Nitric oxide and malondialdehyde concentration in gastric contents and blood in an equine model of gastric ulcer induced by phenylbutazone
ABSTRACT: mechanisms of gastric mucosal injury include cellular damage by oxygen free radicals, which can be indirectly measured through malondialdehyde (MDA). Production of nitric oxide (NO) maintains gastric tissue perfusion through vasodilatation. Objective: to demonstrate oxidative stress and im...
- Autores:
-
Zuluaga Cabrera, Angélica María
Martínez Aranzalez, José Ramón
Silveira Alves, Geraldo Eleno
- Tipo de recurso:
- Article of investigation
- Fecha de publicación:
- 2016
- Institución:
- Universidad de Antioquia
- Repositorio:
- Repositorio UdeA
- Idioma:
- eng
- OAI Identifier:
- oai:bibliotecadigital.udea.edu.co:10495/8057
- Acceso en línea:
- http://hdl.handle.net/10495/8057
- Palabra clave:
- Rights
- openAccess
- License
- Atribución-NoComercial-CompartirIgual 2.5 Colombia (CC BY-NC-SA 2.5 CO)
| id |
UDEA2_48b92001d909bc70e64a9f01d7b0a8d7 |
|---|---|
| oai_identifier_str |
oai:bibliotecadigital.udea.edu.co:10495/8057 |
| network_acronym_str |
UDEA2 |
| network_name_str |
Repositorio UdeA |
| repository_id_str |
|
| dc.title.spa.fl_str_mv |
Nitric oxide and malondialdehyde concentration in gastric contents and blood in an equine model of gastric ulcer induced by phenylbutazone |
| dc.title.alternative.spa.fl_str_mv |
Concentración de óxido nítrico y malonaldehído en contenido gástrico y en sangre en un modelo de úlcera gástrica equina inducida por fenilbutazona |
| title |
Nitric oxide and malondialdehyde concentration in gastric contents and blood in an equine model of gastric ulcer induced by phenylbutazone |
| spellingShingle |
Nitric oxide and malondialdehyde concentration in gastric contents and blood in an equine model of gastric ulcer induced by phenylbutazone |
| title_short |
Nitric oxide and malondialdehyde concentration in gastric contents and blood in an equine model of gastric ulcer induced by phenylbutazone |
| title_full |
Nitric oxide and malondialdehyde concentration in gastric contents and blood in an equine model of gastric ulcer induced by phenylbutazone |
| title_fullStr |
Nitric oxide and malondialdehyde concentration in gastric contents and blood in an equine model of gastric ulcer induced by phenylbutazone |
| title_full_unstemmed |
Nitric oxide and malondialdehyde concentration in gastric contents and blood in an equine model of gastric ulcer induced by phenylbutazone |
| title_sort |
Nitric oxide and malondialdehyde concentration in gastric contents and blood in an equine model of gastric ulcer induced by phenylbutazone |
| dc.creator.fl_str_mv |
Zuluaga Cabrera, Angélica María Martínez Aranzalez, José Ramón Silveira Alves, Geraldo Eleno |
| dc.contributor.author.none.fl_str_mv |
Zuluaga Cabrera, Angélica María Martínez Aranzalez, José Ramón Silveira Alves, Geraldo Eleno |
| description |
ABSTRACT: mechanisms of gastric mucosal injury include cellular damage by oxygen free radicals, which can be indirectly measured through malondialdehyde (MDA). Production of nitric oxide (NO) maintains gastric tissue perfusion through vasodilatation. Objective: to demonstrate oxidative stress and impaired gastric perfusion measuring NO and MDA in gastric contents and blood of equines subjected to a gastrointestinal ulceration induction protocol. Methods: a gastrointestinal ulceration induction protocol involving fasting and oral administration of phenylbutazone was performed on five horses. NO and MDA were measured before and after protocol induction and presence of fecal occult blood (FOB) was evaluated. Animals underwent gastroscopy at the beginning and end of the protocol. Results: horses presented variability in hematological and FOB exams. Azotemia, hyperphosphatemia, and hypocalcemia were found in all animals. No significant changes were found in enzymatic activity. At the end of the protocol, 40% of the horses showed varying degrees of gastric ulceration. NO production in the stomach decreased by 60%, whereas MDA production increased by 55% after the protocol. Plasma concentration of MDA average increased 96 hours after starting the protocol. There were no significant differences in mean plasma NO during the protocol. Conclusion: the protocol used to induce gastric ulcers produces diminished cytoprotection (by NO) and induces oxidative stress in the gastric mucosa. |
| publishDate |
2016 |
| dc.date.issued.none.fl_str_mv |
2016 |
| dc.date.accessioned.none.fl_str_mv |
2017-08-30T16:08:59Z |
| dc.date.available.none.fl_str_mv |
