Interleukin-4, interleukin-5, and interleukin-13 gene expression in cultured mononuclear cells from porcine circovirus type 2–vaccinated pigs after cells were challenged with porcine circovirus type 2 open reading frame 2 antigen
RESUMEN: Objective—To characterize the kinetics of interleukin (IL)-4, IL-5, and IL-13 secretion in peripheral blood and lymph node mononuclear cells isolated from porcine circovirus type 2 (PCV2)–vaccinated pigs after cells were challenged with PCV2 open reading frame 2 antigen. Animals—10 pigs. Pr...
- Autores:
-
Quereda, Juan J.
Ramis, Guillermo
Pallares, Francisco J.
Chapat, Ludivine
Joisel, Francois
Charreyre, Catherine
Villar Argaiz, David
Goubier, Anne
Muñoz, Antonio
- Tipo de recurso:
- Article of investigation
- Fecha de publicación:
- 2013
- Institución:
- Universidad de Antioquia
- Repositorio:
- Repositorio UdeA
- Idioma:
- eng
- OAI Identifier:
- oai:bibliotecadigital.udea.edu.co:10495/32602
- Acceso en línea:
- https://hdl.handle.net/10495/32602
- Palabra clave:
- Antígenos Virales
Antigens, Viral
Células Cultivadas
Cells, Cultured
Interleucina-13
Interleukin-13
Interleucina-4
Interleukin-4
Interleukin-5
Interleukin-5
Leucocitos Mononucleares
Leukocytes, Mononuclear
Síndrome Multisistémico de Emaciación Posdestete Porcino
Porcine Postweaning Multisystemic Wasting Syndrome
Porcinos
Swine
Enfermedades de los Porcinos
Swine Diseases
- Rights
- openAccess
- License
- http://creativecommons.org/licenses/by-nc-sa/2.5/co/
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|
dc.title.spa.fl_str_mv |
Interleukin-4, interleukin-5, and interleukin-13 gene expression in cultured mononuclear cells from porcine circovirus type 2–vaccinated pigs after cells were challenged with porcine circovirus type 2 open reading frame 2 antigen |
title |
Interleukin-4, interleukin-5, and interleukin-13 gene expression in cultured mononuclear cells from porcine circovirus type 2–vaccinated pigs after cells were challenged with porcine circovirus type 2 open reading frame 2 antigen |
spellingShingle |
Interleukin-4, interleukin-5, and interleukin-13 gene expression in cultured mononuclear cells from porcine circovirus type 2–vaccinated pigs after cells were challenged with porcine circovirus type 2 open reading frame 2 antigen Antígenos Virales Antigens, Viral Células Cultivadas Cells, Cultured Interleucina-13 Interleukin-13 Interleucina-4 Interleukin-4 Interleukin-5 Interleukin-5 Leucocitos Mononucleares Leukocytes, Mononuclear Síndrome Multisistémico de Emaciación Posdestete Porcino Porcine Postweaning Multisystemic Wasting Syndrome Porcinos Swine Enfermedades de los Porcinos Swine Diseases |
title_short |
Interleukin-4, interleukin-5, and interleukin-13 gene expression in cultured mononuclear cells from porcine circovirus type 2–vaccinated pigs after cells were challenged with porcine circovirus type 2 open reading frame 2 antigen |
title_full |
Interleukin-4, interleukin-5, and interleukin-13 gene expression in cultured mononuclear cells from porcine circovirus type 2–vaccinated pigs after cells were challenged with porcine circovirus type 2 open reading frame 2 antigen |
title_fullStr |
Interleukin-4, interleukin-5, and interleukin-13 gene expression in cultured mononuclear cells from porcine circovirus type 2–vaccinated pigs after cells were challenged with porcine circovirus type 2 open reading frame 2 antigen |
title_full_unstemmed |
Interleukin-4, interleukin-5, and interleukin-13 gene expression in cultured mononuclear cells from porcine circovirus type 2–vaccinated pigs after cells were challenged with porcine circovirus type 2 open reading frame 2 antigen |
title_sort |
Interleukin-4, interleukin-5, and interleukin-13 gene expression in cultured mononuclear cells from porcine circovirus type 2–vaccinated pigs after cells were challenged with porcine circovirus type 2 open reading frame 2 antigen |
dc.creator.fl_str_mv |
Quereda, Juan J. Ramis, Guillermo Pallares, Francisco J. Chapat, Ludivine Joisel, Francois Charreyre, Catherine Villar Argaiz, David Goubier, Anne Muñoz, Antonio |
dc.contributor.author.none.fl_str_mv |
Quereda, Juan J. Ramis, Guillermo Pallares, Francisco J. Chapat, Ludivine Joisel, Francois Charreyre, Catherine Villar Argaiz, David Goubier, Anne Muñoz, Antonio |
