Genetic diversity of Senepol cattle in Colombia using ten multiplexed microsatellites
ABSTRACT: An automated multiplex PCR assay of 10 polymorphic microsatellite loci was optimized here in Senepol bovine cattle. Allele frequencies were estimated for Colombian herds. The power of this set of markers for parentage and kinship analyses was also tested. Population genetic parameters, com...
- Autores:
-
Londoño Díaz, Natalia
Cerón Muñoz, Mario Fernando
Soto Calderón, Iván Darío
- Tipo de recurso:
- Article of investigation
- Fecha de publicación:
- 2016
- Institución:
- Universidad de Antioquia
- Repositorio:
- Repositorio UdeA
- Idioma:
- eng
- OAI Identifier:
- oai:bibliotecadigital.udea.edu.co:10495/34422
- Acceso en línea:
- https://hdl.handle.net/10495/34422
- Palabra clave:
- Paternidad
Paternity
Bos taurus
Marcadores genéticos
Genetic markers
Ascendencia
Ancestry
Variación genética
Genetic variation
http://aims.fao.org/aos/agrovoc/c_9372
http://aims.fao.org/aos/agrovoc/c_24030
http://aims.fao.org/aos/agrovoc/c_394
http://aims.fao.org/aos/agrovoc/c_15975
- Rights
- openAccess
- License
- http://creativecommons.org/licenses/by-nc-sa/2.5/co/
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| dc.title.spa.fl_str_mv |
Genetic diversity of Senepol cattle in Colombia using ten multiplexed microsatellites |
| dc.title.translated.spa.fl_str_mv |
Diversidad genética de ganado Senepol en Colombia empleando diez microsatélites combinados |
| title |
Genetic diversity of Senepol cattle in Colombia using ten multiplexed microsatellites |
| spellingShingle |
Genetic diversity of Senepol cattle in Colombia using ten multiplexed microsatellites Paternidad Paternity Bos taurus Marcadores genéticos Genetic markers Ascendencia Ancestry Variación genética Genetic variation http://aims.fao.org/aos/agrovoc/c_9372 http://aims.fao.org/aos/agrovoc/c_24030 http://aims.fao.org/aos/agrovoc/c_394 http://aims.fao.org/aos/agrovoc/c_15975 |
| title_short |
Genetic diversity of Senepol cattle in Colombia using ten multiplexed microsatellites |
| title_full |
Genetic diversity of Senepol cattle in Colombia using ten multiplexed microsatellites |
| title_fullStr |
Genetic diversity of Senepol cattle in Colombia using ten multiplexed microsatellites |
| title_full_unstemmed |
Genetic diversity of Senepol cattle in Colombia using ten multiplexed microsatellites |
| title_sort |
Genetic diversity of Senepol cattle in Colombia using ten multiplexed microsatellites |
| dc.creator.fl_str_mv |
Londoño Díaz, Natalia Cerón Muñoz, Mario Fernando Soto Calderón, Iván Darío |
| dc.contributor.author.none.fl_str_mv |
Londoño Díaz, Natalia Cerón Muñoz, Mario Fernando Soto Calderón, Iván Darío |
| dc.contributor.researchgroup.spa.fl_str_mv |
Grupo de Investigación en Agrociencias Biodiversidad y Territorio GAMMA |
| dc.subject.decs.none.fl_str_mv |
Paternidad Paternity |
| topic |
Paternidad Paternity Bos taurus Marcadores genéticos Genetic markers Ascendencia Ancestry Variación genética Genetic variation http://aims.fao.org/aos/agrovoc/c_9372 http://aims.fao.org/aos/agrovoc/c_24030 http://aims.fao.org/aos/agrovoc/c_394 http://aims.fao.org/aos/agrovoc/c_15975 |
| dc.subject.agrovoc.none.fl_str_mv |
Bos taurus Marcadores genéticos Genetic markers Ascendencia Ancestry Variación genética Genetic variation |
| dc.subject.agrovocuri.none.fl_str_mv |
http://aims.fao.org/aos/agrovoc/c_9372 http://aims.fao.org/aos/agrovoc/c_24030 http://aims.fao.org/aos/agrovoc/c_394 http://aims.fao.org/aos/agrovoc/c_15975 |
| description |