2017-08-30T16:08:59Z |
| dc.type.spa.fl_str_mv |
info:eu-repo/semantics/article |
| dc.type.coarversion.fl_str_mv |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| dc.type.hasversion.spa.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| dc.type.coar.spa.fl_str_mv |
http://purl.org/coar/resource_type/c_2df8fbb1 |
| dc.type.redcol.spa.fl_str_mv |
https://purl.org/redcol/resource_type/ART |
| dc.type.local.spa.fl_str_mv |
Artículo de investigación |
| format |
http://purl.org/coar/resource_type/c_2df8fbb1 |
| status_str |
publishedVersion |
| dc.identifier.citation.spa.fl_str_mv |
Zuluaga AM, Martínez Aranzalez JR, Silveira A GE. Nitric oxide and malondialdehyde concentration in gastric contents and blood in an equine model of gastric ulcer induced by phenylbutazone. Rev. Colomb. Cienc. Pecu. 2016 Mar; 29(1): 43-50. DOI: 10.17533/udea.rccp.v29n1a05 |
| dc.identifier.issn.none.fl_str_mv |
0120-0690 |
| dc.identifier.uri.none.fl_str_mv |
http://hdl.handle.net/10495/8057 |
| dc.identifier.doi.none.fl_str_mv |
10.17533/udea.rccp.v29n1a05 |
| dc.identifier.eissn.none.fl_str_mv |
2256-2958 |
| identifier_str_mv |
Zuluaga AM, Martínez Aranzalez JR, Silveira A GE. Nitric oxide and malondialdehyde concentration in gastric contents and blood in an equine model of gastric ulcer induced by phenylbutazone. Rev. Colomb. Cienc. Pecu. 2016 Mar; 29(1): 43-50. DOI: 10.17533/udea.rccp.v29n1a05 0120-0690 10.17533/udea.rccp.v29n1a05 2256-2958 |
| url |
http://hdl.handle.net/10495/8057 |
| dc.language.iso.spa.fl_str_mv |
eng |
| language |
eng |
| dc.relation.ispartofjournalabbrev.spa.fl_str_mv |
Rev. Colomb. Cienc. Pecu. |
| dc.rights.*.fl_str_mv |
Atribución-NoComercial-CompartirIgual 2.5 Colombia (CC BY-NC-SA 2.5 CO) |
| dc.rights.spa.fl_str_mv |
info:eu-repo/semantics/openAccess |
| dc.rights.uri.*.fl_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/co/ |
| dc.rights.accessrights.spa.fl_str_mv |
http://purl.org/coar/access_right/c_abf2 |
| dc.rights.creativecommons.spa.fl_str_mv |
https://creativecommons.org/licenses/by-nc-sa/4.0/ |
| rights_invalid_str_mv |
Atribución-NoComercial-CompartirIgual 2.5 Colombia (CC BY-NC-SA 2.5 CO) https://creativecommons.org/licenses/by-nc-sa/2.5/co/ http://purl.org/coar/access_right/c_abf2 https://creativecommons.org/licenses/by-nc-sa/4.0/ |
| eu_rights_str_mv |
openAccess |
| dc.format.mimetype.spa.fl_str_mv |
application/pdf |
| dc.publisher.spa.fl_str_mv |
Universidad de Antioquia, Facultad de Ciencias Agrarias |
| dc.publisher.group.spa.fl_str_mv |
CENTAURO |
| dc.publisher.place.spa.fl_str_mv |
Medellín, Colombia |
| institution |
Universidad de Antioquia |
| bitstream.url.fl_str_mv |
http://bibliotecadigital.udea.edu.co/bitstream/10495/8057/1/Zuluaga_A_2016_Nitric_oxide_malondialdehyde.pdf http://bibliotecadigital.udea.edu.co/bitstream/10495/8057/2/license_url http://bibliotecadigital.udea.edu.co/bitstream/10495/8057/3/license_text http://bibliotecadigital.udea.edu.co/bitstream/10495/8057/4/license_rdf http://bibliotecadigital.udea.edu.co/bitstream/10495/8057/5/license.txt |
| bitstream.checksum.fl_str_mv |
2b61422ce4d16cc83e45871ed935a36c 4afdbb8c545fd630ea7db775da747b2f d41d8cd98f00b204e9800998ecf8427e d41d8cd98f00b204e9800998ecf8427e 8a4605be74aa9ea9d79846c1fba20a33 |
| bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 MD5 MD5 MD5 |
| repository.name.fl_str_mv |
Repositorio Institucional Universidad de Antioquia |
| repository.mail.fl_str_mv |
andres.perez@udea.edu.co |
| _version_ |
1812173247906578432 |
| spelling |
Zuluaga Cabrera, Angélica MaríaMartínez Aranzalez, José RamónSilveira Alves, Geraldo Eleno2017-08-30T16:08:59Z2017-08-30T16:08:59Z2016Zuluaga AM, Martínez Aranzalez JR, Silveira A GE. Nitric oxide and malondialdehyde concentration in gastric contents and blood in an equine model of gastric ulcer induced by phenylbutazone. Rev. Colomb. Cienc. Pecu. 2016 Mar; 29(1): 43-50. DOI: 10.17533/udea.rccp.v29n1a050120-0690http://hdl.handle.net/10495/805710.17533/udea.rccp.v29n1a052256-2958ABSTRACT: mechanisms of gastric mucosal injury include cellular damage by oxygen free radicals, which can be indirectly measured through malondialdehyde (MDA). Production of nitric oxide (NO) maintains gastric tissue perfusion through vasodilatation. Objective: to demonstrate oxidative stress and impaired gastric perfusion measuring NO and MDA in gastric contents and blood of equines subjected to a gastrointestinal ulceration induction protocol. Methods: a gastrointestinal ulceration induction protocol involving fasting and oral administration