Murine invariant natural killer T cells recognize glycolipids derived from extracts of the lichen Stereocaulon Ramulosum
ABSTRACT: Background: Invariant natural killer t cells (iNkt) can be activated by certain types of glycolipids that have the potential to generate adjuvant effects which could be used to develop effective and safe immunotherapies. Many of these glycolipids have been isolated from natural organisms,...
- Autores:
-
Baena García, Andrés
Gómez Giraldo, Lina
Gómez Henao, Wilton Albeiro
Peláez Jaramillo, Carlos Alberto
- Tipo de recurso:
- Article of investigation
- Fecha de publicación:
- 2015
- Institución:
- Universidad de Antioquia
- Repositorio:
- Repositorio UdeA
- Idioma:
- eng
- OAI Identifier:
- oai:bibliotecadigital.udea.edu.co:10495/25247
- Acceso en línea:
- http://hdl.handle.net/10495/25247
- Palabra clave:
- Células T Asesinas Naturales
Natural Killer T-Cells
Adyuvantes inmunologicos
Immunological adjuvants
Glycolipids
Glicolípidos
Lichens
Líquenes
http://aims.fao.org/aos/agrovoc/c_32670
http://aims.fao.org/aos/agrovoc/c_4314
- Rights
- openAccess
- License
- http://creativecommons.org/licenses/by-nc-sa/2.5/co/
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|
| dc.title.spa.fl_str_mv |
Murine invariant natural killer T cells recognize glycolipids derived from extracts of the lichen Stereocaulon Ramulosum |
| dc.title.alternative.spa.fl_str_mv |
Las células T asesinas naturales invariantes murinas reconocen glicolípidos derivados de extractos del liquen Stereocaulon ramulosum |
| title |
Murine invariant natural killer T cells recognize glycolipids derived from extracts of the lichen Stereocaulon Ramulosum |
| spellingShingle |
Murine invariant natural killer T cells recognize glycolipids derived from extracts of the lichen Stereocaulon Ramulosum Células T Asesinas Naturales Natural Killer T-Cells Adyuvantes inmunologicos Immunological adjuvants Glycolipids Glicolípidos Lichens Líquenes http://aims.fao.org/aos/agrovoc/c_32670 http://aims.fao.org/aos/agrovoc/c_4314 |
| title_short |
Murine invariant natural killer T cells recognize glycolipids derived from extracts of the lichen Stereocaulon Ramulosum |
| title_full |
Murine invariant natural killer T cells recognize glycolipids derived from extracts of the lichen Stereocaulon Ramulosum |
| title_fullStr |
Murine invariant natural killer T cells recognize glycolipids derived from extracts of the lichen Stereocaulon Ramulosum |
| title_full_unstemmed |
Murine invariant natural killer T cells recognize glycolipids derived from extracts of the lichen Stereocaulon Ramulosum |
| title_sort |
Murine invariant natural killer T cells recognize glycolipids derived from extracts of the lichen Stereocaulon Ramulosum |
| dc.creator.fl_str_mv |
Baena García, Andrés Gómez Giraldo, Lina Gómez Henao, Wilton Albeiro Peláez Jaramillo, Carlos Alberto |
| dc.contributor.author.none.fl_str_mv |
Baena García, Andrés Gómez Giraldo, Lina Gómez Henao, Wilton Albeiro Peláez Jaramillo, Carlos Alberto |
| dc.subject.decs.none.fl_str_mv |
Células T Asesinas Naturales Natural Killer T-Cells |
| topic |
Células T Asesinas Naturales Natural Killer T-Cells Adyuvantes inmunologicos Immunological adjuvants Glycolipids Glicolípidos Lichens Líquenes http://aims.fao.org/aos/agrovoc/c_32670 http://aims.fao.org/aos/agrovoc/c_4314 |
| dc.subject.lemb.none.fl_str_mv |
Adyuvantes inmunologicos Immunological adjuvants |
| dc.subject.agrovoc.none.fl_str_mv |
Glycolipids Glicolípidos Lichens Líquenes |
| dc.subject.agrovocuri.none.fl_str_mv |
http://aims.fao.org/aos/agrovoc/c_32670 http://aims.fao.org/aos/agrovoc/c_4314 |
| description |
ABSTRACT: Background: Invariant natural killer t cells (iNkt) can be activated by certain types of glycolipids that have the potential to generate adjuvant effects which could be used to develop effective and safe immunotherapies. Many of these glycolipids have been isolated from natural organisms, but there is a great amount of these organisms completely unexplored as a source of these types of compounds. Some of these organisms are lichens which are complex symbiotic organisms that have been showed to contain glycolipids. Objectives: We decide to test if glycolipids isolated from lichens would be able to activate iNkt cells in vitro and in vivo. Methods: We have used extracted glycolipids from 43 different species of lichens from Colombia. We have used iNkt hybridoma cells, C57BL/6 mice, IL-2 ELISA and the B16 melanoma to test for the adjuvant capabilities of glycolipids isolated from lichens. Results: In this study we have found two glycolipids with the capacity to activate iNkt cells in vitro. one of the glycolipids was able to activate iNkt cells in vivo, and was competent to induce protection against the B16 melanoma in the mouse model. Conclusions: We propose a possible chemical structure for a novel glycolipid called β- GalCer-lich (1) derived from the lichen Stereocaulon ramulosum. |
