Enzymatic transformation of crystalline structure of copolymer poly (ethylene-co-vinyl alcohol) (evoh)

ABSTRACT: Changes in the crystalline structure of the copolymer poly(ethylene-co-vinyl alcohol) (EVOH) that occurs during the enzymatic fermentation system with the fungus Phanerochaete chrysosoporium were studied. This fungus has a powerful group of oxidative enzymes that had been used for several...

Full description

Autores:
Arboleda Echavarría, Carolina
Segura Sánchez, Freimar
Mejía Gallón, Amanda Inés
Tipo de recurso:
Article of investigation
Fecha de publicación:
2007
Institución:
Universidad de Antioquia
Repositorio:
Repositorio UdeA
Idioma:
eng
OAI Identifier:
oai:bibliotecadigital.udea.edu.co:10495/20900
Acceso en línea:
http://hdl.handle.net/10495/20900
https://revistas.udea.edu.co/index.php/vitae/article/view/565
Palabra clave:
Copolímeros
Copolymers
Polietilvinialcohol
Ligninoperoxidasa
Phanerochaete chrysosporium
Rights
openAccess
License
http://creativecommons.org/licenses/by-nc-sa/2.5/co/
id UDEA2_5cc9968667e9078ad8731389483e9427
oai_identifier_str oai:bibliotecadigital.udea.edu.co:10495/20900
network_acronym_str UDEA2
network_name_str Repositorio UdeA
repository_id_str
dc.title.spa.fl_str_mv Enzymatic transformation of crystalline structure of copolymer poly (ethylene-co-vinyl alcohol) (evoh)
dc.title.alternative.spa.fl_str_mv Transformación enzimática de la estructura cristalina del copolímero polietil-vinil alcohol (evoh)
title Enzymatic transformation of crystalline structure of copolymer poly (ethylene-co-vinyl alcohol) (evoh)
spellingShingle Enzymatic transformation of crystalline structure of copolymer poly (ethylene-co-vinyl alcohol) (evoh)
Copolímeros
Copolymers
Polietilvinialcohol
Ligninoperoxidasa
Phanerochaete chrysosporium
title_short Enzymatic transformation of crystalline structure of copolymer poly (ethylene-co-vinyl alcohol) (evoh)
title_full Enzymatic transformation of crystalline structure of copolymer poly (ethylene-co-vinyl alcohol) (evoh)
title_fullStr Enzymatic transformation of crystalline structure of copolymer poly (ethylene-co-vinyl alcohol) (evoh)
title_full_unstemmed Enzymatic transformation of crystalline structure of copolymer poly (ethylene-co-vinyl alcohol) (evoh)
title_sort Enzymatic transformation of crystalline structure of copolymer poly (ethylene-co-vinyl alcohol) (evoh)
dc.creator.fl_str_mv Arboleda Echavarría, Carolina
Segura Sánchez, Freimar
Mejía Gallón, Amanda Inés
dc.contributor.author.none.fl_str_mv Arboleda Echavarría, Carolina
Segura Sánchez, Freimar
Mejía Gallón, Amanda Inés
dc.subject.lemb.none.fl_str_mv Copolímeros
Copolymers
topic Copolímeros
Copolymers
Polietilvinialcohol
Ligninoperoxidasa
Phanerochaete chrysosporium
dc.subject.proposal.spa.fl_str_mv Polietilvinialcohol
Ligninoperoxidasa
Phanerochaete chrysosporium
description ABSTRACT: Changes in the crystalline structure of the copolymer poly(ethylene-co-vinyl alcohol) (EVOH) that occurs during the enzymatic fermentation system with the fungus Phanerochaete chrysosoporium were studied. This fungus has a powerful group of oxidative enzymes that had been used for several processes of environmental pollutants degradation. In order to use the enzymatic system of the fungus to improve degradation, films of the copolymer are inoculated and maintained in a solid state fermentation (SSF) system using as substrate a mixture of agroindustrial corn wastes with an initial humidity of 90%, during forty days. Analyses of the films were made at different times of fermentation. The effect on the chemical structure of the polymer was studied by using FTIR-spectroscopy with attenuated total reflection (ATR) and changes in the crystalline structure were determined by differential scan calorimetry DSC and X-ray diffraction technique. The lignin peroxidase (LiP) activity was followed by UV-spectrophotometry. We concluded that after 20 days of fermentation the copolymer has lost 50 percent of the initial crystalline structure.
