Optimization of culture conditions to obtain maximal growth of penicillin-resistant Streptococcus pneumoniae

ABSTRACT: Background Streptococcus pneumoniae, particularly penicillin-resistant strains (PRSP), constitute one of the most important causes of serious infections worldwide. It is a fastidious microorganism with exquisite nutritional and environmental requirements to grow, a characteristic that prev...

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Autores:
Restrepo Gouzy, Andrea Victoria
Salazar Giraldo, Beatriz
Agudelo Pérez, María
Rodríguez Jaramillo, Carlos Andrés
Zuluaga Salazar, Andrés Felipe
Vesga Meneses, Omar
Tipo de recurso:
Article of investigation
Fecha de publicación:
2005
Institución:
Universidad de Antioquia
Repositorio:
Repositorio UdeA
Idioma:
eng
OAI Identifier:
oai:bibliotecadigital.udea.edu.co:10495/25776
Acceso en línea:
http://hdl.handle.net/10495/25776
Palabra clave:
Streptococcus pneumoniae
Penicilinas
Penicillins
Rights
openAccess
License
http://creativecommons.org/licenses/by/2.5/co/
id UDEA2_52c2cd51027bc766fa004c94c0035eff
oai_identifier_str oai:bibliotecadigital.udea.edu.co:10495/25776
network_acronym_str UDEA2
network_name_str Repositorio UdeA
repository_id_str
dc.title.spa.fl_str_mv Optimization of culture conditions to obtain maximal growth of penicillin-resistant Streptococcus pneumoniae
title Optimization of culture conditions to obtain maximal growth of penicillin-resistant Streptococcus pneumoniae
spellingShingle Optimization of culture conditions to obtain maximal growth of penicillin-resistant Streptococcus pneumoniae
Streptococcus pneumoniae
Penicilinas
Penicillins
title_short Optimization of culture conditions to obtain maximal growth of penicillin-resistant Streptococcus pneumoniae
title_full Optimization of culture conditions to obtain maximal growth of penicillin-resistant Streptococcus pneumoniae
title_fullStr Optimization of culture conditions to obtain maximal growth of penicillin-resistant Streptococcus pneumoniae
title_full_unstemmed Optimization of culture conditions to obtain maximal growth of penicillin-resistant Streptococcus pneumoniae
title_sort Optimization of culture conditions to obtain maximal growth of penicillin-resistant Streptococcus pneumoniae
dc.creator.fl_str_mv Restrepo Gouzy, Andrea Victoria
Salazar Giraldo, Beatriz
Agudelo Pérez, María
Rodríguez Jaramillo, Carlos Andrés
Zuluaga Salazar, Andrés Felipe
Vesga Meneses, Omar
dc.contributor.author.none.fl_str_mv Restrepo Gouzy, Andrea Victoria
Salazar Giraldo, Beatriz
Agudelo Pérez, María
Rodríguez Jaramillo, Carlos Andrés
Zuluaga Salazar, Andrés Felipe
Vesga Meneses, Omar
dc.subject.decs.none.fl_str_mv Streptococcus pneumoniae
Penicilinas
Penicillins
topic Streptococcus pneumoniae
Penicilinas
Penicillins
description ABSTRACT: Background Streptococcus pneumoniae, particularly penicillin-resistant strains (PRSP), constitute one of the most important causes of serious infections worldwide. It is a fastidious microorganism with exquisite nutritional and environmental requirements to grow, a characteristic that prevents the development of useful animal models to study the biology of the microorganism. This study was designed to determine optimal conditions for culture and growth of PRSP. Results We developed a simple and reproducible method for culture of diverse strains of PRSP representing several invasive serotypes of clinical and epidemiological importance in Colombia. Application of this 3-step culture protocol consistently produced more than 9 log10 CFU/ml of viable cells in the middle part of the logarithmic phase of their growth curve. Conclusion A controlled inoculum size grown in 3 successive steps in supplemented agar and broth under 5% CO2 atmosphere, with pH adjustment and specific incubation times, allowed production of great numbers of PRSP without untimely activation of autolysis mechanisms.
