Molecular cloning and characterization of two hsp 70 homologous genes from the dimorphic fungus Paracoccidioides brasiliensis
ABSTRACT : Paracoccidioides brasiliensis, a dimorphic fungus, is the etiologic agent of Paracoccidioidomycosis (PCM), one of the most important systemic mycosis in Latin America. Two genes (2.2 and 1DB5) were cloned, characterized and sequenced; they showed homology with members of hsp70 gene family...
- Autores:
-
Floréz Escobar, Alvaro Mauricio
Oviedo, Adona
Cardona, Astrid
Herrera, Mónica
García Rojas, Edwin
Restrepo Moreno, Ángela
McEwen Ochoa, Juan Guillermo
- Tipo de recurso:
- Article of investigation
- Fecha de publicación:
- 2003
- Institución:
- Universidad de Antioquia
- Repositorio:
- Repositorio UdeA
- Idioma:
- eng
- OAI Identifier:
- oai:bibliotecadigital.udea.edu.co:10495/29307
- Acceso en línea:
- http://hdl.handle.net/10495/29307
- Palabra clave:
- Proteínas de Choque Térmico
Heat-Shock Proteins
Paracoccidioides
Genes
Paracoccidioides brasiliensis
- Rights
- openAccess
- License
- http://creativecommons.org/licenses/by-nc-nd/2.5/co/
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| dc.title.spa.fl_str_mv |
Molecular cloning and characterization of two hsp 70 homologous genes from the dimorphic fungus Paracoccidioides brasiliensis |
| dc.title.alternative.spa.fl_str_mv |
Clonaje y caracterización molecular de dos genes homólogos hsp70 del hongo dimórfico Paracoccidioides brasilensis. |
| title |
Molecular cloning and characterization of two hsp 70 homologous genes from the dimorphic fungus Paracoccidioides brasiliensis |
| spellingShingle |
Molecular cloning and characterization of two hsp 70 homologous genes from the dimorphic fungus Paracoccidioides brasiliensis Proteínas de Choque Térmico Heat-Shock Proteins Paracoccidioides Genes Paracoccidioides brasiliensis |
| title_short |
Molecular cloning and characterization of two hsp 70 homologous genes from the dimorphic fungus Paracoccidioides brasiliensis |
| title_full |
Molecular cloning and characterization of two hsp 70 homologous genes from the dimorphic fungus Paracoccidioides brasiliensis |
| title_fullStr |
Molecular cloning and characterization of two hsp 70 homologous genes from the dimorphic fungus Paracoccidioides brasiliensis |
| title_full_unstemmed |
Molecular cloning and characterization of two hsp 70 homologous genes from the dimorphic fungus Paracoccidioides brasiliensis |
| title_sort |
Molecular cloning and characterization of two hsp 70 homologous genes from the dimorphic fungus Paracoccidioides brasiliensis |
| dc.creator.fl_str_mv |
Floréz Escobar, Alvaro Mauricio Oviedo, Adona Cardona, Astrid Herrera, Mónica García Rojas, Edwin Restrepo Moreno, Ángela McEwen Ochoa, Juan Guillermo |
| dc.contributor.author.none.fl_str_mv |
Floréz Escobar, Alvaro Mauricio Oviedo, Adona Cardona, Astrid Herrera, Mónica García Rojas, Edwin Restrepo Moreno, Ángela McEwen Ochoa, Juan Guillermo |
| dc.subject.decs.none.fl_str_mv |
Proteínas de Choque Térmico Heat-Shock Proteins Paracoccidioides Genes |
| topic |
Proteínas de Choque Térmico Heat-Shock Proteins Paracoccidioides Genes Paracoccidioides brasiliensis |
| dc.subject.proposal.spa.fl_str_mv |
Paracoccidioides brasiliensis |
| description |
ABSTRACT : Paracoccidioides brasiliensis, a dimorphic fungus, is the etiologic agent of Paracoccidioidomycosis (PCM), one of the most important systemic mycosis in Latin America. Two genes (2.2 and 1DB5) were cloned, characterized and sequenced; they showed homology with members of hsp70 gene family. By using several probe fragments derived from these genes, levels of expression for each gene were determined by Northern blot during transition to the yeast phase. The highest level of hsp70 transcript occurred between 30 min to 6 hours after temperature shift, with significant reduction after 36-48 hours. However, after 72 hours, the level of the transcription increased until yeast phase was reached. As a response to temperature increase, hsp 70 genes are expressed during the transition phase and possibly play a role in the differentiation process. |