ABSTRACT: An automated multiplex PCR assay of 10 polymorphic microsatellite loci was optimized here in Senepol bovine cattle. Allele frequencies were estimated for Colombian herds. The power of this set of markers for parentage and kinship analyses was also tested. Population genetic parameters, combined probability of identity (CPI) and combined probabilities of exclusion (CPE) were estimated. The average number of alleles per locus was 14, the effective number of alleles was 5, the observed and expected heterozygosities were 0.635 and 0.776, respectively, the Shannon Index was 2.70 and the polymorphic information content was 0.751. These values evidenced elevated levels of polymorphism and an underestimation of genetic diversity through the use of manual genotyping methods. Inbreeding coefficient (Fis=0.206) in Colombian Senepol herds was moderate. Altogether, data from the used set of microsatellite loci yielded a CPI between two random samples of 1.07E-12. The CPE in parentage testing were >0.998 for the first parent and >0.999 for both the second parent and parent pairs. This set of polymorphic markers can be genotyped in a relatively easy, effective and reliable way for relatedness and forensic analyses. Finally, we recommend our approach for population genetic analyses of other Senepol populations and future genetic monitoring of inbreeding in Senepol herds. |
| publishDate |
2016 |
| dc.date.issued.none.fl_str_mv |
2016 |
| dc.date.accessioned.none.fl_str_mv |
2023-04-01T21:29:55Z |
| dc.date.available.none.fl_str_mv |
2023-04-01T21:29:55Z |
| dc.type.spa.fl_str_mv |
Artículo de investigación |
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http://purl.org/coar/resource_type/c_2df8fbb1 |
| dc.type.redcol.spa.fl_str_mv |
https://purl.org/redcol/resource_type/ART |
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http://purl.org/coar/version/c_970fb48d4fbd8a85 |
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info:eu-repo/semantics/article |
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info:eu-repo/semantics/publishedVersion |
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http://purl.org/coar/resource_type/c_2df8fbb1 |
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publishedVersion |
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0121-3784 |
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https://hdl.handle.net/10495/34422 |
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0121-3784 |
| url |
https://hdl.handle.net/10495/34422 |
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eng |
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eng |
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LRRD |
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7 |
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8 |
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1 |
| dc.relation.citationvolume.spa.fl_str_mv |
28 |
| dc.relation.ispartofjournal.spa.fl_str_mv |
Livestock Research for Rural Development |
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http://creativecommons.org/licenses/by-nc-sa/2.5/co/ |
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https://creativecommons.org/licenses/by-nc-sa/4.0/ |
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application/pdf |
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Centro para la Investigación en Sistemas Sostenibles de Producción Agropecuaria |
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Cali, Colombia |
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sin facultad - programa |
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Universidad de Antioquia |
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Londoño Díaz, NataliaCerón Muñoz, Mario FernandoSoto Calderón, Iván DaríoGrupo de Investigación en Agrociencias Biodiversidad y Territorio GAMMA2023-04-01T21:29:55Z2023-04-01T21:29:55Z20160121-3784https://hdl.handle.net/10495/34422ABSTRACT: An automated multiplex PCR assay of 10 polymorphic microsatellite loci was optimized here in Senepol bovine cattle. Allele frequencies were estimated for Colombian herds. The power of this set of markers for parentage and kinship analyses was also tested. Population genetic parameters, combined probability of identity (CPI) and combined probabilities of exclusion (CPE) were estimated. The average number of alleles per locus was 14, the effective number of alleles was 