publishDate 2005
dc.date.issued.none.fl_str_mv 2005
dc.date.accessioned.none.fl_str_mv 2022-02-03T18:52:09Z
dc.date.available.none.fl_str_mv 2022-02-03T18:52:09Z
dc.type.spa.fl_str_mv info:eu-repo/semantics/article
dc.type.coarversion.fl_str_mv http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.hasversion.spa.fl_str_mv info:eu-repo/semantics/publishedVersion
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dc.type.local.spa.fl_str_mv Artículo de investigación
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dc.identifier.uri.none.fl_str_mv http://hdl.handle.net/10495/25776
dc.identifier.eissn.none.fl_str_mv 1471-2180
url http://hdl.handle.net/10495/25776
identifier_str_mv 1471-2180
dc.language.iso.spa.fl_str_mv eng
language eng
dc.relation.ispartofjournalabbrev.spa.fl_str_mv BMC Microbiol.
dc.rights.spa.fl_str_mv info:eu-repo/semantics/openAccess
dc.rights.uri.*.fl_str_mv http://creativecommons.org/licenses/by/2.5/co/
dc.rights.accessrights.spa.fl_str_mv http://purl.org/coar/access_right/c_abf2
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dc.format.extent.spa.fl_str_mv 8
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dc.publisher.spa.fl_str_mv BMC
dc.publisher.group.spa.fl_str_mv GRIPE: Grupo Investigador de Problemas en Enfermedades Infecciosas
dc.publisher.place.spa.fl_str_mv Londres, Inglaterra
institution Universidad de Antioquia
bitstream.url.fl_str_mv http://bibliotecadigital.udea.edu.co/bitstream/10495/25776/1/RestrepoAndrea_OptimizationCultureConditions.pdf
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repository.name.fl_str_mv Repositorio Institucional Universidad de Antioquia
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spelling Restrepo Gouzy, Andrea VictoriaSalazar Giraldo, BeatrizAgudelo Pérez, MaríaRodríguez Jaramillo, Carlos AndrésZuluaga Salazar, Andrés FelipeVesga Meneses, Omar2022-02-03T18:52:09Z2022-02-03T18:52:09Z2005http://hdl.handle.net/10495/257761471-2180ABSTRACT: Background Streptococcus pneumoniae, particularly penicillin-resistant strains (PRSP), constitute one of the most important causes of serious infections worldwide. It is a fastidious microorganism with exquisite nutritional and environmental requirements to grow, a characteristic that prevents the development of useful animal models to study the biology of the microorganism. This study was designed to determine optimal conditions for culture and growth of PRSP. Results We developed a simple and reproducible method for culture of diverse strains of PRSP representing several invasive serotypes of clinical and epidemiological importance in Colombia. Application of this 3-step culture protocol consistently produced more than 9 log10 CFU/ml of viable cells in the middle part of the logarithmic phase of their growth curve. Conclusion A controlled inoculum size grown in 3 successive steps in supplemented agar and broth under 5% CO2 atmosphere, with pH adjustment and specific incubation times, allowed production of great numbers of PRSP without untimely activation of autolysis mechanisms.COL00057448application/pdfengBMCGRIPE: Grupo Investigador de Problemas en Enfermedades InfecciosasLondres, Inglaterrainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_2df8fbb1https://purl.org/redcol/resource_type/ARTArtículo de investigaciónhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/2.5/co/http://purl.org/coar/access_right/c_abf2https://creativecommons.org/licenses/by/4.0/Optimization of culture conditions to obtain maximal growth of penicillin-resistant Streptococcus pneumoniaeStreptococcus pneumoniaePenicilinasPenicillinsBMC Microbiol.BMC Microbiology185ORIGINALRestrepoAndrea_OptimizationCultureConditions.pdfRestrepoAndrea_OptimizationCultureConditions.pdfArtículo de investigaciónapplication/pdf1553167http://bibliotecadigital.udea.edu.co/bitstream/10495/25776/1/RestrepoAndrea_OptimizationCultureConditions.pdf325662811a049ca50e0a3002322d9072MD51CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8927http://bibliotecadigital.udea.edu.co/bitstream/10495/25776/2/license_rdf1646d1f6b96dbbbc38035efc9239ac9cMD52LICENSElicense.txtlicense.txttext/plain; charset=utf-81748http://bibliotecadigital.udea.edu.co/bitstream/10495/25776/3/license.txt8a4605be74aa9ea9d79846c1fba20a33MD5310495/25776oai:bibliotecadigital.udea.edu.co:10495/257762022-02-03 13:52:09.806Repositorio Institucional Universidad de Antioquiaandres.perez@udea.edu.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