| publishDate |
2003 |
| dc.date.issued.none.fl_str_mv |
2003 |
| dc.date.accessioned.none.fl_str_mv |
2022-06-19T20:36:26Z |
| dc.date.available.none.fl_str_mv |
2022-06-19T20:36:26Z |
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info:eu-repo/semantics/article |
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http://purl.org/coar/version/c_970fb48d4fbd8a85 |
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info:eu-repo/semantics/publishedVersion |
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http://purl.org/coar/resource_type/c_2df8fbb1 |
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https://purl.org/redcol/resource_type/ART |
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Artículo de investigación |
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http://purl.org/coar/resource_type/c_2df8fbb1 |
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publishedVersion |
| dc.identifier.citation.spa.fl_str_mv |
Floréz AM, Oviedo A, Cardona A, Herrera M, García E, Restrepo A, McEwen JG. Clonaje y caracterización molecular de dos genes homólogos hsp70 del hongo dimórficoParacoccidioides brasilensis. biomedica [Internet]. 1 de diciembre de 2003 [citado 19 de junio de 2022];23(4):424-36. Disponible en: https://revistabiomedica.org/index.php/biomedica/article/view/1237 |
| dc.identifier.issn.none.fl_str_mv |
0120-4157 |
| dc.identifier.uri.none.fl_str_mv |
http://hdl.handle.net/10495/29307 |
| dc.identifier.doi.none.fl_str_mv |
10.7705/biomedica.v23i4.1237 |
| dc.identifier.eissn.none.fl_str_mv |
2590-7379 |
| identifier_str_mv |
Floréz AM, Oviedo A, Cardona A, Herrera M, García E, Restrepo A, McEwen JG. Clonaje y caracterización molecular de dos genes homólogos hsp70 del hongo dimórficoParacoccidioides brasilensis. biomedica [Internet]. 1 de diciembre de 2003 [citado 19 de junio de 2022];23(4):424-36. Disponible en: https://revistabiomedica.org/index.php/biomedica/article/view/1237 0120-4157 10.7705/biomedica.v23i4.1237 2590-7379 |
| url |
http://hdl.handle.net/10495/29307 |
| dc.language.iso.spa.fl_str_mv |
eng |
| language |
eng |
| dc.relation.ispartofjournalabbrev.spa.fl_str_mv |
Biomédica |
| dc.rights.spa.fl_str_mv |
info:eu-repo/semantics/openAccess |
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http://creativecommons.org/licenses/by-nc-nd/2.5/co/ |
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openAccess |
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http://creativecommons.org/licenses/by-nc-nd/2.5/co/ http://purl.org/coar/access_right/c_abf2 https://creativecommons.org/licenses/by-nc-nd/4.0/ |
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13 |
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application/pdf |
| dc.publisher.spa.fl_str_mv |
Instituto Nacional de Salud |
| dc.publisher.group.spa.fl_str_mv |
Micología Médica y Experimental Biología Celular y Molecular CIB, U. de A. U. del Rosario |
| dc.publisher.place.spa.fl_str_mv |
Bogotá, Colombia |
| institution |
Universidad de Antioquia |
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andres.perez@udea.edu.co |
