10495/20373/2/license_rdfb88b088d9957e670ce3b3fbe2eedbc13MD5210495/20373oai:bibliotecadigital.udea.edu.co:10495/203732021-06-24 22:22:07.134Repositorio Institucional Universidad de Antioquiaandres.perez@udea.edu.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