dc.subject.decs.none.fl_str_mv |
Antígenos Virales Antigens, Viral Células Cultivadas Cells, Cultured Interleucina-13 Interleukin-13 Interleucina-4 Interleukin-4 Interleukin-5 Interleukin-5 Leucocitos Mononucleares Leukocytes, Mononuclear Síndrome Multisistémico de Emaciación Posdestete Porcino Porcine Postweaning Multisystemic Wasting Syndrome Porcinos Swine Enfermedades de los Porcinos Swine Diseases |
topic |
Antígenos Virales Antigens, Viral Células Cultivadas Cells, Cultured Interleucina-13 Interleukin-13 Interleucina-4 Interleukin-4 Interleukin-5 Interleukin-5 Leucocitos Mononucleares Leukocytes, Mononuclear Síndrome Multisistémico de Emaciación Posdestete Porcino Porcine Postweaning Multisystemic Wasting Syndrome Porcinos Swine Enfermedades de los Porcinos Swine Diseases |
description |
RESUMEN: Objective—To characterize the kinetics of interleukin (IL)-4, IL-5, and IL-13 secretion in peripheral blood and lymph node mononuclear cells isolated from porcine circovirus type 2 (PCV2)–vaccinated pigs after cells were challenged with PCV2 open reading frame 2 antigen. Animals—10 pigs. Procedures—5 pigs were vaccinated with a PCV2 vaccine and received a booster dose 3 weeks later. They were kept together with a similar group of 5 nonvaccinated pigs that served as controls. One week after the second vaccination, peripheral blood mononuclear cells (PBMCs) and excised retropharyngeal lymph node mononuclear cells (LNMCs) were isolated and cultured. Cells were then challenged by exposure to PCV2 open reading frame 2 and evaluated at 2, 12, 24, and 48 hours to determine the expression of IL-4, IL-5, and IL-13 via quantitative PCR assay. Changes in gene expression were analyzed relative to the results from analysis of the sample at 0 hours (calibrator). Results—All ILs were upregulated differently in LNMCs and PBMCs from vaccinated pigs. Lymph node mononuclear cells from vaccinated animals produced significantly more IL-4 mRNA than did PBMCs at 2, 12, and 48 hours (relative change: 2.8 vs −3.6, 13.0 vs 3.6, and 9.8 vs 1.8, respectively) and more IL-5 mRNA at 2, 12, 24, and 48 hours (relative change: 1. 2 vs −4.8, 2.2 vs 0.2, 3.2 vs −1.9, and 4.0 vs −3.6, respectively). Interleukin-13 mRNA reached its highest concentration at 24 hours but was 11.9-fold higher in PBMCs than in LNMCs. |
publishDate |
2013 |
dc.date.issued.none.fl_str_mv |
2013 |
dc.date.accessioned.none.fl_str_mv |
2022-12-06T16:37:26Z |
dc.date.available.none.fl_str_mv |
2022-12-06T16:37:26Z |
dc.type.spa.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.coarversion.fl_str_mv |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
dc.type.hasversion.spa.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.coar.spa.fl_str_mv |
http://purl.org/coar/resource_type/c_2df8fbb1 |
dc.type.redcol.spa.fl_str_mv |
https://purl.org/redcol/resource_type/ART |
dc.type.local.spa.fl_str_mv |
Artículo de investigación |
format |
http://purl.org/coar/resource_type/c_2df8fbb1 |
status_str |
publishedVersion |
dc.identifier.issn.none.fl_str_mv |
0002-9645 |
dc.identifier.uri.none.fl_str_mv |
https://hdl.handle.net/10495/32602 |
dc.identifier.eissn.none.fl_str_mv |
1943-5681 |
identifier_str_mv |
0002-9645 1943-5681 |
url |
https://hdl.handle.net/10495/32602 |
dc.language.iso.spa.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartofjournalabbrev.spa.fl_str_mv |
Am. J. Vet. Res. |
dc.rights.spa.fl_str_mv |
info:eu-repo/semantics/openAccess |
dc.rights.uri.*.fl_str_mv |
http://creativecommons.org/licenses/by-nc-sa/2.5/co/ |
dc.rights.accessrights.spa.fl_str_mv |
http://purl.org/coar/access_right/c_abf2 |
dc.rights.creativecommons.spa.fl_str_mv |
https://creativecommons.org/licenses/by-nc/4.0/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by-nc-sa/2.5/co/ http://purl.org/coar/access_right/c_abf2 https://creativecommons.org/licenses/by-nc/4.0/ |
dc.format.extent.spa.fl_str_mv |
4 |
dc.format.mimetype.spa.fl_str_mv |
application/pdf |
dc.publisher.spa.fl_str_mv |
American Veterinary Medical Association |
dc.publisher.group.spa.fl_str_mv |
Grupo Vericel |
dc.publisher.place.spa.fl_str_mv |
Chicago, Estados Unidos |
institution |
Universidad de Antioquia |
bitstream.url.fl_str_mv |
