Murine invariant natural killer T cells recognize glycolipids derived from extracts of the lichen Stereocaulon Ramulosum

ABSTRACT: Background: Invariant natural killer t cells (iNkt) can be activated by certain types of glycolipids that have the potential to generate adjuvant effects which could be used to develop effective and safe immunotherapies. Many of these glycolipids have been isolated from natural organisms,...

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Autores:
Baena García, Andrés
Gómez Giraldo, Lina
Gómez Henao, Wilton Albeiro
Peláez Jaramillo, Carlos Alberto
Tipo de recurso:
Article of investigation
Fecha de publicación:
2015
Institución:
Universidad de Antioquia
Repositorio:
Repositorio UdeA
Idioma:
eng
OAI Identifier:
oai:bibliotecadigital.udea.edu.co:10495/25247
Acceso en línea:
http://hdl.handle.net/10495/25247
Palabra clave:
Células T Asesinas Naturales
Natural Killer T-Cells
Adyuvantes inmunologicos
Immunological adjuvants
Glycolipids
Glicolípidos
Lichens
Líquenes
http://aims.fao.org/aos/agrovoc/c_32670
http://aims.fao.org/aos/agrovoc/c_4314
Rights
openAccess
License
http://creativecommons.org/licenses/by-nc-sa/2.5/co/
Description
Summary:ABSTRACT: Background: Invariant natural killer t cells (iNkt) can be activated by certain types of glycolipids that have the potential to generate adjuvant effects which could be used to develop effective and safe immunotherapies. Many of these glycolipids have been isolated from natural organisms, but there is a great amount of these organisms completely unexplored as a source of these types of compounds. Some of these organisms are lichens which are complex symbiotic organisms that have been showed to contain glycolipids. Objectives: We decide to test if glycolipids isolated from lichens would be able to activate iNkt cells in vitro and in vivo. Methods: We have used extracted glycolipids from 43 different species of lichens from Colombia. We have used iNkt hybridoma cells, C57BL/6 mice, IL-2 ELISA and the B16 melanoma to test for the adjuvant capabilities of glycolipids isolated from lichens. Results: In this study we have found two glycolipids with the capacity to activate iNkt cells in vitro. one of the glycolipids was able to activate iNkt cells in vivo, and was competent to induce protection against the B16 melanoma in the mouse model. Conclusions: We propose a possible chemical structure for a novel glycolipid called β- GalCer-lich (1) derived from the lichen Stereocaulon ramulosum.