| publishDate |
2015 |
| dc.date.issued.none.fl_str_mv |
2015 |
| dc.date.accessioned.none.fl_str_mv |
2022-01-13T21:55:40Z |
| dc.date.available.none.fl_str_mv |
2022-01-13T21:55:40Z |
| dc.type.spa.fl_str_mv |
info:eu-repo/semantics/article |
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http://purl.org/coar/version/c_970fb48d4fbd8a85 |
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info:eu-repo/semantics/publishedVersion |
| dc.type.coar.spa.fl_str_mv |
http://purl.org/coar/resource_type/c_2df8fbb1 |
| dc.type.redcol.spa.fl_str_mv |
https://purl.org/redcol/resource_type/ART |
| dc.type.local.spa.fl_str_mv |
Artículo de investigación |
| format |
http://purl.org/coar/resource_type/c_2df8fbb1 |
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publishedVersion |
| dc.identifier.citation.spa.fl_str_mv |
Baena, A., Gómez, L., Gómez, W., & Peláez, C. (2015). Murine invariant natural killer T cells recognize glycolipids derived from extracts of the lichen Stereocaulon Ramulosum. Vitae , 22 (1), 13-26. https://doi.org/10.17533/udea.vitae.v22n1a02 |
| dc.identifier.issn.none.fl_str_mv |
0121-4004 |
| dc.identifier.uri.none.fl_str_mv |
http://hdl.handle.net/10495/25247 |
| dc.identifier.doi.none.fl_str_mv |
10.17533/udea.vitae.v22n1a02 |
| dc.identifier.eissn.none.fl_str_mv |
2145-2660 |
| identifier_str_mv |
Baena, A., Gómez, L., Gómez, W., & Peláez, C. (2015). Murine invariant natural killer T cells recognize glycolipids derived from extracts of the lichen Stereocaulon Ramulosum. Vitae , 22 (1), 13-26. https://doi.org/10.17533/udea.vitae.v22n1a02 0121-4004 10.17533/udea.vitae.v22n1a02 2145-2660 |
| url |
http://hdl.handle.net/10495/25247 |
| dc.language.iso.spa.fl_str_mv |
eng |
| language |
eng |
| dc.relation.ispartofjournalabbrev.spa.fl_str_mv |
Vitae |
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info:eu-repo/semantics/openAccess |
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http://creativecommons.org/licenses/by-nc-sa/2.5/co/ |
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https://creativecommons.org/licenses/by-nc-sa/4.0/ |
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openAccess |
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http://creativecommons.org/licenses/by-nc-sa/2.5/co/ http://purl.org/coar/access_right/c_abf2 https://creativecommons.org/licenses/by-nc-sa/4.0/ |
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14 |
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application/pdf |
| dc.publisher.spa.fl_str_mv |
Universidad de Antioquia, Facultad de Ciencias Farmacéuticas y Alimentarias |
| dc.publisher.group.spa.fl_str_mv |
Grupo de Inmunología Celular e Inmunogenética Grupo Interdisciplinario de Estudios Moleculares |
| dc.publisher.place.spa.fl_str_mv |
Medellín, Colombia |
| institution |
Universidad de Antioquia |
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andres.perez@udea.edu.co |
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Baena García, AndrésGómez Giraldo, LinaGómez Henao, Wilton AlbeiroPeláez Jaramillo, Carlos Alberto2022-01-13T21:55:40Z2022-01-13T21:55:40Z2015Baena, A., Gómez, L., Gómez, W., & Peláez, C. (2015). Murine invariant natural killer T cells recognize glycolipids derived from extracts of the lichen Stereocaulon Ramulosum. Vitae , 22 (1), 13-26. https://doi.org/10.17533/udea.vitae.v22n1a020121-4004http://hdl.handle.net/10495/2524710.17533/udea.vitae.v22n1a022145-2660ABSTRACT: Background: Invariant natural killer t cells (iNkt) can be activated by certain types of glycolipids that have the potential to generate adjuvant effects which could be used to develop effective and safe immunotherapies. Many