publishDate 2007
dc.date.issued.none.fl_str_mv 2007
dc.date.accessioned.none.fl_str_mv 2021-07-15T20:23:59Z
dc.date.available.none.fl_str_mv 2021-07-15T20:23:59Z
dc.type.spa.fl_str_mv info:eu-repo/semantics/article
dc.type.coarversion.fl_str_mv http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.hasversion.spa.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.coar.spa.fl_str_mv http://purl.org/coar/resource_type/c_2df8fbb1
dc.type.redcol.spa.fl_str_mv https://purl.org/redcol/resource_type/ART
dc.type.local.spa.fl_str_mv Artículo de investigación
format http://purl.org/coar/resource_type/c_2df8fbb1
status_str publishedVersion
dc.identifier.issn.none.fl_str_mv 0121-4004
dc.identifier.uri.none.fl_str_mv http://hdl.handle.net/10495/20900
dc.identifier.eissn.none.fl_str_mv 2145-2660
dc.identifier.url.spa.fl_str_mv https://revistas.udea.edu.co/index.php/vitae/article/view/565
identifier_str_mv 0121-4004
2145-2660
url http://hdl.handle.net/10495/20900
https://revistas.udea.edu.co/index.php/vitae/article/view/565
dc.language.iso.spa.fl_str_mv eng
language eng
dc.relation.ispartofjournalabbrev.spa.fl_str_mv Vitae
dc.rights.spa.fl_str_mv info:eu-repo/semantics/openAccess
dc.rights.uri.*.fl_str_mv http://creativecommons.org/licenses/by-nc-sa/2.5/co/
dc.rights.accessrights.spa.fl_str_mv http://purl.org/coar/access_right/c_abf2
dc.rights.creativecommons.spa.fl_str_mv https://creativecommons.org/licenses/by-nc-sa/4.0/
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/2.5/co/
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by-nc-sa/4.0/
dc.format.extent.spa.fl_str_mv 6
dc.format.mimetype.spa.fl_str_mv application/pdf
dc.publisher.spa.fl_str_mv Universidad de Antioquia, Facultad de Ciencias Farmacéuticas y Alimentarias
dc.publisher.group.spa.fl_str_mv BIOPOLIMER
Ciencia de los Materiales
dc.publisher.place.spa.fl_str_mv Medellín, Colombia
institution Universidad de Antioquia
bitstream.url.fl_str_mv http://bibliotecadigital.udea.edu.co/bitstream/10495/20900/2/license_rdf
http://bibliotecadigital.udea.edu.co/bitstream/10495/20900/3/license.txt
http://bibliotecadigital.udea.edu.co/bitstream/10495/20900/1/ArboledaCarolina_2007_EnzymaticTransformationStructureCopolymer.pdf
bitstream.checksum.fl_str_mv e2060682c9c70d4d30c83c51448f4eed
8a4605be74aa9ea9d79846c1fba20a33
09a8658d84db1152fd3425cdc8f72c11
bitstream.checksumAlgorithm.fl_str_mv MD5
MD5
MD5
repository.name.fl_str_mv Repositorio Institucional Universidad de Antioquia
repository.mail.fl_str_mv andres.perez@udea.edu.co
_version_ 1812173076890124288
spelling Arboleda Echavarría, CarolinaSegura Sánchez, FreimarMejía Gallón, Amanda Inés2021-07-15T20:23:59Z2021-07-15T20:23:59Z20070121-4004http://hdl.handle.net/10495/209002145-2660https://revistas.udea.edu.co/index.php/vitae/article/view/565ABSTRACT: Changes in the crystalline structure of the copolymer poly(ethylene-co-vinyl alcohol) (EVOH) that occurs during the enzymatic fermentation system with the fungus Phanerochaete chrysosoporium were studied. This fungus has a powerful group of oxidative enzymes that had been used for several processes of environmental pollutants degradation. In order to use the enzymatic system of the fungus to improve degradation, films of the copolymer are inoculated and maintained in a solid state fermentation (SSF) system using as substrate a