5, the observed and expected heterozygosities were 0.635 and 0.776, respectively, the Shannon Index was 2.70 and the polymorphic information content was 0.751. These values evidenced elevated levels of polymorphism and an underestimation of genetic diversity through the use of manual genotyping methods. Inbreeding coefficient (Fis=0.206) in Colombian Senepol herds was moderate. Altogether, data from the used set of microsatellite loci yielded a CPI between two random samples of 1.07E-12. The CPE in parentage testing were >0.998 for the first parent and >0.999 for both the second parent and parent pairs. This set of polymorphic markers can be genotyped in a relatively easy, effective and reliable way for relatedness and forensic analyses. Finally, we recommend our approach for population genetic analyses of other Senepol populations and future genetic monitoring of inbreeding in Senepol herds.RESUMEN: Se optimizó en ganado bovino Senepol una prueba automatizada de PCR multiplex de 10 loci microsatélite polimórficos. Adicionalmente, se estimaron las frecuencias alélicas en hatos colombianos y se evaluó el poder de este grupo de marcadores en pruebas de paternidad y parentesco. Se estimaron parámetros genético-poblacionales, probabilidad de identidad combinada (CPI) y probabilidades de exclusión combinadas (CPE). El número promedio de alelos por locus fue 14, el número efectivo de alelos fue 5, las heterocigocidades observada y esperada fueron 0.635 y 0.776, respectivamente, el índice de Shannon fue 2. 70 y el contenido de información polimórfica fue 0.751. Estos valores evidenciaron altos niveles de polimorfismo y una subestimación de la diversidad genética cuando se usan métodos manuales de genotipificación. El coeficiente de endogamia en hatos colombianos de Senepol fue moderado (Fis=0.206). En general, los datos del grupo utilizado de microsatélites arrojaron un CPI entre dos muestras aleatorias de 1.07E-12. El CPE in las pruebas de parentesco fue >0.998 para el primer parental y >0.999 tanto para el segundo parental como para el par de parentales. Este grupo de marcadores microsatélite es relativamente fácil de genotipificar en una forma efectiva y confiable para análisis forenses y de parentesco. Finalmente, recomendamos nuestra aproximación para análisis genéticos-poblacionales de otras poblaciones de Senepol y futuros monitoreos genéticos de endogamia en hatos de Senepol.Universidad de Antioquia. Vicerrectoría de investigación. Comité para el Desarrollo de la Investigación - CODIColombian Association of Senepol Cattle Breeder (ASOSENEPOL)COL000677910application/pdfengCentro para la Investigación en Sistemas Sostenibles de Producción AgropecuariaCali, Colombiasin facultad - programahttp://creativecommons.org/licenses/by-nc-sa/2.5/co/https://creativecommons.org/licenses/by-nc-sa/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Genetic diversity of Senepol cattle in Colombia using ten multiplexed microsatellitesDiversidad genética de ganado Senepol en Colombia empleando diez microsatélites combinadosArtículo de investigaciónhttp://purl.org/coar/resource_type/c_2df8fbb1https://purl.org/redcol/resource_type/ARThttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionPaternidadPaternityBos taurusMarcadores genéticosGenetic markersAscendenciaAncestryVariación genéticaGenetic variationhttp://aims.fao.org/aos/agrovoc/c_9372http://aims.fao.org/aos/agrovoc/c_24030http://aims.fao.org/aos/agrovoc/c_394http://aims.fao.org/aos/agrovoc/c_15975LRRD78128Livestock Research for Rural Developmentgrid.412881.6PublicationORIGINALLondoñoNatalia_2016_Genetic-Diversity-Senepol.pdfLondoñoNatalia_2016_Genetic-Diversity-Senepol.pdfArtículo de investigaciónapplication/pdf267028https://bibliotecadigital.udea.edu.co/bitstreams/79f241b4-2b4b-4906-b2ae-01121d423d5b/downloada35b1150caabc5e1955662c2b37fbf83MD51trueAnonymousREADCC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; 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