Immunological identification of Trypanosoma cruzi lineages in human infection along the endemic area
ABSTRACT: Genotyping studies show a polarized geographic distribution of Trypanosoma cruzi lineages in humans. Here, we assessed their distribution along Latin America through an immunological approach we designated Western blot (WB) assay with Trypomastigote small-surface antigen (TSSA) I and TSSA...
- Autores:
-
Risso, Marikena
Sartor, Paula
Burgos, Juan
Briceño, Luis
Rodríguez, Eva
Guhl, Felipe
Triana Chávez, Omar
Espinoza, Berta
Monteón Padilla, Víctor Manuel
Russomando, Graciela
Schijman, Alejandro
Bottasso, Oscar
Leguizamon, María Susana
- Tipo de recurso:
- Article of investigation
- Fecha de publicación:
- 2011
- Institución:
- Universidad de Antioquia
- Repositorio:
- Repositorio UdeA
- Idioma:
- eng
- OAI Identifier:
- oai:bibliotecadigital.udea.edu.co:10495/26765
- Acceso en línea:
- http://hdl.handle.net/10495/26765
- Palabra clave:
- Enfermedad de Chagas
Chagas Disease
Enfermedades Endémicas
Endemic Diseases
Trypanosoma cruzi
América Latina
Latin America
Anticuerpos Antiprotozoarios - sangre
Antibodies, Protozoan - blood
Antígenos de Protozoos
Antigens, Protozoan
Western Blotting
Blotting, Western
Inmunofenotipificación
Immunophenotyping
- Rights
- openAccess
- License
- http://creativecommons.org/licenses/by/2.5/co/
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|
| dc.title.spa.fl_str_mv |
Immunological identification of Trypanosoma cruzi lineages in human infection along the endemic area |
| title |
Immunological identification of Trypanosoma cruzi lineages in human infection along the endemic area |
| spellingShingle |
Immunological identification of Trypanosoma cruzi lineages in human infection along the endemic area Enfermedad de Chagas Chagas Disease Enfermedades Endémicas Endemic Diseases Trypanosoma cruzi América Latina Latin America Anticuerpos Antiprotozoarios - sangre Antibodies, Protozoan - blood Antígenos de Protozoos Antigens, Protozoan Western Blotting Blotting, Western Inmunofenotipificación Immunophenotyping |
| title_short |
Immunological identification of Trypanosoma cruzi lineages in human infection along the endemic area |
| title_full |
Immunological identification of Trypanosoma cruzi lineages in human infection along the endemic area |
| title_fullStr |
Immunological identification of Trypanosoma cruzi lineages in human infection along the endemic area |
| title_full_unstemmed |
Immunological identification of Trypanosoma cruzi lineages in human infection along the endemic area |
| title_sort |
Immunological identification of Trypanosoma cruzi lineages in human infection along the endemic area |
| dc.creator.fl_str_mv |
Risso, Marikena Sartor, Paula Burgos, Juan Briceño, Luis Rodríguez, Eva Guhl, Felipe Triana Chávez, Omar Espinoza, Berta Monteón Padilla, Víctor Manuel Russomando, Graciela Schijman, Alejandro Bottasso, Oscar Leguizamon, María Susana |
| dc.contributor.author.none.fl_str_mv |
Risso, Marikena Sartor, Paula Burgos, Juan Briceño, Luis Rodríguez, Eva Guhl, Felipe Triana Chávez, Omar Espinoza, Berta Monteón Padilla, Víctor Manuel Russomando, Graciela Schijman, Alejandro Bottasso, Oscar Leguizamon, María Susana |
| dc.subject.decs.none.fl_str_mv |
Enfermedad de Chagas Chagas Disease Enfermedades Endémicas Endemic Diseases Trypanosoma cruzi América Latina Latin America Anticuerpos Antiprotozoarios - sangre Antibodies, Protozoan - blood Antígenos de Protozoos Antigens, Protozoan Western Blotting Blotting, Western Inmunofenotipificación Immunophenotyping |
| topic |
Enfermedad de Chagas Chagas Disease Enfermedades Endémicas Endemic Diseases Trypanosoma cruzi América Latina Latin America Anticuerpos Antiprotozoarios - sangre Antibodies, Protozoan - blood Antígenos de Protozoos Antigens, Protozoan Western Blotting Blotting, Western Inmunofenotipificación Immunophenotyping |