mixture of agroindustrial corn wastes with an initial humidity of 90%, during forty days. Analyses of the films were made at different times of fermentation. The effect on the chemical structure of the polymer was studied by using FTIR-spectroscopy with attenuated total reflection (ATR) and changes in the crystalline structure were determined by differential scan calorimetry DSC and X-ray diffraction technique. The lignin peroxidase (LiP) activity was followed by UV-spectrophotometry. We concluded that after 20 days of fermentation the copolymer has lost 50 percent of the initial crystalline structure.RESUMEN: Se estudia el cambio en la estructura cristalina del copolímero polietilvinil alcohol (EVOH) que tiene lugar durante el sistema de fermentación enzimática con el hongo Phanerochaete chrysosporium. El hongo cuenta con un poderoso grupo de enzimas oxidativa, que han sido utilizadas en la degradación de varios contaminantes ambientales. Con el fin de utilizar el sistema enzimático de este microorganismo en la degradación del EVOH, las películas del copolímero fueron inoculadas y mantenidas bajo un sistema de fermentación en estado sólido, utilizando como sustrato la mezcla de residuos agroindustriales de maíz, con una humedad inicial del 90%, durante 40 días. El análisis de las películas se llevó a cabo en diferentes tiempos de fermentación; los cambios en la estructura cristalina fueron determinados por calorimetría diferencial de barrido DSC y difracción de rayos X. La actividad de la enzima ligninoperoxidasa fue seguida por espectrofotometría ultravioleta. Concluimos que después de 20 días de fermentación, el copolímero pierde el 50% de la cristalinidad inicial.COL0065152COL00024016application/pdfengUniversidad de Antioquia, Facultad de Ciencias Farmacéuticas y AlimentariasBIOPOLIMERCiencia de los MaterialesMedellín, Colombiainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_2df8fbb1https://purl.org/redcol/resource_type/ARTArtículo de investigaciónhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/2.5/co/http://purl.org/coar/access_right/c_abf2https://creativecommons.org/licenses/by-nc-sa/4.0/Enzymatic transformation of crystalline structure of copolymer poly (ethylene-co-vinyl alcohol) (evoh)Transformación enzimática de la estructura cristalina del copolímero polietil-vinil alcohol (evoh)CopolímerosCopolymersPolietilvinialcoholLigninoperoxidasaPhanerochaete chrysosporiumVitaeVitae2530141CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-81051http://bibliotecadigital.udea.edu.co/bitstream/10495/20900/2/license_rdfe2060682c9c70d4d30c83c51448f4eedMD52LICENSElicense.txtlicense.txttext/plain; charset=utf-81748http://bibliotecadigital.udea.edu.co/bitstream/10495/20900/3/license.txt8a4605be74aa9ea9d79846c1fba20a33MD53ORIGINALArboledaCarolina_2007_EnzymaticTransformationStructureCopolymer.pdfArboledaCarolina_2007_EnzymaticTransformationStructureCopolymer.pdfArtículo de investigaciónapplication/pdf184512http://bibliotecadigital.udea.edu.co/bitstream/10495/20900/1/ArboledaCarolina_2007_EnzymaticTransformationStructureCopolymer.pdf09a8658d84db1152fd3425cdc8f72c11MD5110495/20900oai:bibliotecadigital.udea.edu.co:10495/209002021-07-30 18:18:05.773Repositorio Institucional Universidad de Antioquiaandres.perez@udea.edu.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