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Floréz Escobar, Alvaro MauricioOviedo, AdonaCardona, AstridHerrera, MónicaGarcía Rojas, EdwinRestrepo Moreno, ÁngelaMcEwen Ochoa, Juan Guillermo2022-06-19T20:36:26Z2022-06-19T20:36:26Z2003Floréz AM, Oviedo A, Cardona A, Herrera M, García E, Restrepo A, McEwen JG. Clonaje y caracterización molecular de dos genes homólogos hsp70 del hongo dimórficoParacoccidioides brasilensis. biomedica [Internet]. 1 de diciembre de 2003 [citado 19 de junio de 2022];23(4):424-36. Disponible en: https://revistabiomedica.org/index.php/biomedica/article/view/12370120-4157http://hdl.handle.net/10495/2930710.7705/biomedica.v23i4.12372590-7379ABSTRACT : Paracoccidioides brasiliensis, a dimorphic fungus, is the etiologic agent of Paracoccidioidomycosis (PCM), one of the most important systemic mycosis in Latin America. Two genes (2.2 and 1DB5) were cloned, characterized and sequenced; they showed homology with members of hsp70 gene family. By using several probe fragments derived from these genes, levels of expression for each gene were determined by Northern blot during transition to the yeast phase. The highest level of hsp70 transcript occurred between 30 min to 6 hours after temperature shift, with significant reduction after 36-48 hours. However, after 72 hours, the level of the transcription increased until yeast phase was reached. As a response to temperature increase, hsp 70 genes are expressed during the transition phase and possibly play a role in the differentiation process.RESUMEN : Paracoccidiodes brasiliensis es el agente etiológico de la Paracoccidioidomicosis (PCM), una de las micosis sistémicas mas importantes en Latinoamérica. Dos genes hsp70 (Clones 2.2 y 1DB5) fueron clonados, caracterizados y secuenciados. El análisis de secuencia mostró que los clones 2.2 y 1DB5 poseen una alta homología con la familia de genes de choque térmico hsp70. Mediante la utilización de fragmentos de ADN provenientes de ambos clones, se determinó la expresión por Northern blot durante la fase de transición a levadura. Nosotros encontramos los niveles mas altos de transcriptos ocurre entre los 30 min y 6 h después de cambiar la temperatura a 37°C. Entre las 36 y 48 h, se reducen los niveles de ARNm. Sin embargo, los niveles de ARNm aumentan a las 72 h hasta alcanzar la fase de levadura. Como resultado del aumento de la temperatura durante la fase de transición micelio a levadura, se incrementa la expresión de genes hsp70 sugiriendo que las proteínas de choque térmico tienen un papel durante el proceso de diferenciación.COL0013709COL000096213application/pdfengInstituto Nacional de SaludMicología Médica y ExperimentalBiología Celular y Molecular CIB, U. de A. U. del RosarioBogotá, Colombiainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_2df8fbb1https://purl.org/redcol/resource_type/ARTArtículo de investigaciónhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-nd/2.5/co/http://purl.org/coar/access_right/c_abf2https://creativecommons.org/licenses/by-nc-nd/4.0/Molecular cloning and characterization of two hsp 70 homologous genes from the dimorphic fungus Paracoccidioides brasiliensisClonaje y caracterización molecular de dos genes homólogos hsp70 del hongo dimórfico Paracoccidioides brasilensis.Proteínas de Choque TérmicoHeat-Shock ProteinsParacoccidioidesGenesParacoccidioides brasiliensisBiomédicaBiomédica424436234ORIGINALMcEwenJuan_2003_Molecular_Cloning.pdfMcEwenJuan_2003_Molecular_Cloning.pdfArtículo de investigaciónapplication/pdf228530https://bibliotecadigital.udea.edu.co/bitstream/10495/29307/1/McEwenJuan_2003_Molecular_Cloning.pdf3a0b0228058e957714e599c95118091cMD51CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8823https://bibliotecadigital.udea.edu.co/bitstream/10495/29307/2/license_rdfb88b088d9957e670ce3b3fbe2eedbc13MD52LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://bibliotecadigital.udea.edu.co/bitstream/10495/29307/3/license.txt8a4605be74aa9ea9d79846c1fba20a33MD5310495/29307oai:bibliotecadigital.udea.edu.co:10495/293072022-06-19 16:35:55.168Repositorio Institucional Universidad de Antioquiaandres.perez@udea.edu.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 |