Antiplasmodial and Cytotoxic Activity of Piper Piedecuestanum Trel. and Yunck

ABSTRACT: Background and Objective: Plasmodium resistance to antimalarial drugs has expanded and intensified, making new and effective antimalarial drugs urgently. The objective of this work was the in vitro evaluation of antiplasmodial activity of extracts of different polarity and compounds of the...

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Autores:
Mesa Vanegas, Ana María
Toro Suaza, Jhon Fredy
Vásquez Cardona, Ana María
Blair Trujillo, Silvia
Peláez Jaramillo, Carlos
Díaz Oltra, Santiago
Pachón, César Augusto
Carda, Miguel
Tipo de recurso:
Article of investigation
Fecha de publicación:
2018
Institución:
Universidad de Antioquia
Repositorio:
Repositorio UdeA
Idioma:
eng
OAI Identifier:
oai:bibliotecadigital.udea.edu.co:10495/29711
Acceso en línea:
https://hdl.handle.net/10495/29711
Palabra clave:
Plasmodium falciparum
Piper pidecuestanum
Antiplasmodial activity
Cytotoxic activity
Rights
openAccess
License
http://creativecommons.org/licenses/by-sa/2.5/co/
Description
Summary:ABSTRACT: Background and Objective: Plasmodium resistance to antimalarial drugs has expanded and intensified, making new and effective antimalarial drugs urgently. The objective of this work was the in vitro evaluation of antiplasmodial activity of extracts of different polarity and compounds of the species P. piedecuestanum. Materials and Methods: The plant materials were obtained through successive extractions using solvents of different polarity such as hexane (H), dichloromethane (D), ethyl acetate (A) and methanol (M) and separations techniques for fractionation and isolation of compounds. The antiplasmodial activities of the extracts and compounds were evaluated by SYBR Green I® method and evaluated the cytotoxicity in the cell lines U-937, HUVEC by the MTT method. Results: The antiplasmodial and cytotoxic activity of the extracts of dichloromethane (PPD) and ethyl acetate (PPAE) with antiplasmodial activity of IC50 = 17.93 µg mLG1 ; IS = 2.093 and IC50 = 19.5 µg mLG1 ; IS = 0.791, respectively are reported for the first time. In addition, from P. piedecuestanum species were isolation and characterization five metabolites 5,8-Hydroxy-7-methoxyflavone(1), 6,7-dimethoxy-5,8- dihydroxyflavone(2), 6,7-dimethoxy-5-hydroxyflavone (mosloflavone) (3), 5,6-dihydroxy-7-methoxyflavone (negletein) (4), 5-hydroxy-7- methoxyflavone (5) and a brominated derivative from (5) named 6,8 bromo-5-hydroxy-7-methoxyflavone(7). Compound (1) presented promising antiplasmodial activity with an IC50 = 7.325 µg mLG1 (25.69 µM); ISHUVEC =13.65. Conclusion: Chemical analysis of extracts and compounds from P. piedecuestanum spices will play a central role in the development and modernization of an antimalarial herbal traditional in Colombia.