of phenylbutazone was performed on five horses. NO and MDA were measured before and after protocol induction and presence of fecal occult blood (FOB) was evaluated. Animals underwent gastroscopy at the beginning and end of the protocol. Results: horses presented variability in hematological and FOB exams. Azotemia, hyperphosphatemia, and hypocalcemia were found in all animals. No significant changes were found in enzymatic activity. At the end of the protocol, 40% of the horses showed varying degrees of gastric ulceration. NO production in the stomach decreased by 60%, whereas MDA production increased by 55% after the protocol. Plasma concentration of MDA average increased 96 hours after starting the protocol. There were no significant differences in mean plasma NO during the protocol. Conclusion: the protocol used to induce gastric ulcers produces diminished cytoprotection (by NO) and induces oxidative stress in the gastric mucosa.RESUMEN: los mecanismos de lesión de la mucosa gástrica incluyen daño celular por radicales libres de oxígeno que puede ser medido a través del malonaldehído (MDA). La producción de óxido nítrico (NO) sostiene la perfusión del tejido gástrico mediante vasodilatación. Objetivo: demostrar el estrés oxidativo y alteración de la perfusión gástrica mediante la medición de NO y MDA en contenido gástrico y sangre de equinos sometidos a un protocolo de inducción de ulceración gastrointestinal. Métodos: el protocolo de ulceración se hizo en 5 caballos con fenilbutazona y periodos de ayuno. Antes y después de aplicar el protocolo fueron medidos NO y MDA y se evaluó la presencia de sangre oculta en heces (FOB). Los animales fueron sometidos a gastroscopia al inicio y final del protocolo. Resultados: los equinos presentaron variabilidad en los exámenes hematológicos y FOB. Se encontró Azotemia, hiperfosfatemia e hipocalcemia en todos los animales. No fueron encontradas alteraciones significativas en la actividad enzimática. Al final del protocolo, el 40% de los equinos presentó ulceración gástrica en grado variable. La producción de NO en estómago disminuyó 60%, mientras que la producción de MDA aumentó 55% después del protocolo. El promedio de la concentración plasmática de MDA aumentó después de 96 horas de iniciar el protocolo. No hubo diferencias significativas en el promedio de NO plasmático durante el protocolo. Conclusión: el protocolo utilizado para inducir úlceras gástricas produce disminución en la citoprotección derivada del NO e induce estrés oxidativo en la mucosa gástrica.COL0001262application/pdfengUniversidad de Antioquia, Facultad de Ciencias AgrariasCENTAUROMedellín, Colombiainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_2df8fbb1https://purl.org/redcol/resource_type/ARTArtículo de investigaciónhttp://purl.org/coar/version/c_970fb48d4fbd8a85Atribución-NoComercial-CompartirIgual 2.5 Colombia (CC BY-NC-SA 2.5 CO)info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/co/http://purl.org/coar/access_right/c_abf2https://creativecommons.org/licenses/by-nc-sa/4.0/Nitric oxide and malondialdehyde concentration in gastric contents and blood in an equine model of gastric ulcer induced by phenylbutazoneConcentración de óxido nítrico y malonaldehído en contenido gástrico y en sangre en un modelo de úlcera gástrica equina inducida por fenilbutazonaRev. Colomb. Cienc. Pecu.Revista Colombiana de Ciencias Pecuarias4350291ORIGINALZuluaga_A_2016_Nitric_oxide_malondialdehyde.pdfZuluaga_A_2016_Nitric_oxide_malondialdehyde.pdfArtículo de investigaciónapplication/pdf1182893http://bibliotecadigital.udea.edu.co/bitstream/10495/8057/1/Zuluaga_A_2016_Nitric_oxide_malondialdehyde.pdf2b61422ce4d16cc83e45871ed935a36cMD51CC-LICENSElicense_urllicense_urltext/plain; charset=utf-849http://bibliotecadigital.udea.edu.co/bitstream/10495/8057/2/license_url4afdbb8c545fd630ea7db775da747b2fMD52license_textlicense_texttext/html; charset=utf-80http://bibliotecadigital.udea.edu.co/bitstream/10495/8057/3/license_textd41d8cd98f00b204e9800998ecf8427eMD53license_rdflicense_rdfapplication/rdf+xml; charset=utf-80http://bibliotecadigital.udea.edu.co/bitstream/10495/8057/4/license_rdfd41d8cd98f00b204e9800998ecf8427eMD54LICENSElicense.txtlicense.txttext/plain; charset=utf-81748http://bibliotecadigital.udea.edu.co/bitstream/10495/8057/5/license.txt8a4605be74aa9ea9d79846c1fba20a33MD5510495/8057oai:bibliotecadigital.udea.edu.co:10495/80572021-05-21 20:28:35.684Repositorio Institucional Universidad de Antioquiaandres.perez@udea.edu.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 |