| description |
ABSTRACT: Genotyping studies show a polarized geographic distribution of Trypanosoma cruzi lineages in humans. Here, we assessed their distribution along Latin America through an immunological approach we designated Western blot (WB) assay with Trypomastigote small-surface antigen (TSSA) I and TSSA II (TSSA-WB). These antigens are expressed by T. cruzi I (TCI; now TcI) and T. cruzi II (TCII; reclassified as TcII to TcVI) parasites. TSSA-WB showed good concordance with genotyping tests. An unexpected frequency of TSSA II recognition was observed in Colombia, Venezuela, and Mexico (northern region of Latin America). In Argentina and Paraguay (southern region), immunophenotyping confirmed the already reported TCII (TcII to TcVI) dominance. The lineage distribution between these regions showed significant difference but not among countries within them (except for Colombia and Venezuela). TSSA-WB shows TCII emergence in the northern region where TCI was reported as dominant or even as the unique T. cruzi lineage infecting humans. |
| publishDate |
2011 |
| dc.date.issued.none.fl_str_mv |
2011 |
| dc.date.accessioned.none.fl_str_mv |
2022-03-22T17:05:42Z |
| dc.date.available.none.fl_str_mv |
2022-03-22T17:05:42Z |
| dc.type.spa.fl_str_mv |
info:eu-repo/semantics/article |
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http://purl.org/coar/version/c_970fb48d4fbd8a85 |
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info:eu-repo/semantics/publishedVersion |
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http://purl.org/coar/resource_type/c_2df8fbb1 |
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https://purl.org/redcol/resource_type/ART |
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Artículo de investigación |
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http://purl.org/coar/resource_type/c_2df8fbb1 |
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publishedVersion |
| dc.identifier.citation.spa.fl_str_mv |
Risso, M., Sartor, P. A., Burgos, J., Briceño, L., Rodríguez, E., Guhl, F., Triana, O., Espinoza, B., Monteón, V., Russomando, G., Schijman, A., Bottasso, O., & Leguizamón, M. (2011). Immunological identification of Trypanosoma cruzi lineages in human infection along the endemic area. The American journal of tropical medicine and hygiene, 84(1), 78–84. https://doi.org/10.4269/ajtmh.2011.10-0177 |
| dc.identifier.issn.none.fl_str_mv |
0002-9637 |
| dc.identifier.uri.none.fl_str_mv |
http://hdl.handle.net/10495/26765 |
| dc.identifier.doi.none.fl_str_mv |
10.4269/ajtmh.2011.10-0177 |
| dc.identifier.eissn.none.fl_str_mv |
1476-1645 |
| identifier_str_mv |
Risso, M., Sartor, P. A., Burgos, J., Briceño, L., Rodríguez, E., Guhl, F., Triana, O., Espinoza, B., Monteón, V., Russomando, G., Schijman, A., Bottasso, O., & Leguizamón, M. (2011). Immunological identification of Trypanosoma cruzi lineages in human infection along the endemic area. The American journal of tropical medicine and hygiene, 84(1), 78–84. https://doi.org/10.4269/ajtmh.2011.10-0177 0002-9637 10.4269/ajtmh.2011.10-0177 1476-1645 |
| url |
http://hdl.handle.net/10495/26765 |
| dc.language.iso.spa.fl_str_mv |
eng |
| language |
eng |
| dc.relation.ispartofjournalabbrev.spa.fl_str_mv |
Am. J. Trop. Med. Hyg. |
| dc.rights.spa.fl_str_mv |
info:eu-repo/semantics/openAccess |
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http://creativecommons.org/licenses/by/2.5/co/ |
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https://creativecommons.org/licenses/by/4.0/ |
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openAccess |
| rights_invalid_str_mv |
http://creativecommons.org/licenses/by/2.5/co/ http://purl.org/coar/access_right/c_abf2 https://creativecommons.org/licenses/by/4.0/ |
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application/pdf |
