Identification of Brucella canis Group 2 in colombian kennels

ABSTRACT: recently, isolates of Brucella canis (B. canis) were classified into two groups, Group 1 and Group 2, based on a 794 bp deletion in the polysaccharide deacetylase gene in Group 1, or absence of this deletion in Group 2. In Colombia, B. canis was first isolated from sick or clinically healthy seropositive dogs in 2005. Objective: to determine Brucella canis genotype in isolates from Medellín Metropolitan Area. Methods: 49 isolates from a study involving 193 dogs in 10 kennels were analyzed by Polymerase Chain Reaction (PCR) using three primer pairs of eight previously reported. Results: all isolates were positive for 152, 272, and 794 bp fragments, indicating that they belong to Group 2. Conclusion: this article presents strong evidence based on the new molecular marker used to classify B. canis isolates from Medellín (Colombia) into Group 2. This is a pioneer molecular study on the presence of B. canis in Colombia and it would be interesting to apply this method to characterize the infection epidemiology and explore its domestic clinical dynamics.

• Autores: Ortiz Román, Luisa Fernanda; Muskus López, Carlos Enrique; Sánchez Jiménez, Miryan Margot; Olivera Ángel, Martha
• Tipo de recurso: Article of investigation
• Fecha de publicación: 2012
• Institución: Universidad de Antioquia
• Repositorio: Repositorio UdeA
• Idioma: eng
• OAI Identifier: oai:bibliotecadigital.udea.edu.co:10495/8075
• Acceso en línea: http://hdl.handle.net/10495/8075
• Rights: openAccess
• License: Atribución-NoComercial-CompartirIgual 2.5 Colombia (CC BY-NC-SA 2.5 CO)