https://bibliotecadigital.udea.edu.co/bitstream/10495/32602/2/license_rdf https://bibliotecadigital.udea.edu.co/bitstream/10495/32602/1/VillarDavid_2013_Interleukin-4.pdf https://bibliotecadigital.udea.edu.co/bitstream/10495/32602/3/license.txt |
bitstream.checksum.fl_str_mv |
e2060682c9c70d4d30c83c51448f4eed de04040fb2ae33b56450eef3f29bd927 8a4605be74aa9ea9d79846c1fba20a33 |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 MD5 |
repository.name.fl_str_mv |
Repositorio Institucional Universidad de Antioquia |
repository.mail.fl_str_mv |
andres.perez@udea.edu.co |
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1812173094438043648 |
spelling |
Quereda, Juan J.Ramis, GuillermoPallares, Francisco J.Chapat, LudivineJoisel, FrancoisCharreyre, CatherineVillar Argaiz, DavidGoubier, AnneMuñoz, Antonio2022-12-06T16:37:26Z2022-12-06T16:37:26Z20130002-9645https://hdl.handle.net/10495/326021943-5681RESUMEN: Objective—To characterize the kinetics of interleukin (IL)-4, IL-5, and IL-13 secretion in peripheral blood and lymph node mononuclear cells isolated from porcine circovirus type 2 (PCV2)–vaccinated pigs after cells were challenged with PCV2 open reading frame 2 antigen. Animals—10 pigs. Procedures—5 pigs were vaccinated with a PCV2 vaccine and received a booster dose 3 weeks later. They were kept together with a similar group of 5 nonvaccinated pigs that served as controls. One week after the second vaccination, peripheral blood mononuclear cells (PBMCs) and excised retropharyngeal lymph node mononuclear cells (LNMCs) were isolated and cultured. Cells were then challenged by exposure to PCV2 open reading frame 2 and evaluated at 2, 12, 24, and 48 hours to determine the expression of IL-4, IL-5, and IL-13 via quantitative PCR assay. Changes in gene expression were analyzed relative to the results from analysis of the sample at 0 hours (calibrator). Results—All ILs were upregulated differently in LNMCs and PBMCs from vaccinated pigs. Lymph node mononuclear cells from vaccinated animals produced significantly more IL-4 mRNA than did PBMCs at 2, 12, and 48 hours (relative change: 2.8 vs −3.6, 13.0 vs 3.6, and 9.8 vs 1.8, respectively) and more IL-5 mRNA at 2, 12, 24, and 48 hours (relative change: 1. 2 vs −4.8, 2.2 vs 0.2, 3.2 vs −1.9, and 4.0 vs −3.6, respectively). Interleukin-13 mRNA reached its highest concentration at 24 hours but was 11.9-fold higher in PBMCs than in LNMCs.COL00780894application/pdfengAmerican Veterinary Medical AssociationGrupo VericelChicago, Estados Unidosinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_2df8fbb1https://purl.org/redcol/resource_type/ARTArtículo de investigaciónhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/2.5/co/http://purl.org/coar/access_right/c_abf2https://creativecommons.org/licenses/by-nc/4.0/Interleukin-4, interleukin-5, and interleukin-13 gene expression in cultured mononuclear cells from porcine circovirus type 2–vaccinated pigs after cells were challenged with porcine circovirus type 2 open reading frame 2 antigenAntígenos ViralesAntigens, ViralCélulas CultivadasCells, CulturedInterleucina-13Interleukin-13Interleucina-4Interleukin-4Interleukin-5Interleukin-5Leucocitos MononuclearesLeukocytes, MononuclearSíndrome Multisistémico de Emaciación Posdestete PorcinoPorcine Postweaning Multisystemic Wasting SyndromePorcinosSwineEnfermedades de los PorcinosSwine DiseasesAm. J. Vet. Res.American Journal Veterinary Research110114741CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-81051https://bibliotecadigital.udea.edu.co/bitstream/10495/32602/2/license_rdfe2060682c9c70d4d30c83c51448f4eedMD52ORIGINALVillarDavid_2013_Interleukin-4.pdfVillarDavid_2013_Interleukin-4.pdfArtículo de investigaciónapplication/pdf113612https://bibliotecadigital.udea.edu.co/bitstream/10495/32602/1/VillarDavid_2013_Interleukin-4.pdfde04040fb2ae33b56450eef3f29bd927MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://bibliotecadigital.udea.edu.co/bitstream/10495/32602/3/license.txt8a4605be74aa9ea9d79846c1fba20a33MD5310495/32602oai:bibliotecadigital.udea.edu.co:10495/326022022-12-09 13:34:52.288Repositorio Institucional Universidad de Antioquiaandres.perez@udea.edu.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 |