| dc.publisher.spa.fl_str_mv |
American Society of Tropical Medicine and Hygiene |
| dc.publisher.group.spa.fl_str_mv |
Biología y Control de Enfermedades Infecciosas |
| dc.publisher.place.spa.fl_str_mv |
Baltimore, Estados Unidos |
| institution |
Universidad de Antioquia |
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Risso, MarikenaSartor, PaulaBurgos, JuanBriceño, LuisRodríguez, EvaGuhl, FelipeTriana Chávez, OmarEspinoza, BertaMonteón Padilla, Víctor ManuelRussomando, GracielaSchijman, AlejandroBottasso, OscarLeguizamon, María Susana2022-03-22T17:05:42Z2022-03-22T17:05:42Z2011Risso, M., Sartor, P. A., Burgos, J., Briceño, L., Rodríguez, E., Guhl, F., Triana, O., Espinoza, B., Monteón, V., Russomando, G., Schijman, A., Bottasso, O., & Leguizamón, M. (2011). Immunological identification of Trypanosoma cruzi lineages in human infection along the endemic area. The American journal of tropical medicine and hygiene, 84(1), 78–84. https://doi.org/10.4269/ajtmh.2011.10-01770002-9637http://hdl.handle.net/10495/2676510.4269/ajtmh.2011.10-01771476-1645ABSTRACT: Genotyping studies show a polarized geographic distribution of Trypanosoma cruzi lineages in humans. Here, we assessed their distribution along Latin America through an immunological approach we designated Western blot (WB) assay with Trypomastigote small-surface antigen (TSSA) I and TSSA II (TSSA-WB). These antigens are expressed by T. cruzi I (TCI; now TcI) and T. cruzi II (TCII; reclassified as TcII to TcVI) parasites. TSSA-WB showed good concordance with genotyping tests. An unexpected frequency of TSSA II recognition was observed in Colombia, Venezuela, and Mexico (northern region of Latin America). In Argentina and Paraguay (southern region), immunophenotyping confirmed the already reported TCII (TcII to TcVI) dominance. The lineage distribution between these regions showed significant difference but not among countries within them (except for Colombia and Venezuela). TSSA-WB shows TCII emergence in the northern region where TCI was reported as dominant or even as the unique T. cruzi lineage infecting humans.COL00078657application/pdfengAmerican Society of Tropical Medicine and HygieneBiología y Control de Enfermedades InfecciosasBaltimore, Estados Unidosinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_2df8fbb1https://purl.org/redcol/resource_type/ARTArtículo de investigaciónhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/2.5/co/http://purl.org/coar/access_right/c_abf2https://creativecommons.org/licenses/by/4.0/Immunological identification of Trypanosoma cruzi lineages in human infection along the endemic areaEnfermedad de ChagasChagas DiseaseEnfermedades EndémicasEndemic DiseasesTrypanosoma cruziAmérica LatinaLatin AmericaAnticuerpos Antiprotozoarios - sangreAntibodies, Protozoan - bloodAntígenos de ProtozoosAntigens, ProtozoanWestern BlottingBlotting, WesternInmunofenotipificaciónImmunophenotypingAm. J. Trop. Med. Hyg.American Journal of Tropical Medicine and Hygiene7884841ORIGINALTrianaOmar_2011_ImmunologicalIdentificationTrypanosoma.pdfTrianaOmar_2011_ImmunologicalIdentificationTrypanosoma.pdfArtículo de investigaciónapplication/pdf924508http://bibliotecadigital.udea.edu.co/bitstream/10495/26765/1/TrianaOmar_2011_ImmunologicalIdentificationTrypanosoma.pdf72c6b78bab9091ca00d080e1fa2d74bdMD51CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8927http://bibliotecadigital.udea.edu.co/bitstream/10495/26765/2/license_rdf1646d1f6b96dbbbc38035efc9239ac9cMD52LICENSElicense.txtlicense.txttext/plain; charset=utf-81748http://bibliotecadigital.udea.edu.co/bitstream/10495/26765/3/license.txt8a4605be74aa9ea9d79846c1fba20a33MD5310495/26765oai:bibliotecadigital.udea.edu.co:10495/267652022-03-22 12:05:43.052Repositorio Institucional Universidad de Antioquiaandres.perez@udea.edu.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 |