of these glycolipids have been isolated from natural organisms, but there is a great amount of these organisms completely unexplored as a source of these types of compounds. Some of these organisms are lichens which are complex symbiotic organisms that have been showed to contain glycolipids. Objectives: We decide to test if glycolipids isolated from lichens would be able to activate iNkt cells in vitro and in vivo. Methods: We have used extracted glycolipids from 43 different species of lichens from Colombia. We have used iNkt hybridoma cells, C57BL/6 mice, IL-2 ELISA and the B16 melanoma to test for the adjuvant capabilities of glycolipids isolated from lichens. Results: In this study we have found two glycolipids with the capacity to activate iNkt cells in vitro. one of the glycolipids was able to activate iNkt cells in vivo, and was competent to induce protection against the B16 melanoma in the mouse model. Conclusions: We propose a possible chemical structure for a novel glycolipid called β- GalCer-lich (1) derived from the lichen Stereocaulon ramulosum.RESUMEN: Antecedentes: Las células asesinas naturales t (iNkt) pueden ser activadas por ciertos tipos de glicolípidos que tienen el potencial para generar efectos adyuvantes los cuales pueden ser usados para desarrollar inmunoterapias efectivas. Muchos de estos glicolípidos han sido aislados de organismos naturales, pero hay una gran cantidad de organismos completamente inexplorados como fuente de este tipo de compuestos. Algunos de estos organismos son los líquenes, los cuales son organismos simbiontes complejos para los que se ha mostrado que contienen glicolípidos. Objetivos: Nosotros decidimos probar si los glicolípidos aislados de líquenes podrían ser capaces de activar alas celulas iNkt in vitro e in vivo. Metodos: Nosotros hemos extraído glicolípidos de 43 especies de líquenes de Colombia. Nosotros hemos usado células de un hibridoma de iNkts, ratones C57BL/6, ELISA para IL-2 y el melanoma B16 para probar la capacidad adyuvante de los glicolipidos aislados de los líquenes. Resultados: En este estudio nosotros hemos encontrado dos glicolípidos con la capacidad de activar iNkts in vitro. Uno de los glicolípidos fue capaz de activar células iNkt in vivo, y fue competente para inducir protección contra el melanoma B16 en el modelo de ratón. Conclusiones: Nosotros proponemos una posible estructura química para el nuevo glicolípido llamado β- GalCer-lich (1) derivado del liquen Stereocaulon ramulosum.COL0007462COL000863914application/pdfengUniversidad de Antioquia, Facultad de Ciencias Farmacéuticas y AlimentariasGrupo de Inmunología Celular e InmunogenéticaGrupo Interdisciplinario de Estudios MolecularesMedellín, Colombiainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_2df8fbb1https://purl.org/redcol/resource_type/ARTArtículo de investigaciónhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/2.5/co/http://purl.org/coar/access_right/c_abf2https://creativecommons.org/licenses/by-nc-sa/4.0/Murine invariant natural killer T cells recognize glycolipids derived from extracts of the lichen Stereocaulon RamulosumLas células T asesinas naturales invariantes murinas reconocen glicolípidos derivados de extractos del liquen Stereocaulon ramulosumCélulas T Asesinas NaturalesNatural Killer T-CellsAdyuvantes inmunologicosImmunological adjuvantsGlycolipidsGlicolípidosLichensLíqueneshttp://aims.fao.org/aos/agrovoc/c_32670http://aims.fao.org/aos/agrovoc/c_4314VitaeVitae1326221CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-81051https://bibliotecadigital.udea.edu.co/bitstream/10495/25247/2/license_rdfe2060682c9c70d4d30c83c51448f4eedMD52LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://bibliotecadigital.udea.edu.co/bitstream/10495/25247/3/license.txt8a4605be74aa9ea9d79846c1fba20a33MD53ORIGINALBaenaAndrés_2015_KillerTCellsGlycolipids.pdfBaenaAndrés_2015_KillerTCellsGlycolipids.pdfArtículo de investigaciónapplication/pdf1046844https://bibliotecadigital.udea.edu.co/bitstream/10495/25247/1/BaenaAndr%c3%a9s_2015_KillerTCellsGlycolipids.pdf2cdf1a8cf6d742874e66a2d325ce4dcbMD5110495/25247oai:bibliotecadigital.udea.edu.co:10495/252472023-04-11 16:10:15.742Repositorio Institucional Universidad de Antioquiaandres.perez@udea.edu.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 |